Rw5G048900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000668
Physical Location & Seq
Forward (+)
84945317 .. 84946867
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048900.1

Sequence Viewer

Length: 1467 bp
ATGAAGACTAAAAAGTCAAGTAAGAAGGGCGCAGCACGGGAGCTAGATAGTCCGGCTATTAAAGACAAGGTTGACATTGGTGTTTTGAGTGGATGGGAAAAGAGATCATCGGAAGTACCACCCTCCGATGGTACTCCTATTCACTCAACAAGATCAAAATCCACAAATGTTGTTAACATTGATGTTCTCCCTGACTTTCTGTTGGTTGAAATCCTATGTCGGCTTCCTAGCAAGGAATTCGTTTTCCAATGCAAATGTGTTTCCAAGCATTGGCTCAATCTCATCTCAGATCCGTATTTGATTGGCCGCTTTCTACATCTTCAAAGATTTCATAAGCAAACCCCAATAGCACATTCTCTGATAGACGAAAGAGGGGAGGAATTCCCCACTCCTAGAACAACACAGTCGTCATCCAATCTGCTAGCTCCCCTGTTCGAAATACTCATGGGTTTCCACGGTCTGAAAGAAAAGCCATATATGGTAGGAACATGCAATGATTTAGTTTTGTGCTGCCGAACTCGACATTACCAAGACCAACGTGATTTCTACATTTGCAATCCATGCACAAGGCAATGGATTGCTCTTCCTCCCACCACTCGACGTCGCACGAATATACCAATTGGATTCATCTGTGATCGCTACTATGAGGAGGAAAATAGTAAAAGTGGTACCAATAGTACTGTTAATATTAAAAAGGACTACAGGTGCAAGGTTGTGAGAATTATTCCTCACGATTTATCTGAATTAGGTAATTCTTTTTTCACTCATTTTTCCAAATTCATTGTTGAGGTATTCTCTTTCGACACTGGTGAATGGAGAGAGTCAATTGTATCATCCCCACGAAGCATTCGGTTTGGACCTGCTACTAGCGTTGCTTACAATGGAATGTTGTATTGGACATATTGGCAATATGACCCGACCGTTGACCAGATAGGGCTTATTGGGTTGGATCCTTTTGTGATCGGCAATAGCAGTGCTAGCCCTAGTGGTGATGAGACTGTTGACGATGATCATTACCAATTTCGTCTCTTTGATGCAGTTTATTGTGGTTTTGGTTTCGTCGTGCCATGGATATGTATGCGCCGAGGGCGTCTGCAAATGTGGGGCTACAATTACTGGAACGCTGATTTGACGGTTTGGGAGTTAACAGGTGATGATGATCTTGGATCAGCTGACGAGTTCTATTTGAAAAATGTGAGTGTTTTTCGAGTAACCACCTGTGAAGTTTCTGAAAATAATGTTGTTGTTCTAGCCTTGGACCCGAATGATGAGGATATCTTTTATGTAAAAGTGTATGATGGCTGTTTCCGGACCAAATACTATTTCATGTACAACATTTGTACAAGAAAGTGGTCAAAAGTAGCTAGCACCAAGCATGTTGAAGACGATCATCTCTTTCGATTGGTGCTCCCATGGTGGCCAACACCACTTCGTAGACTACCTGTGGTTGAAGAATTACCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

488

Amino Acids

56.43

Weight (kDa)

6.47

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 62 - 100 1.8e-06 F-box domain
b-prop_At3g26010-like PF24750 157 - 317 4.5e-08 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AatII GACGTC 1 cut(s) 604
Acc36I ACCTGC 1 cut(s) 868
Acc65I GGTACC 1 cut(s) 668
AccB1I GGYRCC 1 cut(s) 668
AccB7I CCANNNNNTGG 1 cut(s) 270
AccI GTMKAC 1 cut(s) 1435
AccIII TCCGGA 1 cut(s) 1308
AciI CCGC 1 cut(s) 307
AclWI GGATC 4 cut(s) 284, 944, 957, 1174
AcoI YGGCCR 2 cut(s) 304, 1418
AcsI RAATTY 3 cut(s) 236, 380, 776
AcyI GRCGYC 2 cut(s) 601, 1090
AfaI GTAC 6 cut(s) 117, 133, 670, 679, 1331, 1342
AfiI CCNNNNNNNGG 2 cut(s) 128, 270
AgsI TTSAA 5 cut(s) 209, 323, 1189, 1382, 1451
AjuI GAANNNNNNNTTGG 6 cut(s) 337, 369, 877, 909, 1307, 1339
AluBI AGCT 4 cut(s) 43, 425, 1172, 1364
AluI AGCT 4 cut(s) 43, 425, 1172, 1364
Alw21I GWGCWC 1 cut(s) 1410
Alw26I GTCTC 2 cut(s) 989, 1031
AlwI GGATC 4 cut(s) 284, 944, 957, 1174
Aor13HI TCCGGA 1 cut(s) 1308
AoxI GGCC 2 cut(s) 304, 1418
ApeKI GCWGC 2 cut(s) 32, 510
ApoI RAATTY 3 cut(s) 236, 380, 776
Asp718I GGTACC 1 cut(s) 668
AspLEI GCGC 2 cut(s) 32, 1083
AspS9I GGNCC 3 cut(s) 857, 1258, 1311
AsuHPI GGTGA 3 cut(s) 821, 1001, 1163
AsuII TTCGAA 1 cut(s) 435
AsuNHI GCTAGC 3 cut(s) 421, 977, 1364
AvaII GGWCC 3 cut(s) 857, 1258, 1311
BalI TGGCCA 1 cut(s) 1420
BamHI GGATCC 1 cut(s) 949
BanI GGYRCC 1 cut(s) 668
BbsI GAAGAC 2 cut(s) 11, 1389
Bbv12I GWGCWC 1 cut(s) 1410
BbvI GCAGC 2 cut(s) 44, 497
BccI CCATC 3 cut(s) 87, 122, 1292
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BclI TGATCA 1 cut(s) 1009
BcoDI GTCTC 2 cut(s) 989, 1031
BfaI CTAG 9 cut(s) 44, 228, 393, 422, 867, 978, 984, 1250, 1365
BfmI CTRYAG 1 cut(s) 700
BfuAI ACCTGC 1 cut(s) 868
BisI GCNGC 3 cut(s) 33, 307, 511
BlsI GCNGC 3 cut(s) 34, 308, 512
BmcAI AGTACT 1 cut(s) 679
Bme18I GGWCC 3 cut(s) 857, 1258, 1311
BmgT120I GGNCC 3 cut(s) 857, 1258, 1311
BmiI GGNNCC 3 cut(s) 670, 951, 1260
BmsI GCATC 1 cut(s) 1024
BmtI GCTAGC 3 cut(s) 425, 981, 1368
BpiI GAAGAC 2 cut(s) 11, 1389
BplI GAGNNNNNCTC 2 cut(s) 779, 811
Bpu14I TTCGAA 1 cut(s) 435
BsaBI GATNNNNATC 2 cut(s) 157, 1158
BsaHI GRCGYC 2 cut(s) 601, 1090
BsaJI CCNNGG 5 cut(s) 454, 1067, 1084, 1254, 1412
BsaWI WCCGGW 1 cut(s) 1308
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 270
Bse1I ACTGG 2 cut(s) 811, 1121
Bse3DI GCAATG 2 cut(s) 499, 578
Bse8I GATNNNNATC 2 cut(s) 157, 1158
BseAI TCCGGA 1 cut(s) 1308
BseDI CCNNGG 5 cut(s) 454, 1067, 1084, 1254, 1412
BseGI GGATG 3 cut(s) 98, 410, 833
BseJI GATNNNNATC 2 cut(s) 157, 1158
BseLI CCNNNNNNNGG 2 cut(s) 128, 270
BseMI GCAATG 2 cut(s) 499, 578
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 2 cut(s) 811, 1121
BseRI GAGGAG 1 cut(s) 662
BseXI GCAGC 2 cut(s) 44, 497
Bsh1285I CGRYCG 1 cut(s) 921
BshFI GGCC 2 cut(s) 306, 1420
BshNI GGYRCC 1 cut(s) 668
BsiEI CGRYCG 1 cut(s) 921
BsiHKAI GWGCWC 1 cut(s) 1410
BsiSI CCGG 2 cut(s) 53, 1309
BslI CCNNNNNNNGG 2 cut(s) 128, 270
BsmAI GTCTC 2 cut(s) 989, 1031
BsmBI CGTCTC 1 cut(s) 1031
BsmI GAATGC 1 cut(s) 846
BsnI GGCC 2 cut(s) 306, 1420
Bsp119I TTCGAA 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 1410
Bsp13I TCCGGA 1 cut(s) 1308
Bsp1407I TGTACA 2 cut(s) 1329, 1340
Bsp19I CCATGG 2 cut(s) 1067, 1412
BspACI CCGC 1 cut(s) 307
BspANI GGCC 2 cut(s) 306, 1420
BspCNI CTCAG 1 cut(s) 299
BspEI TCCGGA 1 cut(s) 1308
BspLI GGNNCC 3 cut(s) 670, 951, 1260
BspMI ACCTGC 1 cut(s) 868
BspOI GCTAGC 3 cut(s) 425, 981, 1368
BspPI GGATC 4 cut(s) 284, 944, 957, 1174
BspQI GCTCTTC 1 cut(s) 588
BspT104I TTCGAA 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 668
BsrDI GCAATG 2 cut(s) 499, 578
BsrGI TGTACA 2 cut(s) 1329, 1340
BsrI ACTGG 2 cut(s) 811, 1121
BssECI CCNNGG 5 cut(s) 454, 1067, 1084, 1254, 1412
BssNI GRCGYC 2 cut(s) 601, 1090
BssT1I CCWWGG 3 cut(s) 1067, 1254, 1412
Bst4CI ACNGT 6 cut(s) 405, 458, 682, 922, 1000, 1135
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 2 cut(s) 601, 1090
BstAPI GCANNNNNTGC 1 cut(s) 561
BstAUI TGTACA 2 cut(s) 1329, 1340
BstBI TTCGAA 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 423, 979, 1366
BstDEI CTNAG 1 cut(s) 286
BstDSI CCRYGG 3 cut(s) 454, 1067, 1412
BstF5I GGATG 3 cut(s) 98, 410, 833
BstHHI GCGC 2 cut(s) 32, 1083
BstMAI GTCTC 2 cut(s) 989, 1031
BstMCI CGRYCG 1 cut(s) 921
BstMWI GCNNNNNNNGC 3 cut(s) 561, 978, 1087
BstNSI RCATGY 2 cut(s) 492, 1379
BstSFI CTRYAG 1 cut(s) 700
BstV1I GCAGC 2 cut(s) 44, 497
BstV2I GAAGAC 2 cut(s) 11, 1389
BstX2I RGATCY 2 cut(s) 289, 949
BstYI RGATCY 2 cut(s) 289, 949
BsuRI GGCC 2 cut(s) 306, 1420
BtgI CCRYGG 3 cut(s) 454, 1067, 1412
BtsCI GGATG 3 cut(s) 98, 410, 833
BtsI GCAGTG 1 cut(s) 979
BtsIMutI CAGTG 2 cut(s) 804, 979
BveI ACCTGC 1 cut(s) 868
Cac8I GCNNGC 3 cut(s) 423, 979, 1366
CfoI GCGC 2 cut(s) 32, 1083
Cfr13I GGNCC 3 cut(s) 857, 1258, 1311
CseI GACGC 1 cut(s) 1079
Csp6I GTAC 6 cut(s) 116, 132, 669, 678, 1330, 1341
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 7 cut(s) 445, 489, 561, 1068, 1327, 1376, 1413
CviQI GTAC 6 cut(s) 116, 132, 669, 678, 1330, 1341
DdeI CTNAG 1 cut(s) 286
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
EaeI YGGCCR 2 cut(s) 304, 1418
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco130I CCWWGG 3 cut(s) 1067, 1254, 1412
Eco32I GATATC 1 cut(s) 1276
Eco47I GGWCC 3 cut(s) 857, 1258, 1311
EcoRI GAATTC 2 cut(s) 236, 380
EcoRV GATATC 1 cut(s) 1276
EcoT14I CCWWGG 3 cut(s) 1067, 1254, 1412
ErhI CCWWGG 3 cut(s) 1067, 1254, 1412
Esp3I CGTCTC 1 cut(s) 1031
FaeI CATG 7 cut(s) 448, 492, 564, 1071, 1330, 1379, 1416
FatI CATG 7 cut(s) 444, 488, 560, 1067, 1326, 1375, 1412
FbaI TGATCA 1 cut(s) 1009
FblI GTMKAC 1 cut(s) 1435
Fnu4HI GCNGC 3 cut(s) 33, 307, 511
FokI GGATG 3 cut(s) 105, 397, 820
Fsp4HI GCNGC 3 cut(s) 33, 307, 511
FspBI CTAG 9 cut(s) 44, 228, 393, 422, 867, 978, 984, 1250, 1365
GlaI GCGC 2 cut(s) 31, 1082
GluI GCNGC 3 cut(s) 33, 307, 511
HaeIII GGCC 2 cut(s) 306, 1420
HapII CCGG 2 cut(s) 53, 1309
HgaI GACGC 1 cut(s) 1079
HhaI GCGC 2 cut(s) 32, 1083
Hin1I GRCGYC 2 cut(s) 601, 1090
Hin1II CATG 7 cut(s) 448, 492, 564, 1071, 1330, 1379, 1416
Hin6I GCGC 2 cut(s) 30, 1081
HinP1I GCGC 2 cut(s) 30, 1081
HincII GTYRAC 5 cut(s) 73, 175, 925, 1003, 1146
HindII GTYRAC 5 cut(s) 73, 175, 925, 1003, 1146
HinfI GANTC 2 cut(s) 624, 821
HpaI GTTAAC 2 cut(s) 175, 1146
HpaII CCGG 2 cut(s) 53, 1309
HphI GGTGA 3 cut(s) 821, 1001, 1163
Hpy166II GTNNAC 6 cut(s) 73, 175, 925, 1003, 1146, 1436
Hpy188I TCNGA 7 cut(s) 112, 127, 289, 360, 462, 742, 1231
Hpy188III TCNNGA 2 cut(s) 731, 1309
Hpy8I GTNNAC 6 cut(s) 73, 175, 925, 1003, 1146, 1436
Hpy99I CGWCG 3 cut(s) 603, 606, 1064
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 6 cut(s) 405, 458, 682, 922, 1000, 1135
HpyCH4IV ACGT 2 cut(s) 538, 601
HpyCH4V TGCA 7 cut(s) 252, 492, 555, 564, 708, 1037, 1096
HpyF10VI GCNNNNNNNGC 3 cut(s) 561, 978, 1087
HpyF3I CTNAG 1 cut(s) 286
HpySE526I ACGT 2 cut(s) 538, 601
Hsp92I GRCGYC 2 cut(s) 601, 1090
Hsp92II CATG 7 cut(s) 448, 492, 564, 1071, 1330, 1379, 1416
HspAI GCGC 2 cut(s) 30, 1081
Kpn2I TCCGGA 1 cut(s) 1308
KpnI GGTACC 1 cut(s) 672
Ksp22I TGATCA 1 cut(s) 1009
KspAI GTTAAC 2 cut(s) 175, 1146
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 3 cut(s) 40, 430, 1413
Lsp1109I GCAGC 2 cut(s) 44, 497
LweI GCATC 1 cut(s) 1024
MaeI CTAG 9 cut(s) 44, 228, 393, 422, 867, 978, 984, 1250, 1365
MaeII ACGT 2 cut(s) 538, 601
MaeIII GTNAC 1 cut(s) 1210
MboII GAAGA 5 cut(s) 16, 311, 575, 1394, 1463
MfeI CAATTG 2 cut(s) 618, 825
MflI RGATCY 2 cut(s) 289, 949
MhlI GDGCHC 1 cut(s) 1410
MlsI TGGCCA 1 cut(s) 1420
MluNI TGGCCA 1 cut(s) 1420
MlyI GAGTC 1 cut(s) 830
MmeI TCCRAC 1 cut(s) 927
Mox20I TGGCCA 1 cut(s) 1420
MroI TCCGGA 1 cut(s) 1308
MscI TGGCCA 1 cut(s) 1420
MseI TTAA 5 cut(s) 60, 174, 684, 690, 1145
MslI CAYNNNNRTG 1 cut(s) 1072
Msp20I TGGCCA 1 cut(s) 1420
MspA1I CMGCKG 1 cut(s) 1172
MspI CCGG 2 cut(s) 53, 1309
MunI CAATTG 2 cut(s) 618, 825
Mva1269I GAATGC 1 cut(s) 846
MwoI GCNNNNNNNGC 3 cut(s) 561, 978, 1087
NcoI CCATGG 2 cut(s) 1067, 1412
NheI GCTAGC 3 cut(s) 421, 977, 1364
NlaIII CATG 7 cut(s) 448, 492, 564, 1071, 1330, 1379, 1416
NlaIV GGNNCC 3 cut(s) 670, 951, 1260
NmeAIII GCCGAG 1 cut(s) 1109
NspI RCATGY 2 cut(s) 492, 1379
NspV TTCGAA 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 588
PctI GAATGC 1 cut(s) 846
PfeI GAWTC 1 cut(s) 624
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 3 cut(s) 34, 308, 512
PleI GAGTC 1 cut(s) 829
PpsI GAGTC 1 cut(s) 829
PspN4I GGNNCC 3 cut(s) 670, 951, 1260
PspPI GGNCC 3 cut(s) 857, 1258, 1311
PsuI RGATCY 2 cut(s) 289, 949
PvuII CAGCTG 1 cut(s) 1172
RsaI GTAC 6 cut(s) 117, 133, 670, 679, 1331, 1342
RsaNI GTAC 6 cut(s) 116, 132, 669, 678, 1330, 1341
RseI CAYNNNNRTG 1 cut(s) 1072
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 5 cut(s) 60, 174, 684, 690, 1145
SatI GCNGC 3 cut(s) 33, 307, 511
Sau96I GGNCC 3 cut(s) 857, 1258, 1311
ScaI AGTACT 1 cut(s) 679
SchI GAGTC 1 cut(s) 830
SduI GDGCHC 1 cut(s) 1410
SfaNI GCATC 1 cut(s) 1024
SfcI CTRYAG 1 cut(s) 700
SfuI TTCGAA 1 cut(s) 435
SinI GGWCC 3 cut(s) 857, 1258, 1311
SmiMI CAYNNNNRTG 1 cut(s) 1072
SsiI CCGC 1 cut(s) 307
SspI AATATT 1 cut(s) 688
SspMI CTAG 9 cut(s) 44, 228, 393, 422, 867, 978, 984, 1250, 1365
StyI CCWWGG 3 cut(s) 1067, 1254, 1412
TaaI ACNGT 6 cut(s) 405, 458, 682, 922, 1000, 1135
TaiI ACGT 2 cut(s) 541, 604
TaqI TCGA 6 cut(s) 435, 520, 598, 801, 1207, 1399
TatI WGTACW 3 cut(s) 677, 1329, 1340
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 1 cut(s) 624
Tru1I TTAA 5 cut(s) 60, 174, 684, 690, 1145
Tru9I TTAA 5 cut(s) 60, 174, 684, 690, 1145
TscAI CASTG 2 cut(s) 811, 979
TseI GCWGC 2 cut(s) 32, 510
TspDTI ATGAA 5 cut(s) 17, 320, 616, 769, 1315
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 2 cut(s) 811, 979
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 3 cut(s) 857, 1258, 1311
XapI RAATTY 3 cut(s) 236, 380, 776
XceI RCATGY 2 cut(s) 492, 1379
XmiI GTMKAC 1 cut(s) 1435
XspI CTAG 9 cut(s) 44, 228, 393, 422, 867, 978, 984, 1250, 1365
ZraI GACGTC 1 cut(s) 602
ZrmI AGTACT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.