FvH4_5g12451

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000960
Physical Location & Seq
Reverse (-)
7012725 .. 7014376
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12451.t1

Sequence Viewer

Length: 1068 bp
ATGGGACGTCCTGAGGCGGATCAAGATCATCAACCGTCGAGTTCCAACCAGGCATGTCATCCAACACATTGCGAATTACAAGATTCCGAGAAGCCTGAACAACAAGTTTCGTCTCCAAACCCCAGTAATATCCAGACCTATGTTTTCAGGGCAGAAAATGATCATGAGCATCAAGAACAAGGCCACACCCGAACGTCCTCCACTAATTCCCAAGAAAGTCAAGCAATTCAAACGCATCAACAAGCACCGCCGTTGCCTTATGATCACAACGTGCACTACTATTTCCCAACGCCGAATAACCCCCAGCCTCCACACCCTTTCCAGGCAATGCCTCCAGGTTTACGGCCACATCCGACCTCCTTATATCCTCCTCCTTCTCCTCCTCCTCCTTCTCTTTATCAGTCTCAACCTTTAAACAACTATAGGCCTCCTCCTCAACCTTTGCACAATTTTTATAGTGGATCACAGCAAGTAGTAATGTACCCTCCTCCAGCTGCCTACAATTATCGTTCTAATTCTTTAAATCCCCAGCCCTATGTAAATTACAATAATTCTGCAGCAACTGGTATACCCATGATGCAAAATATACAATACGTACCGGTGCTCATGCCACAGTCAGGTAGCAGCGGCGAGGGTACTACTTGGAAAACTGGGCTATGCGATTGCTTTCTTGACCCCCCAAATGCTATCATGACAATGATATGCCCGTGTTGGACGTTTGGCCAGATTGCAGAGATTGTAGATAACGGCCAAACCTCTTGTAGTTGTAATGCTTTACTATACATGATCATCTGGTTCTTCATCATGGTGCCATGCTTGTTGTCCTGCACATACCGGAGAAAACTAAGGAACAAGCTTGACCTGCCGGAAAGCCCCGGTCCCGACTGCATCATCCACGTCTTATGCGAGCTGTGTGCTCTGTGCCAAGAACACAGGGAGCTTGAGCTCAGAGGCCTCGACCCTTCTTTAGGATGGGTAGGAAACATGGAGCAACTTCAGAGGCTGCAGAAAAAGAGGCAAGGTACAGTGGTCACACCTCCAATGCCTCAGAGAATGACGGGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

356

Amino Acids

39.97

Weight (kDa)

6.03

Isoelectric Point (pI)

73.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 215 - 313 1.5e-28 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 870
AccB1I GGYRCC 1 cut(s) 808
AccI GTMKAC 1 cut(s) 568
AciI CCGC 3 cut(s) 17, 248, 627
AclWI GGATC 2 cut(s) 27, 469
AcoI YGGCCR 3 cut(s) 344, 721, 748
AcuI CTGAAG 1 cut(s) 980
AcyI GRCGYC 1 cut(s) 7
AdeI CACNNNGTG 1 cut(s) 271
AfaI GTAC 4 cut(s) 482, 597, 637, 1024
AfiI CCNNNNNNNGG 3 cut(s) 322, 617, 968
AgeI ACCGGT 1 cut(s) 598
AgsI TTSAA 1 cut(s) 230
AjiI CACGTC 1 cut(s) 898
AjnI CCWGG 3 cut(s) 48, 321, 334
AluBI AGCT 5 cut(s) 494, 856, 910, 940, 946
AluI AGCT 5 cut(s) 494, 856, 910, 940, 946
Alw21I GWGCWC 4 cut(s) 276, 606, 919, 948
Alw26I GTCTC 2 cut(s) 117, 408
Alw44I GTGCAC 1 cut(s) 272
AlwI GGATC 2 cut(s) 27, 469
AlwNI CAGNNNCTG 2 cut(s) 563, 1003
AoxI GGCC 6 cut(s) 181, 344, 425, 721, 748, 952
ApaLI GTGCAC 1 cut(s) 272
ApeKI GCWGC 4 cut(s) 494, 557, 624, 1003
AsiGI ACCGGT 1 cut(s) 598
AspS9I GGNCC 1 cut(s) 878
AsuC2I CCSGG 1 cut(s) 876
AvaII GGWCC 1 cut(s) 878
AxyI CCTNAGG 1 cut(s) 12
BaeGI GKGCMC 1 cut(s) 276
BaeI ACNNNNGTAYC 2 cut(s) 1014, 1047
BalI TGGCCA 1 cut(s) 723
BanI GGYRCC 1 cut(s) 808
BanII GRGCYC 1 cut(s) 948
Bbv12I GWGCWC 4 cut(s) 276, 606, 919, 948
BbvI GCAGC 4 cut(s) 481, 569, 636, 990
BccI CCATC 1 cut(s) 966
BceAI ACGGC 3 cut(s) 235, 359, 763
BcgI CGANNNNNNTGC 2 cut(s) 896, 930
BciT130I CCWGG 3 cut(s) 50, 323, 336
BclI TGATCA 3 cut(s) 160, 262, 786
BcnI CCSGG 1 cut(s) 876
BcoDI GTCTC 2 cut(s) 117, 408
BfmI CTRYAG 3 cut(s) 421, 555, 1004
BfuAI ACCTGC 1 cut(s) 870
BisI GCNGC 5 cut(s) 495, 558, 625, 628, 1004
BlsI GCNGC 5 cut(s) 496, 559, 626, 629, 1005
Bme1390I CCNGG 4 cut(s) 50, 323, 336, 876
Bme18I GGWCC 1 cut(s) 878
BmgBI CACGTC 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 878
BmiI GGNNCC 2 cut(s) 810, 880
BmrFI CCNGG 4 cut(s) 50, 323, 336, 876
BmrI ACTGGG 2 cut(s) 117, 660
BmsI GCATC 4 cut(s) 178, 244, 567, 897
BmuI ACTGGG 2 cut(s) 117, 660
BpmI CTGGAG 2 cut(s) 318, 474
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 876
BsaAI YACGTR 1 cut(s) 595
BsaBI GATNNNNATC 1 cut(s) 24
BsaHI GRCGYC 1 cut(s) 7
BsaJI CCNNGG 1 cut(s) 874
BsaWI WCCGGW 2 cut(s) 598, 834
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 617, 968
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 3 cut(s) 123, 568, 655
Bse21I CCTNAGG 1 cut(s) 12
Bse3DI GCAATG 2 cut(s) 67, 333
Bse8I GATNNNNATC 1 cut(s) 24
BseBI CCWGG 3 cut(s) 50, 323, 336
BseDI CCNNGG 1 cut(s) 874
BseGI GGATG 4 cut(s) 58, 349, 891, 977
BseJI GATNNNNATC 1 cut(s) 24
BseLI CCNNNNNNNGG 3 cut(s) 322, 617, 968
BseMI GCAATG 2 cut(s) 67, 333
BseMII CTCAG 2 cut(s) 961, 1061
BseNI ACTGG 3 cut(s) 123, 568, 655
BseRI GAGGAG 7 cut(s) 360, 369, 372, 375, 420, 423, 477
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 4 cut(s) 481, 569, 636, 990
BseYI CCCAGC 2 cut(s) 303, 528
BsgI GTGCAG 1 cut(s) 811
BshFI GGCC 6 cut(s) 183, 346, 427, 723, 750, 954
BshNI GGYRCC 1 cut(s) 808
BshTI ACCGGT 1 cut(s) 598
BsiHKAI GWGCWC 4 cut(s) 276, 606, 919, 948
BsiSI CCGG 4 cut(s) 599, 835, 866, 876
BslFI GGGAC 2 cut(s) 18, 864
BslI CCNNNNNNNGG 3 cut(s) 322, 617, 968
BsmAI GTCTC 2 cut(s) 117, 408
BsmBI CGTCTC 1 cut(s) 117
BsmFI GGGAC 2 cut(s) 18, 864
BsnI GGCC 6 cut(s) 183, 346, 427, 723, 750, 954
Bsp1286I GDGCHC 4 cut(s) 276, 606, 919, 948
Bsp143I GATC 6 cut(s) 19, 25, 160, 262, 461, 786
BspACI CCGC 3 cut(s) 17, 248, 627
BspANI GGCC 6 cut(s) 183, 346, 427, 723, 750, 954
BspCNI CTCAG 3 cut(s) 4, 960, 1060
BspHI TCATGA 2 cut(s) 163, 690
BspLI GGNNCC 2 cut(s) 810, 880
BspMAI CTGCAG 2 cut(s) 559, 1008
BspMI ACCTGC 1 cut(s) 870
BspPI GGATC 2 cut(s) 27, 469
BspT107I GGYRCC 1 cut(s) 808
BsrDI GCAATG 2 cut(s) 67, 333
BsrFI RCCGGY 1 cut(s) 598
BsrI ACTGG 3 cut(s) 123, 568, 655
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 1 cut(s) 874
BssMI GATC 6 cut(s) 19, 25, 160, 262, 461, 786
BssNAI GTATAC 1 cut(s) 569
BssNI GRCGYC 1 cut(s) 7
Bst1107I GTATAC 1 cut(s) 569
Bst2UI CCWGG 3 cut(s) 50, 323, 336
Bst4CI ACNGT 3 cut(s) 36, 615, 1027
BstACI GRCGYC 1 cut(s) 7
BstBAI YACGTR 1 cut(s) 595
BstC8I GCNNGC 1 cut(s) 908
BstDEI CTNAG 4 cut(s) 12, 845, 947, 1047
BstENI CCTNNNNNAGG 1 cut(s) 966
BstF5I GGATG 4 cut(s) 58, 349, 891, 977
BstKTI GATC 6 cut(s) 22, 28, 163, 265, 464, 789
BstMAI GTCTC 2 cut(s) 117, 408
BstMBI GATC 6 cut(s) 19, 25, 160, 262, 461, 786
BstMWI GCNNNNNNNGC 1 cut(s) 862
BstNI CCWGG 3 cut(s) 50, 323, 336
BstNSI RCATGY 1 cut(s) 57
BstSCI CCNGG 4 cut(s) 48, 321, 334, 874
BstSFI CTRYAG 3 cut(s) 421, 555, 1004
BstSLI GKGCMC 1 cut(s) 276
BstSNI TACGTA 1 cut(s) 595
BstV1I GCAGC 4 cut(s) 481, 569, 636, 990
BstZ17I GTATAC 1 cut(s) 569
Bsu36I CCTNAGG 1 cut(s) 12
BsuRI GGCC 6 cut(s) 183, 346, 427, 723, 750, 954
BtrI CACGTC 1 cut(s) 898
BtsCI GGATG 4 cut(s) 58, 349, 891, 977
BtsIMutI CAGTG 1 cut(s) 1032
BveI ACCTGC 1 cut(s) 870
Cac8I GCNNGC 1 cut(s) 908
CaiI CAGNNNCTG 2 cut(s) 563, 1003
CciI TCATGA 2 cut(s) 163, 690
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 1 cut(s) 878
Csp6I GTAC 4 cut(s) 481, 596, 636, 1023
CspAI ACCGGT 1 cut(s) 598
CviAII CATG 9 cut(s) 54, 164, 574, 607, 691, 784, 805, 813, 985
CviQI GTAC 4 cut(s) 481, 596, 636, 1023
DdeI CTNAG 4 cut(s) 12, 845, 947, 1047
DpnI GATC 6 cut(s) 21, 27, 162, 264, 463, 788
DpnII GATC 6 cut(s) 19, 25, 160, 262, 461, 786
DraI TTTAAA 2 cut(s) 414, 522
DraIII CACNNNGTG 1 cut(s) 271
EaeI YGGCCR 3 cut(s) 344, 721, 748
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 946
Eco105I TACGTA 1 cut(s) 595
Eco147I AGGCCT 2 cut(s) 427, 954
Eco24I GRGCYC 1 cut(s) 948
Eco47I GGWCC 1 cut(s) 878
Eco53kI GAGCTC 1 cut(s) 946
Eco57I CTGAAG 1 cut(s) 980
Eco81I CCTNAGG 1 cut(s) 12
EcoICRI GAGCTC 1 cut(s) 946
EcoNI CCTNNNNNAGG 1 cut(s) 966
EcoRII CCWGG 3 cut(s) 48, 321, 334
EcoT38I GRGCYC 1 cut(s) 948
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 9 cut(s) 57, 167, 577, 610, 694, 787, 808, 816, 988
FaqI GGGAC 2 cut(s) 18, 864
FatI CATG 9 cut(s) 53, 163, 573, 606, 690, 783, 804, 812, 984
FbaI TGATCA 3 cut(s) 160, 262, 786
FblI GTMKAC 1 cut(s) 568
Fnu4HI GCNGC 5 cut(s) 495, 558, 625, 628, 1004
FokI GGATG 4 cut(s) 45, 336, 878, 984
FriOI GRGCYC 1 cut(s) 948
Fsp4HI GCNGC 5 cut(s) 495, 558, 625, 628, 1004
GluI GCNGC 5 cut(s) 495, 558, 625, 628, 1004
GsaI CCCAGC 2 cut(s) 307, 532
GsuI CTGGAG 2 cut(s) 318, 474
HaeIII GGCC 6 cut(s) 183, 346, 427, 723, 750, 954
HapII CCGG 4 cut(s) 599, 835, 866, 876
Hin1I GRCGYC 1 cut(s) 7
Hin1II CATG 9 cut(s) 57, 167, 577, 610, 694, 787, 808, 816, 988
HindIII AAGCTT 1 cut(s) 854
HinfI GANTC 1 cut(s) 83
HpaII CCGG 4 cut(s) 599, 835, 866, 876
Hpy166II GTNNAC 3 cut(s) 274, 341, 569
Hpy188I TCNGA 5 cut(s) 88, 354, 950, 999, 1050
Hpy188III TCNNGA 8 cut(s) 11, 23, 133, 164, 173, 671, 691, 881
Hpy8I GTNNAC 3 cut(s) 274, 341, 569
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 3 cut(s) 384, 399, 972
HpyCH4III ACNGT 3 cut(s) 36, 615, 1027
HpyCH4IV ACGT 6 cut(s) 7, 194, 270, 594, 716, 897
HpyCH4V TGCA 8 cut(s) 274, 445, 557, 580, 731, 828, 888, 1006
HpyF10VI GCNNNNNNNGC 1 cut(s) 862
HpyF3I CTNAG 4 cut(s) 12, 845, 947, 1047
HpySE526I ACGT 6 cut(s) 7, 194, 270, 594, 716, 897
Hsp92I GRCGYC 1 cut(s) 7
Hsp92II CATG 9 cut(s) 57, 167, 577, 610, 694, 787, 808, 816, 988
Ksp22I TGATCA 3 cut(s) 160, 262, 786
Kzo9I GATC 6 cut(s) 19, 25, 160, 262, 461, 786
LmnI GCTCC 2 cut(s) 937, 988
Lsp1109I GCAGC 4 cut(s) 481, 569, 636, 990
LweI GCATC 4 cut(s) 178, 244, 567, 897
MaeII ACGT 6 cut(s) 7, 194, 270, 594, 716, 897
MaeIII GTNAC 1 cut(s) 1030
MalI GATC 6 cut(s) 21, 27, 162, 264, 463, 788
MboI GATC 6 cut(s) 19, 25, 160, 262, 461, 786
MboII GAAGA 1 cut(s) 790
MhlI GDGCHC 4 cut(s) 276, 606, 919, 948
MlsI TGGCCA 1 cut(s) 723
MluCI AATT 8 cut(s) 74, 205, 225, 448, 502, 514, 541, 550
MluNI TGGCCA 1 cut(s) 723
MmeI TCCRAC 4 cut(s) 69, 86, 377, 692
Mox20I TGGCCA 1 cut(s) 723
MscI TGGCCA 1 cut(s) 723
MseI TTAA 2 cut(s) 413, 521
MslI CAYNNNNRTG 2 cut(s) 695, 806
Msp20I TGGCCA 1 cut(s) 723
MspA1I CMGCKG 2 cut(s) 494, 627
MspI CCGG 4 cut(s) 599, 835, 866, 876
MspR9I CCNGG 4 cut(s) 50, 323, 336, 876
MvaI CCWGG 3 cut(s) 50, 323, 336
MwoI GCNNNNNNNGC 1 cut(s) 862
NciI CCSGG 1 cut(s) 876
NdeII GATC 6 cut(s) 19, 25, 160, 262, 461, 786
NlaIII CATG 9 cut(s) 57, 167, 577, 610, 694, 787, 808, 816, 988
NlaIV GGNNCC 2 cut(s) 810, 880
NmuCI GTSAC 1 cut(s) 1030
NspI RCATGY 1 cut(s) 57
PagI TCATGA 2 cut(s) 163, 690
PceI AGGCCT 2 cut(s) 427, 954
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PfeI GAWTC 1 cut(s) 83
PinAI ACCGGT 1 cut(s) 598
PkrI GCNGC 5 cut(s) 496, 559, 626, 629, 1005
Ppu21I YACGTR 1 cut(s) 595
Psp124BI GAGCTC 1 cut(s) 948
Psp6I CCWGG 3 cut(s) 48, 321, 334
PspFI CCCAGC 2 cut(s) 303, 528
PspGI CCWGG 3 cut(s) 48, 321, 334
PspN4I GGNNCC 2 cut(s) 810, 880
PspPI GGNCC 1 cut(s) 878
PstI CTGCAG 2 cut(s) 559, 1008
PstNI CAGNNNCTG 2 cut(s) 563, 1003
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 4 cut(s) 482, 597, 637, 1024
RsaNI GTAC 4 cut(s) 481, 596, 636, 1023
RseI CAYNNNNRTG 2 cut(s) 695, 806
SacI GAGCTC 1 cut(s) 948
SaqAI TTAA 2 cut(s) 413, 521
SatI GCNGC 5 cut(s) 495, 558, 625, 628, 1004
Sau3AI GATC 6 cut(s) 19, 25, 160, 262, 461, 786
Sau96I GGNCC 1 cut(s) 878
ScrFI CCNGG 4 cut(s) 50, 323, 336, 876
SduI GDGCHC 4 cut(s) 276, 606, 919, 948
SfaNI GCATC 4 cut(s) 178, 244, 567, 897
SfcI CTRYAG 3 cut(s) 421, 555, 1004
SinI GGWCC 1 cut(s) 878
SmiMI CAYNNNNRTG 2 cut(s) 695, 806
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
SnaBI TACGTA 1 cut(s) 595
Sse9I AATT 8 cut(s) 74, 205, 225, 448, 502, 514, 541, 550
SseBI AGGCCT 2 cut(s) 427, 954
SsiI CCGC 3 cut(s) 17, 248, 627
SstI GAGCTC 1 cut(s) 948
StuI AGGCCT 2 cut(s) 427, 954
StyD4I CCNGG 4 cut(s) 48, 321, 334, 874
TaaI ACNGT 3 cut(s) 36, 615, 1027
TaiI ACGT 6 cut(s) 10, 197, 273, 597, 719, 900
TaqI TCGA 2 cut(s) 38, 957
TasI AATT 8 cut(s) 74, 205, 225, 448, 502, 514, 541, 550
TauI GCSGC 1 cut(s) 630
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 2 cut(s) 413, 521
Tru9I TTAA 2 cut(s) 413, 521
TscAI CASTG 1 cut(s) 1032
TseFI GTSAC 1 cut(s) 1030
TseI GCWGC 4 cut(s) 494, 557, 624, 1003
Tsp45I GTSAC 1 cut(s) 1030
TspDTI ATGAA 1 cut(s) 790
TspRI CASTG 1 cut(s) 1032
VneI GTGCAC 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 878
XagI CCTNNNNNAGG 1 cut(s) 966
XceI RCATGY 1 cut(s) 57
XmiI GTMKAC 1 cut(s) 568
ZraI GACGTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.