Rroxscaffold_3G00269280

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000960
Physical Location & Seq
Reverse (-)
62186758 .. 62188349
1592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269280.1

Sequence Viewer

Length: 1296 bp
ATGGAGGATGATCATCCCACTCCAGATTACGAAGACCACCCCCACGATGATCAACCTGAAGCTCATGTGGAGGTACAAGTCGAACAATATCAATATCAACAAGTAGCGCTTGAAATTAGCGACAATAGTGGAATCTACGACCAAAACCAGCAGCAGCAGCAGCCACCTCAGTATCAGCCACAACCTCCACAAGGAGGCCAACAACAACAACAGTTCTTCCAGCAGCAGCAGCCGCCGCCGCGTCAACCATCTTATCAGCCGCCTGTACAATTCCCACCACAACAACCACCACCGCGGCCGATCCAATACTTGGCTCCACAGCCGCCTCGCCCTGTGCCATTCCCTCCTCAGAGCCCACAAAACCAAAACTACGCAAACCAGCCTCCTCCTCCAGGACCTCCACAACAGTTCCCACCGCAAAACCATCAGTTTCAAGCTCAACAGCCGCCTTATCAGCCACAGCCACCTCAACAGTTTCCACCACAGAATGCGACCCCTAACCAACAAAACAACCCTTTTGGACAACCACCTTCTCCGGCAAAATATCCTCCGCAACCACCTTCTCCGGTGAAATATCCTCCACAACCACCATCTCCGGCACAATATCCTCCTGCAGCAGGTTCGTCTCAGCCTCCTCAAGGCTACAATGACATTCCCCAACCTTCAAAGCAGCATCAGGAGCCGCCTCCGGTGCAAGTGGGCTATAATAAGCCTCAGCCACCCCAACAGTACCACCAGCAGCAACAATACCAACCACAACCACCACAGCAGAATCATTATGCGGCTGCTCCTTCTCCTGCTCAAGGAGTTCCGATGCAGGCCCCTTACGGACCGCAGGGAACGCCTATGACTATGAATATGATGAACCCGATTGGCACTGAGGCTTGGAAAACGGGGCTCTTTGATTGCATGGATGATCCTAATAATGCTCTCATAACGTTATTCGTACCATGCTTGACTTTCGGCCAGATAGCAGAAGTTGTGGATAATGGCACTACCTCATGTGGTACGAGCGCATTGCTGTACGGCTTGGTTGGAGCGTTCATCGGAGTGCCATTCATAATGTCATGCACGTATCGCACGAAGATTCGGAGCAAGTATGGGCTAGTTGAGACCCCTGCACCTGACTGGGTCACCCATTTGTTTTGCGAGCCATGTTCTCTTTGCCAAGAGTACAGAGAACTTAATTTGAGAGGGCTAGACCCTTCTATAGGATGGCAAGGAAATGTGGCAAGGGCCATGCAGCAGCAACAACCTAAGATGATGACACCTCCAGTGAACCAAATGATGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

431

Amino Acids

48.34

Weight (kDa)

5.16

Isoelectric Point (pI)

81.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 296 - 394 3e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 608
AccB7I CCANNNNNTGG 1 cut(s) 310
AccII CGCG 2 cut(s) 241, 295
AclI AACGTT 1 cut(s) 938
AclWI GGATC 2 cut(s) 295, 911
AcoI YGGCCR 2 cut(s) 296, 964
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 7 cut(s) 75, 267, 731, 948, 1009, 1025, 1175
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 8 cut(s) 191, 194, 310, 392, 617, 638, 803, 1211
AgsI TTSAA 3 cut(s) 113, 434, 666
AjnI CCWGG 1 cut(s) 391
AluBI AGCT 2 cut(s) 62, 437
AluI AGCT 2 cut(s) 62, 437
Alw26I GTCTC 2 cut(s) 630, 1106
AlwI GGATC 2 cut(s) 295, 911
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 5 cut(s) 196, 296, 819, 964, 1236
AspLEI GCGC 2 cut(s) 109, 1016
AspS9I GGNCC 4 cut(s) 395, 820, 830, 1236
AsuHPI GGTGA 2 cut(s) 580, 1126
AvaII GGWCC 2 cut(s) 395, 830
BaeI ACNNNNGTAYC 2 cut(s) 155, 188
BanII GRGCYC 2 cut(s) 356, 900
BbsI GAAGAC 1 cut(s) 39
BbvCI CCTCAGC 1 cut(s) 714
BccI CCATC 4 cut(s) 256, 432, 598, 1209
BceAI ACGGC 1 cut(s) 1042
BcgI CGANNNNNNTGC 4 cut(s) 603, 637, 1000, 1034
BciT130I CCWGG 1 cut(s) 393
BclI TGATCA 2 cut(s) 10, 49
BcoDI GTCTC 2 cut(s) 630, 1106
BfaI CTAG 2 cut(s) 1106, 1199
BfmI CTRYAG 2 cut(s) 612, 1209
BfoI RGCGCY 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 608
Bme1390I CCNGG 1 cut(s) 393
Bme18I GGWCC 2 cut(s) 395, 830
BmgT120I GGNCC 4 cut(s) 395, 820, 830, 1236
BmiI GGNNCC 3 cut(s) 315, 681, 822
BmrFI CCNGG 1 cut(s) 393
BmrI ACTGGG 1 cut(s) 1138
BmsI GCATC 2 cut(s) 682, 804
BmuI ACTGGG 1 cut(s) 1138
BpiI GAAGAC 1 cut(s) 39
BpmI CTGGAG 3 cut(s) 6, 375, 1257
Bpu10I CCTNAGC 1 cut(s) 714
BpuEI CTTGAG 2 cut(s) 621, 786
BsaAI YACGTR 1 cut(s) 1074
BsaBI GATNNNNATC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 1106
BsaJI CCNNGG 1 cut(s) 293
BsaWI WCCGGW 2 cut(s) 565, 688
Bsc4I CCNNNNNNNGG 8 cut(s) 191, 194, 310, 392, 617, 638, 803, 1211
Bse1I ACTGG 2 cut(s) 1133, 1274
Bse3DI GCAATG 1 cut(s) 1016
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 4 cut(s) 13, 13, 919, 1220
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 8 cut(s) 191, 194, 310, 392, 617, 638, 803, 1211
BseMI GCAATG 1 cut(s) 1016
BseMII CTCAG 5 cut(s) 182, 362, 641, 728, 870
BseNI ACTGG 2 cut(s) 1133, 1274
BseRI GAGGAG 4 cut(s) 336, 375, 378, 624
BseX3I CGGCCG 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 1104
Bsh1236I CGCG 2 cut(s) 241, 295
Bsh1285I CGRYCG 1 cut(s) 299
BshFI GGCC 5 cut(s) 198, 298, 821, 966, 1238
BsiEI CGRYCG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 536, 566, 596, 689
BslI CCNNNNNNNGG 8 cut(s) 191, 194, 310, 392, 617, 638, 803, 1211
BsmAI GTCTC 2 cut(s) 630, 1106
BsmBI CGTCTC 1 cut(s) 630
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 5 cut(s) 198, 298, 821, 966, 1238
Bso31I GGTCTC 1 cut(s) 1106
Bsp1286I GDGCHC 2 cut(s) 356, 900
Bsp1407I TGTACA 1 cut(s) 265
Bsp143I GATC 4 cut(s) 10, 49, 300, 916
BspANI GGCC 5 cut(s) 198, 298, 821, 966, 1238
BspCNI CTCAG 5 cut(s) 181, 361, 640, 727, 871
BspFNI CGCG 2 cut(s) 241, 295
BspLI GGNNCC 3 cut(s) 315, 681, 822
BspMAI CTGCAG 1 cut(s) 616
BspMI ACCTGC 1 cut(s) 608
BspPI GGATC 2 cut(s) 295, 911
BspTNI GGTCTC 1 cut(s) 1106
BsrDI GCAATG 1 cut(s) 1016
BsrGI TGTACA 1 cut(s) 265
BsrI ACTGG 2 cut(s) 1133, 1274
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 4 cut(s) 10, 49, 300, 916
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 4 cut(s) 213, 408, 474, 729
BstAUI TGTACA 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 1074
BstC8I GCNNGC 2 cut(s) 819, 1151
BstDEI CTNAG 6 cut(s) 168, 348, 627, 714, 879, 1257
BstDSI CCRYGG 1 cut(s) 293
BstEII GGTNACC 1 cut(s) 1132
BstENI CCTNNNNNAGG 6 cut(s) 189, 390, 615, 636, 801, 1209
BstF5I GGATG 4 cut(s) 13, 13, 919, 1220
BstFNI CGCG 2 cut(s) 241, 295
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 2 cut(s) 109, 1016
BstKTI GATC 4 cut(s) 13, 52, 303, 919
BstMAI GTCTC 2 cut(s) 630, 1106
BstMBI GATC 4 cut(s) 10, 49, 300, 916
BstMCI CGRYCG 1 cut(s) 299
BstNI CCWGG 1 cut(s) 393
BstPI GGTNACC 1 cut(s) 1132
BstSCI CCNGG 1 cut(s) 391
BstSFI CTRYAG 2 cut(s) 612, 1209
BstUI CGCG 2 cut(s) 241, 295
BstV2I GAAGAC 1 cut(s) 39
BstZI CGGCCG 1 cut(s) 296
BsuRI GGCC 5 cut(s) 198, 298, 821, 966, 1238
BtgI CCRYGG 1 cut(s) 293
BtsCI GGATG 4 cut(s) 13, 13, 919, 1220
BtsIMutI CAGTG 2 cut(s) 876, 1281
BveI ACCTGC 1 cut(s) 608
Cac8I GCNNGC 2 cut(s) 819, 1151
CfoI GCGC 2 cut(s) 109, 1016
Cfr13I GGNCC 4 cut(s) 395, 820, 830, 1236
Cfr42I CCGCGG 1 cut(s) 296
CpoI CGGWCCG 1 cut(s) 830
CseI GACGC 1 cut(s) 230
Csp6I GTAC 7 cut(s) 74, 266, 730, 947, 1008, 1024, 1174
CspI CGGWCCG 1 cut(s) 830
CviAII CATG 7 cut(s) 65, 910, 951, 1002, 1068, 1155, 1240
CviQI GTAC 7 cut(s) 74, 266, 730, 947, 1008, 1024, 1174
DdeI CTNAG 6 cut(s) 168, 348, 627, 714, 879, 1257
DpnI GATC 4 cut(s) 12, 51, 302, 918
DpnII GATC 4 cut(s) 10, 49, 300, 916
EaeI YGGCCR 2 cut(s) 296, 964
EagI CGGCCG 1 cut(s) 296
EclXI CGGCCG 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 356, 900
Eco31I GGTCTC 1 cut(s) 1106
Eco47I GGWCC 2 cut(s) 395, 830
Eco47III AGCGCT 1 cut(s) 108
Eco52I CGGCCG 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 1132
EcoNI CCTNNNNNAGG 6 cut(s) 189, 390, 615, 636, 801, 1209
EcoO109I RGGNCCY 2 cut(s) 395, 820
EcoO65I GGTNACC 1 cut(s) 1132
EcoRII CCWGG 1 cut(s) 391
EcoT38I GRGCYC 2 cut(s) 356, 900
Esp3I CGTCTC 1 cut(s) 630
FaeI CATG 7 cut(s) 68, 913, 954, 1005, 1071, 1158, 1243
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FatI CATG 7 cut(s) 64, 909, 950, 1001, 1067, 1154, 1239
FbaI TGATCA 2 cut(s) 10, 49
FokI GGATG 3 cut(s) 20, 926, 1227
FriOI GRGCYC 2 cut(s) 356, 900
FspBI CTAG 2 cut(s) 1106, 1199
GlaI GCGC 2 cut(s) 108, 1015
GsuI CTGGAG 3 cut(s) 6, 375, 1257
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 5 cut(s) 198, 298, 821, 966, 1238
HapII CCGG 4 cut(s) 536, 566, 596, 689
HgaI GACGC 1 cut(s) 230
HhaI GCGC 2 cut(s) 109, 1016
Hin1II CATG 7 cut(s) 68, 913, 954, 1005, 1071, 1158, 1243
Hin6I GCGC 2 cut(s) 107, 1014
HinP1I GCGC 2 cut(s) 107, 1014
HincII GTYRAC 1 cut(s) 245
HindII GTYRAC 1 cut(s) 245
HinfI GANTC 3 cut(s) 132, 772, 1087
HpaII CCGG 4 cut(s) 536, 566, 596, 689
HphI GGTGA 2 cut(s) 580, 1126
Hpy166II GTNNAC 2 cut(s) 245, 1279
Hpy188I TCNGA 4 cut(s) 351, 813, 1049, 1092
Hpy188III TCNNGA 2 cut(s) 23, 677
Hpy8I GTNNAC 2 cut(s) 245, 1279
HpyAV CCTTC 5 cut(s) 540, 570, 672, 801, 1215
HpyCH4III ACNGT 4 cut(s) 213, 408, 474, 729
HpyCH4IV ACGT 2 cut(s) 938, 1073
HpyCH4V TGCA 7 cut(s) 614, 694, 817, 909, 1071, 1121, 1243
HpyF3I CTNAG 6 cut(s) 168, 348, 627, 714, 879, 1257
HpySE526I ACGT 2 cut(s) 938, 1073
Hsp92II CATG 7 cut(s) 68, 913, 954, 1005, 1071, 1158, 1243
HspAI GCGC 2 cut(s) 107, 1014
Ksp22I TGATCA 2 cut(s) 10, 49
KspI CCGCGG 1 cut(s) 296
Kzo9I GATC 4 cut(s) 10, 49, 300, 916
LmnI GCTCC 5 cut(s) 319, 679, 793, 1037, 1092
LweI GCATC 2 cut(s) 682, 804
MaeI CTAG 2 cut(s) 1106, 1199
MaeII ACGT 2 cut(s) 938, 1073
MaeIII GTNAC 1 cut(s) 1132
MalI GATC 4 cut(s) 12, 51, 302, 918
MboI GATC 4 cut(s) 10, 49, 300, 916
MboII GAAGA 3 cut(s) 44, 208, 1096
MhlI GDGCHC 2 cut(s) 356, 900
MluCI AATT 3 cut(s) 114, 269, 1186
MmeI TCCRAC 1 cut(s) 1015
MseI TTAA 1 cut(s) 1185
MslI CAYNNNNRTG 1 cut(s) 1049
MspA1I CMGCKG 1 cut(s) 295
MspI CCGG 4 cut(s) 536, 566, 596, 689
MspR9I CCNGG 1 cut(s) 393
Mva1269I GAATGC 1 cut(s) 493
MvaI CCWGG 1 cut(s) 393
MvnI CGCG 2 cut(s) 241, 295
NdeII GATC 4 cut(s) 10, 49, 300, 916
NlaIII CATG 7 cut(s) 68, 913, 954, 1005, 1071, 1158, 1243
NlaIV GGNNCC 3 cut(s) 315, 681, 822
NmuCI GTSAC 1 cut(s) 1132
PcsI WCGNNNNNNNCGW 1 cut(s) 1079
PctI GAATGC 1 cut(s) 493
PfeI GAWTC 3 cut(s) 132, 772, 1087
PflFI GACNNNGTC 1 cut(s) 1130
PflMI CCANNNNNTGG 1 cut(s) 310
PfoI TCCNGGA 1 cut(s) 391
Ppu21I YACGTR 1 cut(s) 1074
PpuMI RGGWCCY 1 cut(s) 395
Psp1406I AACGTT 1 cut(s) 938
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 391
PspEI GGTNACC 1 cut(s) 1132
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 3 cut(s) 315, 681, 822
PspPI GGNCC 4 cut(s) 395, 820, 830, 1236
PspPPI RGGWCCY 1 cut(s) 395
PstI CTGCAG 1 cut(s) 616
PsyI GACNNNGTC 1 cut(s) 1130
RsaI GTAC 7 cut(s) 75, 267, 731, 948, 1009, 1025, 1175
RsaNI GTAC 7 cut(s) 74, 266, 730, 947, 1008, 1024, 1174
RseI CAYNNNNRTG 1 cut(s) 1049
Rsr2I CGGWCCG 1 cut(s) 830
RsrII CGGWCCG 1 cut(s) 830
SacII CCGCGG 1 cut(s) 296
SaqAI TTAA 1 cut(s) 1185
Sau3AI GATC 4 cut(s) 10, 49, 300, 916
Sau96I GGNCC 4 cut(s) 395, 820, 830, 1236
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 2 cut(s) 356, 900
SfaNI GCATC 2 cut(s) 682, 804
SfcI CTRYAG 2 cut(s) 612, 1209
Sfr303I CCGCGG 1 cut(s) 296
SgrBI CCGCGG 1 cut(s) 296
SinI GGWCC 2 cut(s) 395, 830
SmiMI CAYNNNNRTG 1 cut(s) 1049
SmlI CTYRAG 2 cut(s) 636, 801
SmoI CTYRAG 2 cut(s) 636, 801
Sse9I AATT 3 cut(s) 114, 269, 1186
SspMI CTAG 2 cut(s) 1106, 1199
StyD4I CCNGG 1 cut(s) 391
TaaI ACNGT 4 cut(s) 213, 408, 474, 729
TaiI ACGT 2 cut(s) 941, 1076
TaqI TCGA 1 cut(s) 81
TasI AATT 3 cut(s) 114, 269, 1186
TatI WGTACW 2 cut(s) 265, 1173
TauI GCSGC 9 cut(s) 235, 238, 241, 262, 298, 325, 448, 685, 785
TfiI GAWTC 3 cut(s) 132, 772, 1087
Tru1I TTAA 1 cut(s) 1185
Tru9I TTAA 1 cut(s) 1185
TscAI CASTG 2 cut(s) 883, 1281
TseFI GTSAC 1 cut(s) 1132
Tsp45I GTSAC 1 cut(s) 1132
TspDTI ATGAA 4 cut(s) 869, 878, 1033, 1048
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 2 cut(s) 883, 1281
Tth111I GACNNNGTC 1 cut(s) 1130
Van91I CCANNNNNTGG 1 cut(s) 310
VpaK11BI GGWCC 2 cut(s) 395, 830
XagI CCTNNNNNAGG 6 cut(s) 189, 390, 615, 636, 801, 1209
XspI CTAG 2 cut(s) 1106, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.