pycom14g18540

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000960
Physical Location & Seq
Forward (+)
20154081 .. 20155754
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g18540.2

Sequence Viewer

Length: 1197 bp
ATGGGAAATCCAGAAGGAGGGCCGTCTGCGTACGACGGTGATCATGATCTCTACCAACCTCAAGTGGAGGTGGATTATGAGTTACAAGCTGATCAACAATCTCAATACCAACAAGTGTCTGAAACCACCAGACACGACCACAACCAACAAGATCAATACCAAGAGCCACCGCCACAGCCACCCAATAATGCACCTCAAAATAATTATGACCCCCAAGCACAACCACAAGGAGTCTTTCAGCAACTACCGCCATCTCAGCCTCAGCCAGCAGGGTTCCCACCACCACAAGGTCCTCCACAACAAAACCCCTACCAGACTCCAACGCCTCAGCCAGCACAGTTCCCTCCTCAAAACCCACAACAATTCCCACCACAAAACCCACAATTCCAAAATCAACAACAAGAAGCAAACTATCAGCAGCCTAGGCCTGCACAGTTTCCACCCCAGCCCCAGAGTAATATGCAAACCAATCCTAACCGTAATCCCATGTATGCAAATGTGCCTAACCAACCCGGGGCGGTCTACCCGCCTCAAGGACCACAACCAATGGGCGTGGGGAACCAACAAGCTCCAGCTCAGTTTCCTCCCCAATCTCCAGCGAATTATAATCAGCAATATCAGAAGCCGGCAGTGCAATTTTCCACACAGAATCAACAATACCAACAACAGCAGCCTAATAATGGTTATGCTGTTCAAGGTGTTCCAATGCAGCAGAGTCCTTACCAGCAGGGACCTCTTAATCACGTCAACCTGCAGAATGCTGGAACTGAGCAATGGAGTTCTGAGTTATTTGATTGCATGGATGATCCAATGAATGCTCTCACAACGGCTTTCGTTCCATGCTTGACGTTTGGACAGATAGCAGAGGTTATAGATAACGGCACATCCTCATGTGCGACCAGCGGACTGTTCTACGGATTGATTGCATCTTTCATTGGGATACCATTCATAATGTCATGCACGTATCGCACAAAGCTGCGCAGCAAATACAACCTAATCGAGTCCCCCGCACCCGATTGGGTCACCCATTTATTTTGCGAACCCTGCGCTCTTTGCCAAGAGTACCGGGAACTTCAAGTCAGAGGGATTGACCCTTCAATCGGATGGATAGGAAATGTGCAAAGGAATCCCAGCTTGCAGCAGCCGAATGTTAATATGATGATGCCTCCAATGAACCAAAATATGAATCAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

399

Amino Acids

44.67

Weight (kDa)

4.41

Isoelectric Point (pI)

72.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
Acc16I TGCGCA 1 cut(s) 980
Acc36I ACCTGC 1 cut(s) 759
AccI GTMKAC 1 cut(s) 522
AciI CCGC 6 cut(s) 170, 248, 518, 527, 903, 1008
AclWI GGATC 1 cut(s) 800
AfaI GTAC 2 cut(s) 32, 1064
AfiI CCNNNNNNNGG 6 cut(s) 17, 287, 514, 533, 680, 1100
AgsI TTSAA 3 cut(s) 695, 1076, 1098
AjiI CACGTC 1 cut(s) 745
AluBI AGCT 5 cut(s) 89, 569, 575, 976, 1134
AluI AGCT 5 cut(s) 89, 569, 575, 976, 1134
AlwI GGATC 1 cut(s) 800
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 2 cut(s) 20, 425
ApeKI GCWGC 7 cut(s) 418, 670, 709, 976, 981, 1138, 1141
AspA2I CCTAGG 1 cut(s) 422
AspLEI GCGC 2 cut(s) 981, 1049
AspS9I GGNCC 4 cut(s) 20, 290, 536, 731
AsuC2I CCSGG 3 cut(s) 513, 514, 1067
AsuHPI GGTGA 2 cut(s) 50, 1015
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 3 cut(s) 290, 536, 731
AvrII CCTAGG 1 cut(s) 422
BbvCI CCTCAGC 2 cut(s) 261, 327
BbvI GCAGC 7 cut(s) 430, 682, 721, 963, 993, 1150, 1153
BccI CCATC 2 cut(s) 259, 1098
BceAI ACGGC 3 cut(s) 7, 843, 895
BciVI GTATCC 1 cut(s) 933
BclI TGATCA 2 cut(s) 40, 91
BcnI CCSGG 3 cut(s) 513, 514, 1067
BfaI CTAG 1 cut(s) 423
BfmI CTRYAG 1 cut(s) 752
BfuAI ACCTGC 1 cut(s) 759
BfuI GTATCC 1 cut(s) 933
BisI GCNGC 7 cut(s) 419, 671, 710, 977, 982, 1139, 1142
BlnI CCTAGG 1 cut(s) 422
BlsI GCNGC 7 cut(s) 420, 672, 711, 978, 983, 1140, 1143
Bme1390I CCNGG 3 cut(s) 513, 514, 1067
Bme18I GGWCC 3 cut(s) 290, 536, 731
BmeT110I CYCGRG 1 cut(s) 512
BmgBI CACGTC 1 cut(s) 745
BmgT120I GGNCC 4 cut(s) 20, 290, 536, 731
BmiI GGNNCC 3 cut(s) 275, 560, 732
BmrFI CCNGG 3 cut(s) 513, 514, 1067
BmsI GCATC 2 cut(s) 935, 1152
BpmI CTGGAG 2 cut(s) 555, 579
Bpu10I CCTNAGC 2 cut(s) 261, 327
BpuEI CTTGAG 2 cut(s) 45, 516
BpuMI CCSGG 3 cut(s) 513, 514, 1067
BsaAI YACGTR 1 cut(s) 963
BsaBI GATNNNNATC 1 cut(s) 45
BsaJI CCNNGG 3 cut(s) 422, 512, 513
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 287, 514, 533, 680, 1100
Bse118I RCCGGY 1 cut(s) 625
Bse3DI GCAATG 1 cut(s) 779
Bse8I GATNNNNATC 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 422, 512, 513
BseGI GGATG 3 cut(s) 808, 884, 1109
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 6 cut(s) 17, 287, 514, 533, 680, 1100
BseMI GCAATG 1 cut(s) 779
BseMII CTCAG 6 cut(s) 269, 275, 341, 590, 759, 774
BseRI GAGGAG 1 cut(s) 336
BseXI GCAGC 7 cut(s) 430, 682, 721, 963, 993, 1150, 1153
BseYI CCCAGC 2 cut(s) 444, 1130
BsgI GTGCAG 1 cut(s) 414
BshFI GGCC 2 cut(s) 22, 427
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 3 cut(s) 513, 626, 1066
BsiWI CGTACG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 744, 988
BslI CCNNNNNNNGG 6 cut(s) 17, 287, 514, 533, 680, 1100
BsmFI GGGAC 2 cut(s) 744, 988
BsmI GAATGC 2 cut(s) 763, 820
BsnI GGCC 2 cut(s) 22, 427
BsoBI CYCGRG 1 cut(s) 512
Bsp143I GATC 5 cut(s) 40, 46, 91, 151, 805
BspACI CCGC 6 cut(s) 170, 248, 518, 527, 903, 1008
BspANI GGCC 2 cut(s) 22, 427
BspCNI CTCAG 6 cut(s) 268, 274, 340, 589, 760, 775
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 3 cut(s) 275, 560, 732
BspMAI CTGCAG 1 cut(s) 756
BspMI ACCTGC 1 cut(s) 759
BspPI GGATC 1 cut(s) 800
BsrDI GCAATG 1 cut(s) 779
BsrFI RCCGGY 1 cut(s) 625
BssAI RCCGGY 1 cut(s) 625
BssECI CCNNGG 3 cut(s) 422, 512, 513
BssMI GATC 5 cut(s) 40, 46, 91, 151, 805
BssT1I CCWWGG 1 cut(s) 422
Bst4CI ACNGT 5 cut(s) 38, 339, 435, 479, 909
BstBAI YACGTR 1 cut(s) 963
BstC8I GCNNGC 5 cut(s) 267, 333, 429, 627, 1136
BstDEI CTNAG 6 cut(s) 255, 261, 327, 576, 768, 783
BstEII GGTNACC 1 cut(s) 1021
BstF5I GGATG 3 cut(s) 808, 884, 1109
BstHHI GCGC 2 cut(s) 981, 1049
BstKTI GATC 5 cut(s) 43, 49, 94, 154, 808
BstMBI GATC 5 cut(s) 40, 46, 91, 151, 805
BstMWI GCNNNNNNNGC 7 cut(s) 247, 256, 424, 631, 966, 1044, 1053
BstPI GGTNACC 1 cut(s) 1021
BstSCI CCNGG 3 cut(s) 511, 512, 1065
BstSFI CTRYAG 1 cut(s) 752
BstV1I GCAGC 7 cut(s) 430, 682, 721, 963, 993, 1150, 1153
BsuI GTATCC 1 cut(s) 933
BsuRI GGCC 2 cut(s) 22, 427
BtrI CACGTC 1 cut(s) 745
BtsCI GGATG 3 cut(s) 808, 884, 1109
BtsI GCAGTG 1 cut(s) 636
BtsIMutI CAGTG 1 cut(s) 636
BveI ACCTGC 1 cut(s) 759
Cac8I GCNNGC 5 cut(s) 267, 333, 429, 627, 1136
CciI TCATGA 1 cut(s) 43
CfoI GCGC 2 cut(s) 981, 1049
Cfr10I RCCGGY 1 cut(s) 625
Cfr13I GGNCC 4 cut(s) 20, 290, 536, 731
Cfr9I CCCGGG 1 cut(s) 512
Csp6I GTAC 2 cut(s) 31, 1063
CspCI CAANNNNNGTGG 2 cut(s) 534, 569
CviAII CATG 6 cut(s) 44, 487, 799, 840, 891, 957
CviQI GTAC 2 cut(s) 31, 1063
DdeI CTNAG 6 cut(s) 255, 261, 327, 576, 768, 783
DpnI GATC 5 cut(s) 42, 48, 93, 153, 807
DpnII GATC 5 cut(s) 40, 46, 91, 151, 805
Eco130I CCWWGG 1 cut(s) 422
Eco147I AGGCCT 1 cut(s) 427
Eco47I GGWCC 3 cut(s) 290, 536, 731
Eco88I CYCGRG 1 cut(s) 512
Eco91I GGTNACC 1 cut(s) 1021
EcoO109I RGGNCCY 2 cut(s) 290, 731
EcoO65I GGTNACC 1 cut(s) 1021
EcoT14I CCWWGG 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 422
FaeI CATG 6 cut(s) 47, 490, 802, 843, 894, 960
FaqI GGGAC 2 cut(s) 744, 988
FatI CATG 6 cut(s) 43, 486, 798, 839, 890, 956
FauI CCCGC 2 cut(s) 534, 1015
FbaI TGATCA 2 cut(s) 40, 91
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 7 cut(s) 419, 671, 710, 977, 982, 1139, 1142
FokI GGATG 3 cut(s) 815, 871, 1116
Fsp4HI GCNGC 7 cut(s) 419, 671, 710, 977, 982, 1139, 1142
FspBI CTAG 1 cut(s) 423
FspI TGCGCA 1 cut(s) 980
GlaI GCGC 2 cut(s) 980, 1048
GluI GCNGC 7 cut(s) 419, 671, 710, 977, 982, 1139, 1142
GsaI CCCAGC 2 cut(s) 448, 1134
GsuI CTGGAG 2 cut(s) 555, 579
HaeIII GGCC 2 cut(s) 22, 427
HapII CCGG 3 cut(s) 513, 626, 1066
HhaI GCGC 2 cut(s) 981, 1049
Hin1II CATG 6 cut(s) 47, 490, 802, 843, 894, 960
Hin6I GCGC 2 cut(s) 979, 1047
HinP1I GCGC 2 cut(s) 979, 1047
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HinfI GANTC 7 cut(s) 231, 316, 649, 715, 1001, 1126, 1186
HpaII CCGG 3 cut(s) 513, 626, 1066
HphI GGTGA 2 cut(s) 50, 1015
Hpy166II GTNNAC 2 cut(s) 523, 748
Hpy188I TCNGA 5 cut(s) 121, 621, 784, 1082, 1103
Hpy188III TCNNGA 2 cut(s) 11, 44
Hpy8I GTNNAC 2 cut(s) 523, 748
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 8, 1104
HpyCH4III ACNGT 5 cut(s) 38, 339, 435, 479, 909
HpyCH4IV ACGT 3 cut(s) 744, 848, 962
HpyF10VI GCNNNNNNNGC 7 cut(s) 247, 256, 424, 631, 966, 1044, 1053
HpyF3I CTNAG 6 cut(s) 255, 261, 327, 576, 768, 783
HpySE526I ACGT 3 cut(s) 744, 848, 962
Hsp92II CATG 6 cut(s) 47, 490, 802, 843, 894, 960
HspAI GCGC 2 cut(s) 979, 1047
KroI GCCGGC 1 cut(s) 625
KroNI GCCGGC 1 cut(s) 627
Ksp22I TGATCA 2 cut(s) 40, 91
Kzo9I GATC 5 cut(s) 40, 46, 91, 151, 805
LmnI GCTCC 1 cut(s) 574
Lsp1109I GCAGC 7 cut(s) 430, 682, 721, 963, 993, 1150, 1153
LweI GCATC 2 cut(s) 935, 1152
MaeI CTAG 1 cut(s) 423
MaeII ACGT 3 cut(s) 744, 848, 962
MaeIII GTNAC 2 cut(s) 81, 1021
MalI GATC 5 cut(s) 42, 48, 93, 153, 807
MboI GATC 5 cut(s) 40, 46, 91, 151, 805
MluCI AATT 5 cut(s) 202, 362, 383, 601, 635
MlyI GAGTC 4 cut(s) 240, 310, 724, 1010
MmeI TCCRAC 1 cut(s) 344
MroNI GCCGGC 1 cut(s) 625
MseI TTAA 2 cut(s) 738, 1152
MslI CAYNNNNRTG 1 cut(s) 889
MspA1I CMGCKG 1 cut(s) 903
MspI CCGG 3 cut(s) 513, 626, 1066
MspR9I CCNGG 3 cut(s) 513, 514, 1067
Mva1269I GAATGC 2 cut(s) 763, 820
MwoI GCNNNNNNNGC 7 cut(s) 247, 256, 424, 631, 966, 1044, 1053
NaeI GCCGGC 1 cut(s) 627
NciI CCSGG 3 cut(s) 513, 514, 1067
NdeII GATC 5 cut(s) 40, 46, 91, 151, 805
NgoMIV GCCGGC 1 cut(s) 625
NlaIII CATG 6 cut(s) 47, 490, 802, 843, 894, 960
NlaIV GGNNCC 3 cut(s) 275, 560, 732
NmuCI GTSAC 1 cut(s) 1021
NsbI TGCGCA 1 cut(s) 980
PagI TCATGA 1 cut(s) 43
PceI AGGCCT 1 cut(s) 427
PctI GAATGC 2 cut(s) 763, 820
PdiI GCCGGC 1 cut(s) 627
PfeI GAWTC 3 cut(s) 649, 1126, 1186
Pfl23II CGTACG 1 cut(s) 30
PkrI GCNGC 7 cut(s) 420, 672, 711, 978, 983, 1140, 1143
PleI GAGTC 4 cut(s) 239, 310, 723, 1009
PpsI GAGTC 4 cut(s) 239, 310, 723, 1009
Ppu21I YACGTR 1 cut(s) 963
PpuMI RGGWCCY 2 cut(s) 290, 731
PsiI TTATAA 1 cut(s) 606
Psp5II RGGWCCY 2 cut(s) 290, 731
PspEI GGTNACC 1 cut(s) 1021
PspFI CCCAGC 2 cut(s) 444, 1130
PspLI CGTACG 1 cut(s) 30
PspN4I GGNNCC 3 cut(s) 275, 560, 732
PspPI GGNCC 4 cut(s) 20, 290, 536, 731
PspPPI RGGWCCY 2 cut(s) 290, 731
PstI CTGCAG 1 cut(s) 756
RsaI GTAC 2 cut(s) 32, 1064
RsaNI GTAC 2 cut(s) 31, 1063
RseI CAYNNNNRTG 1 cut(s) 889
SaqAI TTAA 2 cut(s) 738, 1152
SatI GCNGC 7 cut(s) 419, 671, 710, 977, 982, 1139, 1142
Sau3AI GATC 5 cut(s) 40, 46, 91, 151, 805
Sau96I GGNCC 4 cut(s) 20, 290, 536, 731
SchI GAGTC 4 cut(s) 240, 310, 724, 1010
ScrFI CCNGG 3 cut(s) 513, 514, 1067
SfaNI GCATC 2 cut(s) 935, 1152
SfcI CTRYAG 1 cut(s) 752
SinI GGWCC 3 cut(s) 290, 536, 731
SmaI CCCGGG 1 cut(s) 514
SmiMI CAYNNNNRTG 1 cut(s) 889
SmlI CTYRAG 2 cut(s) 60, 531
SmoI CTYRAG 2 cut(s) 60, 531
Sse9I AATT 5 cut(s) 202, 362, 383, 601, 635
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 6 cut(s) 170, 248, 518, 527, 903, 1008
SspMI CTAG 1 cut(s) 423
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 3 cut(s) 511, 512, 1065
StyI CCWWGG 1 cut(s) 422
TaaI ACNGT 5 cut(s) 38, 339, 435, 479, 909
TaiI ACGT 3 cut(s) 747, 851, 965
TaqI TCGA 1 cut(s) 999
TasI AATT 5 cut(s) 202, 362, 383, 601, 635
TfiI GAWTC 3 cut(s) 649, 1126, 1186
Tru1I TTAA 2 cut(s) 738, 1152
Tru9I TTAA 2 cut(s) 738, 1152
TscAI CASTG 1 cut(s) 636
TseFI GTSAC 1 cut(s) 1021
TseI GCWGC 7 cut(s) 418, 670, 709, 976, 981, 1138, 1141
Tsp45I GTSAC 1 cut(s) 1021
TspDTI ATGAA 4 cut(s) 827, 922, 937, 1187
TspGWI ACGGA 1 cut(s) 930
TspMI CCCGGG 1 cut(s) 512
TspRI CASTG 1 cut(s) 636
VpaK11BI GGWCC 3 cut(s) 290, 536, 731
XmaI CCCGGG 1 cut(s) 512
XmaJI CCTAGG 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 522
XspI CTAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.