RLG00000004939

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000960
Physical Location & Seq
Reverse (-)
63864239 .. 63865856
1618 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004939

Sequence Viewer

Length: 1044 bp
ATGGGACGTCCTGAGGCAGATCATGATCATCAAGCATCAAGTTCGAACCAGGCATTTCATCAAACAGATTGCGAACAACATCCCGAGAAACCTGAACACCAAGTTTTTCCTCCAAACCCCACTCATTGCCAGACCTATGTTTTAGGGCGTCCGTCTGGGGCAGAGAATGACGATCATCATCAACAACAAGAAGCCCAGCCTACAGAGCACAGTGCACCTCGAAACTCCTCCGCTGATTCCCAAGAAACTCAAGCAAATCAAACGCATCAACAAGAGCCGCAGTCACCTTATCATCACAATGTGCACTACTATTTCCCTACGCCCGACCCCCATCATAGTACTAATTTGACGTTGCATAATTTTCCTAACCAATATGCACCACGGGCCCAAGTACCCCAATTCCAAGCAATGCCTCCAGGTTTACGGCCATATCCGACCTTATATCCTCCTCCTCTCTATCATTCTCAACCTTTAAACAATTATAGTGGATCACAGCATGTACAACAAGTAGTATTGCACCCTCCTCCGGCTGCCTACAATTATCCTTCTAGTTCTAGAAACCCCCAGCCCTGTGTAAATTACAATAATTTTGCAGCAACTGGTATACCCATGATGCAAAATGTACAATACCTACCGCTGCTGATGCCTCAGTCCGCTACCGGCGAGGGTACTACTTGGAAAACAGGGCTCTGTGACTGCTTACTTGACCCCCCAAATGCTATAATGACAACGTTATGCCCATGTTGGACGTTCGGTCAGATTGCAGAGATTGTTGATAACGGTCAAACCTCCTGTAGTTGCAATGCTCTGATATACATGGTGATCTGGTGTTTCATCATGGTGCCATGCTTGTTGTCATGTACATACCGAAGAAAACTAAGGAACAAACTTGACCTGCCCGAGAGCCCCGGTCCCGACTGCATCATCCACTGCTTATGCGAGCTATGTGCTCTCTGCCAAGAACATAGGGAGCTTGAGCTCAGAGGCCTCGACCCTTCATTAGGTAAGCGAATTACTACGTTTTTTATTAGCCTTCATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

348

Amino Acids

39.12

Weight (kDa)

5.95

Isoelectric Point (pI)

58.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 226 - 324 6.2e-28 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 841
AccI GTMKAC 1 cut(s) 604
AciI CCGC 4 cut(s) 231, 278, 635, 654
AclI AACGTT 1 cut(s) 731
AclWI GGATC 1 cut(s) 496
AcoI YGGCCR 1 cut(s) 425
AcyI GRCGYC 2 cut(s) 7, 148
AdeI CACNNNGTG 1 cut(s) 301
AfaI GTAC 6 cut(s) 340, 393, 501, 624, 670, 862
AfiI CCNNNNNNNGG 2 cut(s) 526, 1001
AhdI GACNNNNNGTC 1 cut(s) 753
AjnI CCWGG 2 cut(s) 48, 415
AluBI AGCT 3 cut(s) 943, 973, 979
AluI AGCT 3 cut(s) 943, 973, 979
Alw21I GWGCWC 5 cut(s) 210, 217, 306, 952, 981
Alw44I GTGCAC 2 cut(s) 213, 302
AlwI GGATC 1 cut(s) 496
AlwNI CAGNNNCTG 1 cut(s) 599
Ama87I CYCGRG 2 cut(s) 83, 899
AoxI GGCC 3 cut(s) 384, 425, 985
ApaI GGGCCC 1 cut(s) 388
ApaLI GTGCAC 2 cut(s) 213, 302
ApeKI GCWGC 3 cut(s) 530, 593, 637
AspS9I GGNCC 3 cut(s) 384, 385, 911
AsuC2I CCSGG 1 cut(s) 909
AsuHPI GGTGA 2 cut(s) 276, 832
AsuII TTCGAA 1 cut(s) 44
AvaI CYCGRG 2 cut(s) 83, 899
AvaII GGWCC 1 cut(s) 911
AxyI CCTNAGG 1 cut(s) 12
BaeGI GKGCMC 3 cut(s) 217, 306, 388
BanI GGYRCC 1 cut(s) 841
BanII GRGCYC 4 cut(s) 388, 690, 908, 981
Bbv12I GWGCWC 5 cut(s) 210, 217, 306, 952, 981
BbvI GCAGC 3 cut(s) 517, 605, 624
BccI CCATC 1 cut(s) 339
BceAI ACGGC 1 cut(s) 440
BcgI CGANNNNNNTGC 4 cut(s) 24, 58, 929, 963
BciT130I CCWGG 2 cut(s) 50, 417
BclI TGATCA 1 cut(s) 25
BcnI CCSGG 1 cut(s) 909
BfaI CTAG 2 cut(s) 549, 555
BfmI CTRYAG 2 cut(s) 201, 793
BfuAI ACCTGC 1 cut(s) 903
BisI GCNGC 4 cut(s) 278, 531, 594, 638
BlsI GCNGC 4 cut(s) 279, 532, 595, 639
BmcAI AGTACT 1 cut(s) 340
Bme1390I CCNGG 3 cut(s) 50, 417, 909
Bme18I GGWCC 1 cut(s) 911
BmeRI GACNNNNNGTC 1 cut(s) 753
BmeT110I CYCGRG 2 cut(s) 83, 899
BmgT120I GGNCC 3 cut(s) 384, 385, 911
BmiI GGNNCC 3 cut(s) 386, 843, 913
BmrFI CCNGG 3 cut(s) 50, 417, 909
BmsI GCATC 5 cut(s) 44, 274, 603, 633, 930
BpmI CTGGAG 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 44
BpuEI CTTGAG 2 cut(s) 234, 995
BpuMI CCSGG 1 cut(s) 909
BsaBI GATNNNNATC 2 cut(s) 24, 177
BsaHI GRCGYC 2 cut(s) 7, 148
BsaJI CCNNGG 2 cut(s) 380, 907
Bsc4I CCNNNNNNNGG 2 cut(s) 526, 1001
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 1 cut(s) 604
Bse21I CCTNAGG 1 cut(s) 12
Bse3DI GCAATG 3 cut(s) 124, 414, 808
Bse8I GATNNNNATC 2 cut(s) 24, 177
BseBI CCWGG 2 cut(s) 50, 417
BseDI CCNNGG 2 cut(s) 380, 907
BseGI GGATG 2 cut(s) 79, 924
BseJI GATNNNNATC 2 cut(s) 24, 177
BseLI CCNNNNNNNGG 2 cut(s) 526, 1001
BseMI GCAATG 3 cut(s) 124, 414, 808
BseMII CTCAG 2 cut(s) 662, 994
BseNI ACTGG 1 cut(s) 604
BseRI GAGGAG 4 cut(s) 217, 438, 441, 513
BseSI GKGCMC 3 cut(s) 217, 306, 388
BseXI GCAGC 3 cut(s) 517, 605, 624
BseYI CCCAGC 2 cut(s) 195, 564
BshFI GGCC 3 cut(s) 386, 427, 987
BshNI GGYRCC 1 cut(s) 841
BsiHKAI GWGCWC 5 cut(s) 210, 217, 306, 952, 981
BsiHKCI CYCGRG 2 cut(s) 83, 899
BsiSI CCGG 3 cut(s) 527, 660, 909
BslFI GGGAC 2 cut(s) 18, 897
BslI CCNNNNNNNGG 2 cut(s) 526, 1001
BsmFI GGGAC 2 cut(s) 18, 897
BsnI GGCC 3 cut(s) 386, 427, 987
BsoBI CYCGRG 2 cut(s) 83, 899
Bsp119I TTCGAA 1 cut(s) 44
Bsp120I GGGCCC 1 cut(s) 384
Bsp1286I GDGCHC 8 cut(s) 210, 217, 306, 388, 690, 908, 952, 981
Bsp1407I TGTACA 3 cut(s) 499, 622, 860
Bsp143I GATC 5 cut(s) 19, 25, 172, 488, 822
BspACI CCGC 4 cut(s) 231, 278, 635, 654
BspANI GGCC 3 cut(s) 386, 427, 987
BspCNI CTCAG 3 cut(s) 4, 661, 993
BspHI TCATGA 1 cut(s) 22
BspLI GGNNCC 3 cut(s) 386, 843, 913
BspMI ACCTGC 1 cut(s) 903
BspPI GGATC 1 cut(s) 496
BspT104I TTCGAA 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 841
BsrDI GCAATG 3 cut(s) 124, 414, 808
BsrFI RCCGGY 1 cut(s) 659
BsrGI TGTACA 3 cut(s) 499, 622, 860
BsrI ACTGG 1 cut(s) 604
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 2 cut(s) 380, 907
BssMI GATC 5 cut(s) 19, 25, 172, 488, 822
BssNAI GTATAC 1 cut(s) 605
BssNI GRCGYC 2 cut(s) 7, 148
Bst1107I GTATAC 1 cut(s) 605
Bst2UI CCWGG 2 cut(s) 50, 417
Bst4CI ACNGT 2 cut(s) 212, 782
BstACI GRCGYC 2 cut(s) 7, 148
BstAUI TGTACA 3 cut(s) 499, 622, 860
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 941
BstDEI CTNAG 4 cut(s) 12, 648, 878, 980
BstDSI CCRYGG 1 cut(s) 380
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 2 cut(s) 79, 924
BstKTI GATC 5 cut(s) 22, 28, 175, 491, 825
BstMBI GATC 5 cut(s) 19, 25, 172, 488, 822
BstMWI GCNNNNNNNGC 3 cut(s) 205, 383, 643
BstNI CCWGG 2 cut(s) 50, 417
BstNSI RCATGY 1 cut(s) 500
BstSCI CCNGG 3 cut(s) 48, 415, 907
BstSFI CTRYAG 2 cut(s) 201, 793
BstSLI GKGCMC 3 cut(s) 217, 306, 388
BstV1I GCAGC 3 cut(s) 517, 605, 624
BstZ17I GTATAC 1 cut(s) 605
Bsu36I CCTNAGG 1 cut(s) 12
BsuRI GGCC 3 cut(s) 386, 427, 987
BtgI CCRYGG 1 cut(s) 380
BtsCI GGATG 2 cut(s) 79, 924
BtsI GCAGTG 1 cut(s) 928
BtsIMutI CAGTG 2 cut(s) 217, 928
BveI ACCTGC 1 cut(s) 903
Cac8I GCNNGC 1 cut(s) 941
CaiI CAGNNNCTG 1 cut(s) 599
CciI TCATGA 1 cut(s) 22
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 3 cut(s) 384, 385, 911
CseI GACGC 1 cut(s) 137
Csp6I GTAC 6 cut(s) 339, 392, 500, 623, 669, 861
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviAII CATG 8 cut(s) 23, 497, 610, 741, 817, 838, 846, 858
CviQI GTAC 6 cut(s) 339, 392, 500, 623, 669, 861
DdeI CTNAG 4 cut(s) 12, 648, 878, 980
DpnI GATC 5 cut(s) 21, 27, 174, 490, 824
DpnII GATC 5 cut(s) 19, 25, 172, 488, 822
DraI TTTAAA 1 cut(s) 474
DraIII CACNNNGTG 1 cut(s) 301
DriI GACNNNNNGTC 1 cut(s) 753
EaeI YGGCCR 1 cut(s) 425
Eam1105I GACNNNNNGTC 1 cut(s) 753
Ecl136II GAGCTC 1 cut(s) 979
Eco147I AGGCCT 1 cut(s) 987
Eco24I GRGCYC 4 cut(s) 388, 690, 908, 981
Eco47I GGWCC 1 cut(s) 911
Eco53kI GAGCTC 1 cut(s) 979
Eco81I CCTNAGG 1 cut(s) 12
Eco88I CYCGRG 2 cut(s) 83, 899
EcoICRI GAGCTC 1 cut(s) 979
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRII CCWGG 2 cut(s) 48, 415
EcoT38I GRGCYC 4 cut(s) 388, 690, 908, 981
FaeI CATG 8 cut(s) 26, 500, 613, 744, 820, 841, 849, 861
FaqI GGGAC 2 cut(s) 18, 897
FatI CATG 8 cut(s) 22, 496, 609, 740, 816, 837, 845, 857
FbaI TGATCA 1 cut(s) 25
FblI GTMKAC 1 cut(s) 604
Fnu4HI GCNGC 4 cut(s) 278, 531, 594, 638
FokI GGATG 2 cut(s) 66, 911
FriOI GRGCYC 4 cut(s) 388, 690, 908, 981
Fsp4HI GCNGC 4 cut(s) 278, 531, 594, 638
FspBI CTAG 2 cut(s) 549, 555
GluI GCNGC 4 cut(s) 278, 531, 594, 638
GsaI CCCAGC 2 cut(s) 199, 568
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 3 cut(s) 386, 427, 987
HapII CCGG 3 cut(s) 527, 660, 909
HgaI GACGC 1 cut(s) 137
Hin1I GRCGYC 2 cut(s) 7, 148
Hin1II CATG 8 cut(s) 26, 500, 613, 744, 820, 841, 849, 861
HinfI GANTC 1 cut(s) 236
HpaII CCGG 3 cut(s) 527, 660, 909
HphI GGTGA 2 cut(s) 276, 832
Hpy166II GTNNAC 4 cut(s) 215, 304, 422, 605
Hpy188I TCNGA 4 cut(s) 435, 759, 810, 983
Hpy188III TCNNGA 5 cut(s) 11, 23, 83, 555, 914
Hpy8I GTNNAC 4 cut(s) 215, 304, 422, 605
HpyAV CCTTC 3 cut(s) 555, 1005, 1043
HpyCH4III ACNGT 2 cut(s) 212, 782
HpyCH4IV ACGT 5 cut(s) 7, 350, 731, 749, 1019
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 383, 643
HpyF3I CTNAG 4 cut(s) 12, 648, 878, 980
HpySE526I ACGT 5 cut(s) 7, 350, 731, 749, 1019
Hsp92I GRCGYC 2 cut(s) 7, 148
Hsp92II CATG 8 cut(s) 26, 500, 613, 744, 820, 841, 849, 861
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 5 cut(s) 19, 25, 172, 488, 822
LmnI GCTCC 1 cut(s) 970
Lsp1109I GCAGC 3 cut(s) 517, 605, 624
LweI GCATC 5 cut(s) 44, 274, 603, 633, 930
MaeI CTAG 2 cut(s) 549, 555
MaeII ACGT 5 cut(s) 7, 350, 731, 749, 1019
MaeIII GTNAC 2 cut(s) 282, 692
MalI GATC 5 cut(s) 21, 27, 174, 490, 824
MboI GATC 5 cut(s) 19, 25, 172, 488, 822
MboII GAAGA 1 cut(s) 882
MhlI GDGCHC 8 cut(s) 210, 217, 306, 388, 690, 908, 952, 981
MluCI AATT 8 cut(s) 343, 358, 398, 478, 538, 577, 586, 1011
MmeI TCCRAC 2 cut(s) 458, 725
MseI TTAA 1 cut(s) 473
MslI CAYNNNNRTG 2 cut(s) 297, 839
MspA1I CMGCKG 2 cut(s) 233, 637
MspI CCGG 3 cut(s) 527, 660, 909
MspR9I CCNGG 3 cut(s) 50, 417, 909
MvaI CCWGG 2 cut(s) 50, 417
MwoI GCNNNNNNNGC 3 cut(s) 205, 383, 643
NciI CCSGG 1 cut(s) 909
NdeII GATC 5 cut(s) 19, 25, 172, 488, 822
NlaIII CATG 8 cut(s) 26, 500, 613, 744, 820, 841, 849, 861
NlaIV GGNNCC 3 cut(s) 386, 843, 913
NmuCI GTSAC 2 cut(s) 282, 692
NspI RCATGY 1 cut(s) 500
NspV TTCGAA 1 cut(s) 44
PagI TCATGA 1 cut(s) 22
PceI AGGCCT 1 cut(s) 987
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 4 cut(s) 279, 532, 595, 639
Psp124BI GAGCTC 1 cut(s) 981
Psp1406I AACGTT 1 cut(s) 731
Psp6I CCWGG 2 cut(s) 48, 415
PspFI CCCAGC 2 cut(s) 195, 564
PspGI CCWGG 2 cut(s) 48, 415
PspN4I GGNNCC 3 cut(s) 386, 843, 913
PspOMI GGGCCC 1 cut(s) 384
PspPI GGNCC 3 cut(s) 384, 385, 911
PstNI CAGNNNCTG 1 cut(s) 599
RsaI GTAC 6 cut(s) 340, 393, 501, 624, 670, 862
RsaNI GTAC 6 cut(s) 339, 392, 500, 623, 669, 861
RseI CAYNNNNRTG 2 cut(s) 297, 839
SacI GAGCTC 1 cut(s) 981
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 4 cut(s) 278, 531, 594, 638
Sau3AI GATC 5 cut(s) 19, 25, 172, 488, 822
Sau96I GGNCC 3 cut(s) 384, 385, 911
ScaI AGTACT 1 cut(s) 340
ScrFI CCNGG 3 cut(s) 50, 417, 909
SduI GDGCHC 8 cut(s) 210, 217, 306, 388, 690, 908, 952, 981
SfaNI GCATC 5 cut(s) 44, 274, 603, 633, 930
SfcI CTRYAG 2 cut(s) 201, 793
SfuI TTCGAA 1 cut(s) 44
SinI GGWCC 1 cut(s) 911
SmiMI CAYNNNNRTG 2 cut(s) 297, 839
SmlI CTYRAG 2 cut(s) 249, 974
SmoI CTYRAG 2 cut(s) 249, 974
Sse9I AATT 8 cut(s) 343, 358, 398, 478, 538, 577, 586, 1011
SseBI AGGCCT 1 cut(s) 987
SsiI CCGC 4 cut(s) 231, 278, 635, 654
SspMI CTAG 2 cut(s) 549, 555
SstI GAGCTC 1 cut(s) 981
StuI AGGCCT 1 cut(s) 987
StyD4I CCNGG 3 cut(s) 48, 415, 907
TaaI ACNGT 2 cut(s) 212, 782
TaiI ACGT 5 cut(s) 10, 353, 734, 752, 1022
TaqI TCGA 3 cut(s) 44, 220, 990
TaqII GACCGA 1 cut(s) 743
TasI AATT 8 cut(s) 343, 358, 398, 478, 538, 577, 586, 1011
TatI WGTACW 4 cut(s) 338, 499, 622, 860
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TscAI CASTG 2 cut(s) 217, 935
TseFI GTSAC 2 cut(s) 282, 692
TseI GCWGC 3 cut(s) 530, 593, 637
Tsp45I GTSAC 2 cut(s) 282, 692
TspDTI ATGAA 4 cut(s) 47, 823, 987, 1025
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 2 cut(s) 217, 935
VneI GTGCAC 2 cut(s) 213, 302
VpaK11BI GGWCC 1 cut(s) 911
XagI CCTNNNNNAGG 1 cut(s) 999
XbaI TCTAGA 1 cut(s) 554
XceI RCATGY 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 604
XspI CTAG 2 cut(s) 549, 555
ZraI GACGTC 1 cut(s) 8
ZrmI AGTACT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.