MD16G1222500.v1.1

Cell number regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000960
Physical Location & Seq
Forward (+)
22330136 .. 22330975
840 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1222500.v1.1.491

Sequence Viewer

Length: 372 bp
ATGATATTTACCCTCACCCCCAATAGTGAAGATGGTTACATAACATGCTTTCTCCCATGCATCACCTTCGGCCAAATTGCTGAAACTGTGGATGAAGGACGAAGCTCTTGCGTAAACCAAAGCACAGTGTACGGGTTGTTGTTCACGATTCAGTGCCATTGGTTCTACTCGTGGCTATATAGAGAGAAATTACGCCAAAATTTTGGTTTGCCGGAGGAGCCTTGTTCTGATTGCTGTGTTCATTTCTGGTGCTACGCATGTGCTCTTTGCCAAGAACATGCTGAGCTCAAACATAGAGGGTTCGACCCCTCCAAAGGTTGGACTGGGCCACCAACCGTTGCTCCACAGGTGCCTCCTTCCATGTTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

124

Amino Acids

14.06

Weight (kDa)

5.44

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 8 - 95 4e-26 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 349
AccB7I CCANNNNNTGG 1 cut(s) 318
AcoI YGGCCR 1 cut(s) 70
AcsI RAATTY 1 cut(s) 199
AfaI GTAC 1 cut(s) 131
AfiI CCNNNNNNNGG 2 cut(s) 314, 318
AgsI TTSAA 1 cut(s) 367
AluBI AGCT 2 cut(s) 105, 286
AluI AGCT 2 cut(s) 105, 286
Alw21I GWGCWC 2 cut(s) 265, 288
AoxI GGCC 2 cut(s) 70, 326
ApoI RAATTY 1 cut(s) 199
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 7, 55
BanI GGYRCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 288
BauI CACGAG 1 cut(s) 169
Bbv12I GWGCWC 2 cut(s) 265, 288
BccI CCATC 1 cut(s) 26
BcgI CGANNNNNNTGC 4 cut(s) 49, 83, 90, 124
BlpI GCTNAGC 1 cut(s) 282
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 2 cut(s) 219, 351
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 69
BmuI ACTGGG 1 cut(s) 333
Bpu1102I GCTNAGC 1 cut(s) 282
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 318
Bse1I ACTGG 1 cut(s) 328
BseGI GGATG 1 cut(s) 97
BseLI CCNNNNNNNGG 2 cut(s) 314, 318
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 1 cut(s) 230
BshFI GGCC 2 cut(s) 72, 328
BshNI GGYRCC 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 265, 288
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 314, 318
BsnI GGCC 2 cut(s) 72, 328
Bsp1286I GDGCHC 2 cut(s) 265, 288
Bsp1720I GCTNAGC 1 cut(s) 282
BspANI GGCC 2 cut(s) 72, 328
BspCNI CTCAG 1 cut(s) 274
BspLI GGNNCC 2 cut(s) 219, 351
BspT107I GGYRCC 1 cut(s) 349
BsrI ACTGG 1 cut(s) 328
BssSI CACGAG 1 cut(s) 169
Bst2BI CACGAG 1 cut(s) 169
Bst4CI ACNGT 3 cut(s) 88, 127, 337
BstDEI CTNAG 1 cut(s) 282
BstF5I GGATG 1 cut(s) 97
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNSI RCATGY 3 cut(s) 48, 261, 281
BstXI CCANNNNNNTGG 1 cut(s) 203
BsuRI GGCC 2 cut(s) 72, 328
BtsCI GGATG 1 cut(s) 97
BtsIMutI CAGTG 2 cut(s) 132, 158
Cfr13I GGNCC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 130
CviAII CATG 5 cut(s) 45, 57, 258, 278, 361
CviJI RGCY 6 cut(s) 72, 105, 175, 220, 286, 328
CviKI_1 RGCY 6 cut(s) 72, 105, 175, 220, 286, 328
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 1 cut(s) 282
EaeI YGGCCR 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 286
EcoT22I ATGCAT 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 288
FaeI CATG 5 cut(s) 48, 60, 261, 281, 364
FaiI YATR 9 cut(s) 41, 46, 58, 178, 180, 259, 279, 294, 362
FatI CATG 5 cut(s) 44, 56, 257, 277, 360
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 1 cut(s) 288
HaeIII GGCC 2 cut(s) 72, 328
HapII CCGG 1 cut(s) 212
Hin1II CATG 5 cut(s) 48, 60, 261, 281, 364
HinfI GANTC 1 cut(s) 148
HpaII CCGG 1 cut(s) 212
HphI GGTGA 2 cut(s) 7, 55
Hpy166II GTNNAC 3 cut(s) 115, 130, 144
Hpy188I TCNGA 1 cut(s) 229
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 3 cut(s) 115, 130, 144
HpyAV CCTTC 3 cut(s) 76, 89, 366
HpyCH4III ACNGT 3 cut(s) 88, 127, 337
HpyCH4V TGCA 1 cut(s) 60
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 1 cut(s) 282
Hsp92II CATG 5 cut(s) 48, 60, 261, 281, 364
LmnI GCTCC 2 cut(s) 217, 346
LpnPI CCDG 4 cut(s) 225, 232, 309, 332
LweI GCATC 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 35
MboII GAAGA 1 cut(s) 41
MhlI GDGCHC 2 cut(s) 265, 288
MluCI AATT 3 cut(s) 75, 188, 199
MmeI TCCRAC 1 cut(s) 299
MnlI CCTC 5 cut(s) 23, 208, 290, 319, 363
Mph1103I ATGCAT 1 cut(s) 62
MspI CCGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 217
NlaIII CATG 5 cut(s) 48, 60, 261, 281, 364
NlaIV GGNNCC 2 cut(s) 219, 351
NsiI ATGCAT 1 cut(s) 62
NspI RCATGY 3 cut(s) 48, 261, 281
PfeI GAWTC 1 cut(s) 148
PflMI CCANNNNNTGG 1 cut(s) 318
Psp124BI GAGCTC 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 219, 351
PspPI GGNCC 1 cut(s) 326
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
SacI GAGCTC 1 cut(s) 288
Sau96I GGNCC 1 cut(s) 326
SduI GDGCHC 2 cut(s) 265, 288
SetI ASST 5 cut(s) 68, 107, 288, 319, 351
SfaNI GCATC 1 cut(s) 69
Sse9I AATT 3 cut(s) 75, 188, 199
SstI GAGCTC 1 cut(s) 288
TaaI ACNGT 3 cut(s) 88, 127, 337
TaqI TCGA 1 cut(s) 303
TasI AATT 3 cut(s) 75, 188, 199
TfiI GAWTC 1 cut(s) 148
TscAI CASTG 2 cut(s) 132, 158
TspDTI ATGAA 2 cut(s) 108, 230
TspRI CASTG 2 cut(s) 132, 158
Van91I CCANNNNNTGG 1 cut(s) 318
XapI RAATTY 1 cut(s) 199
XceI RCATGY 3 cut(s) 48, 261, 281
Zsp2I ATGCAT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.