Rh7CG072000

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000960
Physical Location & Seq
Forward (+)
5114719 .. 5116455
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG072000.1

Sequence Viewer

Length: 1317 bp
ATGGAGGATGATCATCACACTCCAGATTACGAAGACCACCCCCACGATCATCAACCTGAAGCTCATGTGGAGGTACAAGTCGAACAATATCAATATCAACAAGTAGCGCTTGAAATTAGCGACAATAGTGTAAACTACGACCAAAACCAGCAGCAGCAGCAGCAACAACAGCAGCAGCCACCTCAGTATCAGCCACAACCTCCACCACAAGGAGGCCAACAACAACAACAGTTCTTCCAGCAGCAGCAGCCGCCGCCGCAGCGTCAACCATCTTATCAGCCGCCTGTACAATTCCCACCACAACAACCACCACCGCGGCCGATCCAATACTTGGCTCCACAGCCGCCTCGCCCTGTGCCATTCCCTCCTCAGAGCCCACAAAACCAAAAGTACGCAAACCAGCCTCCTCCTCCAGGACCTCCACAACAGTTCCCACCGCAAAACCCTCAGTTTCAAGCTCAACAGCCGCCTTATCAGCCACAGCCACCTCAACAGTTTCCACCACAGAATGCGACCCCTAACCAACAAAACAACCCTTTTGGACAGCCACCTTCTCCGGCAAAATATCCTCCGCAACCACCTTCTCCGGTGAAATATCCTCCACAACCACCATCTCCGGTACAATATCCTCCTGCAGCAGGTTCGTCTCAGCCTCCTCAAGGCTACAATGACATTCCCCAACCTTCAAAGCAGCATCAAGCGCCGCCTCCGGTGCAAGTGGGCTATAATAAGCCTCAGCCACCCCAACAGTACCACCAGCAGCAACAATACCAACCACAACCACCACAGCAGAATCATTATGCGGCTGCTCCTTCTCCGGCTCAAGGAGTTCCGATGCAGGCCCCTTACGGGCCGCAGGGAACGCCTATGACTATGAATATGATGAACCCGATTGGCACTGAGGCTTGGAAAACCGGGCTCTTTGATTGCATGGATGATCCTAATAATGCTCTCATAACGTTATTCGTACCATGCTTGACTTTCGGCCAGATAGCAGAAGTTGTGGATAATGGCACTACCTCATGTGGTACGAGCGCATTGCTGTACGGCTTGGTTGGAGCGTTCATCGGAGTGCCATTCATAATGTCATGCACTTATCGCACGAAGATTCGGAGCAAGTATGGGCTAGTTGAGACCCCTGCACCTGACTGGGTCACCCATTTGTTTTGCGAGCCATGTTCTCTTTGCCAAGAGTACAGAGAACTTAATTTGAGAGGGCTAGACCCTTCTATAGGATGGCAAGGAAATGTGGCAAGGGCCATGCAGCAGCAACAACCTAAGATGATGACGCCTCCAGTGAACCAAATGATGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

438

Amino Acids

49.25

Weight (kDa)

5.63

Isoelectric Point (pI)

83.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 303 - 401 3.1e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 629
AccB7I CCANNNNNTGG 1 cut(s) 331
AccII CGCG 1 cut(s) 316
AclI AACGTT 1 cut(s) 959
AclWI GGATC 2 cut(s) 316, 932
AcoI YGGCCR 2 cut(s) 317, 985
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 1289
AfaI GTAC 9 cut(s) 75, 288, 392, 621, 752, 969, 1030, 1046, 1196
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 9 cut(s) 209, 212, 331, 413, 638, 659, 824, 849, 1232
AgsI TTSAA 3 cut(s) 113, 455, 687
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 2 cut(s) 62, 458
AluI AGCT 2 cut(s) 62, 458
Alw26I GTCTC 2 cut(s) 651, 1127
AlwI GGATC 2 cut(s) 316, 932
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 6 cut(s) 214, 317, 840, 851, 985, 1257
AspLEI GCGC 3 cut(s) 109, 703, 1037
AspS9I GGNCC 4 cut(s) 416, 841, 851, 1257
AsuC2I CCSGG 1 cut(s) 916
AsuHPI GGTGA 2 cut(s) 601, 1147
AvaII GGWCC 1 cut(s) 416
BaeI ACNNNNGTAYC 2 cut(s) 170, 203
BanII GRGCYC 2 cut(s) 377, 921
BbsI GAAGAC 1 cut(s) 39
BbvCI CCTCAGC 1 cut(s) 735
BccI CCATC 3 cut(s) 277, 619, 1230
BceAI ACGGC 1 cut(s) 1063
BcgI CGANNNNNNTGC 4 cut(s) 624, 658, 1021, 1055
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 1 cut(s) 10
BcnI CCSGG 1 cut(s) 916
BcoDI GTCTC 2 cut(s) 651, 1127
BfaI CTAG 2 cut(s) 1127, 1220
BfmI CTRYAG 2 cut(s) 633, 1230
BfoI RGCGCY 2 cut(s) 110, 704
BfuAI ACCTGC 1 cut(s) 629
Bme1390I CCNGG 2 cut(s) 414, 916
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 4 cut(s) 416, 841, 851, 1257
BmiI GGNNCC 2 cut(s) 336, 843
BmrFI CCNGG 2 cut(s) 414, 916
BmrI ACTGGG 1 cut(s) 1159
BmsI GCATC 2 cut(s) 703, 825
BmuI ACTGGG 1 cut(s) 1159
BpiI GAAGAC 1 cut(s) 39
BpmI CTGGAG 3 cut(s) 6, 396, 1278
Bpu10I CCTNAGC 1 cut(s) 735
BpuEI CTTGAG 2 cut(s) 642, 807
BpuMI CCSGG 1 cut(s) 916
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 1289
BsaI GGTCTC 1 cut(s) 1127
BsaJI CCNNGG 1 cut(s) 314
BsaWI WCCGGW 3 cut(s) 586, 616, 709
Bsc4I CCNNNNNNNGG 9 cut(s) 209, 212, 331, 413, 638, 659, 824, 849, 1232
Bse1I ACTGG 2 cut(s) 1154, 1295
Bse3DI GCAATG 1 cut(s) 1037
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 3 cut(s) 13, 940, 1241
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 9 cut(s) 209, 212, 331, 413, 638, 659, 824, 849, 1232
BseMI GCAATG 1 cut(s) 1037
BseMII CTCAG 6 cut(s) 197, 383, 461, 662, 749, 891
BseNI ACTGG 2 cut(s) 1154, 1295
BseRI GAGGAG 4 cut(s) 357, 396, 399, 645
BseX3I CGGCCG 1 cut(s) 317
BsgI GTGCAG 1 cut(s) 1125
Bsh1236I CGCG 1 cut(s) 316
Bsh1285I CGRYCG 1 cut(s) 320
BshFI GGCC 6 cut(s) 216, 319, 842, 853, 987, 1259
BsiEI CGRYCG 1 cut(s) 320
BsiSI CCGG 6 cut(s) 557, 587, 617, 710, 818, 915
BslI CCNNNNNNNGG 9 cut(s) 209, 212, 331, 413, 638, 659, 824, 849, 1232
BsmAI GTCTC 2 cut(s) 651, 1127
BsmBI CGTCTC 1 cut(s) 651
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 6 cut(s) 216, 319, 842, 853, 987, 1259
Bso31I GGTCTC 1 cut(s) 1127
Bsp1286I GDGCHC 2 cut(s) 377, 921
Bsp1407I TGTACA 1 cut(s) 286
Bsp143I GATC 4 cut(s) 10, 46, 321, 937
BspANI GGCC 6 cut(s) 216, 319, 842, 853, 987, 1259
BspCNI CTCAG 6 cut(s) 196, 382, 460, 661, 748, 892
BspFNI CGCG 1 cut(s) 316
BspLI GGNNCC 2 cut(s) 336, 843
BspMAI CTGCAG 1 cut(s) 637
BspMI ACCTGC 1 cut(s) 629
BspPI GGATC 2 cut(s) 316, 932
BspTNI GGTCTC 1 cut(s) 1127
BsrDI GCAATG 1 cut(s) 1037
BsrGI TGTACA 1 cut(s) 286
BsrI ACTGG 2 cut(s) 1154, 1295
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 4 cut(s) 10, 46, 321, 937
BssNI GRCGYC 1 cut(s) 1289
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 4 cut(s) 231, 429, 495, 750
BstACI GRCGYC 1 cut(s) 1289
BstAUI TGTACA 1 cut(s) 286
BstC8I GCNNGC 2 cut(s) 840, 1172
BstDEI CTNAG 7 cut(s) 183, 369, 447, 648, 735, 900, 1278
BstDSI CCRYGG 1 cut(s) 314
BstEII GGTNACC 1 cut(s) 1153
BstENI CCTNNNNNAGG 4 cut(s) 411, 636, 657, 1230
BstF5I GGATG 3 cut(s) 13, 940, 1241
BstFNI CGCG 1 cut(s) 316
BstH2I RGCGCY 2 cut(s) 110, 704
BstHHI GCGC 3 cut(s) 109, 703, 1037
BstKTI GATC 4 cut(s) 13, 49, 324, 940
BstMAI GTCTC 2 cut(s) 651, 1127
BstMBI GATC 4 cut(s) 10, 46, 321, 937
BstMCI CGRYCG 1 cut(s) 320
BstNI CCWGG 1 cut(s) 414
BstPI GGTNACC 1 cut(s) 1153
BstSCI CCNGG 2 cut(s) 412, 914
BstSFI CTRYAG 2 cut(s) 633, 1230
BstUI CGCG 1 cut(s) 316
BstV2I GAAGAC 1 cut(s) 39
BstZI CGGCCG 1 cut(s) 317
BsuRI GGCC 6 cut(s) 216, 319, 842, 853, 987, 1259
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 3 cut(s) 13, 940, 1241
BtsIMutI CAGTG 2 cut(s) 897, 1302
BveI ACCTGC 1 cut(s) 629
Cac8I GCNNGC 2 cut(s) 840, 1172
CfoI GCGC 3 cut(s) 109, 703, 1037
Cfr13I GGNCC 4 cut(s) 416, 841, 851, 1257
Cfr42I CCGCGG 1 cut(s) 317
CseI GACGC 2 cut(s) 251, 1297
Csp6I GTAC 9 cut(s) 74, 287, 391, 620, 751, 968, 1029, 1045, 1195
CviAII CATG 7 cut(s) 65, 931, 972, 1023, 1089, 1176, 1261
CviQI GTAC 9 cut(s) 74, 287, 391, 620, 751, 968, 1029, 1045, 1195
DdeI CTNAG 7 cut(s) 183, 369, 447, 648, 735, 900, 1278
DpnI GATC 4 cut(s) 12, 48, 323, 939
DpnII GATC 4 cut(s) 10, 46, 321, 937
EaeI YGGCCR 2 cut(s) 317, 985
EagI CGGCCG 1 cut(s) 317
EclXI CGGCCG 1 cut(s) 317
Eco24I GRGCYC 2 cut(s) 377, 921
Eco31I GGTCTC 1 cut(s) 1127
Eco47I GGWCC 1 cut(s) 416
Eco47III AGCGCT 1 cut(s) 108
Eco52I CGGCCG 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 1153
EcoNI CCTNNNNNAGG 4 cut(s) 411, 636, 657, 1230
EcoO109I RGGNCCY 2 cut(s) 416, 841
EcoO65I GGTNACC 1 cut(s) 1153
EcoRII CCWGG 1 cut(s) 412
EcoT38I GRGCYC 2 cut(s) 377, 921
Esp3I CGTCTC 1 cut(s) 651
FaeI CATG 7 cut(s) 68, 934, 975, 1026, 1092, 1179, 1264
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FatI CATG 7 cut(s) 64, 930, 971, 1022, 1088, 1175, 1260
FbaI TGATCA 1 cut(s) 10
FokI GGATG 3 cut(s) 20, 947, 1248
FriOI GRGCYC 2 cut(s) 377, 921
FspBI CTAG 2 cut(s) 1127, 1220
GlaI GCGC 3 cut(s) 108, 702, 1036
GsuI CTGGAG 3 cut(s) 6, 396, 1278
HaeII RGCGCY 2 cut(s) 110, 704
HaeIII GGCC 6 cut(s) 216, 319, 842, 853, 987, 1259
HapII CCGG 6 cut(s) 557, 587, 617, 710, 818, 915
HgaI GACGC 2 cut(s) 251, 1297
HhaI GCGC 3 cut(s) 109, 703, 1037
Hin1I GRCGYC 1 cut(s) 1289
Hin1II CATG 7 cut(s) 68, 934, 975, 1026, 1092, 1179, 1264
Hin6I GCGC 3 cut(s) 107, 701, 1035
HinP1I GCGC 3 cut(s) 107, 701, 1035
HincII GTYRAC 1 cut(s) 266
HindII GTYRAC 1 cut(s) 266
HinfI GANTC 2 cut(s) 793, 1108
HpaII CCGG 6 cut(s) 557, 587, 617, 710, 818, 915
HphI GGTGA 2 cut(s) 601, 1147
Hpy166II GTNNAC 3 cut(s) 133, 266, 1300
Hpy188I TCNGA 4 cut(s) 372, 834, 1070, 1113
Hpy188III TCNNGA 1 cut(s) 23
Hpy8I GTNNAC 3 cut(s) 133, 266, 1300
HpyAV CCTTC 5 cut(s) 561, 591, 693, 822, 1236
HpyCH4III ACNGT 4 cut(s) 231, 429, 495, 750
HpyCH4IV ACGT 1 cut(s) 959
HpyCH4V TGCA 7 cut(s) 635, 715, 838, 930, 1092, 1142, 1264
HpyF3I CTNAG 7 cut(s) 183, 369, 447, 648, 735, 900, 1278
HpySE526I ACGT 1 cut(s) 959
Hsp92I GRCGYC 1 cut(s) 1289
Hsp92II CATG 7 cut(s) 68, 934, 975, 1026, 1092, 1179, 1264
HspAI GCGC 3 cut(s) 107, 701, 1035
Ksp22I TGATCA 1 cut(s) 10
KspI CCGCGG 1 cut(s) 317
Kzo9I GATC 4 cut(s) 10, 46, 321, 937
LmnI GCTCC 4 cut(s) 340, 814, 1058, 1113
LweI GCATC 2 cut(s) 703, 825
MaeI CTAG 2 cut(s) 1127, 1220
MaeII ACGT 1 cut(s) 959
MaeIII GTNAC 1 cut(s) 1153
MalI GATC 4 cut(s) 12, 48, 323, 939
MboI GATC 4 cut(s) 10, 46, 321, 937
MboII GAAGA 3 cut(s) 44, 226, 1117
MhlI GDGCHC 2 cut(s) 377, 921
MluCI AATT 3 cut(s) 114, 290, 1207
MmeI TCCRAC 1 cut(s) 1036
MseI TTAA 1 cut(s) 1206
MslI CAYNNNNRTG 1 cut(s) 1070
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 6 cut(s) 557, 587, 617, 710, 818, 915
MspR9I CCNGG 2 cut(s) 414, 916
Mva1269I GAATGC 1 cut(s) 514
MvaI CCWGG 1 cut(s) 414
MvnI CGCG 1 cut(s) 316
NciI CCSGG 1 cut(s) 916
NdeII GATC 4 cut(s) 10, 46, 321, 937
NlaIII CATG 7 cut(s) 68, 934, 975, 1026, 1092, 1179, 1264
NlaIV GGNNCC 2 cut(s) 336, 843
NmuCI GTSAC 1 cut(s) 1153
PctI GAATGC 1 cut(s) 514
PfeI GAWTC 2 cut(s) 793, 1108
PflFI GACNNNGTC 1 cut(s) 1151
PflMI CCANNNNNTGG 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 412
PpuMI RGGWCCY 1 cut(s) 416
Psp1406I AACGTT 1 cut(s) 959
Psp5II RGGWCCY 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 412
PspEI GGTNACC 1 cut(s) 1153
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 336, 843
PspPI GGNCC 4 cut(s) 416, 841, 851, 1257
PspPPI RGGWCCY 1 cut(s) 416
PstI CTGCAG 1 cut(s) 637
PsyI GACNNNGTC 1 cut(s) 1151
RsaI GTAC 9 cut(s) 75, 288, 392, 621, 752, 969, 1030, 1046, 1196
RsaNI GTAC 9 cut(s) 74, 287, 391, 620, 751, 968, 1029, 1045, 1195
RseI CAYNNNNRTG 1 cut(s) 1070
SacII CCGCGG 1 cut(s) 317
SaqAI TTAA 1 cut(s) 1206
Sau3AI GATC 4 cut(s) 10, 46, 321, 937
Sau96I GGNCC 4 cut(s) 416, 841, 851, 1257
ScrFI CCNGG 2 cut(s) 414, 916
SduI GDGCHC 2 cut(s) 377, 921
SfaNI GCATC 2 cut(s) 703, 825
SfcI CTRYAG 2 cut(s) 633, 1230
Sfr303I CCGCGG 1 cut(s) 317
SgrBI CCGCGG 1 cut(s) 317
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 1070
SmlI CTYRAG 2 cut(s) 657, 822
SmoI CTYRAG 2 cut(s) 657, 822
Sse9I AATT 3 cut(s) 114, 290, 1207
SspMI CTAG 2 cut(s) 1127, 1220
StyD4I CCNGG 2 cut(s) 412, 914
TaaI ACNGT 4 cut(s) 231, 429, 495, 750
TaiI ACGT 1 cut(s) 962
TaqI TCGA 1 cut(s) 81
TasI AATT 3 cut(s) 114, 290, 1207
TatI WGTACW 2 cut(s) 286, 1194
TfiI GAWTC 2 cut(s) 793, 1108
Tru1I TTAA 1 cut(s) 1206
Tru9I TTAA 1 cut(s) 1206
TscAI CASTG 2 cut(s) 904, 1302
TseFI GTSAC 1 cut(s) 1153
Tsp45I GTSAC 1 cut(s) 1153
TspDTI ATGAA 4 cut(s) 890, 899, 1054, 1069
TspRI CASTG 2 cut(s) 904, 1302
Tth111I GACNNNGTC 1 cut(s) 1151
Van91I CCANNNNNTGG 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 416
XagI CCTNNNNNAGG 4 cut(s) 411, 636, 657, 1230
XspI CTAG 2 cut(s) 1127, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.