Rh7BG070500

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000960
Physical Location & Seq
Forward (+)
4762107 .. 4763720
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG070500.1

Sequence Viewer

Length: 1311 bp
ATGGAGGATGATCATCACACTCCAGATTATGAAGATCATCATACTCCAGATTACGAAGACCACCCCCACGATGATCAACCTGAAGCTCATGTGGAGGTACAAGTCGAACAATATCAATATCAACAAGTAGCGCTTGAAATTAGCGACAATAGTGGAAACTACGACCAAAACCAGCAGCAGCAACAACAACAACAGCAGCAGCCACCTCAGTATCAGCCACAACCTCCACCACAAGGAGGCCAACAACAACAACAGTTCTTCCAGCAGAAGCAGCCGCCGCCGCCGCGTCAACCATCTTATCAGCCGCCTGTACAATTCCCACCACAACAACCACCACCGCGGCCGATCCAATACTTGGCTCAACAGCCGCCTCGCCCTGTGCCATTCCCTCCTCAGAGCCCACAAAACCAAAACTACGCAAACCAGCCTCCTCCTCCAGGACCTCCACAACAGTTCCCACCACAAAAACCTCAGATTCAAGCTCAACAGCCGCCTTATCAGCCACAGCCACCTCAACAGTTTCCACCACAGAATGCGGCCCCTAACCAACAAAACAACCCTTTTGGACAACCACCTTCTCCGGTGAAATATCCTCCACAACCACCATCTCCGGCACAATATCCTCCTGCAGCAGGTTCGTCTCAGCCTCCTCAAGGCTACAATGACATTCCCCAACCTTCAAAGCAGCATCAGGCGCCGCCTCCGGTGCAAGTGGGCTATAATAAGCCTCAGCCACCCCAACAGTACCACCAGCAGCAACAATACCAACCACAACAACCACAGCAGAATCATTATGCGGCTGCTCCTTCTCCGGCTCAAGGAGTTCCGATGCAGGCCCCTTACGGCCCGCAGGGAACGCCTATGACTATGAATATGATGAACCCGATTGGCACTGAGGCTTGGAAAACCGGGCTCTTTGATTGCATGGATGATCCTAATAATGCTCTCATAACGTTATTCGTACCATGCTTGACTTTCGGTCAGATAGCAGAAGTTGTGGATAATGGCACTACCTCATGTGGTACGAGCGCATTGCTGTACGGCTTGGTTGGAGCGTTCATCGGAGTGCCATTCATAATGTCATGCACTTATCGCACGAAGATTCGGAGCAAGTATGGGCTAGTTGAGACCCCTGCACCTGACTGGGTGACCCATTTGTTTTGCGAGCCATGTTCTCTTTGCCAAGAGTACAGAGAACTTAATTTGAGAGGGCTAGACCCTTCTATAGGATGGCAAGGAAATGTGGCAAGGGCCATGCAGCAGCAACAACCTAAGATGATGACGCCTCCAGTGAACCAAATGATGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

436

Amino Acids

49.06

Weight (kDa)

5.26

Isoelectric Point (pI)

79.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 301 - 399 3.1e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 623
AccB1I GGYRCC 1 cut(s) 694
AccB7I CCANNNNNTGG 1 cut(s) 355
AccII CGCG 2 cut(s) 286, 340
AclI AACGTT 1 cut(s) 953
AclWI GGATC 2 cut(s) 340, 926
AcoI YGGCCR 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 102
AcyI GRCGYC 2 cut(s) 695, 1283
AfaI GTAC 7 cut(s) 99, 312, 746, 963, 1024, 1040, 1190
AfeI AGCGCT 1 cut(s) 132
AfiI CCNNNNNNNGG 8 cut(s) 233, 236, 355, 437, 632, 653, 818, 1226
AgsI TTSAA 3 cut(s) 137, 479, 681
AhdI GACNNNNNGTC 1 cut(s) 978
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 2 cut(s) 86, 482
AluI AGCT 2 cut(s) 86, 482
Alw26I GTCTC 2 cut(s) 645, 1121
AlwI GGATC 2 cut(s) 340, 926
Aor51HI AGCGCT 1 cut(s) 132
AoxI GGCC 6 cut(s) 238, 341, 537, 834, 844, 1251
AspLEI GCGC 3 cut(s) 133, 697, 1031
AspS9I GGNCC 5 cut(s) 440, 538, 835, 845, 1251
AsuC2I CCSGG 1 cut(s) 910
AsuHPI GGTGA 2 cut(s) 595, 1159
AvaII GGWCC 1 cut(s) 440
BaeI ACNNNNGTAYC 2 cut(s) 194, 227
BanI GGYRCC 1 cut(s) 694
BanII GRGCYC 2 cut(s) 401, 915
BbsI GAAGAC 1 cut(s) 63
BbvCI CCTCAGC 1 cut(s) 729
BccI CCATC 3 cut(s) 301, 613, 1224
BceAI ACGGC 2 cut(s) 859, 1057
BcgI CGANNNNNNTGC 4 cut(s) 618, 652, 1015, 1049
BciT130I CCWGG 1 cut(s) 438
BclI TGATCA 2 cut(s) 10, 73
BcnI CCSGG 1 cut(s) 910
BcoDI GTCTC 2 cut(s) 645, 1121
BfaI CTAG 2 cut(s) 1121, 1214
BfmI CTRYAG 2 cut(s) 627, 1224
BfoI RGCGCY 2 cut(s) 134, 698
BfuAI ACCTGC 1 cut(s) 623
Bme1390I CCNGG 2 cut(s) 438, 910
Bme18I GGWCC 1 cut(s) 440
BmeRI GACNNNNNGTC 1 cut(s) 978
BmgT120I GGNCC 5 cut(s) 440, 538, 835, 845, 1251
BmiI GGNNCC 3 cut(s) 540, 696, 837
BmrFI CCNGG 2 cut(s) 438, 910
BmrI ACTGGG 1 cut(s) 1153
BmsI GCATC 2 cut(s) 697, 819
BmuI ACTGGG 1 cut(s) 1153
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 4 cut(s) 6, 30, 420, 1272
Bpu10I CCTNAGC 1 cut(s) 729
BpuEI CTTGAG 2 cut(s) 636, 801
BpuMI CCSGG 1 cut(s) 910
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 2 cut(s) 695, 1283
BsaI GGTCTC 1 cut(s) 1121
BsaJI CCNNGG 1 cut(s) 338
BsaWI WCCGGW 2 cut(s) 580, 703
Bsc4I CCNNNNNNNGG 8 cut(s) 233, 236, 355, 437, 632, 653, 818, 1226
Bse1I ACTGG 2 cut(s) 1148, 1289
Bse3DI GCAATG 1 cut(s) 1031
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 3 cut(s) 13, 934, 1235
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 8 cut(s) 233, 236, 355, 437, 632, 653, 818, 1226
BseMI GCAATG 1 cut(s) 1031
BseMII CTCAG 6 cut(s) 221, 407, 485, 656, 743, 885
BseNI ACTGG 2 cut(s) 1148, 1289
BseRI GAGGAG 4 cut(s) 381, 420, 423, 639
BseX3I CGGCCG 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 1119
Bsh1236I CGCG 2 cut(s) 286, 340
Bsh1285I CGRYCG 1 cut(s) 344
BshFI GGCC 6 cut(s) 240, 343, 539, 836, 846, 1253
BshNI GGYRCC 1 cut(s) 694
BsiEI CGRYCG 1 cut(s) 344
BsiSI CCGG 5 cut(s) 581, 611, 704, 812, 909
BslI CCNNNNNNNGG 8 cut(s) 233, 236, 355, 437, 632, 653, 818, 1226
BsmAI GTCTC 2 cut(s) 645, 1121
BsmBI CGTCTC 1 cut(s) 645
BsmI GAATGC 1 cut(s) 538
BsnI GGCC 6 cut(s) 240, 343, 539, 836, 846, 1253
Bso31I GGTCTC 1 cut(s) 1121
Bsp1286I GDGCHC 2 cut(s) 401, 915
Bsp1407I TGTACA 1 cut(s) 310
Bsp143I GATC 5 cut(s) 10, 34, 73, 345, 931
BspANI GGCC 6 cut(s) 240, 343, 539, 836, 846, 1253
BspCNI CTCAG 6 cut(s) 220, 406, 484, 655, 742, 886
BspFNI CGCG 2 cut(s) 286, 340
BspLI GGNNCC 3 cut(s) 540, 696, 837
BspMAI CTGCAG 1 cut(s) 631
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 2 cut(s) 340, 926
BspT107I GGYRCC 1 cut(s) 694
BspTNI GGTCTC 1 cut(s) 1121
BsrDI GCAATG 1 cut(s) 1031
BsrGI TGTACA 1 cut(s) 310
BsrI ACTGG 2 cut(s) 1148, 1289
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 5 cut(s) 10, 34, 73, 345, 931
BssNI GRCGYC 2 cut(s) 695, 1283
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 4 cut(s) 255, 453, 519, 744
BstACI GRCGYC 2 cut(s) 695, 1283
BstAUI TGTACA 1 cut(s) 310
BstC8I GCNNGC 3 cut(s) 834, 848, 1166
BstDEI CTNAG 7 cut(s) 207, 393, 471, 642, 729, 894, 1272
BstDSI CCRYGG 1 cut(s) 338
BstEII GGTNACC 1 cut(s) 1147
BstENI CCTNNNNNAGG 4 cut(s) 435, 630, 651, 1224
BstF5I GGATG 3 cut(s) 13, 934, 1235
BstFNI CGCG 2 cut(s) 286, 340
BstH2I RGCGCY 2 cut(s) 134, 698
BstHHI GCGC 3 cut(s) 133, 697, 1031
BstKTI GATC 5 cut(s) 13, 37, 76, 348, 934
BstMAI GTCTC 2 cut(s) 645, 1121
BstMBI GATC 5 cut(s) 10, 34, 73, 345, 931
BstMCI CGRYCG 1 cut(s) 344
BstMWI GCNNNNNNNGC 9 cut(s) 271, 277, 280, 283, 499, 694, 706, 856, 1092
BstNI CCWGG 1 cut(s) 438
BstPI GGTNACC 1 cut(s) 1147
BstSCI CCNGG 2 cut(s) 436, 908
BstSFI CTRYAG 2 cut(s) 627, 1224
BstUI CGCG 2 cut(s) 286, 340
BstV2I GAAGAC 1 cut(s) 63
BstZI CGGCCG 1 cut(s) 341
BsuRI GGCC 6 cut(s) 240, 343, 539, 836, 846, 1253
BtgI CCRYGG 1 cut(s) 338
BtsCI GGATG 3 cut(s) 13, 934, 1235
BtsIMutI CAGTG 2 cut(s) 891, 1296
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 3 cut(s) 834, 848, 1166
CfoI GCGC 3 cut(s) 133, 697, 1031
Cfr13I GGNCC 5 cut(s) 440, 538, 835, 845, 1251
Cfr42I CCGCGG 1 cut(s) 341
CseI GACGC 2 cut(s) 275, 1291
Csp6I GTAC 7 cut(s) 98, 311, 745, 962, 1023, 1039, 1189
CviAII CATG 7 cut(s) 89, 925, 966, 1017, 1083, 1170, 1255
CviQI GTAC 7 cut(s) 98, 311, 745, 962, 1023, 1039, 1189
DdeI CTNAG 7 cut(s) 207, 393, 471, 642, 729, 894, 1272
DinI GGCGCC 1 cut(s) 696
DpnI GATC 5 cut(s) 12, 36, 75, 347, 933
DpnII GATC 5 cut(s) 10, 34, 73, 345, 931
DriI GACNNNNNGTC 1 cut(s) 978
EaeI YGGCCR 1 cut(s) 341
EagI CGGCCG 1 cut(s) 341
Eam1105I GACNNNNNGTC 1 cut(s) 978
EclXI CGGCCG 1 cut(s) 341
Eco24I GRGCYC 2 cut(s) 401, 915
Eco31I GGTCTC 1 cut(s) 1121
Eco47I GGWCC 1 cut(s) 440
Eco47III AGCGCT 1 cut(s) 132
Eco52I CGGCCG 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 102
Eco91I GGTNACC 1 cut(s) 1147
EcoNI CCTNNNNNAGG 4 cut(s) 435, 630, 651, 1224
EcoO109I RGGNCCY 2 cut(s) 440, 835
EcoO65I GGTNACC 1 cut(s) 1147
EcoRII CCWGG 1 cut(s) 436
EcoT38I GRGCYC 2 cut(s) 401, 915
EgeI GGCGCC 1 cut(s) 696
EheI GGCGCC 1 cut(s) 696
Esp3I CGTCTC 1 cut(s) 645
FaeI CATG 7 cut(s) 92, 928, 969, 1020, 1086, 1173, 1258
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FatI CATG 7 cut(s) 88, 924, 965, 1016, 1082, 1169, 1254
FauI CCCGC 1 cut(s) 855
FbaI TGATCA 2 cut(s) 10, 73
FokI GGATG 3 cut(s) 20, 941, 1242
FriOI GRGCYC 2 cut(s) 401, 915
FspBI CTAG 2 cut(s) 1121, 1214
GlaI GCGC 3 cut(s) 132, 696, 1030
GsuI CTGGAG 4 cut(s) 6, 30, 420, 1272
HaeII RGCGCY 2 cut(s) 134, 698
HaeIII GGCC 6 cut(s) 240, 343, 539, 836, 846, 1253
HapII CCGG 5 cut(s) 581, 611, 704, 812, 909
HgaI GACGC 2 cut(s) 275, 1291
HhaI GCGC 3 cut(s) 133, 697, 1031
Hin1I GRCGYC 2 cut(s) 695, 1283
Hin1II CATG 7 cut(s) 92, 928, 969, 1020, 1086, 1173, 1258
Hin6I GCGC 3 cut(s) 131, 695, 1029
HinP1I GCGC 3 cut(s) 131, 695, 1029
HincII GTYRAC 1 cut(s) 290
HindII GTYRAC 1 cut(s) 290
HinfI GANTC 3 cut(s) 475, 787, 1102
HpaII CCGG 5 cut(s) 581, 611, 704, 812, 909
HphI GGTGA 2 cut(s) 595, 1159
Hpy166II GTNNAC 2 cut(s) 290, 1294
Hpy188I TCNGA 6 cut(s) 396, 474, 828, 984, 1064, 1107
Hpy188III TCNNGA 2 cut(s) 23, 47
Hpy8I GTNNAC 2 cut(s) 290, 1294
HpyAV CCTTC 4 cut(s) 585, 687, 816, 1230
HpyCH4III ACNGT 4 cut(s) 255, 453, 519, 744
HpyCH4IV ACGT 1 cut(s) 953
HpyCH4V TGCA 7 cut(s) 629, 709, 832, 924, 1086, 1136, 1258
HpyF10VI GCNNNNNNNGC 9 cut(s) 271, 277, 280, 283, 499, 694, 706, 856, 1092
HpyF3I CTNAG 7 cut(s) 207, 393, 471, 642, 729, 894, 1272
HpySE526I ACGT 1 cut(s) 953
Hsp92I GRCGYC 2 cut(s) 695, 1283
Hsp92II CATG 7 cut(s) 92, 928, 969, 1020, 1086, 1173, 1258
HspAI GCGC 3 cut(s) 131, 695, 1029
KasI GGCGCC 1 cut(s) 694
Ksp22I TGATCA 2 cut(s) 10, 73
KspI CCGCGG 1 cut(s) 341
Kzo9I GATC 5 cut(s) 10, 34, 73, 345, 931
LmnI GCTCC 3 cut(s) 808, 1052, 1107
LweI GCATC 2 cut(s) 697, 819
MaeI CTAG 2 cut(s) 1121, 1214
MaeII ACGT 1 cut(s) 953
MaeIII GTNAC 1 cut(s) 1147
MalI GATC 5 cut(s) 12, 36, 75, 347, 933
MboI GATC 5 cut(s) 10, 34, 73, 345, 931
MboII GAAGA 4 cut(s) 44, 68, 250, 1111
MhlI GDGCHC 2 cut(s) 401, 915
MluCI AATT 3 cut(s) 138, 314, 1201
Mly113I GGCGCC 1 cut(s) 695
MmeI TCCRAC 1 cut(s) 1030
MseI TTAA 1 cut(s) 1200
MslI CAYNNNNRTG 1 cut(s) 1064
MspA1I CMGCKG 1 cut(s) 340
MspI CCGG 5 cut(s) 581, 611, 704, 812, 909
MspR9I CCNGG 2 cut(s) 438, 910
Mva1269I GAATGC 1 cut(s) 538
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 2 cut(s) 286, 340
MwoI GCNNNNNNNGC 9 cut(s) 271, 277, 280, 283, 499, 694, 706, 856, 1092
NarI GGCGCC 1 cut(s) 695
NciI CCSGG 1 cut(s) 910
NdeII GATC 5 cut(s) 10, 34, 73, 345, 931
NlaIII CATG 7 cut(s) 92, 928, 969, 1020, 1086, 1173, 1258
NlaIV GGNNCC 3 cut(s) 540, 696, 837
NmuCI GTSAC 1 cut(s) 1147
PctI GAATGC 1 cut(s) 538
PfeI GAWTC 3 cut(s) 475, 787, 1102
PflMI CCANNNNNTGG 1 cut(s) 355
PfoI TCCNGGA 1 cut(s) 436
PluTI GGCGCC 1 cut(s) 698
PpuMI RGGWCCY 1 cut(s) 440
Psp1406I AACGTT 1 cut(s) 953
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 436
PspEI GGTNACC 1 cut(s) 1147
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 3 cut(s) 540, 696, 837
PspPI GGNCC 5 cut(s) 440, 538, 835, 845, 1251
PspPPI RGGWCCY 1 cut(s) 440
PstI CTGCAG 1 cut(s) 631
RsaI GTAC 7 cut(s) 99, 312, 746, 963, 1024, 1040, 1190
RsaNI GTAC 7 cut(s) 98, 311, 745, 962, 1023, 1039, 1189
RseI CAYNNNNRTG 1 cut(s) 1064
SacII CCGCGG 1 cut(s) 341
SaqAI TTAA 1 cut(s) 1200
Sau3AI GATC 5 cut(s) 10, 34, 73, 345, 931
Sau96I GGNCC 5 cut(s) 440, 538, 835, 845, 1251
ScrFI CCNGG 2 cut(s) 438, 910
SduI GDGCHC 2 cut(s) 401, 915
SfaNI GCATC 2 cut(s) 697, 819
SfcI CTRYAG 2 cut(s) 627, 1224
SfoI GGCGCC 1 cut(s) 696
Sfr303I CCGCGG 1 cut(s) 341
SgrBI CCGCGG 1 cut(s) 341
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 1064
SmlI CTYRAG 2 cut(s) 651, 816
SmoI CTYRAG 2 cut(s) 651, 816
Sse9I AATT 3 cut(s) 138, 314, 1201
SspDI GGCGCC 1 cut(s) 694
SspMI CTAG 2 cut(s) 1121, 1214
StyD4I CCNGG 2 cut(s) 436, 908
TaaI ACNGT 4 cut(s) 255, 453, 519, 744
TaiI ACGT 1 cut(s) 956
TaqI TCGA 1 cut(s) 105
TaqII GACCGA 1 cut(s) 968
TasI AATT 3 cut(s) 138, 314, 1201
TatI WGTACW 2 cut(s) 310, 1188
TfiI GAWTC 3 cut(s) 475, 787, 1102
Tru1I TTAA 1 cut(s) 1200
Tru9I TTAA 1 cut(s) 1200
TscAI CASTG 2 cut(s) 898, 1296
TseFI GTSAC 1 cut(s) 1147
Tsp45I GTSAC 1 cut(s) 1147
TspDTI ATGAA 5 cut(s) 45, 884, 893, 1048, 1063
TspRI CASTG 2 cut(s) 898, 1296
Van91I CCANNNNNTGG 1 cut(s) 355
VpaK11BI GGWCC 1 cut(s) 440
XagI CCTNNNNNAGG 4 cut(s) 435, 630, 651, 1224
XspI CTAG 2 cut(s) 1121, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.