MD06G1213000.v1.1

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000960
Physical Location & Seq
Forward (+)
34556648 .. 34558321
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1213000.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGGAAGATCAACCAAATCAGGCGGCGCCGCTGGTATTCCACCCTCAAACGTCAAAATTAGCGGCACTACATGTACATGTACAAAAACTCCACCACCATCACCACCAACCTCAACAACTCCCCCCTCCAAATAACTCTAATATAAATTCCCACGTCAACATTATTAACATACGACCTAGTCCTGATCCCGACGCCGATAATCATCAGGCAAACCCCTCTTCCTCTTCCCATGAAAATGCCGGGACATCACGCTTTGATCATCAGGAATATCATGACCACGATCACGATCATCATCAACAAGCCCGACCATTACATGTAGTACAGTTCCCACCCTCAAACCTCAATAGTGCTAATCTCATCTTGCACCAGCAGATTAACAATTGGCCTCACCATAATTACAACGCGCACGTGCCTTCACGTTCATATTATCCTCCTCCTCATCACCACCGAGCTGCTTCGGCCGACGCATTTCACCATTATCATCATCGTCACCATCGAGATGCTTCTGCTGATGCAATTTATTATAGTTCACACCAAGTGCTTTTCCCTCCTCCCGCTGCCTACCAAATTGTTCCTCATCAGCAGCTGCAGCTGCAGCTGCAGTATTATAATCATGCTTCTACCGCAAGAAATCAACTCCTGCCGGCGTTGCATTGTAACAGTCATTTTGCTGAAACAGGAGTACCCATCCAAAACATTGAATACGTACCAGTGCTGCTGCCTCTGCCAGGCATCGATGGAACTTGGAAAACAGGGCTCTTCGAGTGCATGGAGGATCCCCCAAATGCTATCATGACGCTATTGTGCCCATGCTGGACGTTCGGTCAGATTGCAGAGATCGTCAATAATGGTCGAAGCTCTTGTGCAGCCAATGCACTGATTTACATGTTGATCATGCTGTGCATCTTTGTGCCATGCTTGCTATCGTGCACATACCGGAAAAAGCTAAGGAACAAGTTCGACCTGCCCGAAAGCCCCGCTCCGGATTGCATCACTCACTTCTTATGCGAGTGGTGTGCTCTCTGCCAAGAACATAGGGAGCTCCAGCTCAGAGGCCTAGACCCTACTTTAGGATGGGTAGGAAACTTGGAGCAAATTCAGAGGATGCAGAAAAAGCGGCAAGCTGCTATGAGTCCGCCAACGACCCAAAGAATGACGGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

389

Amino Acids

44.26

Weight (kDa)

6.76

Isoelectric Point (pI)

60.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 249 - 347 6.8e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
Acc36I ACCTGC 1 cut(s) 972
AccB1I GGYRCC 1 cut(s) 25
AccBSI CCGCTC 1 cut(s) 980
AccII CGCG 1 cut(s) 404
AccIII TCCGGA 1 cut(s) 982
AciI CCGC 8 cut(s) 23, 29, 62, 555, 624, 978, 1117, 1136
AclWI GGATC 3 cut(s) 179, 770, 783
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 2 cut(s) 145, 1095
AcvI CACGTG 1 cut(s) 409
AcyI GRCGYC 2 cut(s) 26, 192
AdeI CACNNNGTG 1 cut(s) 538
AfaI GTAC 5 cut(s) 75, 81, 321, 684, 708
AfiI CCNNNNNNNGG 3 cut(s) 728, 982, 1070
AflIII ACRYGT 4 cut(s) 70, 76, 313, 885
AgsI TTSAA 1 cut(s) 701
AhdI GACNNNNNGTC 1 cut(s) 822
AjiI CACGTC 1 cut(s) 154
AjnI CCWGG 1 cut(s) 727
AluBI AGCT 9 cut(s) 452, 586, 592, 598, 858, 946, 1042, 1048, 1124
AluI AGCT 9 cut(s) 452, 586, 592, 598, 858, 946, 1042, 1048, 1124
Alw21I GWGCWC 3 cut(s) 932, 1021, 1044
Alw44I GTGCAC 1 cut(s) 928
AlwI GGATC 3 cut(s) 179, 770, 783
AlwNI CAGNNNCTG 1 cut(s) 586
Aor13HI TCCGGA 1 cut(s) 982
AoxI GGCC 3 cut(s) 383, 459, 1054
ApaLI GTGCAC 1 cut(s) 928
ApoI RAATTY 2 cut(s) 145, 1095
Asp700I GAANNNNTTC 1 cut(s) 956
AspLEI GCGC 2 cut(s) 28, 406
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 5 cut(s) 92, 380, 434, 464, 482
BaeGI GKGCMC 2 cut(s) 809, 932
BaeI ACNNNNGTAYC 2 cut(s) 666, 699
BamHI GGATCC 1 cut(s) 775
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 2 cut(s) 759, 1044
BbrPI CACGTG 1 cut(s) 409
Bbv12I GWGCWC 3 cut(s) 932, 1021, 1044
BccI CCATC 5 cut(s) 105, 501, 695, 731, 1068
BcgI CGANNNNNNTGC 2 cut(s) 998, 1032
BciT130I CCWGG 1 cut(s) 729
BclI TGATCA 2 cut(s) 256, 891
BcnI CCSGG 1 cut(s) 241
BfaI CTAG 2 cut(s) 177, 1058
BfmI CTRYAG 3 cut(s) 587, 593, 599
BfoI RGCGCY 1 cut(s) 29
BfuAI ACCTGC 1 cut(s) 972
Bme1390I CCNGG 2 cut(s) 241, 729
BmeRI GACNNNNNGTC 1 cut(s) 822
BmgBI CACGTC 1 cut(s) 154
BmiI GGNNCC 2 cut(s) 27, 777
BmrFI CCNGG 2 cut(s) 241, 729
BmsI GCATC 6 cut(s) 490, 502, 741, 912, 999, 1095
BpmI CTGGAG 1 cut(s) 1028
Bpu10I CCTNAGC 1 cut(s) 947
BpuMI CCSGG 1 cut(s) 241
Bsa29I ATCGAT 1 cut(s) 735
BsaAI YACGTR 2 cut(s) 409, 706
BsaBI GATNNNNATC 3 cut(s) 201, 285, 291
BsaHI GRCGYC 2 cut(s) 26, 192
BsaWI WCCGGW 2 cut(s) 936, 982
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 3 cut(s) 728, 982, 1070
Bse118I RCCGGY 1 cut(s) 643
Bse1I ACTGG 1 cut(s) 710
Bse8I GATNNNNATC 3 cut(s) 201, 285, 291
BseAI TCCGGA 1 cut(s) 982
BseBI CCWGG 1 cut(s) 729
BseCI ATCGAT 1 cut(s) 735
BseGI GGATG 3 cut(s) 687, 1079, 1110
BseJI GATNNNNATC 3 cut(s) 201, 285, 291
BseLI CCNNNNNNNGG 3 cut(s) 728, 982, 1070
BseMII CTCAG 1 cut(s) 1063
BseNI ACTGG 1 cut(s) 710
BseRI GAGGAG 3 cut(s) 423, 426, 540
BseSI GKGCMC 2 cut(s) 809, 932
BseX3I CGGCCG 1 cut(s) 459
BsgI GTGCAG 1 cut(s) 885
Bsh1236I CGCG 1 cut(s) 404
Bsh1285I CGRYCG 1 cut(s) 462
BshFI GGCC 3 cut(s) 385, 461, 1056
BshNI GGYRCC 1 cut(s) 25
BshVI ATCGAT 1 cut(s) 735
BsiEI CGRYCG 1 cut(s) 462
BsiHKAI GWGCWC 3 cut(s) 932, 1021, 1044
BsiSI CCGG 4 cut(s) 240, 644, 937, 983
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 3 cut(s) 728, 982, 1070
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 3 cut(s) 385, 461, 1056
Bsp1286I GDGCHC 5 cut(s) 759, 809, 932, 1021, 1044
Bsp13I TCCGGA 1 cut(s) 982
Bsp1407I TGTACA 2 cut(s) 73, 79
Bsp143I GATC 8 cut(s) 7, 184, 256, 280, 286, 775, 837, 891
BspACI CCGC 8 cut(s) 23, 29, 62, 555, 624, 978, 1117, 1136
BspANI GGCC 3 cut(s) 385, 461, 1056
BspCNI CTCAG 1 cut(s) 1062
BspDI ATCGAT 1 cut(s) 735
BspEI TCCGGA 1 cut(s) 982
BspFNI CGCG 1 cut(s) 404
BspHI TCATGA 2 cut(s) 271, 792
BspLI GGNNCC 2 cut(s) 27, 777
BspMAI CTGCAG 3 cut(s) 591, 597, 603
BspMI ACCTGC 1 cut(s) 972
BspPI GGATC 3 cut(s) 179, 770, 783
BspQI GCTCTTC 1 cut(s) 764
BspT107I GGYRCC 1 cut(s) 25
BsrBI CCGCTC 1 cut(s) 980
BsrFI RCCGGY 1 cut(s) 643
BsrGI TGTACA 2 cut(s) 73, 79
BsrI ACTGG 1 cut(s) 710
BssAI RCCGGY 1 cut(s) 643
BssMI GATC 8 cut(s) 7, 184, 256, 280, 286, 775, 837, 891
BssNI GRCGYC 2 cut(s) 26, 192
Bst2UI CCWGG 1 cut(s) 729
Bst4CI ACNGT 2 cut(s) 324, 662
Bst6I CTCTTC 3 cut(s) 223, 229, 764
BstACI GRCGYC 2 cut(s) 26, 192
BstAPI GCANNNNNTGC 1 cut(s) 872
BstAUI TGTACA 2 cut(s) 73, 79
BstBAI YACGTR 2 cut(s) 409, 706
BstC8I GCNNGC 3 cut(s) 645, 920, 1122
BstDEI CTNAG 2 cut(s) 947, 1049
BstENI CCTNNNNNAGG 2 cut(s) 726, 1068
BstF5I GGATG 3 cut(s) 687, 1079, 1110
BstFNI CGCG 1 cut(s) 404
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 406
BstKTI GATC 8 cut(s) 10, 187, 259, 283, 289, 778, 840, 894
BstMBI GATC 8 cut(s) 7, 184, 256, 280, 286, 775, 837, 891
BstMCI CGRYCG 1 cut(s) 462
BstNI CCWGG 1 cut(s) 729
BstNSI RCATGY 4 cut(s) 74, 80, 317, 889
BstSCI CCNGG 2 cut(s) 239, 727
BstSFI CTRYAG 3 cut(s) 587, 593, 599
BstSLI GKGCMC 2 cut(s) 809, 932
BstSNI TACGTA 1 cut(s) 706
BstUI CGCG 1 cut(s) 404
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
BstZI CGGCCG 1 cut(s) 459
Bsu15I ATCGAT 1 cut(s) 735
BsuRI GGCC 3 cut(s) 385, 461, 1056
BsuTUI ATCGAT 1 cut(s) 735
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 3 cut(s) 687, 1079, 1110
BtsIMutI CAGTG 2 cut(s) 717, 875
BveI ACCTGC 1 cut(s) 972
Cac8I GCNNGC 3 cut(s) 645, 920, 1122
CaiI CAGNNNCTG 1 cut(s) 586
CciI TCATGA 2 cut(s) 271, 792
CfoI GCGC 2 cut(s) 28, 406
Cfr10I RCCGGY 1 cut(s) 643
ClaI ATCGAT 1 cut(s) 735
CseI GACGC 3 cut(s) 200, 473, 805
Csp6I GTAC 5 cut(s) 74, 80, 320, 683, 707
CviQI GTAC 5 cut(s) 74, 80, 320, 683, 707
DdeI CTNAG 2 cut(s) 947, 1049
DinI GGCGCC 1 cut(s) 27
DpnI GATC 8 cut(s) 9, 186, 258, 282, 288, 777, 839, 893
DpnII GATC 8 cut(s) 7, 184, 256, 280, 286, 775, 837, 891
DraIII CACNNNGTG 1 cut(s) 538
DriI GACNNNNNGTC 1 cut(s) 822
EaeI YGGCCR 1 cut(s) 459
EagI CGGCCG 1 cut(s) 459
Eam1104I CTCTTC 3 cut(s) 223, 229, 764
Eam1105I GACNNNNNGTC 1 cut(s) 822
EarI CTCTTC 3 cut(s) 223, 229, 764
EciI GGCGGA 1 cut(s) 1125
Ecl136II GAGCTC 1 cut(s) 1042
EclXI CGGCCG 1 cut(s) 459
Eco105I TACGTA 1 cut(s) 706
Eco147I AGGCCT 1 cut(s) 1056
Eco24I GRGCYC 2 cut(s) 759, 1044
Eco52I CGGCCG 1 cut(s) 459
Eco53kI GAGCTC 1 cut(s) 1042
Eco72I CACGTG 1 cut(s) 409
EcoICRI GAGCTC 1 cut(s) 1042
EcoNI CCTNNNNNAGG 2 cut(s) 726, 1068
EcoRII CCWGG 1 cut(s) 727
EcoT38I GRGCYC 2 cut(s) 759, 1044
EgeI GGCGCC 1 cut(s) 27
EheI GGCGCC 1 cut(s) 27
FaqI GGGAC 1 cut(s) 256
FauI CCCGC 2 cut(s) 562, 985
FbaI TGATCA 2 cut(s) 256, 891
FokI GGATG 3 cut(s) 674, 1086, 1117
FriOI GRGCYC 2 cut(s) 759, 1044
FspBI CTAG 2 cut(s) 177, 1058
GlaI GCGC 2 cut(s) 27, 405
GsuI CTGGAG 1 cut(s) 1028
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 3 cut(s) 385, 461, 1056
HapII CCGG 4 cut(s) 240, 644, 937, 983
HgaI GACGC 3 cut(s) 200, 473, 805
HhaI GCGC 2 cut(s) 28, 406
Hin1I GRCGYC 2 cut(s) 26, 192
Hin6I GCGC 2 cut(s) 26, 404
HinP1I GCGC 2 cut(s) 26, 404
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HinfI GANTC 1 cut(s) 1132
HpaII CCGG 4 cut(s) 240, 644, 937, 983
HphI GGTGA 5 cut(s) 92, 380, 434, 464, 482
Hpy166II GTNNAC 3 cut(s) 157, 530, 930
Hpy188I TCNGA 3 cut(s) 828, 1052, 1101
Hpy188III TCNNGA 8 cut(s) 182, 188, 263, 272, 284, 497, 793, 983
Hpy8I GTNNAC 3 cut(s) 157, 530, 930
Hpy99I CGWCG 2 cut(s) 194, 467
HpyAV CCTTC 1 cut(s) 423
HpyCH4III ACNGT 2 cut(s) 324, 662
HpyCH4IV ACGT 6 cut(s) 50, 153, 408, 418, 705, 818
HpyF3I CTNAG 2 cut(s) 947, 1049
HpySE526I ACGT 6 cut(s) 50, 153, 408, 418, 705, 818
Hsp92I GRCGYC 2 cut(s) 26, 192
HspAI GCGC 2 cut(s) 26, 404
KasI GGCGCC 1 cut(s) 25
Kpn2I TCCGGA 1 cut(s) 982
KroI GCCGGC 1 cut(s) 643
KroNI GCCGGC 1 cut(s) 645
Ksp22I TGATCA 2 cut(s) 256, 891
Kzo9I GATC 8 cut(s) 7, 184, 256, 280, 286, 775, 837, 891
LguI GCTCTTC 1 cut(s) 764
LmnI GCTCC 4 cut(s) 985, 1039, 1047, 1090
LweI GCATC 6 cut(s) 490, 502, 741, 912, 999, 1095
MaeI CTAG 2 cut(s) 177, 1058
MaeII ACGT 6 cut(s) 50, 153, 408, 418, 705, 818
MaeIII GTNAC 2 cut(s) 488, 656
MalI GATC 8 cut(s) 9, 186, 258, 282, 288, 777, 839, 893
MbiI CCGCTC 1 cut(s) 980
MboI GATC 8 cut(s) 7, 184, 256, 280, 286, 775, 837, 891
MboII GAAGA 4 cut(s) 17, 210, 216, 751
MfeI CAATTG 1 cut(s) 379
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 5 cut(s) 759, 809, 932, 1021, 1044
MluCI AATT 7 cut(s) 56, 145, 379, 394, 516, 567, 1095
Mly113I GGCGCC 1 cut(s) 26
MlyI GAGTC 1 cut(s) 1141
MroI TCCGGA 1 cut(s) 982
MroNI GCCGGC 1 cut(s) 643
MroXI GAANNNNTTC 1 cut(s) 956
MseI TTAA 2 cut(s) 165, 375
MslI CAYNNNNRTG 4 cut(s) 75, 234, 498, 908
MspA1I CMGCKG 5 cut(s) 31, 557, 586, 592, 598
MspI CCGG 4 cut(s) 240, 644, 937, 983
MspR9I CCNGG 2 cut(s) 241, 729
MunI CAATTG 1 cut(s) 379
MvaI CCWGG 1 cut(s) 729
MvnI CGCG 1 cut(s) 404
NaeI GCCGGC 1 cut(s) 645
NarI GGCGCC 1 cut(s) 26
NciI CCSGG 1 cut(s) 241
NdeII GATC 8 cut(s) 7, 184, 256, 280, 286, 775, 837, 891
NgoMIV GCCGGC 1 cut(s) 643
NlaIV GGNNCC 2 cut(s) 27, 777
NmuCI GTSAC 1 cut(s) 488
NspI RCATGY 4 cut(s) 74, 80, 317, 889
PagI TCATGA 2 cut(s) 271, 792
PceI AGGCCT 1 cut(s) 1056
PciI ACATGT 4 cut(s) 70, 76, 313, 885
PciSI GCTCTTC 1 cut(s) 764
PcsI WCGNNNNNNNCGW 2 cut(s) 493, 966
PdiI GCCGGC 1 cut(s) 645
PdmI GAANNNNTTC 1 cut(s) 956
PflFI GACNNNGTC 1 cut(s) 177
PleI GAGTC 1 cut(s) 1140
PluTI GGCGCC 1 cut(s) 29
PmaCI CACGTG 1 cut(s) 409
PmlI CACGTG 1 cut(s) 409
PpsI GAGTC 1 cut(s) 1140
Ppu21I YACGTR 2 cut(s) 409, 706
PscI ACATGT 4 cut(s) 70, 76, 313, 885
PsiI TTATAA 1 cut(s) 609
Psp124BI GAGCTC 1 cut(s) 1044
Psp6I CCWGG 1 cut(s) 727
PspCI CACGTG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 727
PspN4I GGNNCC 2 cut(s) 27, 777
PstI CTGCAG 3 cut(s) 591, 597, 603
PstNI CAGNNNCTG 1 cut(s) 586
PsuI RGATCY 1 cut(s) 775
PsyI GACNNNGTC 1 cut(s) 177
PvuII CAGCTG 3 cut(s) 586, 592, 598
RsaI GTAC 5 cut(s) 75, 81, 321, 684, 708
RsaNI GTAC 5 cut(s) 74, 80, 320, 683, 707
RseI CAYNNNNRTG 4 cut(s) 75, 234, 498, 908
SacI GAGCTC 1 cut(s) 1044
SapI GCTCTTC 1 cut(s) 764
SaqAI TTAA 2 cut(s) 165, 375
Sau3AI GATC 8 cut(s) 7, 184, 256, 280, 286, 775, 837, 891
SchI GAGTC 1 cut(s) 1141
ScrFI CCNGG 2 cut(s) 241, 729
SduI GDGCHC 5 cut(s) 759, 809, 932, 1021, 1044
SfaNI GCATC 6 cut(s) 490, 502, 741, 912, 999, 1095
SfcI CTRYAG 3 cut(s) 587, 593, 599
SfoI GGCGCC 1 cut(s) 27
SmiMI CAYNNNNRTG 4 cut(s) 75, 234, 498, 908
SnaBI TACGTA 1 cut(s) 706
Sse9I AATT 7 cut(s) 56, 145, 379, 394, 516, 567, 1095
SseBI AGGCCT 1 cut(s) 1056
SsiI CCGC 8 cut(s) 23, 29, 62, 555, 624, 978, 1117, 1136
SspDI GGCGCC 1 cut(s) 25
SspMI CTAG 2 cut(s) 177, 1058
SstI GAGCTC 1 cut(s) 1044
StuI AGGCCT 1 cut(s) 1056
StyD4I CCNGG 2 cut(s) 239, 727
TaaI ACNGT 2 cut(s) 324, 662
TaiI ACGT 6 cut(s) 53, 156, 411, 421, 708, 821
TaqI TCGA 5 cut(s) 496, 735, 762, 853, 960
TaqII GACCGA 1 cut(s) 812
TasI AATT 7 cut(s) 56, 145, 379, 394, 516, 567, 1095
TatI WGTACW 3 cut(s) 73, 79, 319
TauI GCSGC 4 cut(s) 26, 31, 65, 1120
Tru1I TTAA 2 cut(s) 165, 375
Tru9I TTAA 2 cut(s) 165, 375
TscAI CASTG 2 cut(s) 717, 882
TseFI GTSAC 1 cut(s) 488
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 2 cut(s) 246, 411
TspRI CASTG 2 cut(s) 717, 882
Tth111I GACNNNGTC 1 cut(s) 177
VneI GTGCAC 1 cut(s) 928
XagI CCTNNNNNAGG 2 cut(s) 726, 1068
XapI RAATTY 2 cut(s) 145, 1095
XceI RCATGY 4 cut(s) 74, 80, 317, 889
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 2 cut(s) 177, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.