RLG00000004940

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000960
Physical Location & Seq
Reverse (-)
63866382 .. 63867972
1591 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004940

Sequence Viewer

Length: 1257 bp
ATGGAGGATGATCATCACACTCCAGATTACGAAGATCATCATACTCCAGATTACGAAGACCACCCCCACGATGATCAACCTGAAGCTCATGTGGAGGTACAAGTCGAACAATATCAATATCAACAAGTAGCGCTTGAAATTAGCGACAATAGTGGAAACTACGACCAAAACCAGCAGCAGCAGCAGCAGGCACCTCAGTATCAGCCACAACCTCCACCACAAGGAGGCCAACAACAACAACAGTTCTTCCAGCAGCAGCAGCCGCCGCCGCCGGGTCAACCATCTTATCAGCCGCCTGTACAATTCCCACCACAACAACCACCACCGCGGCCGATCCAATACTTGGCTACACAGCCGCCTCGCCCTGTGCCATTCCCTCCTCAGAGCCCACAAAACCAAAACTACGCAAACCAGGCTCCTCCTCCAGGACCTCCACAACAGTTCCCACCACAAAACCCTCAGTTTCAAGCTCAACAGCCGCCTTATCAGCCACAGCCACCTCAACAGTTTCCACCACAGAATGCGGCCCCTAACCAACAAAACAACCCTTTTGGACAACCACCTTCTCCGGTGAAATATCCTCCACAACCACCATCTCCGGCACAATATCCTCCTGCAGCAGGTTCGTCTCAGCCTCCTCAAGGCTACAATGACATTCCCCAACCTTCAAAGCAGCATCAGGCGCCGTCTCCGGTGCAAGTGGGCTATAATAAGCCTCAGCCACCCCAACAGTACCACCAGCAGCAACAATACCAACCACAACCACCACAGCAGAATCATTATGCGGCTGCTCCTTCTCCGGCTCAAGGAGTTCCGATGCAGGCCCCTTACGGCCCGCAGGGAACGCCTATGACTATGAATATGATGAACCCGATTGGCACTGAGGCTTGGAAACCGGGCTCTTTGATTGCATGGATGATCCTAATAATGATAGCAGAAGTTGTGGATAATGGCACTACCTCATGTGGTACGAGCGCTTTGCTGTACGGCTTGGTTGGAGCGTTCATCGGAGTGCCATTCATAATGTCATGCACTTATCGCACGAAGATTCGCAGCAAGTATGGGCTAGTTGAGACCCCTGCACCTGACTGGGTCACCCATTTGTTTTGCGAGCCATGTTCTCTTTGCCAAGAGTACAGAGAACTTAATTTGAGAGGGCTAGACCCTTCTATAGGATGGCAAGGAAATATGGCAAGGGCCATGCAGCAGCAACAACCTAAGATGATGACGCCTCCAGTGAACCAAATGATGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

419

Amino Acids

46.88

Weight (kDa)

5.24

Isoelectric Point (pI)

78.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 309 - 381 3.1e-17 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 611
AccB1I GGYRCC 2 cut(s) 190, 682
AccB7I CCANNNNNTGG 1 cut(s) 343
AccII CGCG 1 cut(s) 328
AclWI GGATC 2 cut(s) 328, 913
AcoI YGGCCR 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 102
AcyI GRCGYC 2 cut(s) 683, 1229
AfaI GTAC 6 cut(s) 99, 300, 734, 970, 986, 1136
AfeI AGCGCT 2 cut(s) 132, 976
AfiI CCNNNNNNNGG 9 cut(s) 221, 224, 272, 343, 425, 620, 641, 806, 1172
AgsI TTSAA 3 cut(s) 137, 467, 669
AjnI CCWGG 2 cut(s) 411, 424
AluBI AGCT 2 cut(s) 86, 470
AluI AGCT 2 cut(s) 86, 470
Alw26I GTCTC 3 cut(s) 633, 693, 1067
AlwI GGATC 2 cut(s) 328, 913
Aor51HI AGCGCT 2 cut(s) 132, 976
AoxI GGCC 6 cut(s) 226, 329, 525, 822, 832, 1197
AspLEI GCGC 3 cut(s) 133, 685, 977
AspS9I GGNCC 5 cut(s) 428, 526, 823, 833, 1197
AsuC2I CCSGG 2 cut(s) 273, 897
AsuHPI GGTGA 2 cut(s) 583, 1087
AvaII GGWCC 1 cut(s) 428
BaeI ACNNNNGTAYC 2 cut(s) 182, 215
BanI GGYRCC 2 cut(s) 190, 682
BanII GRGCYC 2 cut(s) 389, 902
BbsI GAAGAC 1 cut(s) 63
BbvCI CCTCAGC 1 cut(s) 717
BccI CCATC 3 cut(s) 289, 601, 1170
BceAI ACGGC 3 cut(s) 670, 847, 1003
BcgI CGANNNNNNTGC 4 cut(s) 606, 640, 961, 995
BciT130I CCWGG 2 cut(s) 413, 426
BclI TGATCA 2 cut(s) 10, 73
BcnI CCSGG 2 cut(s) 273, 897
BcoDI GTCTC 3 cut(s) 633, 693, 1067
BfaI CTAG 2 cut(s) 1067, 1160
BfmI CTRYAG 2 cut(s) 615, 1170
BfoI RGCGCY 3 cut(s) 134, 686, 978
BfuAI ACCTGC 1 cut(s) 611
Bme1390I CCNGG 4 cut(s) 273, 413, 426, 897
Bme18I GGWCC 1 cut(s) 428
BmgT120I GGNCC 5 cut(s) 428, 526, 823, 833, 1197
BmiI GGNNCC 5 cut(s) 192, 417, 528, 684, 825
BmrFI CCNGG 4 cut(s) 273, 413, 426, 897
BmrI ACTGGG 1 cut(s) 1099
BmsI GCATC 2 cut(s) 685, 807
BmuI ACTGGG 1 cut(s) 1099
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 4 cut(s) 6, 30, 408, 1218
Bpu10I CCTNAGC 1 cut(s) 717
BpuEI CTTGAG 2 cut(s) 624, 789
BpuMI CCSGG 2 cut(s) 273, 897
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 2 cut(s) 683, 1229
BsaI GGTCTC 1 cut(s) 1067
BsaJI CCNNGG 1 cut(s) 326
BsaWI WCCGGW 2 cut(s) 568, 691
Bsc4I CCNNNNNNNGG 9 cut(s) 221, 224, 272, 343, 425, 620, 641, 806, 1172
Bse1I ACTGG 2 cut(s) 1094, 1235
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 413, 426
BseDI CCNNGG 1 cut(s) 326
BseGI GGATG 3 cut(s) 13, 921, 1181
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 9 cut(s) 221, 224, 272, 343, 425, 620, 641, 806, 1172
BseMII CTCAG 6 cut(s) 209, 395, 473, 644, 731, 873
BseNI ACTGG 2 cut(s) 1094, 1235
BseRI GAGGAG 4 cut(s) 369, 408, 411, 627
BseX3I CGGCCG 1 cut(s) 329
BsgI GTGCAG 1 cut(s) 1065
Bsh1236I CGCG 1 cut(s) 328
Bsh1285I CGRYCG 1 cut(s) 332
BshFI GGCC 6 cut(s) 228, 331, 527, 824, 834, 1199
BshNI GGYRCC 2 cut(s) 190, 682
BsiEI CGRYCG 1 cut(s) 332
BsiSI CCGG 6 cut(s) 272, 569, 599, 692, 800, 896
BslI CCNNNNNNNGG 9 cut(s) 221, 224, 272, 343, 425, 620, 641, 806, 1172
BsmAI GTCTC 3 cut(s) 633, 693, 1067
BsmBI CGTCTC 2 cut(s) 633, 693
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 6 cut(s) 228, 331, 527, 824, 834, 1199
Bso31I GGTCTC 1 cut(s) 1067
Bsp1286I GDGCHC 2 cut(s) 389, 902
Bsp1407I TGTACA 1 cut(s) 298
Bsp143I GATC 5 cut(s) 10, 34, 73, 333, 918
BspANI GGCC 6 cut(s) 228, 331, 527, 824, 834, 1199
BspCNI CTCAG 6 cut(s) 208, 394, 472, 643, 730, 874
BspFNI CGCG 1 cut(s) 328
BspLI GGNNCC 5 cut(s) 192, 417, 528, 684, 825
BspMAI CTGCAG 1 cut(s) 619
BspMI ACCTGC 1 cut(s) 611
BspPI GGATC 2 cut(s) 328, 913
BspT107I GGYRCC 2 cut(s) 190, 682
BspTNI GGTCTC 1 cut(s) 1067
BsrGI TGTACA 1 cut(s) 298
BsrI ACTGG 2 cut(s) 1094, 1235
BssECI CCNNGG 1 cut(s) 326
BssMI GATC 5 cut(s) 10, 34, 73, 333, 918
BssNI GRCGYC 2 cut(s) 683, 1229
Bst2UI CCWGG 2 cut(s) 413, 426
Bst4CI ACNGT 4 cut(s) 243, 441, 507, 732
BstACI GRCGYC 2 cut(s) 683, 1229
BstAUI TGTACA 1 cut(s) 298
BstC8I GCNNGC 4 cut(s) 189, 822, 836, 1112
BstDEI CTNAG 7 cut(s) 195, 381, 459, 630, 717, 882, 1218
BstDSI CCRYGG 1 cut(s) 326
BstEII GGTNACC 1 cut(s) 1093
BstENI CCTNNNNNAGG 4 cut(s) 423, 618, 639, 1170
BstF5I GGATG 3 cut(s) 13, 921, 1181
BstFNI CGCG 1 cut(s) 328
BstH2I RGCGCY 3 cut(s) 134, 686, 978
BstHHI GCGC 3 cut(s) 133, 685, 977
BstKTI GATC 5 cut(s) 13, 37, 76, 336, 921
BstMAI GTCTC 3 cut(s) 633, 693, 1067
BstMBI GATC 5 cut(s) 10, 34, 73, 333, 918
BstMCI CGRYCG 1 cut(s) 332
BstNI CCWGG 2 cut(s) 413, 426
BstPI GGTNACC 1 cut(s) 1093
BstSCI CCNGG 4 cut(s) 271, 411, 424, 895
BstSFI CTRYAG 2 cut(s) 615, 1170
BstUI CGCG 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 63
BstZI CGGCCG 1 cut(s) 329
BsuRI GGCC 6 cut(s) 228, 331, 527, 824, 834, 1199
BtgI CCRYGG 1 cut(s) 326
BtsCI GGATG 3 cut(s) 13, 921, 1181
BtsIMutI CAGTG 2 cut(s) 879, 1242
BveI ACCTGC 1 cut(s) 611
Cac8I GCNNGC 4 cut(s) 189, 822, 836, 1112
CfoI GCGC 3 cut(s) 133, 685, 977
Cfr13I GGNCC 5 cut(s) 428, 526, 823, 833, 1197
Cfr42I CCGCGG 1 cut(s) 329
CseI GACGC 1 cut(s) 1237
Csp6I GTAC 6 cut(s) 98, 299, 733, 969, 985, 1135
CviAII CATG 6 cut(s) 89, 912, 963, 1029, 1116, 1201
CviQI GTAC 6 cut(s) 98, 299, 733, 969, 985, 1135
DdeI CTNAG 7 cut(s) 195, 381, 459, 630, 717, 882, 1218
DinI GGCGCC 1 cut(s) 684
DpnI GATC 5 cut(s) 12, 36, 75, 335, 920
DpnII GATC 5 cut(s) 10, 34, 73, 333, 918
EaeI YGGCCR 1 cut(s) 329
EagI CGGCCG 1 cut(s) 329
EclXI CGGCCG 1 cut(s) 329
Eco24I GRGCYC 2 cut(s) 389, 902
Eco31I GGTCTC 1 cut(s) 1067
Eco47I GGWCC 1 cut(s) 428
Eco47III AGCGCT 2 cut(s) 132, 976
Eco52I CGGCCG 1 cut(s) 329
Eco57I CTGAAG 1 cut(s) 102
Eco91I GGTNACC 1 cut(s) 1093
EcoNI CCTNNNNNAGG 4 cut(s) 423, 618, 639, 1170
EcoO109I RGGNCCY 2 cut(s) 428, 823
EcoO65I GGTNACC 1 cut(s) 1093
EcoRII CCWGG 2 cut(s) 411, 424
EcoT38I GRGCYC 2 cut(s) 389, 902
EgeI GGCGCC 1 cut(s) 684
EheI GGCGCC 1 cut(s) 684
Esp3I CGTCTC 2 cut(s) 633, 693
FaeI CATG 6 cut(s) 92, 915, 966, 1032, 1119, 1204
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FatI CATG 6 cut(s) 88, 911, 962, 1028, 1115, 1200
FauI CCCGC 1 cut(s) 843
FbaI TGATCA 2 cut(s) 10, 73
FokI GGATG 3 cut(s) 20, 928, 1188
FriOI GRGCYC 2 cut(s) 389, 902
FspBI CTAG 2 cut(s) 1067, 1160
GlaI GCGC 3 cut(s) 132, 684, 976
GsuI CTGGAG 4 cut(s) 6, 30, 408, 1218
HaeII RGCGCY 3 cut(s) 134, 686, 978
HaeIII GGCC 6 cut(s) 228, 331, 527, 824, 834, 1199
HapII CCGG 6 cut(s) 272, 569, 599, 692, 800, 896
HgaI GACGC 1 cut(s) 1237
HhaI GCGC 3 cut(s) 133, 685, 977
Hin1I GRCGYC 2 cut(s) 683, 1229
Hin1II CATG 6 cut(s) 92, 915, 966, 1032, 1119, 1204
Hin6I GCGC 3 cut(s) 131, 683, 975
HinP1I GCGC 3 cut(s) 131, 683, 975
HincII GTYRAC 1 cut(s) 278
HindII GTYRAC 1 cut(s) 278
HinfI GANTC 2 cut(s) 775, 1048
HpaII CCGG 6 cut(s) 272, 569, 599, 692, 800, 896
HphI GGTGA 2 cut(s) 583, 1087
Hpy166II GTNNAC 2 cut(s) 278, 1240
Hpy188I TCNGA 3 cut(s) 384, 816, 1010
Hpy188III TCNNGA 2 cut(s) 23, 47
Hpy8I GTNNAC 2 cut(s) 278, 1240
HpyAV CCTTC 4 cut(s) 573, 675, 804, 1176
HpyCH4III ACNGT 4 cut(s) 243, 441, 507, 732
HpyCH4V TGCA 7 cut(s) 617, 697, 820, 911, 1032, 1082, 1204
HpyF3I CTNAG 7 cut(s) 195, 381, 459, 630, 717, 882, 1218
Hsp92I GRCGYC 2 cut(s) 683, 1229
Hsp92II CATG 6 cut(s) 92, 915, 966, 1032, 1119, 1204
HspAI GCGC 3 cut(s) 131, 683, 975
KasI GGCGCC 1 cut(s) 682
Ksp22I TGATCA 2 cut(s) 10, 73
KspI CCGCGG 1 cut(s) 329
Kzo9I GATC 5 cut(s) 10, 34, 73, 333, 918
LmnI GCTCC 3 cut(s) 421, 796, 998
LweI GCATC 2 cut(s) 685, 807
MaeI CTAG 2 cut(s) 1067, 1160
MaeIII GTNAC 1 cut(s) 1093
MalI GATC 5 cut(s) 12, 36, 75, 335, 920
MboI GATC 5 cut(s) 10, 34, 73, 333, 918
MboII GAAGA 4 cut(s) 44, 68, 238, 1057
MhlI GDGCHC 2 cut(s) 389, 902
MluCI AATT 3 cut(s) 138, 302, 1147
Mly113I GGCGCC 1 cut(s) 683
MmeI TCCRAC 1 cut(s) 976
MseI TTAA 1 cut(s) 1146
MslI CAYNNNNRTG 1 cut(s) 1010
MspA1I CMGCKG 1 cut(s) 328
MspI CCGG 6 cut(s) 272, 569, 599, 692, 800, 896
MspR9I CCNGG 4 cut(s) 273, 413, 426, 897
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 2 cut(s) 413, 426
MvnI CGCG 1 cut(s) 328
NarI GGCGCC 1 cut(s) 683
NciI CCSGG 2 cut(s) 273, 897
NdeII GATC 5 cut(s) 10, 34, 73, 333, 918
NlaIII CATG 6 cut(s) 92, 915, 966, 1032, 1119, 1204
NlaIV GGNNCC 5 cut(s) 192, 417, 528, 684, 825
NmuCI GTSAC 1 cut(s) 1093
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 2 cut(s) 775, 1048
PflFI GACNNNGTC 1 cut(s) 1091
PflMI CCANNNNNTGG 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 424
PluTI GGCGCC 1 cut(s) 686
PpuMI RGGWCCY 1 cut(s) 428
Psp5II RGGWCCY 1 cut(s) 428
Psp6I CCWGG 2 cut(s) 411, 424
PspEI GGTNACC 1 cut(s) 1093
PspGI CCWGG 2 cut(s) 411, 424
PspN4I GGNNCC 5 cut(s) 192, 417, 528, 684, 825
PspPI GGNCC 5 cut(s) 428, 526, 823, 833, 1197
PspPPI RGGWCCY 1 cut(s) 428
PstI CTGCAG 1 cut(s) 619
PsyI GACNNNGTC 1 cut(s) 1091
RsaI GTAC 6 cut(s) 99, 300, 734, 970, 986, 1136
RsaNI GTAC 6 cut(s) 98, 299, 733, 969, 985, 1135
RseI CAYNNNNRTG 1 cut(s) 1010
SacII CCGCGG 1 cut(s) 329
SaqAI TTAA 1 cut(s) 1146
Sau3AI GATC 5 cut(s) 10, 34, 73, 333, 918
Sau96I GGNCC 5 cut(s) 428, 526, 823, 833, 1197
ScrFI CCNGG 4 cut(s) 273, 413, 426, 897
SduI GDGCHC 2 cut(s) 389, 902
SfaNI GCATC 2 cut(s) 685, 807
SfcI CTRYAG 2 cut(s) 615, 1170
SfoI GGCGCC 1 cut(s) 684
Sfr303I CCGCGG 1 cut(s) 329
SgrBI CCGCGG 1 cut(s) 329
SinI GGWCC 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 1010
SmlI CTYRAG 2 cut(s) 639, 804
SmoI CTYRAG 2 cut(s) 639, 804
Sse9I AATT 3 cut(s) 138, 302, 1147
SspDI GGCGCC 1 cut(s) 682
SspMI CTAG 2 cut(s) 1067, 1160
StyD4I CCNGG 4 cut(s) 271, 411, 424, 895
TaaI ACNGT 4 cut(s) 243, 441, 507, 732
TaqI TCGA 1 cut(s) 105
TasI AATT 3 cut(s) 138, 302, 1147
TatI WGTACW 2 cut(s) 298, 1134
TauI GCSGC 9 cut(s) 265, 268, 271, 295, 331, 358, 481, 527, 788
TfiI GAWTC 2 cut(s) 775, 1048
Tru1I TTAA 1 cut(s) 1146
Tru9I TTAA 1 cut(s) 1146
TscAI CASTG 2 cut(s) 886, 1242
TseFI GTSAC 1 cut(s) 1093
Tsp45I GTSAC 1 cut(s) 1093
TspDTI ATGAA 4 cut(s) 872, 881, 994, 1009
TspRI CASTG 2 cut(s) 886, 1242
Tth111I GACNNNGTC 1 cut(s) 1091
Van91I CCANNNNNTGG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 428
XagI CCTNNNNNAGG 4 cut(s) 423, 618, 639, 1170
XspI CTAG 2 cut(s) 1067, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.