MD06G1212900.v1.1

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000960
Physical Location & Seq
Forward (+)
34553846 .. 34555815
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1212900.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGGGAAATCCAGAAGGAGAGCCGTCTGCGTTCGAGGGTGATTATGAACCCTACCAACCTCAGGTGGAGGTGGAGTATGAGTTACAAGCTGATCAACAATTTCAATACCAACAAGTGCCTGAAACCACCAGCCAAGACCAAAACCAACAAGATCAATACCAACCAACACCACAACCGCCACCCAGTGTACCTCAAAATAATTATGACCCCCAAGCACAAGCACAACCACAATCACAGCAAGGAGTCCTCCAGCAACTACCGCCATCCCAGCCTCAGCCGGTAGGGTTCCCACCACCACAAGGTCCTGCACAACAAAACCCCTACCAGACTCCAACGCCTCAGCCAGCGCAATTCCCTCCTCAAAGCCCGCAACAGTTCCCACCACAAAACCCACAATTCCACCAACAAGCAAACTATCAGCAGCCTAGGCCTGCACAGTTTCCACCCCAACCCCAGAATAATATGCAAACCAATCCAAACCCTAATCCCATGTATGCAAATGTGCCTAACCAACCTGGGGCGGCCTACCCTCCACAAGGACCACAACCAATGGGCATGGGGAACCAACGACCTCCAGCACAGTTTCCTCCCCAATCTCCAGCACAATTTCCTCCCCAATCTCCAGCACAATATCCTCCCCAGTCTCCAGCACTATATCCTCCCAAATCTCCAACGAATTATAATGAACAATATCAGAAGCCGGCAGTGCAATTTTCCACACAGAATCAACAATACCAACAACAGCAGCCTAATAATGGTTATGCTGCTCAAGGGGTTCCAATACAGCCGAGTCCTTACCAGCAGAATGTTGGCACTGAGCCCTGGAGTTCTAAGTTATTTGATTGCATGGATGATCCAATGAATGCTCTCACAACGGCTTTCGTTCCATGCTTGACGTTTGGCCAGATAGCAGAGGTTGTGGATAACGGCACATCATCATGTGCGACCAGCGGATTGTTCTACGGATTGATTGCAGCGTTCATTGGGGTACCATTCATAATGTCATGCACGTATCGTACAAAGCTGCGCAGCAAATACAACCTAATCGAGTCCCCTGCACCCGATTGGGTCACCCATTTATTCTGCGAACCCTGCGCTCTTTGCCAAGAGTACAGGGAACTTCAAGTCAGAGGGATTGACCCTTCAATCGGATGGATAGGAAATGTGCAAAGGAATCCCAGCTTGCAGCAGCCGAAGGTTAATATGATGATGCCTCCAATGAACCAAAATATGAGTCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

415

Amino Acids

46.26

Weight (kDa)

4.43

Isoelectric Point (pI)

79.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 275 - 373 2.7e-30 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 681
Acc16I TGCGCA 1 cut(s) 1028
Acc65I GGTACC 1 cut(s) 988
AccB1I GGYRCC 1 cut(s) 988
AciI CCGC 5 cut(s) 176, 260, 368, 521, 951
AclWI GGATC 1 cut(s) 848
AcoI YGGCCR 1 cut(s) 901
AdeI CACNNNGTG 1 cut(s) 185
AfaI GTAC 4 cut(s) 189, 990, 1018, 1112
AfiI CCNNNNNNNGG 6 cut(s) 61, 299, 517, 536, 755, 1148
AgsI TTSAA 3 cut(s) 104, 1124, 1146
AjnI CCWGG 2 cut(s) 514, 821
AluBI AGCT 3 cut(s) 89, 1024, 1182
AluI AGCT 3 cut(s) 89, 1024, 1182
Alw26I GTCTC 1 cut(s) 648
AlwI GGATC 1 cut(s) 848
AoxI GGCC 3 cut(s) 428, 522, 901
ApeKI GCWGC 8 cut(s) 421, 745, 764, 974, 1024, 1029, 1186, 1189
ArsI GACNNNNNNTTYG 1 cut(s) 1219
Asp718I GGTACC 1 cut(s) 988
AspA2I CCTAGG 1 cut(s) 425
AspLEI GCGC 3 cut(s) 349, 1029, 1097
AspS9I GGNCC 2 cut(s) 302, 539
AsuHPI GGTGA 2 cut(s) 50, 1063
AvaII GGWCC 2 cut(s) 302, 539
AvrII CCTAGG 1 cut(s) 425
AxyI CCTNAGG 1 cut(s) 60
BalI TGGCCA 1 cut(s) 903
BanI GGYRCC 1 cut(s) 988
BanII GRGCYC 1 cut(s) 822
BbvCI CCTCAGC 2 cut(s) 273, 339
BbvI GCAGC 8 cut(s) 433, 751, 757, 986, 1011, 1041, 1198, 1201
BccI CCATC 2 cut(s) 271, 1146
BceAI ACGGC 3 cut(s) 7, 891, 943
BcgI CGANNNNNNTGC 2 cut(s) 1037, 1071
BciT130I CCWGG 2 cut(s) 516, 823
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 648
BfaI CTAG 1 cut(s) 426
BisI GCNGC 9 cut(s) 422, 522, 746, 765, 975, 1025, 1030, 1187, 1190
BlnI CCTAGG 1 cut(s) 425
BlsI GCNGC 9 cut(s) 423, 523, 747, 766, 976, 1026, 1031, 1188, 1191
Bme1390I CCNGG 2 cut(s) 516, 823
Bme18I GGWCC 2 cut(s) 302, 539
BmgT120I GGNCC 2 cut(s) 302, 539
BmiI GGNNCC 4 cut(s) 287, 563, 777, 990
BmrFI CCNGG 2 cut(s) 516, 823
BmrI ACTGGG 2 cut(s) 177, 634
BmsI GCATC 1 cut(s) 1200
BmuI ACTGGG 2 cut(s) 177, 634
BpmI CTGGAG 6 cut(s) 233, 558, 582, 606, 630, 844
Bpu10I CCTNAGC 2 cut(s) 273, 339
BpuEI CTTGAG 1 cut(s) 753
BsaAI YACGTR 1 cut(s) 1011
BsaJI CCNNGG 3 cut(s) 425, 515, 821
BsaXI ACNNNNNCTCC 4 cut(s) 59, 65, 89, 95
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 299, 517, 536, 755, 1148
Bse118I RCCGGY 2 cut(s) 277, 700
Bse1I ACTGG 2 cut(s) 183, 640
Bse21I CCTNAGG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 516, 823
BseDI CCNNGG 3 cut(s) 425, 515, 821
BseGI GGATG 3 cut(s) 263, 856, 1157
BseLI CCNNNNNNNGG 6 cut(s) 61, 299, 517, 536, 755, 1148
BseMII CTCAG 4 cut(s) 74, 287, 353, 807
BseNI ACTGG 2 cut(s) 183, 640
BseRI GAGGAG 1 cut(s) 348
BseXI GCAGC 8 cut(s) 433, 751, 757, 986, 1011, 1041, 1198, 1201
BseYI CCCAGC 2 cut(s) 267, 1178
BsgI GTGCAG 3 cut(s) 291, 417, 1041
BshFI GGCC 3 cut(s) 430, 524, 903
BshNI GGYRCC 1 cut(s) 988
BsiSI CCGG 2 cut(s) 278, 701
BslFI GGGAC 1 cut(s) 1036
BslI CCNNNNNNNGG 6 cut(s) 61, 299, 517, 536, 755, 1148
BsmAI GTCTC 1 cut(s) 648
BsmFI GGGAC 1 cut(s) 1036
BsmI GAATGC 1 cut(s) 868
BsnI GGCC 3 cut(s) 430, 524, 903
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 3 cut(s) 91, 151, 853
BspACI CCGC 5 cut(s) 176, 260, 368, 521, 951
BspANI GGCC 3 cut(s) 430, 524, 903
BspCNI CTCAG 4 cut(s) 73, 286, 352, 808
BspLI GGNNCC 4 cut(s) 287, 563, 777, 990
BspPI GGATC 1 cut(s) 848
BspT107I GGYRCC 1 cut(s) 988
BsrFI RCCGGY 2 cut(s) 277, 700
BsrI ACTGG 2 cut(s) 183, 640
BssAI RCCGGY 2 cut(s) 277, 700
BssECI CCNNGG 3 cut(s) 425, 515, 821
BssMI GATC 3 cut(s) 91, 151, 853
BssT1I CCWWGG 1 cut(s) 425
Bst2UI CCWGG 2 cut(s) 516, 823
Bst4CI ACNGT 3 cut(s) 375, 438, 582
BstBAI YACGTR 1 cut(s) 1011
BstC8I GCNNGC 5 cut(s) 345, 368, 432, 702, 1184
BstDEI CTNAG 5 cut(s) 60, 273, 339, 816, 831
BstEII GGTNACC 1 cut(s) 1069
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 3 cut(s) 263, 856, 1157
BstHHI GCGC 3 cut(s) 349, 1029, 1097
BstKTI GATC 3 cut(s) 94, 154, 856
BstMAI GTCTC 1 cut(s) 648
BstMBI GATC 3 cut(s) 91, 151, 853
BstMWI GCNNNNNNNGC 6 cut(s) 259, 268, 427, 706, 1092, 1101
BstNI CCWGG 2 cut(s) 516, 823
BstPI GGTNACC 1 cut(s) 1069
BstSCI CCNGG 2 cut(s) 514, 821
BstV1I GCAGC 8 cut(s) 433, 751, 757, 986, 1011, 1041, 1198, 1201
Bsu36I CCTNAGG 1 cut(s) 60
BsuRI GGCC 3 cut(s) 430, 524, 903
BtsCI GGATG 3 cut(s) 263, 856, 1157
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 3 cut(s) 190, 711, 813
Cac8I GCNNGC 5 cut(s) 345, 368, 432, 702, 1184
CfoI GCGC 3 cut(s) 349, 1029, 1097
Cfr10I RCCGGY 2 cut(s) 277, 700
Cfr13I GGNCC 2 cut(s) 302, 539
Csp6I GTAC 4 cut(s) 188, 989, 1017, 1111
CviAII CATG 6 cut(s) 490, 556, 847, 888, 939, 1005
CviQI GTAC 4 cut(s) 188, 989, 1017, 1111
DdeI CTNAG 5 cut(s) 60, 273, 339, 816, 831
DpnI GATC 3 cut(s) 93, 153, 855
DpnII GATC 3 cut(s) 91, 151, 853
DraIII CACNNNGTG 1 cut(s) 185
EaeI YGGCCR 1 cut(s) 901
Eco130I CCWWGG 1 cut(s) 425
Eco147I AGGCCT 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 822
Eco47I GGWCC 2 cut(s) 302, 539
Eco81I CCTNAGG 1 cut(s) 60
Eco91I GGTNACC 1 cut(s) 1069
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 302
EcoO65I GGTNACC 1 cut(s) 1069
EcoRII CCWGG 2 cut(s) 514, 821
EcoT14I CCWWGG 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 822
ErhI CCWWGG 1 cut(s) 425
FaeI CATG 6 cut(s) 493, 559, 850, 891, 942, 1008
FaqI GGGAC 1 cut(s) 1036
FatI CATG 6 cut(s) 489, 555, 846, 887, 938, 1004
FauI CCCGC 1 cut(s) 375
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 9 cut(s) 422, 522, 746, 765, 975, 1025, 1030, 1187, 1190
FokI GGATG 3 cut(s) 250, 863, 1164
FriOI GRGCYC 1 cut(s) 822
Fsp4HI GCNGC 9 cut(s) 422, 522, 746, 765, 975, 1025, 1030, 1187, 1190
FspBI CTAG 1 cut(s) 426
FspI TGCGCA 1 cut(s) 1028
GlaI GCGC 3 cut(s) 348, 1028, 1096
GluI GCNGC 9 cut(s) 422, 522, 746, 765, 975, 1025, 1030, 1187, 1190
GsaI CCCAGC 2 cut(s) 271, 1182
GsuI CTGGAG 6 cut(s) 233, 558, 582, 606, 630, 844
HaeIII GGCC 3 cut(s) 430, 524, 903
HapII CCGG 2 cut(s) 278, 701
HhaI GCGC 3 cut(s) 349, 1029, 1097
Hin1II CATG 6 cut(s) 493, 559, 850, 891, 942, 1008
Hin6I GCGC 3 cut(s) 347, 1027, 1095
HinP1I GCGC 3 cut(s) 347, 1027, 1095
HinfI GANTC 7 cut(s) 243, 328, 724, 790, 1049, 1174, 1234
HpaII CCGG 2 cut(s) 278, 701
HphI GGTGA 2 cut(s) 50, 1063
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 3 cut(s) 696, 1130, 1151
Hpy188III TCNNGA 1 cut(s) 11
Hpy8I GTNNAC 1 cut(s) 188
HpyAV CCTTC 3 cut(s) 8, 1152, 1189
HpyCH4III ACNGT 3 cut(s) 375, 438, 582
HpyCH4IV ACGT 2 cut(s) 896, 1010
HpyF10VI GCNNNNNNNGC 6 cut(s) 259, 268, 427, 706, 1092, 1101
HpyF3I CTNAG 5 cut(s) 60, 273, 339, 816, 831
HpySE526I ACGT 2 cut(s) 896, 1010
Hsp92II CATG 6 cut(s) 493, 559, 850, 891, 942, 1008
HspAI GCGC 3 cut(s) 347, 1027, 1095
KpnI GGTACC 1 cut(s) 992
KroI GCCGGC 1 cut(s) 700
KroNI GCCGGC 1 cut(s) 702
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 3 cut(s) 91, 151, 853
Lsp1109I GCAGC 8 cut(s) 433, 751, 757, 986, 1011, 1041, 1198, 1201
LweI GCATC 1 cut(s) 1200
MaeI CTAG 1 cut(s) 426
MaeII ACGT 2 cut(s) 896, 1010
MaeIII GTNAC 2 cut(s) 81, 1069
MalI GATC 3 cut(s) 93, 153, 855
MboI GATC 3 cut(s) 91, 151, 853
MhlI GDGCHC 1 cut(s) 822
MlsI TGGCCA 1 cut(s) 903
MluCI AATT 7 cut(s) 98, 199, 350, 395, 605, 676, 710
MluNI TGGCCA 1 cut(s) 903
MlyI GAGTC 5 cut(s) 252, 322, 799, 1058, 1243
MmeI TCCRAC 2 cut(s) 356, 695
Mox20I TGGCCA 1 cut(s) 903
MroNI GCCGGC 1 cut(s) 700
MscI TGGCCA 1 cut(s) 903
MseI TTAA 1 cut(s) 1200
MslI CAYNNNNRTG 1 cut(s) 937
Msp20I TGGCCA 1 cut(s) 903
MspA1I CMGCKG 1 cut(s) 951
MspI CCGG 2 cut(s) 278, 701
MspR9I CCNGG 2 cut(s) 516, 823
Mva1269I GAATGC 1 cut(s) 868
MvaI CCWGG 2 cut(s) 516, 823
MwoI GCNNNNNNNGC 6 cut(s) 259, 268, 427, 706, 1092, 1101
NaeI GCCGGC 1 cut(s) 702
NdeII GATC 3 cut(s) 91, 151, 853
NgoMIV GCCGGC 1 cut(s) 700
NlaIII CATG 6 cut(s) 493, 559, 850, 891, 942, 1008
NlaIV GGNNCC 4 cut(s) 287, 563, 777, 990
NmeAIII GCCGAG 1 cut(s) 813
NmuCI GTSAC 1 cut(s) 1069
NsbI TGCGCA 1 cut(s) 1028
PceI AGGCCT 1 cut(s) 430
PctI GAATGC 1 cut(s) 868
PdiI GCCGGC 1 cut(s) 702
PfeI GAWTC 2 cut(s) 724, 1174
PkrI GCNGC 9 cut(s) 423, 523, 747, 766, 976, 1026, 1031, 1188, 1191
PleI GAGTC 5 cut(s) 251, 322, 798, 1057, 1242
PpsI GAGTC 5 cut(s) 251, 322, 798, 1057, 1242
Ppu21I YACGTR 1 cut(s) 1011
PpuMI RGGWCCY 1 cut(s) 302
PsiI TTATAA 1 cut(s) 681
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 514, 821
PspEI GGTNACC 1 cut(s) 1069
PspFI CCCAGC 2 cut(s) 267, 1178
PspGI CCWGG 2 cut(s) 514, 821
PspN4I GGNNCC 4 cut(s) 287, 563, 777, 990
PspPI GGNCC 2 cut(s) 302, 539
PspPPI RGGWCCY 1 cut(s) 302
RsaI GTAC 4 cut(s) 189, 990, 1018, 1112
RsaNI GTAC 4 cut(s) 188, 989, 1017, 1111
RseI CAYNNNNRTG 1 cut(s) 937
SaqAI TTAA 1 cut(s) 1200
SatI GCNGC 9 cut(s) 422, 522, 746, 765, 975, 1025, 1030, 1187, 1190
Sau3AI GATC 3 cut(s) 91, 151, 853
Sau96I GGNCC 2 cut(s) 302, 539
SchI GAGTC 5 cut(s) 252, 322, 799, 1058, 1243
ScrFI CCNGG 2 cut(s) 516, 823
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 1 cut(s) 1200
SinI GGWCC 2 cut(s) 302, 539
SmiMI CAYNNNNRTG 1 cut(s) 937
SmlI CTYRAG 1 cut(s) 768
SmoI CTYRAG 1 cut(s) 768
Sse9I AATT 7 cut(s) 98, 199, 350, 395, 605, 676, 710
SseBI AGGCCT 1 cut(s) 430
SsiI CCGC 5 cut(s) 176, 260, 368, 521, 951
SspI AATATT 1 cut(s) 1241
SspMI CTAG 1 cut(s) 426
StuI AGGCCT 1 cut(s) 430
StyD4I CCNGG 2 cut(s) 514, 821
StyI CCWWGG 1 cut(s) 425
TaaI ACNGT 3 cut(s) 375, 438, 582
TaiI ACGT 2 cut(s) 899, 1013
TaqI TCGA 2 cut(s) 33, 1047
TasI AATT 7 cut(s) 98, 199, 350, 395, 605, 676, 710
TatI WGTACW 1 cut(s) 1110
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 724, 1174
Tru1I TTAA 1 cut(s) 1200
Tru9I TTAA 1 cut(s) 1200
TscAI CASTG 3 cut(s) 190, 711, 820
TseFI GTSAC 1 cut(s) 1069
TseI GCWGC 8 cut(s) 421, 745, 764, 974, 1024, 1029, 1186, 1189
Tsp45I GTSAC 1 cut(s) 1069
TspDTI ATGAA 6 cut(s) 60, 699, 875, 970, 985, 1235
TspGWI ACGGA 1 cut(s) 978
TspRI CASTG 3 cut(s) 190, 711, 820
VpaK11BI GGWCC 2 cut(s) 302, 539
XagI CCTNNNNNAGG 1 cut(s) 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 806
XmaJI CCTAGG 1 cut(s) 425
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.