Rroxscaffold_3G00269270

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000960
Physical Location & Seq
Reverse (-)
62184773 .. 62185563
791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269270.1

Sequence Viewer

Length: 492 bp
ATGATGCAAAATGTACAATATCTACCGCTGCTGATGCCTCAGTCCGCTACCGGCGAGGGTACTACTTGGAAAACAGGGCTCTGTGATTGCTTACTTGACCCCCCAAATGCTATAATGACAACGTTATGCCCATGTTGGACGTTCGGTCAGATTGCAGAGATTGTTGATAACGGTCAAACCTCCTGTAGTTGCAATGCTCTGATATACATGGTGATCTGGTGTTTCATCATGGTGCCATGCTTGTTGTCATGTACATACCGAAGAAAACTAAGGAACAAACTTGACCTGCCCGAGAGCCCCGGTCCCGACTGCATCATCCACTGCTTATGCGAGCTGTGTGCTCTCTGCCAAGAACATAGGGAGCTTGAGCTCAGAGGCCTCGACCCTTCATTAGGATGGGTGGGAAACATGGAGCAACTTCAGAGGATGCAACAAAAGAGACAAGGTACAGTGGTTGTTCCACCAATGACTCAAAGAATGACGGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

163

Amino Acids

18.41

Weight (kDa)

5.63

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 23 - 121 1e-28 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 294
AccB1I GGYRCC 1 cut(s) 232
AciI CCGC 2 cut(s) 26, 45
AclI AACGTT 1 cut(s) 122
AcuI CTGAAG 1 cut(s) 404
AfaI GTAC 4 cut(s) 15, 61, 253, 448
AfiI CCNNNNNNNGG 1 cut(s) 392
AhdI GACNNNNNGTC 1 cut(s) 144
AluBI AGCT 3 cut(s) 334, 364, 370
AluI AGCT 3 cut(s) 334, 364, 370
Alw21I GWGCWC 2 cut(s) 343, 372
Alw26I GTCTC 1 cut(s) 433
Ama87I CYCGRG 1 cut(s) 290
AoxI GGCC 1 cut(s) 376
ApeKI GCWGC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 302
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 1 cut(s) 302
BaeI ACNNNNGTAYC 2 cut(s) 438, 471
BanI GGYRCC 1 cut(s) 232
BanII GRGCYC 3 cut(s) 81, 299, 372
Bbv12I GWGCWC 2 cut(s) 343, 372
BbvI GCAGC 1 cut(s) 15
BccI CCATC 1 cut(s) 390
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 1 cut(s) 433
BfmI CTRYAG 1 cut(s) 184
BfuAI ACCTGC 1 cut(s) 294
BisI GCNGC 1 cut(s) 29
BlsI GCNGC 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 302
BmeRI GACNNNNNGTC 1 cut(s) 144
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 234, 304
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 3 cut(s) 24, 321, 417
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 298
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse118I RCCGGY 1 cut(s) 50
Bse3DI GCAATG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 298
BseGI GGATG 3 cut(s) 315, 401, 432
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 2 cut(s) 53, 385
BseXI GCAGC 1 cut(s) 15
BshFI GGCC 1 cut(s) 378
BshNI GGYRCC 1 cut(s) 232
BsiHKAI GWGCWC 2 cut(s) 343, 372
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 2 cut(s) 51, 300
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 433
BsmFI GGGAC 1 cut(s) 288
BsnI GGCC 1 cut(s) 378
BsoBI CYCGRG 1 cut(s) 290
Bsp1286I GDGCHC 4 cut(s) 81, 299, 343, 372
Bsp1407I TGTACA 2 cut(s) 13, 251
Bsp143I GATC 1 cut(s) 213
BspACI CCGC 2 cut(s) 26, 45
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 2 cut(s) 52, 384
BspLI GGNNCC 2 cut(s) 234, 304
BspMI ACCTGC 1 cut(s) 294
BspT107I GGYRCC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 50
BsrGI TGTACA 2 cut(s) 13, 251
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 1 cut(s) 213
Bst4CI ACNGT 2 cut(s) 173, 451
BstAUI TGTACA 2 cut(s) 13, 251
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 3 cut(s) 39, 269, 371
BstENI CCTNNNNNAGG 1 cut(s) 390
BstF5I GGATG 3 cut(s) 315, 401, 432
BstKTI GATC 1 cut(s) 216
BstMAI GTCTC 1 cut(s) 433
BstMBI GATC 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 378
BtsCI GGATG 3 cut(s) 315, 401, 432
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 2 cut(s) 319, 456
BveI ACCTGC 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 4 cut(s) 14, 60, 252, 447
CviAII CATG 6 cut(s) 132, 208, 229, 237, 249, 409
CviJI RGCY 7 cut(s) 79, 297, 334, 364, 370, 378, 486
CviKI_1 RGCY 7 cut(s) 79, 297, 334, 364, 370, 378, 486
CviQI GTAC 4 cut(s) 14, 60, 252, 447
DdeI CTNAG 3 cut(s) 39, 269, 371
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
DriI GACNNNNNGTC 1 cut(s) 144
Eam1105I GACNNNNNGTC 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 370
Eco147I AGGCCT 1 cut(s) 378
Eco24I GRGCYC 3 cut(s) 81, 299, 372
Eco47I GGWCC 1 cut(s) 302
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 404
Eco88I CYCGRG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 370
EcoNI CCTNNNNNAGG 1 cut(s) 390
EcoT38I GRGCYC 3 cut(s) 81, 299, 372
FaeI CATG 6 cut(s) 135, 211, 232, 240, 252, 412
FaqI GGGAC 1 cut(s) 288
FatI CATG 6 cut(s) 131, 207, 228, 236, 248, 408
Fnu4HI GCNGC 1 cut(s) 29
FokI GGATG 3 cut(s) 302, 408, 439
FriOI GRGCYC 3 cut(s) 81, 299, 372
Fsp4HI GCNGC 1 cut(s) 29
GluI GCNGC 1 cut(s) 29
HaeIII GGCC 1 cut(s) 378
HapII CCGG 2 cut(s) 51, 300
Hin1II CATG 6 cut(s) 135, 211, 232, 240, 252, 412
HinfI GANTC 1 cut(s) 469
HpaII CCGG 2 cut(s) 51, 300
HphI GGTGA 1 cut(s) 223
Hpy188I TCNGA 4 cut(s) 150, 201, 374, 423
Hpy188III TCNNGA 1 cut(s) 305
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 2 cut(s) 173, 451
HpyCH4IV ACGT 2 cut(s) 122, 140
HpyCH4V TGCA 5 cut(s) 7, 155, 192, 312, 430
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpyF3I CTNAG 3 cut(s) 39, 269, 371
HpySE526I ACGT 2 cut(s) 122, 140
Hsp92II CATG 6 cut(s) 135, 211, 232, 240, 252, 412
Kzo9I GATC 1 cut(s) 213
LmnI GCTCC 2 cut(s) 361, 412
LpnPI CCDG 6 cut(s) 60, 64, 196, 202, 299, 313
Lsp1109I GCAGC 1 cut(s) 15
LweI GCATC 3 cut(s) 24, 321, 417
MaeII ACGT 2 cut(s) 122, 140
MalI GATC 1 cut(s) 215
MboI GATC 1 cut(s) 213
MboII GAAGA 1 cut(s) 273
MhlI GDGCHC 4 cut(s) 81, 299, 343, 372
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 6 cut(s) 48, 49, 190, 368, 389, 417
MslI CAYNNNNRTG 2 cut(s) 230, 394
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 2 cut(s) 51, 300
MspR9I CCNGG 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 34
NciI CCSGG 1 cut(s) 300
NdeII GATC 1 cut(s) 213
NlaIII CATG 6 cut(s) 135, 211, 232, 240, 252, 412
NlaIV GGNNCC 2 cut(s) 234, 304
PceI AGGCCT 1 cut(s) 378
PkrI GCNGC 1 cut(s) 30
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
Psp124BI GAGCTC 1 cut(s) 372
Psp1406I AACGTT 1 cut(s) 122
PspN4I GGNNCC 2 cut(s) 234, 304
PspPI GGNCC 1 cut(s) 302
RsaI GTAC 4 cut(s) 15, 61, 253, 448
RsaNI GTAC 4 cut(s) 14, 60, 252, 447
RseI CAYNNNNRTG 2 cut(s) 230, 394
SacI GAGCTC 1 cut(s) 372
SatI GCNGC 1 cut(s) 29
Sau3AI GATC 1 cut(s) 213
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 4 cut(s) 81, 299, 343, 372
SetI ASST 8 cut(s) 125, 143, 182, 288, 336, 366, 372, 448
SfaNI GCATC 3 cut(s) 24, 321, 417
SfcI CTRYAG 1 cut(s) 184
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 2 cut(s) 230, 394
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SseBI AGGCCT 1 cut(s) 378
SsiI CCGC 2 cut(s) 26, 45
SstI GAGCTC 1 cut(s) 372
StuI AGGCCT 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 2 cut(s) 173, 451
TaiI ACGT 2 cut(s) 125, 143
TaqI TCGA 1 cut(s) 381
TaqII GACCGA 1 cut(s) 134
TatI WGTACW 2 cut(s) 13, 251
TscAI CASTG 2 cut(s) 326, 456
TseI GCWGC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 214, 378
TspRI CASTG 2 cut(s) 326, 456
VpaK11BI GGWCC 1 cut(s) 302
XagI CCTNNNNNAGG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.