pycom06g19060

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000960
Physical Location & Seq
Forward (+)
22582887 .. 22584596
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19060.2

Sequence Viewer

Length: 1245 bp
ATGGGAAATCCAGAAGGAGAGCCGTCTGCGTACGAGGGTGATTATGAACCCTACCAACCTCAGGTAGAGGTGGAGTATGAGTTACAAGCTGATCAACAATTTCAAAACCAACAAGTGCCTGAAACCACCAGCCAAGACCAAAACCAACAAGATCAATACCAACCACCACCACAACCGCCACCCAGTGCATCTCAAAATAATTATGACCCTCAAGCACAAGCACAAGCACAATCACAACAAGGAGTCCTCCAACAACTAACGCCATCTCAGCCTCAGCCGGTAGGCTTCCCACCACCACAAGGTCCTCCACAACAAAACCCCTACCAGACTCCAACGCCTCAGCCAGCGCAATTCCCTCCTCAAAGCCCGCAACAGTTCCCACCACAAAACCCACAATTCCACCAACAAGCAAACTATCAGCAGCCTAGGCCTGCACAGTTTCCACCCCAACCCCAGAATAATATGCAAACCAATCCAAACCCCAATCCCATGTATGCAAATGTGCTTAACCAACCTGGGGCGGCCTACCCTCCACAAGGACCACAACCAATGAGCATGGGGAACCAACGACCTCCAGCACAGTTTCTTCCCCAATCTCCAGCACAATTTCCTCCCCAATCTCCAGCACAATATCCTCCCCAGTCTCCGGCACTATATCCTCCCAAATCTCCAACGAATTATAATCAACAATATCAGAAGCCGGCAGTGCAATTTTCCACACAGAATCAACAATACCAACAACAGCAGCCTAATAATGGTTATGCTGCTCAAGGGGTTCCAATGCAGCCGAGTCCTTACCAGCAGAATGTTGGCACTGAGCCCTGGAGTTCTGAGTTATTTGATTGCATGGATGATCCAATGAATGCTCTCACAACGGCTTTCGTTCCATGCTTGACGTTTGGCCAGATAGCAGAGGTTGTGGATAACGGCACATCCTCATGTGCGACCAGCGGATTGTTCTACGGATTGATTGCAGCGTTCATTGGGATACCATTCATAATGTCATGCACATATCGCACAAAGCTGCGCAGCAAATACAACCTAATCGAGTCCCCTGCACCCGATTGGATCACCCATTTATTCTGCGAACCCTGCGCTCTTTGCCAAGAGTACAGGGAACTTCAAGTCAGAGGGATTGACCCTTCAATCGGATGGATAGGAAATGTGCAAAGGAATCCCAGCTTGCAGCAGCCGAAGGTTAATATGATGATGCCTCCAATGAACCAAAATATGAGTCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

415

Amino Acids

46.3

Weight (kDa)

4.36

Isoelectric Point (pI)

78.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 681
Acc16I TGCGCA 1 cut(s) 1028
AciI CCGC 4 cut(s) 176, 368, 521, 951
AclWI GGATC 2 cut(s) 848, 1076
AcoI YGGCCR 1 cut(s) 901
AdeI CACNNNGTG 1 cut(s) 185
AfaI GTAC 2 cut(s) 32, 1112
AfiI CCNNNNNNNGG 7 cut(s) 61, 299, 517, 536, 646, 755, 1148
AgsI TTSAA 3 cut(s) 104, 1124, 1146
AjnI CCWGG 2 cut(s) 514, 821
AluBI AGCT 3 cut(s) 89, 1024, 1182
AluI AGCT 3 cut(s) 89, 1024, 1182
Alw26I GTCTC 1 cut(s) 648
AlwI GGATC 2 cut(s) 848, 1076
AoxI GGCC 3 cut(s) 428, 522, 901
ApeKI GCWGC 9 cut(s) 421, 745, 764, 784, 974, 1024, 1029, 1186, 1189
ArsI GACNNNNNNTTYG 1 cut(s) 1219
AspA2I CCTAGG 1 cut(s) 425
AspLEI GCGC 3 cut(s) 349, 1029, 1097
AspS9I GGNCC 2 cut(s) 302, 539
AsuHPI GGTGA 2 cut(s) 50, 1063
AvaII GGWCC 2 cut(s) 302, 539
AvrII CCTAGG 1 cut(s) 425
AxyI CCTNAGG 1 cut(s) 60
BalI TGGCCA 1 cut(s) 903
BanII GRGCYC 1 cut(s) 822
BbvCI CCTCAGC 2 cut(s) 273, 339
BbvI GCAGC 9 cut(s) 433, 751, 757, 796, 986, 1011, 1041, 1198, 1201
BccI CCATC 2 cut(s) 271, 1146
BceAI ACGGC 3 cut(s) 7, 891, 943
BcgI CGANNNNNNTGC 2 cut(s) 1037, 1071
BciT130I CCWGG 2 cut(s) 516, 823
BciVI GTATCC 1 cut(s) 981
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 648
BfaI CTAG 1 cut(s) 426
BfuI GTATCC 1 cut(s) 981
BlnI CCTAGG 1 cut(s) 425
Bme1390I CCNGG 2 cut(s) 516, 823
Bme18I GGWCC 2 cut(s) 302, 539
BmgT120I GGNCC 2 cut(s) 302, 539
BmiI GGNNCC 2 cut(s) 563, 777
BmrFI CCNGG 2 cut(s) 516, 823
BmrI ACTGGG 2 cut(s) 177, 634
BmsI GCATC 2 cut(s) 197, 1200
BmuI ACTGGG 2 cut(s) 177, 634
BpmI CTGGAG 4 cut(s) 558, 582, 606, 844
Bpu10I CCTNAGC 2 cut(s) 273, 339
BpuEI CTTGAG 2 cut(s) 195, 753
BsaJI CCNNGG 3 cut(s) 425, 515, 821
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 7 cut(s) 61, 299, 517, 536, 646, 755, 1148
Bse118I RCCGGY 2 cut(s) 277, 700
Bse1I ACTGG 2 cut(s) 183, 640
Bse21I CCTNAGG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 516, 823
BseDI CCNNGG 3 cut(s) 425, 515, 821
BseGI GGATG 3 cut(s) 856, 932, 1157
BseLI CCNNNNNNNGG 7 cut(s) 61, 299, 517, 536, 646, 755, 1148
BseMII CTCAG 6 cut(s) 74, 281, 287, 353, 807, 822
BseNI ACTGG 2 cut(s) 183, 640
BseRI GAGGAG 1 cut(s) 348
BseXI GCAGC 9 cut(s) 433, 751, 757, 796, 986, 1011, 1041, 1198, 1201
BseYI CCCAGC 1 cut(s) 1178
BsgI GTGCAG 2 cut(s) 417, 1041
BshFI GGCC 3 cut(s) 430, 524, 903
BsiSI CCGG 3 cut(s) 278, 647, 701
BsiWI CGTACG 1 cut(s) 30
BslFI GGGAC 1 cut(s) 1036
BslI CCNNNNNNNGG 7 cut(s) 61, 299, 517, 536, 646, 755, 1148
BsmAI GTCTC 1 cut(s) 648
BsmFI GGGAC 1 cut(s) 1036
BsmI GAATGC 1 cut(s) 868
BsnI GGCC 3 cut(s) 430, 524, 903
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 4 cut(s) 91, 151, 853, 1068
BspACI CCGC 4 cut(s) 176, 368, 521, 951
BspANI GGCC 3 cut(s) 430, 524, 903
BspCNI CTCAG 6 cut(s) 73, 280, 286, 352, 808, 823
BspLI GGNNCC 2 cut(s) 563, 777
BspPI GGATC 2 cut(s) 848, 1076
BsrFI RCCGGY 2 cut(s) 277, 700
BsrI ACTGG 2 cut(s) 183, 640
BssAI RCCGGY 2 cut(s) 277, 700
BssECI CCNNGG 3 cut(s) 425, 515, 821
BssMI GATC 4 cut(s) 91, 151, 853, 1068
BssT1I CCWWGG 1 cut(s) 425
Bst2UI CCWGG 2 cut(s) 516, 823
Bst4CI ACNGT 3 cut(s) 375, 438, 582
BstC8I GCNNGC 5 cut(s) 345, 368, 432, 702, 1184
BstDEI CTNAG 6 cut(s) 60, 267, 273, 339, 816, 831
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 3 cut(s) 856, 932, 1157
BstHHI GCGC 3 cut(s) 349, 1029, 1097
BstKTI GATC 4 cut(s) 94, 154, 856, 1071
BstMAI GTCTC 1 cut(s) 648
BstMBI GATC 4 cut(s) 91, 151, 853, 1068
BstMWI GCNNNNNNNGC 6 cut(s) 268, 427, 706, 1014, 1092, 1101
BstNI CCWGG 2 cut(s) 516, 823
BstSCI CCNGG 2 cut(s) 514, 821
BstV1I GCAGC 9 cut(s) 433, 751, 757, 796, 986, 1011, 1041, 1198, 1201
Bsu36I CCTNAGG 1 cut(s) 60
BsuI GTATCC 1 cut(s) 981
BsuRI GGCC 3 cut(s) 430, 524, 903
BtsCI GGATG 3 cut(s) 856, 932, 1157
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 3 cut(s) 190, 711, 813
Cac8I GCNNGC 5 cut(s) 345, 368, 432, 702, 1184
CfoI GCGC 3 cut(s) 349, 1029, 1097
Cfr10I RCCGGY 2 cut(s) 277, 700
Cfr13I GGNCC 2 cut(s) 302, 539
Csp6I GTAC 2 cut(s) 31, 1111
CviAII CATG 6 cut(s) 490, 556, 847, 888, 939, 1005
CviQI GTAC 2 cut(s) 31, 1111
DdeI CTNAG 6 cut(s) 60, 267, 273, 339, 816, 831
DpnI GATC 4 cut(s) 93, 153, 855, 1070
DpnII GATC 4 cut(s) 91, 151, 853, 1068
DraIII CACNNNGTG 1 cut(s) 185
EaeI YGGCCR 1 cut(s) 901
Eco130I CCWWGG 1 cut(s) 425
Eco147I AGGCCT 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 822
Eco47I GGWCC 2 cut(s) 302, 539
Eco81I CCTNAGG 1 cut(s) 60
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 302
EcoRII CCWGG 2 cut(s) 514, 821
EcoT14I CCWWGG 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 822
ErhI CCWWGG 1 cut(s) 425
FaeI CATG 6 cut(s) 493, 559, 850, 891, 942, 1008
FaqI GGGAC 1 cut(s) 1036
FatI CATG 6 cut(s) 489, 555, 846, 887, 938, 1004
FauI CCCGC 1 cut(s) 375
FbaI TGATCA 1 cut(s) 91
FokI GGATG 3 cut(s) 863, 919, 1164
FriOI GRGCYC 1 cut(s) 822
FspBI CTAG 1 cut(s) 426
FspI TGCGCA 1 cut(s) 1028
GlaI GCGC 3 cut(s) 348, 1028, 1096
GsaI CCCAGC 1 cut(s) 1182
GsuI CTGGAG 4 cut(s) 558, 582, 606, 844
HaeIII GGCC 3 cut(s) 430, 524, 903
HapII CCGG 3 cut(s) 278, 647, 701
HhaI GCGC 3 cut(s) 349, 1029, 1097
Hin1II CATG 6 cut(s) 493, 559, 850, 891, 942, 1008
Hin6I GCGC 3 cut(s) 347, 1027, 1095
HinP1I GCGC 3 cut(s) 347, 1027, 1095
HinfI GANTC 7 cut(s) 243, 328, 724, 790, 1049, 1174, 1234
HpaII CCGG 3 cut(s) 278, 647, 701
HphI GGTGA 2 cut(s) 50, 1063
Hpy188I TCNGA 4 cut(s) 696, 832, 1130, 1151
Hpy188III TCNNGA 1 cut(s) 11
HpyAV CCTTC 3 cut(s) 8, 1152, 1189
HpyCH4III ACNGT 3 cut(s) 375, 438, 582
HpyCH4IV ACGT 1 cut(s) 896
HpyF10VI GCNNNNNNNGC 6 cut(s) 268, 427, 706, 1014, 1092, 1101
HpyF3I CTNAG 6 cut(s) 60, 267, 273, 339, 816, 831
HpySE526I ACGT 1 cut(s) 896
Hsp92II CATG 6 cut(s) 493, 559, 850, 891, 942, 1008
HspAI GCGC 3 cut(s) 347, 1027, 1095
KroI GCCGGC 1 cut(s) 700
KroNI GCCGGC 1 cut(s) 702
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 4 cut(s) 91, 151, 853, 1068
Lsp1109I GCAGC 9 cut(s) 433, 751, 757, 796, 986, 1011, 1041, 1198, 1201
LweI GCATC 2 cut(s) 197, 1200
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 896
MaeIII GTNAC 1 cut(s) 81
MalI GATC 4 cut(s) 93, 153, 855, 1070
MboI GATC 4 cut(s) 91, 151, 853, 1068
MboII GAAGA 1 cut(s) 578
MhlI GDGCHC 1 cut(s) 822
MlsI TGGCCA 1 cut(s) 903
MluCI AATT 7 cut(s) 98, 199, 350, 395, 605, 676, 710
MluNI TGGCCA 1 cut(s) 903
MlyI GAGTC 5 cut(s) 252, 322, 799, 1058, 1243
MmeI TCCRAC 3 cut(s) 274, 356, 695
Mox20I TGGCCA 1 cut(s) 903
MroNI GCCGGC 1 cut(s) 700
MscI TGGCCA 1 cut(s) 903
MseI TTAA 2 cut(s) 507, 1200
MslI CAYNNNNRTG 1 cut(s) 937
Msp20I TGGCCA 1 cut(s) 903
MspA1I CMGCKG 1 cut(s) 951
MspI CCGG 3 cut(s) 278, 647, 701
MspR9I CCNGG 2 cut(s) 516, 823
Mva1269I GAATGC 1 cut(s) 868
MvaI CCWGG 2 cut(s) 516, 823
MwoI GCNNNNNNNGC 6 cut(s) 268, 427, 706, 1014, 1092, 1101
NaeI GCCGGC 1 cut(s) 702
NdeII GATC 4 cut(s) 91, 151, 853, 1068
NgoMIV GCCGGC 1 cut(s) 700
NlaIII CATG 6 cut(s) 493, 559, 850, 891, 942, 1008
NlaIV GGNNCC 2 cut(s) 563, 777
NmeAIII GCCGAG 1 cut(s) 813
NsbI TGCGCA 1 cut(s) 1028
PceI AGGCCT 1 cut(s) 430
PctI GAATGC 1 cut(s) 868
PdiI GCCGGC 1 cut(s) 702
PfeI GAWTC 2 cut(s) 724, 1174
Pfl23II CGTACG 1 cut(s) 30
PleI GAGTC 5 cut(s) 251, 322, 798, 1057, 1242
PpsI GAGTC 5 cut(s) 251, 322, 798, 1057, 1242
PpuMI RGGWCCY 1 cut(s) 302
PsiI TTATAA 1 cut(s) 681
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 514, 821
PspFI CCCAGC 1 cut(s) 1178
PspGI CCWGG 2 cut(s) 514, 821
PspLI CGTACG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 563, 777
PspPI GGNCC 2 cut(s) 302, 539
PspPPI RGGWCCY 1 cut(s) 302
RsaI GTAC 2 cut(s) 32, 1112
RsaNI GTAC 2 cut(s) 31, 1111
RseI CAYNNNNRTG 1 cut(s) 937
SaqAI TTAA 2 cut(s) 507, 1200
Sau3AI GATC 4 cut(s) 91, 151, 853, 1068
Sau96I GGNCC 2 cut(s) 302, 539
SchI GAGTC 5 cut(s) 252, 322, 799, 1058, 1243
ScrFI CCNGG 2 cut(s) 516, 823
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 2 cut(s) 197, 1200
SinI GGWCC 2 cut(s) 302, 539
SmiMI CAYNNNNRTG 1 cut(s) 937
SmlI CTYRAG 2 cut(s) 210, 768
SmoI CTYRAG 2 cut(s) 210, 768
Sse9I AATT 7 cut(s) 98, 199, 350, 395, 605, 676, 710
SseBI AGGCCT 1 cut(s) 430
SsiI CCGC 4 cut(s) 176, 368, 521, 951
SspI AATATT 1 cut(s) 1241
SspMI CTAG 1 cut(s) 426
StuI AGGCCT 1 cut(s) 430
StyD4I CCNGG 2 cut(s) 514, 821
StyI CCWWGG 1 cut(s) 425
TaaI ACNGT 3 cut(s) 375, 438, 582
TaiI ACGT 1 cut(s) 899
TaqI TCGA 1 cut(s) 1047
TasI AATT 7 cut(s) 98, 199, 350, 395, 605, 676, 710
TatI WGTACW 1 cut(s) 1110
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 724, 1174
Tru1I TTAA 2 cut(s) 507, 1200
Tru9I TTAA 2 cut(s) 507, 1200
TscAI CASTG 3 cut(s) 190, 711, 820
TseI GCWGC 9 cut(s) 421, 745, 764, 784, 974, 1024, 1029, 1186, 1189
TspDTI ATGAA 5 cut(s) 60, 875, 970, 985, 1235
TspGWI ACGGA 1 cut(s) 978
TspRI CASTG 3 cut(s) 190, 711, 820
VpaK11BI GGWCC 2 cut(s) 302, 539
XagI CCTNNNNNAGG 1 cut(s) 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 806
XmaJI CCTAGG 1 cut(s) 425
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.