Rroxscaffold_5G00339480

Cell number regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000960
Physical Location & Seq
Forward (+)
7946132 .. 7947235
1104 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00339480.1

Sequence Viewer

Length: 417 bp
ATGAATATTTTCAATCTCCATCGCCCCAAGCCCGAATGGTCTATCGGCCTTTGTCAATGCTTCAACGATATCCCTACATGTTGCATAACATGCCTTCTCCCATGTATCACCTTCGGCCGAATTGCAGAAATTGTGGATGAAGGACAAACTTCATGCTTTAACCAAGGCTGCGTCTACGGATTGTTGATGATGGTGCAATTTCAATGGCTCTATTCGTGCATCTATAGAGAGAAAATGCGCAAAAAGTTCAGTTTGCCGGAACAACCTTGTTGCGATTGTTGCGTTCATTTCTGCTGCGAATTATGTGCCCTTTGTCAAGAGCACGCGGAGCTCAAAGCTAGAGGCTTTGATCCCTCCATAGGTTGGACTGGGCAACCAAATGCTGTTCCCAAGATGCATTTCTCCATGTTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

138

Amino Acids

15.87

Weight (kDa)

6.84

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 13 - 110 2e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 239
AccB7I CCANNNNNTGG 1 cut(s) 363
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 326
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 115
AfiI CCNNNNNNNGG 2 cut(s) 359, 363
AflIII ACRYGT 1 cut(s) 77
AgsI TTSAA 3 cut(s) 13, 64, 203
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 2 cut(s) 331, 338
AluI AGCT 2 cut(s) 331, 338
Alw21I GWGCWC 2 cut(s) 324, 333
AlwI GGATC 1 cut(s) 344
AoxI GGCC 2 cut(s) 46, 115
ApeKI GCWGC 2 cut(s) 168, 294
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 240
AsuHPI GGTGA 1 cut(s) 100
BaeGI GKGCMC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 2 cut(s) 324, 333
BbvI GCAGC 2 cut(s) 155, 281
BccI CCATC 2 cut(s) 27, 184
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BfaI CTAG 1 cut(s) 339
BfmI CTRYAG 1 cut(s) 223
BisI GCNGC 2 cut(s) 169, 295
BlsI GCNGC 2 cut(s) 170, 296
BmrI ACTGGG 1 cut(s) 378
BmsI GCATC 2 cut(s) 228, 384
BmuI ACTGGG 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 163
Bsc4I CCNNNNNNNGG 2 cut(s) 359, 363
Bse1I ACTGG 1 cut(s) 373
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 2 cut(s) 359, 363
BseNI ACTGG 1 cut(s) 373
BseSI GKGCMC 1 cut(s) 310
BseX3I CGGCCG 1 cut(s) 115
BseXI GCAGC 2 cut(s) 155, 281
Bsh1236I CGCG 1 cut(s) 326
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 48, 117
BsiEI CGRYCG 1 cut(s) 118
BsiHKAI GWGCWC 2 cut(s) 324, 333
BsiSI CCGG 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 359, 363
BsnI GGCC 2 cut(s) 48, 117
Bsp1286I GDGCHC 3 cut(s) 310, 324, 333
Bsp143I GATC 1 cut(s) 349
BspACI CCGC 1 cut(s) 326
BspANI GGCC 2 cut(s) 48, 117
BspFNI CGCG 1 cut(s) 326
BspPI GGATC 1 cut(s) 344
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 163
BstAPI GCANNNNNTGC 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 324
BstF5I GGATG 1 cut(s) 142
BstFNI CGCG 1 cut(s) 326
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 1 cut(s) 352
BstMBI GATC 1 cut(s) 349
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 3 cut(s) 90, 279, 328
BstNSI RCATGY 2 cut(s) 81, 93
BstSFI CTRYAG 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 310
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 2 cut(s) 155, 281
BstZI CGGCCG 1 cut(s) 115
BsuRI GGCC 2 cut(s) 48, 117
BtgZI GCGATG 1 cut(s) 5
BtsCI GGATG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 240
CseI GACGC 1 cut(s) 160
CviAII CATG 5 cut(s) 78, 90, 102, 153, 406
CviJI RGCY 8 cut(s) 31, 48, 117, 168, 208, 331, 338, 345
CviKI_1 RGCY 8 cut(s) 31, 48, 117, 168, 208, 331, 338, 345
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
DraI TTTAAA 1 cut(s) 412
EaeI YGGCCR 1 cut(s) 115
EagI CGGCCG 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 331
EclXI CGGCCG 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 70
Eco52I CGGCCG 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 331
EcoRV GATATC 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 163
EcoT22I ATGCAT 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 5 cut(s) 81, 93, 105, 156, 409
FaiI YATR 9 cut(s) 79, 86, 91, 103, 154, 225, 304, 359, 407
FatI CATG 5 cut(s) 77, 89, 101, 152, 405
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 169, 295
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 2 cut(s) 169, 295
FspBI CTAG 1 cut(s) 339
FspI TGCGCA 1 cut(s) 239
GlaI GCGC 1 cut(s) 239
GluI GCNGC 2 cut(s) 169, 295
HaeIII GGCC 2 cut(s) 48, 117
HapII CCGG 1 cut(s) 257
HgaI GACGC 1 cut(s) 160
HhaI GCGC 1 cut(s) 240
Hin1II CATG 5 cut(s) 81, 93, 105, 156, 409
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HpaII CCGG 1 cut(s) 257
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 3 cut(s) 104, 121, 134
HpyCH4V TGCA 5 cut(s) 84, 125, 196, 219, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 279, 328
Hsp92II CATG 5 cut(s) 81, 93, 105, 156, 409
HspAI GCGC 1 cut(s) 238
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 2 cut(s) 270, 354
Lsp1109I GCAGC 2 cut(s) 155, 281
LweI GCATC 2 cut(s) 228, 384
MaeI CTAG 1 cut(s) 339
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MhlI GDGCHC 3 cut(s) 310, 324, 333
MluCI AATT 4 cut(s) 120, 129, 197, 299
MmeI TCCRAC 1 cut(s) 344
MnlI CCTC 2 cut(s) 335, 364
Mph1103I ATGCAT 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 159, 411
MspI CCGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 3 cut(s) 90, 279, 328
NdeII GATC 1 cut(s) 349
NlaIII CATG 5 cut(s) 81, 93, 105, 156, 409
NsbI TGCGCA 1 cut(s) 239
NsiI ATGCAT 1 cut(s) 399
NspI RCATGY 2 cut(s) 81, 93
PciI ACATGT 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 363
PkrI GCNGC 2 cut(s) 170, 296
PscI ACATGT 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 333
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 159, 411
SatI GCNGC 2 cut(s) 169, 295
Sau3AI GATC 1 cut(s) 349
SduI GDGCHC 3 cut(s) 310, 324, 333
SetI ASST 5 cut(s) 113, 268, 333, 340, 364
SfaNI GCATC 2 cut(s) 228, 384
SfcI CTRYAG 1 cut(s) 223
Sse9I AATT 4 cut(s) 120, 129, 197, 299
SsiI CCGC 1 cut(s) 326
SspI AATATT 1 cut(s) 7
SspMI CTAG 1 cut(s) 339
SstI GAGCTC 1 cut(s) 333
StyI CCWWGG 1 cut(s) 163
TasI AATT 4 cut(s) 120, 129, 197, 299
Tru1I TTAA 2 cut(s) 159, 411
Tru9I TTAA 2 cut(s) 159, 411
TseI GCWGC 2 cut(s) 168, 294
TspDTI ATGAA 4 cut(s) 17, 141, 153, 275
TspGWI ACGGA 1 cut(s) 192
Van91I CCANNNNNTGG 1 cut(s) 363
XceI RCATGY 2 cut(s) 81, 93
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 339
Zsp2I ATGCAT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.