Rw7G005870

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000960
Physical Location & Seq
Forward (+)
4919117 .. 4919856
740 bp
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UTR
Exon/CDS
Intron
Rw7G005870.1

Sequence Viewer

Length: 378 bp
ATGACTATGAATATGATGAACCCAATTGGCACTGAGGCTTGGAAAACCGGGCTCTTTGATTGCATGGATGATCCTAATAATGCATGTGGTACGAGCGCATTGCTGTACGGCTTGGTTGGAGCGTTCATCGGAGTGCCATTCATAATGTCATGCACTTATCGCACGAAGATTCGGAGCAAGTATGGGCTAGTTGAGACCCCTGCACCTGACTGGGTCACCCATTTGTTTTGCGAGCCATGTTCTCTTTGCCAAGAGTACAGAGAACTTAATTTGAGAGGCCTAGACCCTTCTATAGGATGGCAAGGAAATGTGGCAAGGGCCATGCAGCAGCAACAACCTAAGATGATGACGCCTCCAGTGAACCAAATGATGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

125

Amino Acids

14.06

Weight (kDa)

6.53

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 39 - 88 3.6e-15 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 350
AfaI GTAC 3 cut(s) 91, 107, 257
AfiI CCNNNNNNNGG 1 cut(s) 293
Alw26I GTCTC 1 cut(s) 188
AlwI GGATC 1 cut(s) 65
AoxI GGCC 2 cut(s) 277, 318
ApeKI GCWGC 2 cut(s) 325, 328
AspLEI GCGC 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 318
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 1 cut(s) 208
BanII GRGCYC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 337, 340
BccI CCATC 1 cut(s) 291
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 188
BfaI CTAG 2 cut(s) 188, 281
BfmI CTRYAG 1 cut(s) 291
BisI GCNGC 2 cut(s) 326, 329
BlsI GCNGC 2 cut(s) 327, 330
Bme1390I CCNGG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 49
BmrI ACTGGG 1 cut(s) 220
BmuI ACTGGG 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 49
BsaHI GRCGYC 1 cut(s) 350
BsaI GGTCTC 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 2 cut(s) 215, 356
Bse3DI GCAATG 1 cut(s) 98
BseGI GGATG 2 cut(s) 73, 302
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 98
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 2 cut(s) 215, 356
BseXI GCAGC 2 cut(s) 337, 340
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 2 cut(s) 279, 320
BsiSI CCGG 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 188
BsnI GGCC 2 cut(s) 279, 320
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 1 cut(s) 70
BspANI GGCC 2 cut(s) 279, 320
BspCNI CTCAG 1 cut(s) 25
BspPI GGATC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 98
BsrI ACTGG 2 cut(s) 215, 356
BssMI GATC 1 cut(s) 70
BssNI GRCGYC 1 cut(s) 350
BstACI GRCGYC 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 2 cut(s) 33, 339
BstEII GGTNACC 1 cut(s) 214
BstENI CCTNNNNNAGG 1 cut(s) 291
BstF5I GGATG 2 cut(s) 73, 302
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 1 cut(s) 70
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 87
BstPI GGTNACC 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 47
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 337, 340
BsuRI GGCC 2 cut(s) 279, 320
BtsCI GGATG 2 cut(s) 73, 302
BtsIMutI CAGTG 2 cut(s) 30, 363
Cac8I GCNNGC 1 cut(s) 233
CfoI GCGC 1 cut(s) 98
Cfr13I GGNCC 1 cut(s) 318
CseI GACGC 1 cut(s) 358
Csp6I GTAC 3 cut(s) 90, 106, 256
CviAII CATG 5 cut(s) 64, 84, 150, 237, 322
CviJI RGCY 7 cut(s) 38, 52, 111, 187, 235, 279, 320
CviKI_1 RGCY 7 cut(s) 38, 52, 111, 187, 235, 279, 320
CviQI GTAC 3 cut(s) 90, 106, 256
DdeI CTNAG 2 cut(s) 33, 339
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
Eco147I AGGCCT 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 188
Eco91I GGTNACC 1 cut(s) 214
EcoNI CCTNNNNNAGG 1 cut(s) 291
EcoO65I GGTNACC 1 cut(s) 214
EcoT22I ATGCAT 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 54
FaeI CATG 5 cut(s) 67, 87, 153, 240, 325
FatI CATG 5 cut(s) 63, 83, 149, 236, 321
Fnu4HI GCNGC 2 cut(s) 326, 329
FokI GGATG 2 cut(s) 80, 309
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 2 cut(s) 326, 329
FspBI CTAG 2 cut(s) 188, 281
GlaI GCGC 1 cut(s) 97
GluI GCNGC 2 cut(s) 326, 329
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 2 cut(s) 279, 320
HapII CCGG 1 cut(s) 48
HgaI GACGC 1 cut(s) 358
HhaI GCGC 1 cut(s) 98
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 5 cut(s) 67, 87, 153, 240, 325
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HinfI GANTC 1 cut(s) 169
HpaII CCGG 1 cut(s) 48
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 2 cut(s) 131, 174
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 1 cut(s) 297
HpyCH4V TGCA 5 cut(s) 63, 83, 153, 203, 325
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 2 cut(s) 33, 339
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 5 cut(s) 67, 87, 153, 240, 325
HspAI GCGC 1 cut(s) 96
Kzo9I GATC 1 cut(s) 70
LmnI GCTCC 2 cut(s) 119, 174
LpnPI CCDG 5 cut(s) 61, 196, 213, 219, 369
Lsp1109I GCAGC 2 cut(s) 337, 340
MaeI CTAG 2 cut(s) 188, 281
MaeIII GTNAC 1 cut(s) 214
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 1 cut(s) 178
MfeI CAATTG 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 2 cut(s) 24, 268
MmeI TCCRAC 1 cut(s) 97
MnlI CCTC 4 cut(s) 28, 269, 363, 366
Mph1103I ATGCAT 1 cut(s) 85
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 131
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 49
MunI CAATTG 1 cut(s) 24
MwoI GCNNNNNNNGC 1 cut(s) 159
NciI CCSGG 1 cut(s) 49
NdeII GATC 1 cut(s) 70
NlaIII CATG 5 cut(s) 67, 87, 153, 240, 325
NmuCI GTSAC 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 1 cut(s) 87
PceI AGGCCT 1 cut(s) 279
PfeI GAWTC 1 cut(s) 169
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 2 cut(s) 327, 330
PspEI GGTNACC 1 cut(s) 214
PspPI GGNCC 1 cut(s) 318
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 3 cut(s) 91, 107, 257
RsaNI GTAC 3 cut(s) 90, 106, 256
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 2 cut(s) 326, 329
Sau3AI GATC 1 cut(s) 70
Sau96I GGNCC 1 cut(s) 318
ScrFI CCNGG 1 cut(s) 49
SduI GDGCHC 1 cut(s) 54
SetI ASST 3 cut(s) 208, 340, 377
SfcI CTRYAG 1 cut(s) 291
SmiMI CAYNNNNRTG 1 cut(s) 131
Sse9I AATT 2 cut(s) 24, 268
SseBI AGGCCT 1 cut(s) 279
SspMI CTAG 2 cut(s) 188, 281
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 1 cut(s) 47
TasI AATT 2 cut(s) 24, 268
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 37, 363
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 2 cut(s) 325, 328
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 4 cut(s) 23, 32, 115, 130
TspRI CASTG 2 cut(s) 37, 363
Tth111I GACNNNGTC 1 cut(s) 212
XagI CCTNNNNNAGG 1 cut(s) 291
XceI RCATGY 1 cut(s) 87
XspI CTAG 2 cut(s) 188, 281
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.