RchiOBHm_Chr4g0394461

Cell number regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000960
Physical Location & Seq
Forward (+)
10128606 .. 10129590
985 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36696

Sequence Viewer

Length: 444 bp
ATGAATATTTTCAATCTCCATCGCCCCAAGCCCGAATGGTCCGTCGGCCTTTGTCAATGCTTCAACGATATTCCTACATGTTGCATAACATGCCTTCTCCCATGTATCACCTTCGGCCGAATTGCAGAAATTGTGGATGAAGGACAAACTTCATGCTTTAACCAAGGCTGCGTCTACGGATTGTTGATGATGGTGCAATTTCAATGGCTCTATTCGTGCATCTATAGAGAGAAAATGCGCAAAAAGTTCAGTTTGCCGGAACAACCTTGTTGCGATTGTTGCGTTAATTTCTGCTGCGAATTATGTGCCCTTTGTCAAGAGCACGCGGAGCTCAAAGCTAGAGGATTTGATCCCTCCATCGGTACGTACAATGAATTGTATCAATTCCATTGTTATAATCAAAGTTATCTAGCAGTTATGTTATTTGTTGTTCTGTTATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

147

Amino Acids

16.98

Weight (kDa)

5.53

Isoelectric Point (pI)

24.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 13 - 110 1.2e-30 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
Acc16I TGCGCA 1 cut(s) 239
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 326
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 115
AfaI GTAC 2 cut(s) 364, 368
AfiI CCNNNNNNNGG 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 77
AgsI TTSAA 3 cut(s) 13, 64, 203
AluBI AGCT 2 cut(s) 331, 338
AluI AGCT 2 cut(s) 331, 338
Alw21I GWGCWC 2 cut(s) 324, 333
AlwI GGATC 1 cut(s) 344
AoxI GGCC 2 cut(s) 46, 115
ApeKI GCWGC 2 cut(s) 168, 294
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 1 cut(s) 39
AsuHPI GGTGA 1 cut(s) 100
AvaII GGWCC 1 cut(s) 39
BaeGI GKGCMC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 2 cut(s) 324, 333
BbvI GCAGC 2 cut(s) 155, 281
BccI CCATC 3 cut(s) 27, 184, 365
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BfaI CTAG 2 cut(s) 339, 410
BfmI CTRYAG 1 cut(s) 223
BisI GCNGC 2 cut(s) 169, 295
BlsI GCNGC 2 cut(s) 170, 296
Bme18I GGWCC 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 39
BmsI GCATC 1 cut(s) 228
BsaAI YACGTR 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 163
Bsc4I CCNNNNNNNGG 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 1 cut(s) 359
BseSI GKGCMC 1 cut(s) 310
BseX3I CGGCCG 1 cut(s) 115
BseXI GCAGC 2 cut(s) 155, 281
Bsh1236I CGCG 1 cut(s) 326
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 48, 117
BsiEI CGRYCG 1 cut(s) 118
BsiHKAI GWGCWC 2 cut(s) 324, 333
BsiSI CCGG 1 cut(s) 257
BslI CCNNNNNNNGG 1 cut(s) 359
BsnI GGCC 2 cut(s) 48, 117
Bsp1286I GDGCHC 3 cut(s) 310, 324, 333
Bsp143I GATC 1 cut(s) 349
BspACI CCGC 1 cut(s) 326
BspANI GGCC 2 cut(s) 48, 117
BspFNI CGCG 1 cut(s) 326
BspPI GGATC 1 cut(s) 344
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 163
BstAPI GCANNNNNTGC 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 324
BstF5I GGATG 1 cut(s) 142
BstFNI CGCG 1 cut(s) 326
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 1 cut(s) 352
BstMBI GATC 1 cut(s) 349
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 3 cut(s) 90, 279, 328
BstNSI RCATGY 2 cut(s) 81, 93
BstSFI CTRYAG 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 310
BstSNI TACGTA 1 cut(s) 366
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 2 cut(s) 155, 281
BstZI CGGCCG 1 cut(s) 115
BsuRI GGCC 2 cut(s) 48, 117
BtgZI GCGATG 1 cut(s) 5
BtsCI GGATG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 39
CseI GACGC 1 cut(s) 160
Csp6I GTAC 2 cut(s) 363, 367
CviAII CATG 4 cut(s) 78, 90, 102, 153
CviJI RGCY 7 cut(s) 31, 48, 117, 168, 208, 331, 338
CviKI_1 RGCY 7 cut(s) 31, 48, 117, 168, 208, 331, 338
CviQI GTAC 2 cut(s) 363, 367
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 115
EagI CGGCCG 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 331
EclXI CGGCCG 1 cut(s) 115
Eco105I TACGTA 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 39
Eco52I CGGCCG 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 331
EcoT14I CCWWGG 1 cut(s) 163
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 4 cut(s) 81, 93, 105, 156
FaiI YATR 9 cut(s) 79, 86, 91, 103, 154, 225, 304, 396, 419
FatI CATG 4 cut(s) 77, 89, 101, 152
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 169, 295
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 2 cut(s) 169, 295
FspBI CTAG 2 cut(s) 339, 410
FspI TGCGCA 1 cut(s) 239
GlaI GCGC 1 cut(s) 239
GluI GCNGC 2 cut(s) 169, 295
HaeIII GGCC 2 cut(s) 48, 117
HapII CCGG 1 cut(s) 257
HgaI GACGC 1 cut(s) 160
HhaI GCGC 1 cut(s) 240
Hin1II CATG 4 cut(s) 81, 93, 105, 156
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HpaII CCGG 1 cut(s) 257
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 3 cut(s) 104, 121, 134
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 4 cut(s) 84, 125, 196, 219
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 279, 328
HpySE526I ACGT 1 cut(s) 365
Hsp92II CATG 4 cut(s) 81, 93, 105, 156
HspAI GCGC 1 cut(s) 238
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 1 cut(s) 270
Lsp1109I GCAGC 2 cut(s) 155, 281
LweI GCATC 1 cut(s) 228
MaeI CTAG 2 cut(s) 339, 410
MaeII ACGT 1 cut(s) 365
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MhlI GDGCHC 3 cut(s) 310, 324, 333
MluCI AATT 7 cut(s) 120, 129, 197, 286, 299, 374, 383
MnlI CCTC 2 cut(s) 335, 364
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 3 cut(s) 159, 285, 442
MspI CCGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 3 cut(s) 90, 279, 328
NdeII GATC 1 cut(s) 349
NlaIII CATG 4 cut(s) 81, 93, 105, 156
NsbI TGCGCA 1 cut(s) 239
NspI RCATGY 2 cut(s) 81, 93
PciI ACATGT 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 8
PkrI GCNGC 2 cut(s) 170, 296
Ppu21I YACGTR 1 cut(s) 366
PscI ACATGT 1 cut(s) 77
PsiI TTATAA 1 cut(s) 396
Psp124BI GAGCTC 1 cut(s) 333
PspPI GGNCC 1 cut(s) 39
RsaI GTAC 2 cut(s) 364, 368
RsaNI GTAC 2 cut(s) 363, 367
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 3 cut(s) 159, 285, 442
SatI GCNGC 2 cut(s) 169, 295
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 1 cut(s) 39
SduI GDGCHC 3 cut(s) 310, 324, 333
SetI ASST 5 cut(s) 113, 268, 333, 340, 368
SfaNI GCATC 1 cut(s) 228
SfcI CTRYAG 1 cut(s) 223
SinI GGWCC 1 cut(s) 39
SnaBI TACGTA 1 cut(s) 366
Sse9I AATT 7 cut(s) 120, 129, 197, 286, 299, 374, 383
SsiI CCGC 1 cut(s) 326
SspI AATATT 1 cut(s) 7
SspMI CTAG 2 cut(s) 339, 410
SstI GAGCTC 1 cut(s) 333
StyI CCWWGG 1 cut(s) 163
TaiI ACGT 1 cut(s) 368
TasI AATT 7 cut(s) 120, 129, 197, 286, 299, 374, 383
Tru1I TTAA 3 cut(s) 159, 285, 442
Tru9I TTAA 3 cut(s) 159, 285, 442
TseI GCWGC 2 cut(s) 168, 294
TspDTI ATGAA 4 cut(s) 17, 141, 153, 387
TspGWI ACGGA 2 cut(s) 31, 192
VpaK11BI GGWCC 1 cut(s) 39
XceI RCATGY 2 cut(s) 81, 93
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 339, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.