pycom06g19070

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000960
Physical Location & Seq
Forward (+)
22585641 .. 22587253
1613 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19070.2

Sequence Viewer

Length: 1164 bp
ATGGAAGATCAACCAAATCAGGCGACGCCGCTGGTATTCCACCCTCAAACGTCAAAATTAGCGGCACTACATGTACATGTACAAAAACTCCACCAACATCACCACCAACCTCAACAACTCCCCCCTCCAAATAACCCTAATATAAATTCCCACGTCAACATTGTTAACATACGACCTAGTCCTGATCCCGACGCCGATAATCATCAGGCAAACCCCTCTTCCTCCTCCCACGAAAATGCCGGGACATCACGCTTTGATCATCAGGAATATCATGACCACGATCACGATCATCATCAACAAGCCCGACCATTACATGTAGTACAGATGCCACCCTCAAACCTCAATAGTGCTGATCTCATCTTTCACCAGCAGATTAACAATTGGCCTCACCATAATTACAACGCGCACGCGCCTTCACGTACATATTATCCTCCTCCTCATCATCACCACCGAGCTGCTTCGGCCGATGCGCTTCATCATTATCATCATCGTCACCATCGAGATGCTTCTGCTGATGCAATTTATTATGGTTCACACCAAGTGCTTTTCCCTCCTCCGGCTGCCTACCAAATTGTTCCTCATCAGCAGCTGCAGCTGCAGTATTATAATCATGCTTCTACCGCAAGAAATCAACTCCTGCCAGCGTTGCATTGTAACAGTCATTTTGCTGAAACAGGAGTACCCATCCAAAACATTGAATACGTACCAGTGCTGCTGCCTCTGCCAGGCATCGATGGAACATGGAAAACGGGGCTCTTCGAGTGCATGGAGGATCCCCCAAATGCTATCATGACGCTATTGTGCCCATGCTGGACGTTCGGTCAGATTGCAGAGATCGTCAATAATGGTCGAAGCTCTTGTGCAGCCAATGCACTGATTTACATGTTGATCATGCTGTGCATCTTTGTGCCATGCTTGCTATCGTGCACATACCGGAAAAAGCTGAGGAACAAGTTCGACCTGCCCGAAAGCCCCGCTCCGGATTGCATCACTCACTTCTTATGCGAGTGGTGTGCTCTCTGCCAAGAACATAGGGAGCTCCAGCTCAGAGGCCTAGACCCTACTTTAGGATGGGTAGGAAACTTGGAGCAAATTCAGAGGATGCAGCAAAAGCGGCAAGCTGCTATGAGTCCGCCAACGACCCAAAGAATGACGGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

388

Amino Acids

44.11

Weight (kDa)

6.61

Isoelectric Point (pI)

57.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
Acc36I ACCTGC 1 cut(s) 969
AccBSI CCGCTC 1 cut(s) 977
AccII CGCG 2 cut(s) 404, 410
AccIII TCCGGA 1 cut(s) 979
AciI CCGC 6 cut(s) 29, 62, 621, 975, 1114, 1133
AclWI GGATC 3 cut(s) 179, 767, 780
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 2 cut(s) 145, 1092
AcyI GRCGYC 2 cut(s) 26, 192
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 6 cut(s) 75, 81, 321, 421, 681, 705
AfiI CCNNNNNNNGG 4 cut(s) 556, 725, 979, 1067
AflIII ACRYGT 4 cut(s) 70, 76, 313, 882
AgsI TTSAA 1 cut(s) 698
AhdI GACNNNNNGTC 1 cut(s) 819
AjiI CACGTC 1 cut(s) 154
AjnI CCWGG 1 cut(s) 724
AluBI AGCT 8 cut(s) 455, 589, 595, 855, 943, 1039, 1045, 1121
AluI AGCT 8 cut(s) 455, 589, 595, 855, 943, 1039, 1045, 1121
Alw21I GWGCWC 3 cut(s) 929, 1018, 1041
Alw44I GTGCAC 1 cut(s) 925
AlwI GGATC 3 cut(s) 179, 767, 780
AlwNI CAGNNNCTG 1 cut(s) 589
Aor13HI TCCGGA 1 cut(s) 979
AoxI GGCC 3 cut(s) 383, 462, 1051
ApaLI GTGCAC 1 cut(s) 925
ApoI RAATTY 2 cut(s) 145, 1092
Asp700I GAANNNNTTC 1 cut(s) 953
AspLEI GCGC 3 cut(s) 406, 412, 472
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 5 cut(s) 92, 356, 380, 437, 485
BaeGI GKGCMC 2 cut(s) 806, 929
BaeI ACNNNNGTAYC 2 cut(s) 663, 696
BamHI GGATCC 1 cut(s) 772
BanII GRGCYC 2 cut(s) 756, 1041
Bbv12I GWGCWC 3 cut(s) 929, 1018, 1041
BbvCI CCTCAGC 1 cut(s) 944
BccI CCATC 4 cut(s) 504, 692, 728, 1065
BcgI CGANNNNNNTGC 2 cut(s) 995, 1029
BciT130I CCWGG 1 cut(s) 726
BclI TGATCA 2 cut(s) 256, 888
BcnI CCSGG 1 cut(s) 241
BfaI CTAG 2 cut(s) 177, 1055
BfmI CTRYAG 2 cut(s) 590, 596
BfuAI ACCTGC 1 cut(s) 969
Bme1390I CCNGG 2 cut(s) 241, 726
BmeRI GACNNNNNGTC 1 cut(s) 819
BmgBI CACGTC 1 cut(s) 154
BmiI GGNNCC 1 cut(s) 774
BmrFI CCNGG 2 cut(s) 241, 726
BmsI GCATC 8 cut(s) 315, 457, 493, 505, 738, 909, 996, 1092
BpmI CTGGAG 1 cut(s) 1025
Bpu10I CCTNAGC 1 cut(s) 944
BpuMI CCSGG 1 cut(s) 241
Bsa29I ATCGAT 1 cut(s) 732
BsaAI YACGTR 2 cut(s) 419, 703
BsaBI GATNNNNATC 3 cut(s) 201, 285, 291
BsaHI GRCGYC 2 cut(s) 26, 192
BsaWI WCCGGW 2 cut(s) 933, 979
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 4 cut(s) 556, 725, 979, 1067
Bse1I ACTGG 1 cut(s) 707
Bse8I GATNNNNATC 3 cut(s) 201, 285, 291
BseAI TCCGGA 1 cut(s) 979
BseBI CCWGG 1 cut(s) 726
BseCI ATCGAT 1 cut(s) 732
BseGI GGATG 3 cut(s) 684, 1076, 1107
BseJI GATNNNNATC 3 cut(s) 201, 285, 291
BseLI CCNNNNNNNGG 4 cut(s) 556, 725, 979, 1067
BseMII CTCAG 2 cut(s) 935, 1060
BseNI ACTGG 1 cut(s) 707
BseRI GAGGAG 4 cut(s) 214, 423, 426, 543
BseSI GKGCMC 2 cut(s) 806, 929
BseX3I CGGCCG 1 cut(s) 462
BsgI GTGCAG 1 cut(s) 882
Bsh1236I CGCG 2 cut(s) 404, 410
Bsh1285I CGRYCG 1 cut(s) 465
BshFI GGCC 3 cut(s) 385, 464, 1053
BshVI ATCGAT 1 cut(s) 732
BsiEI CGRYCG 1 cut(s) 465
BsiHKAI GWGCWC 3 cut(s) 929, 1018, 1041
BsiSI CCGG 4 cut(s) 240, 557, 934, 980
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 4 cut(s) 556, 725, 979, 1067
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 3 cut(s) 385, 464, 1053
Bsp1286I GDGCHC 5 cut(s) 756, 806, 929, 1018, 1041
Bsp13I TCCGGA 1 cut(s) 979
Bsp1407I TGTACA 2 cut(s) 73, 79
Bsp143I GATC 9 cut(s) 7, 184, 256, 280, 286, 352, 772, 834, 888
BspACI CCGC 6 cut(s) 29, 62, 621, 975, 1114, 1133
BspANI GGCC 3 cut(s) 385, 464, 1053
BspCNI CTCAG 2 cut(s) 936, 1059
BspDI ATCGAT 1 cut(s) 732
BspEI TCCGGA 1 cut(s) 979
BspFNI CGCG 2 cut(s) 404, 410
BspHI TCATGA 2 cut(s) 271, 789
BspLI GGNNCC 1 cut(s) 774
BspMAI CTGCAG 2 cut(s) 594, 600
BspMI ACCTGC 1 cut(s) 969
BspPI GGATC 3 cut(s) 179, 767, 780
BspQI GCTCTTC 1 cut(s) 761
BsrBI CCGCTC 1 cut(s) 977
BsrGI TGTACA 2 cut(s) 73, 79
BsrI ACTGG 1 cut(s) 707
BssMI GATC 9 cut(s) 7, 184, 256, 280, 286, 352, 772, 834, 888
BssNI GRCGYC 2 cut(s) 26, 192
Bst2UI CCWGG 1 cut(s) 726
Bst4CI ACNGT 1 cut(s) 659
Bst6I CTCTTC 2 cut(s) 223, 761
BstACI GRCGYC 2 cut(s) 26, 192
BstAPI GCANNNNNTGC 1 cut(s) 869
BstAUI TGTACA 2 cut(s) 73, 79
BstBAI YACGTR 2 cut(s) 419, 703
BstC8I GCNNGC 4 cut(s) 408, 642, 917, 1119
BstDEI CTNAG 2 cut(s) 944, 1046
BstENI CCTNNNNNAGG 2 cut(s) 723, 1065
BstF5I GGATG 3 cut(s) 684, 1076, 1107
BstFNI CGCG 2 cut(s) 404, 410
BstHHI GCGC 3 cut(s) 406, 412, 472
BstKTI GATC 9 cut(s) 10, 187, 259, 283, 289, 355, 775, 837, 891
BstMBI GATC 9 cut(s) 7, 184, 256, 280, 286, 352, 772, 834, 888
BstMCI CGRYCG 1 cut(s) 465
BstNI CCWGG 1 cut(s) 726
BstNSI RCATGY 4 cut(s) 74, 80, 317, 886
BstSCI CCNGG 2 cut(s) 239, 724
BstSFI CTRYAG 2 cut(s) 590, 596
BstSLI GKGCMC 2 cut(s) 806, 929
BstSNI TACGTA 1 cut(s) 703
BstUI CGCG 2 cut(s) 404, 410
BstX2I RGATCY 1 cut(s) 772
BstYI RGATCY 1 cut(s) 772
BstZI CGGCCG 1 cut(s) 462
Bsu15I ATCGAT 1 cut(s) 732
BsuRI GGCC 3 cut(s) 385, 464, 1053
BsuTUI ATCGAT 1 cut(s) 732
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 3 cut(s) 684, 1076, 1107
BtsIMutI CAGTG 2 cut(s) 714, 872
BveI ACCTGC 1 cut(s) 969
Cac8I GCNNGC 4 cut(s) 408, 642, 917, 1119
CaiI CAGNNNCTG 1 cut(s) 589
CciI TCATGA 2 cut(s) 271, 789
CfoI GCGC 3 cut(s) 406, 412, 472
ClaI ATCGAT 1 cut(s) 732
CseI GACGC 3 cut(s) 34, 200, 802
Csp6I GTAC 6 cut(s) 74, 80, 320, 420, 680, 704
CviQI GTAC 6 cut(s) 74, 80, 320, 420, 680, 704
DdeI CTNAG 2 cut(s) 944, 1046
DpnI GATC 9 cut(s) 9, 186, 258, 282, 288, 354, 774, 836, 890
DpnII GATC 9 cut(s) 7, 184, 256, 280, 286, 352, 772, 834, 888
DraIII CACNNNGTG 1 cut(s) 541
DriI GACNNNNNGTC 1 cut(s) 819
EaeI YGGCCR 1 cut(s) 462
EagI CGGCCG 1 cut(s) 462
Eam1104I CTCTTC 2 cut(s) 223, 761
Eam1105I GACNNNNNGTC 1 cut(s) 819
EarI CTCTTC 2 cut(s) 223, 761
EciI GGCGGA 1 cut(s) 1122
Ecl136II GAGCTC 1 cut(s) 1039
EclXI CGGCCG 1 cut(s) 462
Eco105I TACGTA 1 cut(s) 703
Eco147I AGGCCT 1 cut(s) 1053
Eco24I GRGCYC 2 cut(s) 756, 1041
Eco52I CGGCCG 1 cut(s) 462
Eco53kI GAGCTC 1 cut(s) 1039
EcoICRI GAGCTC 1 cut(s) 1039
EcoNI CCTNNNNNAGG 2 cut(s) 723, 1065
EcoRII CCWGG 1 cut(s) 724
EcoT38I GRGCYC 2 cut(s) 756, 1041
FaqI GGGAC 1 cut(s) 256
FauI CCCGC 1 cut(s) 982
FbaI TGATCA 2 cut(s) 256, 888
FokI GGATG 3 cut(s) 671, 1083, 1114
FriOI GRGCYC 2 cut(s) 756, 1041
FspBI CTAG 2 cut(s) 177, 1055
GlaI GCGC 3 cut(s) 405, 411, 471
GsuI CTGGAG 1 cut(s) 1025
HaeIII GGCC 3 cut(s) 385, 464, 1053
HapII CCGG 4 cut(s) 240, 557, 934, 980
HgaI GACGC 3 cut(s) 34, 200, 802
HhaI GCGC 3 cut(s) 406, 412, 472
Hin1I GRCGYC 2 cut(s) 26, 192
Hin6I GCGC 3 cut(s) 404, 410, 470
HinP1I GCGC 3 cut(s) 404, 410, 470
HincII GTYRAC 2 cut(s) 157, 166
HindII GTYRAC 2 cut(s) 157, 166
HinfI GANTC 1 cut(s) 1129
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 4 cut(s) 240, 557, 934, 980
HphI GGTGA 5 cut(s) 92, 356, 380, 437, 485
Hpy166II GTNNAC 4 cut(s) 157, 166, 533, 927
Hpy188I TCNGA 3 cut(s) 825, 1049, 1098
Hpy188III TCNNGA 8 cut(s) 182, 188, 263, 272, 284, 500, 790, 980
Hpy8I GTNNAC 4 cut(s) 157, 166, 533, 927
Hpy99I CGWCG 2 cut(s) 28, 194
HpyAV CCTTC 1 cut(s) 423
HpyCH4III ACNGT 1 cut(s) 659
HpyCH4IV ACGT 5 cut(s) 50, 153, 418, 702, 815
HpyF3I CTNAG 2 cut(s) 944, 1046
HpySE526I ACGT 5 cut(s) 50, 153, 418, 702, 815
Hsp92I GRCGYC 2 cut(s) 26, 192
HspAI GCGC 3 cut(s) 404, 410, 470
Kpn2I TCCGGA 1 cut(s) 979
Ksp22I TGATCA 2 cut(s) 256, 888
KspAI GTTAAC 1 cut(s) 166
Kzo9I GATC 9 cut(s) 7, 184, 256, 280, 286, 352, 772, 834, 888
LguI GCTCTTC 1 cut(s) 761
LmnI GCTCC 4 cut(s) 982, 1036, 1044, 1087
LweI GCATC 8 cut(s) 315, 457, 493, 505, 738, 909, 996, 1092
MaeI CTAG 2 cut(s) 177, 1055
MaeII ACGT 5 cut(s) 50, 153, 418, 702, 815
MaeIII GTNAC 2 cut(s) 491, 653
MalI GATC 9 cut(s) 9, 186, 258, 282, 288, 354, 774, 836, 890
MbiI CCGCTC 1 cut(s) 977
MboI GATC 9 cut(s) 7, 184, 256, 280, 286, 352, 772, 834, 888
MboII GAAGA 3 cut(s) 17, 210, 748
MfeI CAATTG 1 cut(s) 379
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 5 cut(s) 756, 806, 929, 1018, 1041
MluCI AATT 7 cut(s) 56, 145, 379, 394, 519, 570, 1092
MlyI GAGTC 1 cut(s) 1138
MroI TCCGGA 1 cut(s) 979
MroXI GAANNNNTTC 1 cut(s) 953
MseI TTAA 2 cut(s) 165, 375
MslI CAYNNNNRTG 4 cut(s) 75, 234, 501, 905
MspA1I CMGCKG 3 cut(s) 31, 589, 595
MspI CCGG 4 cut(s) 240, 557, 934, 980
MspR9I CCNGG 2 cut(s) 241, 726
MunI CAATTG 1 cut(s) 379
MvaI CCWGG 1 cut(s) 726
MvnI CGCG 2 cut(s) 404, 410
NciI CCSGG 1 cut(s) 241
NdeII GATC 9 cut(s) 7, 184, 256, 280, 286, 352, 772, 834, 888
NlaIV GGNNCC 1 cut(s) 774
NmuCI GTSAC 1 cut(s) 491
NspI RCATGY 4 cut(s) 74, 80, 317, 886
PagI TCATGA 2 cut(s) 271, 789
PceI AGGCCT 1 cut(s) 1053
PciI ACATGT 4 cut(s) 70, 76, 313, 882
PciSI GCTCTTC 1 cut(s) 761
PcsI WCGNNNNNNNCGW 2 cut(s) 496, 963
PdmI GAANNNNTTC 1 cut(s) 953
PflFI GACNNNGTC 1 cut(s) 177
PleI GAGTC 1 cut(s) 1137
PpsI GAGTC 1 cut(s) 1137
Ppu21I YACGTR 2 cut(s) 419, 703
PscI ACATGT 4 cut(s) 70, 76, 313, 882
PsiI TTATAA 1 cut(s) 606
Psp124BI GAGCTC 1 cut(s) 1041
Psp6I CCWGG 1 cut(s) 724
PspGI CCWGG 1 cut(s) 724
PspN4I GGNNCC 1 cut(s) 774
PstI CTGCAG 2 cut(s) 594, 600
PstNI CAGNNNCTG 1 cut(s) 589
PsuI RGATCY 1 cut(s) 772
PsyI GACNNNGTC 1 cut(s) 177
PvuII CAGCTG 2 cut(s) 589, 595
RsaI GTAC 6 cut(s) 75, 81, 321, 421, 681, 705
RsaNI GTAC 6 cut(s) 74, 80, 320, 420, 680, 704
RseI CAYNNNNRTG 4 cut(s) 75, 234, 501, 905
SacI GAGCTC 1 cut(s) 1041
SapI GCTCTTC 1 cut(s) 761
SaqAI TTAA 2 cut(s) 165, 375
Sau3AI GATC 9 cut(s) 7, 184, 256, 280, 286, 352, 772, 834, 888
SchI GAGTC 1 cut(s) 1138
ScrFI CCNGG 2 cut(s) 241, 726
SduI GDGCHC 5 cut(s) 756, 806, 929, 1018, 1041
SfaNI GCATC 8 cut(s) 315, 457, 493, 505, 738, 909, 996, 1092
SfcI CTRYAG 2 cut(s) 590, 596
SmiMI CAYNNNNRTG 4 cut(s) 75, 234, 501, 905
SnaBI TACGTA 1 cut(s) 703
Sse9I AATT 7 cut(s) 56, 145, 379, 394, 519, 570, 1092
SseBI AGGCCT 1 cut(s) 1053
SsiI CCGC 6 cut(s) 29, 62, 621, 975, 1114, 1133
SspMI CTAG 2 cut(s) 177, 1055
SstI GAGCTC 1 cut(s) 1041
StuI AGGCCT 1 cut(s) 1053
StyD4I CCNGG 2 cut(s) 239, 724
TaaI ACNGT 1 cut(s) 659
TaiI ACGT 5 cut(s) 53, 156, 421, 705, 818
TaqI TCGA 5 cut(s) 499, 732, 759, 850, 957
TaqII GACCGA 1 cut(s) 809
TasI AATT 7 cut(s) 56, 145, 379, 394, 519, 570, 1092
TatI WGTACW 3 cut(s) 73, 79, 319
TauI GCSGC 3 cut(s) 31, 65, 1117
Tru1I TTAA 2 cut(s) 165, 375
Tru9I TTAA 2 cut(s) 165, 375
TscAI CASTG 2 cut(s) 714, 879
TseFI GTSAC 1 cut(s) 491
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 1 cut(s) 464
TspRI CASTG 2 cut(s) 714, 879
Tth111I GACNNNGTC 1 cut(s) 177
VneI GTGCAC 1 cut(s) 925
XagI CCTNNNNNAGG 2 cut(s) 723, 1065
XapI RAATTY 2 cut(s) 145, 1092
XceI RCATGY 4 cut(s) 74, 80, 317, 886
XmnI GAANNNNTTC 1 cut(s) 953
XspI CTAG 2 cut(s) 177, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.