FvH4_5g12460

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000960
Physical Location & Seq
Reverse (-)
7022464 .. 7024561
2098 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12460.t2

Sequence Viewer

Length: 1203 bp
ATGGAGGAGGATCATCATACTCCGGATTACGAAGACCACTACCATGGTGATCAACCTGAAGCTCATGTAGAGGTACAAGCCTCAGATATTAGTGACGCTGCTGCAAACTACGACCAAAACCAACAGCAACAACAGCAGCAGCCACCTCAATATCAGCCACAACCTCCACCACAAGGAGGCCAACAACAACCGCAGTTCTTCCAGCAGCAGCAGCAGCCACAGCTGCGTCAGCCACCTGTACAATTCCCACCACAACAACCACCACAAGGTCCACCGCGGCCAATCCAATACTTGGCTCCACAGCAGCCTCGTCCCCCTCCACAACAGTTCCCACCACAAAGTCCTCCTCAGTTTCAAGCTCAGCAGCCTCCTTATAAGCCACCTCAACAGTTTCCACCACAGAATGGCACGCCAAACCAACAGAACCACCCTTTTGGACAACCACCTCCTCCGGCCAAATATCCTCCCCAACCACCTTCTCCGGTAAAATATCCTCCACAATCTCCGGCGCAATATCCTCCTCCTGCAGGAGGTTCATCTCAGCCTCCTCAAGGCTACAACGGCATCCCCCAACCTGCAAAGCAGAATCAGACGCCACCTCCGGTGCAAGTTGGCTATAATAAGCCGCAGCAGCCACCCCAACAGTACTACCAGCAGCAACAATACCAACCACAGCAGAATCATTATGCGGCTCCTCCTGCTCCGGCTCAAGGAGTTCCAATGCAGGGCCCTTACGGTCCGCAGGGAACACCTATGACTATGAATATGATGAACCCGATTGGCACTGAAGGTTGGAAAACTGGGCTCTTTGACTGCATGGATGATCCTAATAATGCTCTCATAACGTTATTCGTACCATGCTTGACATTCGGCCAGATAGCAGAAGTTGTGGATAACGGCACTACCTCATGTGGTACGAGTGCATTGCTATACGGCTTGGTTGGAGCCTTCATCGGGGTGCCGTTCATAATGTCATGCACCTACCGGACGAAGATTCGGAGCAAGTATGGGCTAGTTGAGACCCCTGCACCTGACTGGGTTACCCATTTGTTTTGTGAGCCATGTTCTCTTTGCCAAGAGTACAGAGAACTTAATATGAGAGGGCTAGACCCTTCTATAGGATGGCAAGGAAATGTGGCAAGGGCCATGCAGCAGCAACAACCTAATATGATGACGCCTCCAATGAACCAAATGATGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

401

Amino Acids

44.66

Weight (kDa)

5.55

Isoelectric Point (pI)

78.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 265 - 302 4.8e-09 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
Acc36I ACCTGC 1 cut(s) 583
AccB1I GGYRCC 1 cut(s) 958
AccB7I CCANNNNNTGG 2 cut(s) 292, 404
AccII CGCG 1 cut(s) 277
AccIII TCCGGA 1 cut(s) 22
AciI CCGC 6 cut(s) 191, 275, 277, 626, 689, 740
AclI AACGTT 1 cut(s) 845
AclWI GGATC 2 cut(s) 18, 818
AcoI YGGCCR 3 cut(s) 278, 453, 871
AcuI CTGAAG 2 cut(s) 78, 807
AcyI GRCGYC 2 cut(s) 593, 1175
AfaI GTAC 6 cut(s) 75, 240, 647, 855, 916, 1082
AgsI TTSAA 1 cut(s) 356
AluBI AGCT 3 cut(s) 62, 223, 359
AluI AGCT 3 cut(s) 62, 223, 359
Alw26I GTCTC 1 cut(s) 1013
AlwI GGATC 2 cut(s) 18, 818
Aor13HI TCCGGA 1 cut(s) 22
AoxI GGCC 6 cut(s) 178, 278, 453, 727, 871, 1143
ApaI GGGCCC 1 cut(s) 731
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 5 cut(s) 269, 727, 728, 737, 1143
AsuHPI GGTGA 1 cut(s) 59
AvaII GGWCC 2 cut(s) 269, 737
BaeGI GKGCMC 1 cut(s) 731
BanI GGYRCC 1 cut(s) 958
BanII GRGCYC 2 cut(s) 731, 807
BbsI GAAGAC 1 cut(s) 39
BccI CCATC 1 cut(s) 1116
BceAI ACGGC 4 cut(s) 577, 913, 946, 949
BcgI CGANNNNNNTGC 2 cut(s) 907, 941
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 1013
BfaI CTAG 2 cut(s) 1013, 1106
BfmI CTRYAG 2 cut(s) 525, 1116
BfuAI ACCTGC 1 cut(s) 583
BlpI GCTNAGC 1 cut(s) 360
BmcAI AGTACT 1 cut(s) 647
Bme18I GGWCC 2 cut(s) 269, 737
BmgT120I GGNCC 5 cut(s) 269, 727, 728, 737, 1143
BmiI GGNNCC 5 cut(s) 297, 693, 729, 946, 960
BmrI ACTGGG 2 cut(s) 810, 1045
BmsI GCATC 1 cut(s) 573
BmuI ACTGGG 2 cut(s) 810, 1045
BpiI GAAGAC 1 cut(s) 39
Bpu1102I GCTNAGC 1 cut(s) 360
BpuEI CTTGAG 2 cut(s) 534, 693
BsaHI GRCGYC 2 cut(s) 593, 1175
BsaI GGTCTC 1 cut(s) 1013
BsaJI CCNNGG 2 cut(s) 43, 275
BsaWI WCCGGW 4 cut(s) 22, 481, 601, 984
BsaXI ACNNNNNCTCC 2 cut(s) 583, 613
Bse1I ACTGG 2 cut(s) 805, 1040
Bse3DI GCAATG 1 cut(s) 923
BseAI TCCGGA 1 cut(s) 22
BseDI CCNNGG 2 cut(s) 43, 275
BseGI GGATG 3 cut(s) 564, 826, 1127
BseMI GCAATG 1 cut(s) 923
BseMII CTCAG 4 cut(s) 96, 362, 374, 554
BseNI ACTGG 2 cut(s) 805, 1040
BseRI GAGGAG 6 cut(s) 20, 336, 438, 510, 537, 684
BseSI GKGCMC 1 cut(s) 731
BsgI GTGCAG 1 cut(s) 1011
Bsh1236I CGCG 1 cut(s) 277
BshFI GGCC 6 cut(s) 180, 280, 455, 729, 873, 1145
BshNI GGYRCC 1 cut(s) 958
BsiSI CCGG 7 cut(s) 23, 452, 482, 506, 602, 704, 985
BslFI GGGAC 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 1013
BsmFI GGGAC 1 cut(s) 297
BsnI GGCC 6 cut(s) 180, 280, 455, 729, 873, 1145
Bso31I GGTCTC 1 cut(s) 1013
Bsp120I GGGCCC 1 cut(s) 727
Bsp1286I GDGCHC 2 cut(s) 731, 807
Bsp13I TCCGGA 1 cut(s) 22
Bsp1407I TGTACA 1 cut(s) 238
Bsp143I GATC 3 cut(s) 10, 49, 823
Bsp1720I GCTNAGC 1 cut(s) 360
Bsp19I CCATGG 1 cut(s) 43
BspACI CCGC 6 cut(s) 191, 275, 277, 626, 689, 740
BspANI GGCC 6 cut(s) 180, 280, 455, 729, 873, 1145
BspCNI CTCAG 4 cut(s) 95, 361, 373, 553
BspEI TCCGGA 1 cut(s) 22
BspFNI CGCG 1 cut(s) 277
BspLI GGNNCC 5 cut(s) 297, 693, 729, 946, 960
BspMAI CTGCAG 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 2 cut(s) 18, 818
BspT107I GGYRCC 1 cut(s) 958
BspTNI GGTCTC 1 cut(s) 1013
BsrDI GCAATG 1 cut(s) 923
BsrGI TGTACA 1 cut(s) 238
BsrI ACTGG 2 cut(s) 805, 1040
BssECI CCNNGG 2 cut(s) 43, 275
BssMI GATC 3 cut(s) 10, 49, 823
BssNI GRCGYC 2 cut(s) 593, 1175
BssT1I CCWWGG 1 cut(s) 43
Bst4CI ACNGT 4 cut(s) 327, 390, 645, 737
BstACI GRCGYC 2 cut(s) 593, 1175
BstAUI TGTACA 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 4 cut(s) 82, 348, 360, 540
BstDSI CCRYGG 2 cut(s) 43, 275
BstEII GGTNACC 1 cut(s) 1039
BstENI CCTNNNNNAGG 4 cut(s) 525, 528, 549, 1116
BstF5I GGATG 3 cut(s) 564, 826, 1127
BstFNI CGCG 1 cut(s) 277
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 3 cut(s) 13, 52, 826
BstMAI GTCTC 1 cut(s) 1013
BstMBI GATC 3 cut(s) 10, 49, 823
BstMWI GCNNNNNNNGC 9 cut(s) 133, 211, 214, 220, 223, 229, 561, 631, 698
BstPI GGTNACC 1 cut(s) 1039
BstSFI CTRYAG 2 cut(s) 525, 1116
BstSLI GKGCMC 1 cut(s) 731
BstUI CGCG 1 cut(s) 277
BstV2I GAAGAC 1 cut(s) 39
BstXI CCANNNNNNTGG 2 cut(s) 44, 434
BsuRI GGCC 6 cut(s) 180, 280, 455, 729, 873, 1145
BtgI CCRYGG 2 cut(s) 43, 275
BtsCI GGATG 3 cut(s) 564, 826, 1127
BtsIMutI CAGTG 1 cut(s) 783
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 1 cut(s) 410
CfoI GCGC 1 cut(s) 511
Cfr13I GGNCC 5 cut(s) 269, 727, 728, 737, 1143
Cfr42I CCGCGG 1 cut(s) 278
CpoI CGGWCCG 1 cut(s) 737
CseI GACGC 4 cut(s) 104, 215, 601, 1183
Csp6I GTAC 6 cut(s) 74, 239, 646, 854, 915, 1081
CspI CGGWCCG 1 cut(s) 737
CviAII CATG 8 cut(s) 44, 65, 817, 858, 909, 975, 1062, 1147
CviQI GTAC 6 cut(s) 74, 239, 646, 854, 915, 1081
DdeI CTNAG 4 cut(s) 82, 348, 360, 540
DpnI GATC 3 cut(s) 12, 51, 825
DpnII GATC 3 cut(s) 10, 49, 823
EaeI YGGCCR 3 cut(s) 278, 453, 871
Eco130I CCWWGG 1 cut(s) 43
Eco24I GRGCYC 2 cut(s) 731, 807
Eco31I GGTCTC 1 cut(s) 1013
Eco47I GGWCC 2 cut(s) 269, 737
Eco57I CTGAAG 2 cut(s) 78, 807
Eco91I GGTNACC 1 cut(s) 1039
EcoNI CCTNNNNNAGG 4 cut(s) 525, 528, 549, 1116
EcoO109I RGGNCCY 2 cut(s) 727, 728
EcoO65I GGTNACC 1 cut(s) 1039
EcoT14I CCWWGG 1 cut(s) 43
EcoT38I GRGCYC 2 cut(s) 731, 807
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 8 cut(s) 47, 68, 820, 861, 912, 978, 1065, 1150
FaqI GGGAC 1 cut(s) 297
FatI CATG 8 cut(s) 43, 64, 816, 857, 908, 974, 1061, 1146
FbaI TGATCA 1 cut(s) 49
FokI GGATG 3 cut(s) 551, 833, 1134
FriOI GRGCYC 2 cut(s) 731, 807
FspBI CTAG 2 cut(s) 1013, 1106
GlaI GCGC 1 cut(s) 510
HaeIII GGCC 6 cut(s) 180, 280, 455, 729, 873, 1145
HapII CCGG 7 cut(s) 23, 452, 482, 506, 602, 704, 985
HgaI GACGC 4 cut(s) 104, 215, 601, 1183
HhaI GCGC 1 cut(s) 511
Hin1I GRCGYC 2 cut(s) 593, 1175
Hin1II CATG 8 cut(s) 47, 68, 820, 861, 912, 978, 1065, 1150
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HinfI GANTC 3 cut(s) 586, 679, 994
HpaII CCGG 7 cut(s) 23, 452, 482, 506, 602, 704, 985
HphI GGTGA 1 cut(s) 59
Hpy166II GTNNAC 1 cut(s) 272
Hpy188I TCNGA 3 cut(s) 85, 591, 999
Hpy188III TCNNGA 1 cut(s) 23
Hpy8I GTNNAC 1 cut(s) 272
HpyAV CCTTC 4 cut(s) 486, 782, 958, 1122
HpyCH4III ACNGT 4 cut(s) 327, 390, 645, 737
HpyCH4IV ACGT 1 cut(s) 845
HpyF10VI GCNNNNNNNGC 9 cut(s) 133, 211, 214, 220, 223, 229, 561, 631, 698
HpyF3I CTNAG 4 cut(s) 82, 348, 360, 540
HpySE526I ACGT 1 cut(s) 845
Hsp92I GRCGYC 2 cut(s) 593, 1175
Hsp92II CATG 8 cut(s) 47, 68, 820, 861, 912, 978, 1065, 1150
HspAI GCGC 1 cut(s) 509
Kpn2I TCCGGA 1 cut(s) 22
Ksp22I TGATCA 1 cut(s) 49
KspI CCGCGG 1 cut(s) 278
Kzo9I GATC 3 cut(s) 10, 49, 823
LmnI GCTCC 5 cut(s) 301, 697, 706, 944, 999
LweI GCATC 1 cut(s) 573
MaeI CTAG 2 cut(s) 1013, 1106
MaeII ACGT 1 cut(s) 845
MaeIII GTNAC 2 cut(s) 92, 1039
MalI GATC 3 cut(s) 12, 51, 825
MboI GATC 3 cut(s) 10, 49, 823
MboII GAAGA 3 cut(s) 44, 190, 1003
MhlI GDGCHC 2 cut(s) 731, 807
MluCI AATT 1 cut(s) 242
MmeI TCCRAC 2 cut(s) 773, 922
MroI TCCGGA 1 cut(s) 22
MseI TTAA 1 cut(s) 1092
MslI CAYNNNNRTG 2 cut(s) 42, 956
MspA1I CMGCKG 2 cut(s) 223, 277
MspI CCGG 7 cut(s) 23, 452, 482, 506, 602, 704, 985
MvnI CGCG 1 cut(s) 277
MwoI GCNNNNNNNGC 9 cut(s) 133, 211, 214, 220, 223, 229, 561, 631, 698
NcoI CCATGG 1 cut(s) 43
NdeII GATC 3 cut(s) 10, 49, 823
NlaIII CATG 8 cut(s) 47, 68, 820, 861, 912, 978, 1065, 1150
NlaIV GGNNCC 5 cut(s) 297, 693, 729, 946, 960
NmuCI GTSAC 1 cut(s) 92
PfeI GAWTC 3 cut(s) 586, 679, 994
PflMI CCANNNNNTGG 2 cut(s) 292, 404
PsiI TTATAA 1 cut(s) 375
Psp1406I AACGTT 1 cut(s) 845
PspEI GGTNACC 1 cut(s) 1039
PspN4I GGNNCC 5 cut(s) 297, 693, 729, 946, 960
PspOMI GGGCCC 1 cut(s) 727
PspPI GGNCC 5 cut(s) 269, 727, 728, 737, 1143
PstI CTGCAG 1 cut(s) 529
PvuII CAGCTG 1 cut(s) 223
RsaI GTAC 6 cut(s) 75, 240, 647, 855, 916, 1082
RsaNI GTAC 6 cut(s) 74, 239, 646, 854, 915, 1081
RseI CAYNNNNRTG 2 cut(s) 42, 956
Rsr2I CGGWCCG 1 cut(s) 737
RsrII CGGWCCG 1 cut(s) 737
SacII CCGCGG 1 cut(s) 278
SaqAI TTAA 1 cut(s) 1092
Sau3AI GATC 3 cut(s) 10, 49, 823
Sau96I GGNCC 5 cut(s) 269, 727, 728, 737, 1143
SbfI CCTGCAGG 1 cut(s) 529
ScaI AGTACT 1 cut(s) 647
SdaI CCTGCAGG 1 cut(s) 529
SduI GDGCHC 2 cut(s) 731, 807
SfaNI GCATC 1 cut(s) 573
SfcI CTRYAG 2 cut(s) 525, 1116
Sfr303I CCGCGG 1 cut(s) 278
SgrBI CCGCGG 1 cut(s) 278
SinI GGWCC 2 cut(s) 269, 737
SmiMI CAYNNNNRTG 2 cut(s) 42, 956
SmlI CTYRAG 2 cut(s) 549, 708
SmoI CTYRAG 2 cut(s) 549, 708
Sse8387I CCTGCAGG 1 cut(s) 529
Sse9I AATT 1 cut(s) 242
SsiI CCGC 6 cut(s) 191, 275, 277, 626, 689, 740
SspMI CTAG 2 cut(s) 1013, 1106
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 4 cut(s) 327, 390, 645, 737
TaiI ACGT 1 cut(s) 848
TasI AATT 1 cut(s) 242
TatI WGTACW 3 cut(s) 238, 645, 1080
TauI GCSGC 3 cut(s) 280, 628, 692
TfiI GAWTC 3 cut(s) 586, 679, 994
Tru1I TTAA 1 cut(s) 1092
Tru9I TTAA 1 cut(s) 1092
TscAI CASTG 1 cut(s) 790
TseFI GTSAC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 6 cut(s) 525, 776, 785, 940, 955, 1199
TspRI CASTG 1 cut(s) 790
Van91I CCANNNNNTGG 2 cut(s) 292, 404
VpaK11BI GGWCC 2 cut(s) 269, 737
XagI CCTNNNNNAGG 4 cut(s) 525, 528, 549, 1116
XspI CTAG 2 cut(s) 1013, 1106
ZrmI AGTACT 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.