Rh7DG071000

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000960
Physical Location & Seq
Forward (+)
5454991 .. 5456729
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG071000.1

Sequence Viewer

Length: 1320 bp
ATGGAGGATGATCATCACACTCCAAATTACGAAGACCACCCCCACGATAATCAACCTGAAGCTCATGTGGAGGTACAAGTCGAACAATATCAATATCAACAAGTAGCGCTTGAAATTAGCGACAATAGTGGAAACTACGACCAAAACCAGCAGCAGCAGCAGCAACAACAACAGCAGCAGCCACCTCAGTATCAGCCACAACCTCCACCACAAGGAGGCCAACAACAACAACAGTTCTTCCAGCAGCAGCAGCCGCCGCCGCCGCGTCAACCATCTCATCAGCCGCCTGTACAATTCCCACCACAACAACCACCACCGCGGCCGATCCAATACTTGGCTCCACAGCCGCCTCGCCCTGTGCCATTCCCTCTTCAGAGCCCACAAAACCAAAAGTACGCAAACCAGCCTCCTCCTCCAGGACCTACACAACAGTTCCCACCGCAAAACCCTCAGTTTCAAGCTCAACAGCCGCCTTATCAGCCACAGCCACCTCAACAGTTTCCACCACAGAATGCGACCCCTAACCAACAAAACAACCCTTTTGGACAACCACCTTCTCCGGCAAAATATCCTCCGCAACCACCTTCTCCGGTGAAATATCCTCCACAACCACCATCTCCGGTACAATATCCTCCTGCAGCAGGTTCGTCTCAGCCTCCTCAAGGCTACAATGACATTCCCCAACCTTCAAAGCAGCATCAAGCGCCGCCTCCGGTGCAAGTGGGCTATAATAAGCCTCAGCCACCCCAACAGTACCACCAGCAGCAACAATACCAACCACAACCACCACAGCAGAATCATTATGCAGCTGCTCCTTCTCCGGCTCAAGGAGTTCCGATGCAGGCCCCTTACGGGCCGCAGGGAACGCCTATGACTATGAATATGATGAACCCAATTGGCACTGAGGCTTGGAAAACCGGGCTCTTTGATTGCATGGATGATCCTAATAATGCTCTCATAACGTTATTCGTACCATGCTTGACTTTCGGCCAGATAGCAGAAGTTGTGGATAATGGCACTACCTCATGTGGTACGAGCGCATTGCTGTACGGCTTGGTTGGAGCGTTCATCGGAGTGCCATTCATAATGTCATGCACTTATCGCACGAAGATTCGGAGCAAGTATGGGCTAGTTGAGACCCCTGCACCTGACTGGGTCACCCATTTGTTTTGCGAGCCATGTTCTCTTTGCCAAGAGTACAGAGAACTTAATTTGAGAGGCCTAGACCCTTCTATAGGATGGCAAGGAAATGTGGCAAGGGCCATGCAGCAGCAACAACCTAAGATGATGACGCCTCCAGTGAACCAAATGATGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

439

Amino Acids

49.28

Weight (kDa)

5.76

Isoelectric Point (pI)

82.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 304 - 402 3.1e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 632
AccB7I CCANNNNNTGG 1 cut(s) 334
AccII CGCG 2 cut(s) 265, 319
AclI AACGTT 1 cut(s) 962
AclWI GGATC 2 cut(s) 319, 935
AcoI YGGCCR 2 cut(s) 320, 988
AcuI CTGAAG 2 cut(s) 78, 356
AcyI GRCGYC 1 cut(s) 1292
AfaI GTAC 9 cut(s) 75, 291, 395, 624, 755, 972, 1033, 1049, 1199
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 9 cut(s) 212, 215, 334, 416, 641, 662, 827, 852, 1235
AgsI TTSAA 3 cut(s) 113, 458, 690
AjnI CCWGG 1 cut(s) 415
AluBI AGCT 3 cut(s) 62, 461, 809
AluI AGCT 3 cut(s) 62, 461, 809
Alw26I GTCTC 2 cut(s) 654, 1130
AlwI GGATC 2 cut(s) 319, 935
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 7 cut(s) 217, 320, 843, 854, 988, 1219, 1260
AspLEI GCGC 3 cut(s) 109, 706, 1040
AspS9I GGNCC 4 cut(s) 419, 844, 854, 1260
AsuC2I CCSGG 1 cut(s) 919
AsuHPI GGTGA 2 cut(s) 604, 1150
AvaII GGWCC 1 cut(s) 419
BaeI ACNNNNGTAYC 2 cut(s) 173, 206
BanII GRGCYC 2 cut(s) 380, 924
BbsI GAAGAC 1 cut(s) 39
BbvCI CCTCAGC 1 cut(s) 738
BccI CCATC 3 cut(s) 280, 622, 1233
BceAI ACGGC 1 cut(s) 1066
BcgI CGANNNNNNTGC 4 cut(s) 627, 661, 1024, 1058
BciT130I CCWGG 1 cut(s) 417
BclI TGATCA 1 cut(s) 10
BcnI CCSGG 1 cut(s) 919
BcoDI GTCTC 2 cut(s) 654, 1130
BfaI CTAG 2 cut(s) 1130, 1223
BfmI CTRYAG 2 cut(s) 636, 1233
BfoI RGCGCY 2 cut(s) 110, 707
BfuAI ACCTGC 1 cut(s) 632
Bme1390I CCNGG 2 cut(s) 417, 919
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 4 cut(s) 419, 844, 854, 1260
BmiI GGNNCC 2 cut(s) 339, 846
BmrFI CCNGG 2 cut(s) 417, 919
BmrI ACTGGG 1 cut(s) 1162
BmsI GCATC 2 cut(s) 706, 828
BmuI ACTGGG 1 cut(s) 1162
BpiI GAAGAC 1 cut(s) 39
BpmI CTGGAG 2 cut(s) 399, 1281
Bpu10I CCTNAGC 1 cut(s) 738
BpuEI CTTGAG 2 cut(s) 645, 810
BpuMI CCSGG 1 cut(s) 919
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 1292
BsaI GGTCTC 1 cut(s) 1130
BsaJI CCNNGG 1 cut(s) 317
BsaWI WCCGGW 3 cut(s) 589, 619, 712
Bsc4I CCNNNNNNNGG 9 cut(s) 212, 215, 334, 416, 641, 662, 827, 852, 1235
Bse1I ACTGG 2 cut(s) 1157, 1298
Bse3DI GCAATG 1 cut(s) 1040
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 317
BseGI GGATG 3 cut(s) 13, 943, 1244
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 9 cut(s) 212, 215, 334, 416, 641, 662, 827, 852, 1235
BseMI GCAATG 1 cut(s) 1040
BseMII CTCAG 5 cut(s) 200, 464, 665, 752, 894
BseNI ACTGG 2 cut(s) 1157, 1298
BseRI GAGGAG 3 cut(s) 399, 402, 648
BseX3I CGGCCG 1 cut(s) 320
BsgI GTGCAG 1 cut(s) 1128
Bsh1236I CGCG 2 cut(s) 265, 319
Bsh1285I CGRYCG 1 cut(s) 323
BshFI GGCC 7 cut(s) 219, 322, 845, 856, 990, 1221, 1262
BsiEI CGRYCG 1 cut(s) 323
BsiSI CCGG 6 cut(s) 560, 590, 620, 713, 821, 918
BslI CCNNNNNNNGG 9 cut(s) 212, 215, 334, 416, 641, 662, 827, 852, 1235
BsmAI GTCTC 2 cut(s) 654, 1130
BsmBI CGTCTC 1 cut(s) 654
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 7 cut(s) 219, 322, 845, 856, 990, 1221, 1262
Bso31I GGTCTC 1 cut(s) 1130
Bsp1286I GDGCHC 2 cut(s) 380, 924
Bsp1407I TGTACA 1 cut(s) 289
Bsp143I GATC 3 cut(s) 10, 324, 940
BspANI GGCC 7 cut(s) 219, 322, 845, 856, 990, 1221, 1262
BspCNI CTCAG 5 cut(s) 199, 463, 664, 751, 895
BspFNI CGCG 2 cut(s) 265, 319
BspLI GGNNCC 2 cut(s) 339, 846
BspMAI CTGCAG 1 cut(s) 640
BspMI ACCTGC 1 cut(s) 632
BspPI GGATC 2 cut(s) 319, 935
BspTNI GGTCTC 1 cut(s) 1130
BsrDI GCAATG 1 cut(s) 1040
BsrGI TGTACA 1 cut(s) 289
BsrI ACTGG 2 cut(s) 1157, 1298
BssECI CCNNGG 1 cut(s) 317
BssMI GATC 3 cut(s) 10, 324, 940
BssNI GRCGYC 1 cut(s) 1292
Bst2UI CCWGG 1 cut(s) 417
Bst4CI ACNGT 4 cut(s) 234, 432, 498, 753
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 1 cut(s) 1292
BstAUI TGTACA 1 cut(s) 289
BstC8I GCNNGC 2 cut(s) 843, 1175
BstDEI CTNAG 6 cut(s) 186, 450, 651, 738, 903, 1281
BstDSI CCRYGG 1 cut(s) 317
BstEII GGTNACC 1 cut(s) 1156
BstENI CCTNNNNNAGG 4 cut(s) 414, 639, 660, 1233
BstF5I GGATG 3 cut(s) 13, 943, 1244
BstFNI CGCG 2 cut(s) 265, 319
BstH2I RGCGCY 2 cut(s) 110, 707
BstHHI GCGC 3 cut(s) 109, 706, 1040
BstKTI GATC 3 cut(s) 13, 327, 943
BstMAI GTCTC 2 cut(s) 654, 1130
BstMBI GATC 3 cut(s) 10, 324, 940
BstMCI CGRYCG 1 cut(s) 323
BstNI CCWGG 1 cut(s) 417
BstPI GGTNACC 1 cut(s) 1156
BstSCI CCNGG 2 cut(s) 415, 917
BstSFI CTRYAG 2 cut(s) 636, 1233
BstUI CGCG 2 cut(s) 265, 319
BstV2I GAAGAC 1 cut(s) 39
BstZI CGGCCG 1 cut(s) 320
BsuRI GGCC 7 cut(s) 219, 322, 845, 856, 990, 1221, 1262
BtgI CCRYGG 1 cut(s) 317
BtsCI GGATG 3 cut(s) 13, 943, 1244
BtsIMutI CAGTG 2 cut(s) 900, 1305
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 2 cut(s) 843, 1175
CfoI GCGC 3 cut(s) 109, 706, 1040
Cfr13I GGNCC 4 cut(s) 419, 844, 854, 1260
Cfr42I CCGCGG 1 cut(s) 320
CseI GACGC 2 cut(s) 254, 1300
Csp6I GTAC 9 cut(s) 74, 290, 394, 623, 754, 971, 1032, 1048, 1198
CviAII CATG 7 cut(s) 65, 934, 975, 1026, 1092, 1179, 1264
CviQI GTAC 9 cut(s) 74, 290, 394, 623, 754, 971, 1032, 1048, 1198
DdeI CTNAG 6 cut(s) 186, 450, 651, 738, 903, 1281
DpnI GATC 3 cut(s) 12, 326, 942
DpnII GATC 3 cut(s) 10, 324, 940
EaeI YGGCCR 2 cut(s) 320, 988
EagI CGGCCG 1 cut(s) 320
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
EclXI CGGCCG 1 cut(s) 320
Eco147I AGGCCT 1 cut(s) 1221
Eco24I GRGCYC 2 cut(s) 380, 924
Eco31I GGTCTC 1 cut(s) 1130
Eco47I GGWCC 1 cut(s) 419
Eco47III AGCGCT 1 cut(s) 108
Eco52I CGGCCG 1 cut(s) 320
Eco57I CTGAAG 2 cut(s) 78, 356
Eco91I GGTNACC 1 cut(s) 1156
EcoNI CCTNNNNNAGG 4 cut(s) 414, 639, 660, 1233
EcoO109I RGGNCCY 2 cut(s) 419, 844
EcoO65I GGTNACC 1 cut(s) 1156
EcoRII CCWGG 1 cut(s) 415
EcoT38I GRGCYC 2 cut(s) 380, 924
Esp3I CGTCTC 1 cut(s) 654
FaeI CATG 7 cut(s) 68, 937, 978, 1029, 1095, 1182, 1267
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FatI CATG 7 cut(s) 64, 933, 974, 1025, 1091, 1178, 1263
FbaI TGATCA 1 cut(s) 10
FokI GGATG 3 cut(s) 20, 950, 1251
FriOI GRGCYC 2 cut(s) 380, 924
FspBI CTAG 2 cut(s) 1130, 1223
GlaI GCGC 3 cut(s) 108, 705, 1039
GsuI CTGGAG 2 cut(s) 399, 1281
HaeII RGCGCY 2 cut(s) 110, 707
HaeIII GGCC 7 cut(s) 219, 322, 845, 856, 990, 1221, 1262
HapII CCGG 6 cut(s) 560, 590, 620, 713, 821, 918
HgaI GACGC 2 cut(s) 254, 1300
HhaI GCGC 3 cut(s) 109, 706, 1040
Hin1I GRCGYC 1 cut(s) 1292
Hin1II CATG 7 cut(s) 68, 937, 978, 1029, 1095, 1182, 1267
Hin6I GCGC 3 cut(s) 107, 704, 1038
HinP1I GCGC 3 cut(s) 107, 704, 1038
HincII GTYRAC 1 cut(s) 269
HindII GTYRAC 1 cut(s) 269
HinfI GANTC 2 cut(s) 796, 1111
HpaII CCGG 6 cut(s) 560, 590, 620, 713, 821, 918
HphI GGTGA 2 cut(s) 604, 1150
Hpy166II GTNNAC 2 cut(s) 269, 1303
Hpy188I TCNGA 4 cut(s) 375, 837, 1073, 1116
Hpy8I GTNNAC 2 cut(s) 269, 1303
HpyAV CCTTC 5 cut(s) 564, 594, 696, 825, 1239
HpyCH4III ACNGT 4 cut(s) 234, 432, 498, 753
HpyCH4IV ACGT 1 cut(s) 962
HpyCH4V TGCA 8 cut(s) 638, 718, 806, 841, 933, 1095, 1145, 1267
HpyF3I CTNAG 6 cut(s) 186, 450, 651, 738, 903, 1281
HpySE526I ACGT 1 cut(s) 962
Hsp92I GRCGYC 1 cut(s) 1292
Hsp92II CATG 7 cut(s) 68, 937, 978, 1029, 1095, 1182, 1267
HspAI GCGC 3 cut(s) 107, 704, 1038
Ksp22I TGATCA 1 cut(s) 10
KspI CCGCGG 1 cut(s) 320
Kzo9I GATC 3 cut(s) 10, 324, 940
LmnI GCTCC 4 cut(s) 343, 817, 1061, 1116
LweI GCATC 2 cut(s) 706, 828
MaeI CTAG 2 cut(s) 1130, 1223
MaeII ACGT 1 cut(s) 962
MaeIII GTNAC 1 cut(s) 1156
MalI GATC 3 cut(s) 12, 326, 942
MboI GATC 3 cut(s) 10, 324, 940
MboII GAAGA 4 cut(s) 44, 229, 362, 1120
MfeI CAATTG 1 cut(s) 894
MhlI GDGCHC 2 cut(s) 380, 924
MluCI AATT 5 cut(s) 25, 114, 293, 894, 1210
MmeI TCCRAC 1 cut(s) 1039
MseI TTAA 1 cut(s) 1209
MslI CAYNNNNRTG 1 cut(s) 1073
MspA1I CMGCKG 2 cut(s) 319, 809
MspI CCGG 6 cut(s) 560, 590, 620, 713, 821, 918
MspR9I CCNGG 2 cut(s) 417, 919
MunI CAATTG 1 cut(s) 894
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 1 cut(s) 417
MvnI CGCG 2 cut(s) 265, 319
NciI CCSGG 1 cut(s) 919
NdeII GATC 3 cut(s) 10, 324, 940
NlaIII CATG 7 cut(s) 68, 937, 978, 1029, 1095, 1182, 1267
NlaIV GGNNCC 2 cut(s) 339, 846
NmuCI GTSAC 1 cut(s) 1156
PceI AGGCCT 1 cut(s) 1221
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 2 cut(s) 796, 1111
PflFI GACNNNGTC 1 cut(s) 1154
PflMI CCANNNNNTGG 1 cut(s) 334
PfoI TCCNGGA 1 cut(s) 415
PpuMI RGGWCCY 1 cut(s) 419
Psp1406I AACGTT 1 cut(s) 962
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 1 cut(s) 415
PspEI GGTNACC 1 cut(s) 1156
PspGI CCWGG 1 cut(s) 415
PspN4I GGNNCC 2 cut(s) 339, 846
PspPI GGNCC 4 cut(s) 419, 844, 854, 1260
PspPPI RGGWCCY 1 cut(s) 419
PstI CTGCAG 1 cut(s) 640
PsyI GACNNNGTC 1 cut(s) 1154
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 9 cut(s) 75, 291, 395, 624, 755, 972, 1033, 1049, 1199
RsaNI GTAC 9 cut(s) 74, 290, 394, 623, 754, 971, 1032, 1048, 1198
RseI CAYNNNNRTG 1 cut(s) 1073
SacII CCGCGG 1 cut(s) 320
SaqAI TTAA 1 cut(s) 1209
Sau3AI GATC 3 cut(s) 10, 324, 940
Sau96I GGNCC 4 cut(s) 419, 844, 854, 1260
ScrFI CCNGG 2 cut(s) 417, 919
SduI GDGCHC 2 cut(s) 380, 924
SfaNI GCATC 2 cut(s) 706, 828
SfcI CTRYAG 2 cut(s) 636, 1233
Sfr303I CCGCGG 1 cut(s) 320
SgrBI CCGCGG 1 cut(s) 320
SinI GGWCC 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 1073
SmlI CTYRAG 2 cut(s) 660, 825
SmoI CTYRAG 2 cut(s) 660, 825
Sse9I AATT 5 cut(s) 25, 114, 293, 894, 1210
SseBI AGGCCT 1 cut(s) 1221
SspMI CTAG 2 cut(s) 1130, 1223
StuI AGGCCT 1 cut(s) 1221
StyD4I CCNGG 2 cut(s) 415, 917
TaaI ACNGT 4 cut(s) 234, 432, 498, 753
TaiI ACGT 1 cut(s) 965
TaqI TCGA 1 cut(s) 81
TasI AATT 5 cut(s) 25, 114, 293, 894, 1210
TatI WGTACW 2 cut(s) 289, 1197
TfiI GAWTC 2 cut(s) 796, 1111
Tru1I TTAA 1 cut(s) 1209
Tru9I TTAA 1 cut(s) 1209
TscAI CASTG 2 cut(s) 907, 1305
TseFI GTSAC 1 cut(s) 1156
Tsp45I GTSAC 1 cut(s) 1156
TspDTI ATGAA 4 cut(s) 893, 902, 1057, 1072
TspRI CASTG 2 cut(s) 907, 1305
Tth111I GACNNNGTC 1 cut(s) 1154
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 419
XagI CCTNNNNNAGG 4 cut(s) 414, 639, 660, 1233
XspI CTAG 2 cut(s) 1130, 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.