FvH4_6g26810
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000692
Physical Location & Seq
Forward (+)
20504772 .. 20506508
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26810.t1

Sequence Viewer

Length: 1104 bp
ATGGAAAGACTAAACAGCTTTAGACACCTTAACCAAAAATGGTCAAATGGAGAGCATTCCAACGAGCTACTCCATGCTCAAGCCCACATATGGAACCACATCTTCAGCTTCATAAACTCCATGTCCCTCAAATCTGCAATTCAACTAGGTATACCAGATATCATCAACAAACATGGCCGCCCCATGACTCTTTCTGAGCTCACATCTGCCTTACCAATCAACCCAACCAAATCCCACAGCATCTACCGCCTCATGCGAATATTGATTCACTCTGGCTTCTTCGCTAAGAAAAAGCTGAGTAAATCTGATGAGGAAGGTTATGTTCTTACTGATGCATCCCAGCTCCTTCTGAAGGATCACCCCTTGAGCATAACACCTTTCTTAAACGCCATGCTCGACCCTGTTTTGACCAAACCATGGCATTACTTCAGCACTTGGTTCCAAAACGATGACCCTACGCCATTTGACACAGCACATGGGATGACATTTTGGGACTACGGGAATCATCAGCCAAGTATTGCCCATTTCTTCAACGATGCCATGGCTAGCGATGCTCGGTTAGTCACCAGCGTGATTGTCGACGAGTGCAGAGGGGTGTTTGAGGGATTAGATTCATTGGTCGATGTTGGAGGTGGTACAGGAACTGTGGCCAAGGCCATTGCTGATGCGTTCCCACATATTAAGTGCACTGTACTTGATCTCCCACATGTGGTGGCTGACCTGCAAGGAAGTAAGAACTTGAAGTATACTGGAGGTGACATGTTTGAGGCAGTTCCTCCTGCCGATGCAGTTTTACTCAAGTGGATATTGCACGACTGGAATGATGAAGAATGTGTCAAAATACTGGAGCGATCTAAAGAGGCAATTACAGGCAAGGACAAGAAAGGCAAGGTGATTATCATAGATATGATGATGGAGAACCAGAAGGGAGATGAGGAATCAATGGAAACGCAGCTCTTCTTCGACATGCTGATGATGGCCCTTGTCACAGGAAAAGAAAGGAATGAGAAAGAATGGTCTAAGCTCTTCACTGATGCTGGTTTCAGTGACTATAAGATAACTCCCATTCTGGGTTTAAGGTCTCTTATTGAGGTGTATCCTTAA

Protein Analysis

368

Amino Acids

41.44

Weight (kDa)

5.71

Isoelectric Point (pI)

27.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 31 - 119 5.4e-21 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 140 - 349 9.5e-59 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26800 FvH4_6g26810
malus_domestica MD01G1048000.v1.1 MD01G1048300.v1.1 MD01G1048400.v1.1 MD01G1051800.v1.1 MD01G1051900.v1.1 MD01G1149300.v1.1
prunus_persica Prupe.3G118900_v2.0.a1 Prupe.3G119100_v2.0.a1 Prupe.3G119700_v2.0.a1 Prupe.3G119800_v2.0.a1 Prupe.3G120200_v2.0.a1 Prupe.3G120400_v2.0.a1 Prupe.3G127600_v2.0.a1 Prupe.3G129000_v2.0.a1 Prupe.3G129200_v2.0.a1 Prupe.3G129400_v2.0.a1 Prupe.3G129600_v2.0.a1 Prupe.3G129800_v2.0.a1 Prupe.4G252900_v2.0.a1 Prupe.4G253200_v2.0.a1 Prupe.4G253300_v2.0.a1
pyrus_communis pycom01g07360 pycom01g07640 pycom01g07670 pycom01g07720 pycom01g07740 pycom01g07750
rosa_chinensis RchiOBHm_Chr2g0119291 RchiOBHm_Chr2g0123081 RchiOBHm_Chr2g0123091 RchiOBHm_Chr2g0128091 RchiOBHm_Chr2g0128121 RchiOBHm_Chr2g0128161 RchiOBHm_Chr4g0436611
rosa_laevigata RLG00000006507 RLG00000006508 RLG00000018994 RLG00000018995
rosa_multiflora Rmu_co8289337.1_g000001 Rmu_sc0000837.1_g000001 Rmu_sc0003130.1_g000011 Rmu_sc0027975.1_g000001
rosa_roxburghii Rroxscaffold_2G00115570 Rroxscaffold_2G00115600 Rroxscaffold_2G00116080 Rroxscaffold_2G00116090 Rroxscaffold_5G00377660
rosa_rugosa Rorug02G0278000 Rorug02G0278000 Rorug02G0278100 Rorug02G0558700 Rorug04G0294700
rosa_samantha Rh2AG330000 Rh2AG330300 Rh2AG330500 Rh2AG330600 Rh2AG330900 Rh2AG331100 Rh2BG310500 Rh2BG339200 Rh2BG339300 Rh2CG316800 Rh2DG304600 Rh2DG356000 Rh2DG356200 Rh4AG349100 Rh4BG357800 Rh4CG372300 Rh4DG352000 Rh5BG070000
rosa_wichuraiana Rw2G026730 Rw2G026760 Rw4G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 729
AccB7I CCANNNNNTGG 1 cut(s) 417
AccI GTMKAC 3 cut(s) 151, 579, 746
AciI CCGC 2 cut(s) 178, 247
AclWI GGATC 1 cut(s) 363
AcoI YGGCCR 2 cut(s) 175, 648
AcuI CTGAAG 3 cut(s) 88, 371, 412
AfaI GTAC 2 cut(s) 637, 693
AfiI CCNNNNNNNGG 5 cut(s) 90, 352, 417, 709, 1070
AflIII ACRYGT 2 cut(s) 706, 759
AgsI TTSAA 3 cut(s) 143, 532, 742
AleI CACNNNNGTG 1 cut(s) 569
AluBI AGCT 8 cut(s) 18, 67, 108, 199, 295, 343, 955, 1024
AluI AGCT 8 cut(s) 18, 67, 108, 199, 295, 343, 955, 1024
Alw21I GWGCWC 2 cut(s) 201, 689
Alw26I GTCTC 1 cut(s) 1086
Alw44I GTGCAC 1 cut(s) 685
AlwI GGATC 1 cut(s) 363
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 4 cut(s) 175, 648, 654, 978
ApaLI GTGCAC 1 cut(s) 685
ApeKI GCWGC 1 cut(s) 952
AspS9I GGNCC 1 cut(s) 979
AsuHPI GGTGA 4 cut(s) 350, 556, 767, 904
AsuNHI GCTAGC 1 cut(s) 545
BaeGI GKGCMC 1 cut(s) 689
BalI TGGCCA 1 cut(s) 650
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 201, 689
BbvI GCAGC 1 cut(s) 964
BccI CCATC 2 cut(s) 907, 970
BcoDI GTCTC 1 cut(s) 1086
BfaI CTAG 2 cut(s) 146, 546
BfuAI ACCTGC 1 cut(s) 729
BisI GCNGC 2 cut(s) 178, 953
BlsI GCNGC 2 cut(s) 179, 954
BmgT120I GGNCC 1 cut(s) 979
BmiI GGNNCC 2 cut(s) 95, 440
BmsI GCATC 8 cut(s) 249, 322, 344, 526, 541, 655, 775, 1024
BmtI GCTAGC 1 cut(s) 549
BpmI CTGGAG 2 cut(s) 771, 866
BpuEI CTTGAG 3 cut(s) 63, 385, 782
BsaI GGTCTC 1 cut(s) 1086
BsaJI CCNNGG 3 cut(s) 416, 540, 651
BsaXI ACNNNNNCTCC 2 cut(s) 684, 714
Bsc4I CCNNNNNNNGG 5 cut(s) 90, 352, 417, 709, 1070
Bse1I ACTGG 3 cut(s) 754, 821, 849
Bse3DI GCAATG 1 cut(s) 657
BseDI CCNNGG 3 cut(s) 416, 540, 651
BseGI GGATG 2 cut(s) 335, 486
BseLI CCNNNNNNNGG 5 cut(s) 90, 352, 417, 709, 1070
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 2 cut(s) 186, 287
BseNI ACTGG 3 cut(s) 754, 821, 849
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 1 cut(s) 964
BseYI CCCAGC 1 cut(s) 339
BsgI GTGCAG 1 cut(s) 607
BshFI GGCC 4 cut(s) 177, 650, 656, 980
BsiHKAI GWGCWC 2 cut(s) 201, 689
BslFI GGGAC 2 cut(s) 109, 506
BslI CCNNNNNNNGG 5 cut(s) 90, 352, 417, 709, 1070
BsmAI GTCTC 1 cut(s) 1086
BsmFI GGGAC 2 cut(s) 109, 506
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 4 cut(s) 177, 650, 656, 980
Bso31I GGTCTC 1 cut(s) 1086
Bsp1286I GDGCHC 2 cut(s) 201, 689
Bsp143I GATC 3 cut(s) 355, 697, 851
Bsp19I CCATGG 2 cut(s) 416, 540
BspACI CCGC 2 cut(s) 178, 247
BspANI GGCC 4 cut(s) 177, 650, 656, 980
BspCNI CTCAG 2 cut(s) 187, 288
BspLI GGNNCC 2 cut(s) 95, 440
BspMI ACCTGC 1 cut(s) 729
BspOI GCTAGC 1 cut(s) 549
BspPI GGATC 1 cut(s) 363
BspQI GCTCTTC 2 cut(s) 962, 1031
BspTNI GGTCTC 1 cut(s) 1086
BsrDI GCAATG 1 cut(s) 657
BsrI ACTGG 3 cut(s) 754, 821, 849
BssECI CCNNGG 3 cut(s) 416, 540, 651
BssMI GATC 3 cut(s) 355, 697, 851
BssNAI GTATAC 2 cut(s) 152, 747
BssT1I CCWWGG 3 cut(s) 416, 540, 651
Bst1107I GTATAC 2 cut(s) 152, 747
Bst4CI ACNGT 2 cut(s) 646, 691
Bst6I CTCTTC 2 cut(s) 962, 1031
BstC8I GCNNGC 1 cut(s) 547
BstDEI CTNAG 4 cut(s) 195, 285, 296, 1020
BstDSI CCRYGG 2 cut(s) 416, 540
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 335, 486
BstKTI GATC 3 cut(s) 358, 700, 854
BstMAI GTCTC 1 cut(s) 1086
BstMBI GATC 3 cut(s) 355, 697, 851
BstMWI GCNNNNNNNGC 2 cut(s) 246, 551
BstNSI RCATGY 3 cut(s) 710, 763, 970
BstSLI GKGCMC 1 cut(s) 689
BstV1I GCAGC 1 cut(s) 964
BstZ17I GTATAC 2 cut(s) 152, 747
BsuRI GGCC 4 cut(s) 177, 650, 656, 980
BtgI CCRYGG 2 cut(s) 416, 540
BtgZI GCGATG 1 cut(s) 564
BtsCI GGATG 2 cut(s) 335, 486
BtsIMutI CAGTG 3 cut(s) 687, 1029, 1051
BveI ACCTGC 1 cut(s) 729
Cac8I GCNNGC 1 cut(s) 547
CaiI CAGNNNCTG 1 cut(s) 644
Cfr13I GGNCC 1 cut(s) 979
Csp6I GTAC 2 cut(s) 636, 692
CviQI GTAC 2 cut(s) 636, 692
DdeI CTNAG 4 cut(s) 195, 285, 296, 1020
DpnI GATC 3 cut(s) 357, 699, 853
DpnII GATC 3 cut(s) 355, 697, 851
EaeI YGGCCR 2 cut(s) 175, 648
Eam1104I CTCTTC 2 cut(s) 962, 1031
EarI CTCTTC 2 cut(s) 962, 1031
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 3 cut(s) 416, 540, 651
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 1086
Eco32I GATATC 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 3 cut(s) 88, 371, 412
EcoICRI GAGCTC 1 cut(s) 199
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 3 cut(s) 416, 540, 651
EcoT22I ATGCAT 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 3 cut(s) 416, 540, 651
FaqI GGGAC 2 cut(s) 109, 506
FauNDI CATATG 1 cut(s) 89
FblI GTMKAC 3 cut(s) 151, 579, 746
Fnu4HI GCNGC 2 cut(s) 178, 953
FokI GGATG 2 cut(s) 322, 493
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 2 cut(s) 178, 953
FspBI CTAG 2 cut(s) 146, 546
GluI GCNGC 2 cut(s) 178, 953
GsaI CCCAGC 1 cut(s) 343
GsuI CTGGAG 2 cut(s) 771, 866
HaeIII GGCC 4 cut(s) 177, 650, 656, 980
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 5 cut(s) 187, 265, 502, 611, 938
HphI GGTGA 4 cut(s) 350, 556, 767, 904
Hpy166II GTNNAC 4 cut(s) 152, 580, 687, 747
Hpy188I TCNGA 3 cut(s) 196, 307, 351
Hpy8I GTNNAC 4 cut(s) 152, 580, 687, 747
Hpy99I CGWCG 1 cut(s) 584
HpyAV CCTTC 4 cut(s) 308, 346, 356, 919
HpyCH4III ACNGT 2 cut(s) 646, 691
HpyCH4V TGCA 7 cut(s) 137, 335, 588, 687, 724, 788, 811
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 551
HpyF3I CTNAG 4 cut(s) 195, 285, 296, 1020
Kzo9I GATC 3 cut(s) 355, 697, 851
LguI GCTCTTC 2 cut(s) 962, 1031
LmnI GCTCC 2 cut(s) 348, 847
Lsp1109I GCAGC 1 cut(s) 964
LweI GCATC 8 cut(s) 249, 322, 344, 526, 541, 655, 775, 1024
MaeI CTAG 2 cut(s) 146, 546
MaeIII GTNAC 4 cut(s) 562, 755, 985, 1046
MalI GATC 3 cut(s) 357, 699, 853
MboI GATC 3 cut(s) 355, 697, 851
MboII GAAGA 7 cut(s) 94, 271, 520, 839, 949, 952, 1018
MhlI GDGCHC 2 cut(s) 201, 689
MlsI TGGCCA 1 cut(s) 650
MluCI AATT 2 cut(s) 138, 864
MluNI TGGCCA 1 cut(s) 650
MlyI GAGTC 1 cut(s) 181
MmeI TCCRAC 2 cut(s) 84, 607
Mox20I TGGCCA 1 cut(s) 650
Mph1103I ATGCAT 1 cut(s) 337
MscI TGGCCA 1 cut(s) 650
MseI TTAA 5 cut(s) 30, 383, 681, 1076, 1102
MslI CAYNNNNRTG 3 cut(s) 569, 905, 971
Msp20I TGGCCA 1 cut(s) 650
Mva1269I GAATGC 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 246, 551
NcoI CCATGG 2 cut(s) 416, 540
NdeI CATATG 1 cut(s) 89
NdeII GATC 3 cut(s) 355, 697, 851
NheI GCTAGC 1 cut(s) 545
NlaIV GGNNCC 2 cut(s) 95, 440
NmuCI GTSAC 4 cut(s) 562, 755, 985, 1046
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 3 cut(s) 710, 763, 970
OliI CACNNNNGTG 1 cut(s) 569
PciI ACATGT 2 cut(s) 706, 759
PciSI GCTCTTC 2 cut(s) 962, 1031
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 4 cut(s) 265, 502, 611, 938
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 2 cut(s) 179, 954
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PscI ACATGT 2 cut(s) 706, 759
Psp124BI GAGCTC 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 339
PspN4I GGNNCC 2 cut(s) 95, 440
PspPI GGNCC 1 cut(s) 979
PstNI CAGNNNCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 637, 693
RsaNI GTAC 2 cut(s) 636, 692
RseI CAYNNNNRTG 3 cut(s) 569, 905, 971
SacI GAGCTC 1 cut(s) 201
SalI GTCGAC 1 cut(s) 578
SapI GCTCTTC 2 cut(s) 962, 1031
SaqAI TTAA 5 cut(s) 30, 383, 681, 1076, 1102
SatI GCNGC 2 cut(s) 178, 953
Sau3AI GATC 3 cut(s) 355, 697, 851
Sau96I GGNCC 1 cut(s) 979
SchI GAGTC 1 cut(s) 181
SduI GDGCHC 2 cut(s) 201, 689
SfaNI GCATC 8 cut(s) 249, 322, 344, 526, 541, 655, 775, 1024
SmiMI CAYNNNNRTG 3 cut(s) 569, 905, 971
SmlI CTYRAG 3 cut(s) 78, 364, 797
SmoI CTYRAG 3 cut(s) 78, 364, 797
Sse9I AATT 2 cut(s) 138, 864
SsiI CCGC 2 cut(s) 178, 247
SspI AATATT 1 cut(s) 261
SspMI CTAG 2 cut(s) 146, 546
SstI GAGCTC 1 cut(s) 201
StyI CCWWGG 3 cut(s) 416, 540, 651
TaaI ACNGT 2 cut(s) 646, 691
TaqI TCGA 4 cut(s) 396, 579, 621, 963
TasI AATT 2 cut(s) 138, 864
TatI WGTACW 1 cut(s) 691
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 4 cut(s) 265, 502, 611, 938
Tru1I TTAA 5 cut(s) 30, 383, 681, 1076, 1102
Tru9I TTAA 5 cut(s) 30, 383, 681, 1076, 1102
TscAI CASTG 3 cut(s) 694, 1036, 1051
TseFI GTSAC 4 cut(s) 562, 755, 985, 1046
TseI GCWGC 1 cut(s) 952
Tsp45I GTSAC 4 cut(s) 562, 755, 985, 1046
TspDTI ATGAA 3 cut(s) 100, 603, 840
TspRI CASTG 3 cut(s) 694, 1036, 1051
Van91I CCANNNNNTGG 1 cut(s) 417
VneI GTGCAC 1 cut(s) 685
XagI CCTNNNNNAGG 1 cut(s) 350
XceI RCATGY 3 cut(s) 710, 763, 970
XmiI GTMKAC 3 cut(s) 151, 579, 746
XspI CTAG 2 cut(s) 146, 546
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.