Rroxscaffold_2G00115570
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000692
Physical Location & Seq
Reverse (-)
41873698 .. 41875089
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00115570.1

Sequence Viewer

Length: 1107 bp
ATGGAAAGACTGAATAGCTTTAAGCACCTTAACCATAAGTGGGCTAGTGGAGAGCATTCTACCCAGCTTCTTCAAGCTCAAGCCCACATATGGAAACACTTTTTCAGCTTCATAAACTGCATGACCCTTAAATCTGCAATTGAACTAGGTATACCAGATATCATCAAAAAACATGGCCGCCCCATAACTCTTTCTGAGCTCACATCTGCATTACCAATTCACCCAACAAAAGTCCTCAGTGTTTACCGCCTCATGCGTATATTGGTCCGCTCCGGCTTCTTTGCAAAAAAAGAGCTGAATGAACTGGATGAGGAAGAAGGTTATATACTTACTGACGCTTCTCAGCTGCTCCTTAAGGATCACCCCTTCAGTATAACACCATTTCTAATGGCTGTGCTCAACCCTAACTTCACAAACACATGCCATTATATGAGCACTTGGTTCCAAAATGATGACCCTACTCCATTTAACACGGCATATGGGATGACGTTTTGGGACTACATCACCCAGGAGCCAAGTCATGCCAACCTTTTCAATGATGCCATGGCTAGTGATACTCGTTTGGTGACCAGCGTGTTACTTAAAGAGTGCAAGGGGGTATTCGAGGGATTGAAATCATTAGTTGATGTTGGGGGTGGTACAGGAACAATGGCCAAGGCCATTGTTGATGCATTCCCAGAAATTAACTGCACCGTACTTGATCTCCCACATGTGGTGGCTGATCTGCAAGGAAGTAAGAACCTGAAATATGCTGGAGGGGACATGTTTGAGGCAGTTCCTCCGGCAGATGCAATTATGTTGAAGTGTATATTGCATGACTGGAGTGATGAAGAATCTGTGAAAATACTTAAGCGATGTAAAGAGGCAATAACAAGTAATAAGAAGAAAGGCAAGGTGATTATCATAGATATGATTATGGAAAACCAGAAAGGAGATGAGGAATCAATTGAGACACAACTGTTCTTCGACATGACGATGATGATGGTGGTCACGGGAAAAGAAAGGAATGAAAAAGAATGGGCTAAGCTCTTTTCTGATGCAGGTTTCAGTGACTATAAGATAACTCCCAGTGTGGGTGTAAGATCTCTCATTGAGGTTTATCCTTGA

Protein Analysis

368

Amino Acids

41.53

Weight (kDa)

5.76

Isoelectric Point (pI)

32.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 31 - 120 4.7e-20 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 143 - 350 2.1e-59 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26800 FvH4_6g26810
malus_domestica MD01G1048000.v1.1 MD01G1048300.v1.1 MD01G1048400.v1.1 MD01G1051800.v1.1 MD01G1051900.v1.1 MD01G1149300.v1.1
prunus_persica Prupe.3G118900_v2.0.a1 Prupe.3G119100_v2.0.a1 Prupe.3G119700_v2.0.a1 Prupe.3G119800_v2.0.a1 Prupe.3G120200_v2.0.a1 Prupe.3G120400_v2.0.a1 Prupe.3G127600_v2.0.a1 Prupe.3G129000_v2.0.a1 Prupe.3G129200_v2.0.a1 Prupe.3G129400_v2.0.a1 Prupe.3G129600_v2.0.a1 Prupe.3G129800_v2.0.a1 Prupe.4G252900_v2.0.a1 Prupe.4G253200_v2.0.a1 Prupe.4G253300_v2.0.a1
pyrus_communis pycom01g07360 pycom01g07640 pycom01g07670 pycom01g07720 pycom01g07740 pycom01g07750
rosa_chinensis RchiOBHm_Chr2g0119291 RchiOBHm_Chr2g0123081 RchiOBHm_Chr2g0123091 RchiOBHm_Chr2g0128091 RchiOBHm_Chr2g0128121 RchiOBHm_Chr2g0128161 RchiOBHm_Chr4g0436611
rosa_laevigata RLG00000006507 RLG00000006508 RLG00000018994 RLG00000018995
rosa_multiflora Rmu_co8289337.1_g000001 Rmu_sc0000837.1_g000001 Rmu_sc0003130.1_g000011 Rmu_sc0027975.1_g000001
rosa_roxburghii Rroxscaffold_2G00115570 Rroxscaffold_2G00115600 Rroxscaffold_2G00116080 Rroxscaffold_2G00116090 Rroxscaffold_5G00377660
rosa_rugosa Rorug02G0278000 Rorug02G0278000 Rorug02G0278100 Rorug02G0558700 Rorug04G0294700
rosa_samantha Rh2AG330000 Rh2AG330300 Rh2AG330500 Rh2AG330600 Rh2AG330900 Rh2AG331100 Rh2BG310500 Rh2BG339200 Rh2BG339300 Rh2CG316800 Rh2DG304600 Rh2DG356000 Rh2DG356200 Rh4AG349100 Rh4BG357800 Rh4CG372300 Rh4DG352000 Rh5BG070000
rosa_wichuraiana Rw2G026730 Rw2G026760 Rw4G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1031
AccBSI CCGCTC 1 cut(s) 270
AccI GTMKAC 1 cut(s) 151
AciI CCGC 3 cut(s) 178, 247, 268
AclWI GGATC 1 cut(s) 366
AcoI YGGCCR 2 cut(s) 175, 651
AcuI CTGAAG 1 cut(s) 352
AfaI GTAC 2 cut(s) 640, 696
AfiI CCNNNNNNNGG 4 cut(s) 40, 90, 712, 1073
AflII CTTAAG 2 cut(s) 353, 848
AflIII ACRYGT 2 cut(s) 709, 762
AgsI TTSAA 5 cut(s) 74, 143, 535, 613, 802
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 8 cut(s) 18, 67, 77, 108, 199, 295, 346, 1027
AluI AGCT 8 cut(s) 18, 67, 77, 108, 199, 295, 346, 1027
Alw21I GWGCWC 3 cut(s) 201, 399, 437
Alw26I GTCTC 1 cut(s) 944
AlwI GGATC 1 cut(s) 366
AoxI GGCC 3 cut(s) 175, 651, 657
ApeKI GCWGC 1 cut(s) 346
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 5 cut(s) 212, 353, 496, 577, 907
AvaII GGWCC 1 cut(s) 265
BalI TGGCCA 1 cut(s) 653
BanII GRGCYC 1 cut(s) 201
BarI GAAGNNNNNNTAC 2 cut(s) 309, 341
Bbv12I GWGCWC 3 cut(s) 201, 399, 437
BbvI GCAGC 1 cut(s) 333
BccI CCATC 1 cut(s) 976
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 1 cut(s) 509
BcoDI GTCTC 1 cut(s) 944
BfaI CTAG 3 cut(s) 45, 146, 549
BfrI CTTAAG 2 cut(s) 353, 848
BfuAI ACCTGC 1 cut(s) 1031
BglII AGATCT 1 cut(s) 1082
BisI GCNGC 2 cut(s) 178, 347
BlpI GCTNAGC 1 cut(s) 1023
BlsI GCNGC 2 cut(s) 179, 348
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 2 cut(s) 443, 513
BmrFI CCNGG 1 cut(s) 509
BmrI ACTGGG 1 cut(s) 1062
BmsI GCATC 4 cut(s) 529, 658, 778, 1027
BmuI ACTGGG 1 cut(s) 1062
BpmI CTGGAG 2 cut(s) 774, 841
Bpu1102I GCTNAGC 1 cut(s) 1023
BpuEI CTTGAG 1 cut(s) 63
BsaBI GATNNNNATC 1 cut(s) 613
BsaJI CCNNGG 3 cut(s) 507, 543, 654
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 90, 712, 1073
Bse1I ACTGG 3 cut(s) 309, 824, 1068
Bse8I GATNNNNATC 1 cut(s) 613
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 3 cut(s) 507, 543, 654
BseGI GGATG 2 cut(s) 313, 489
BseJI GATNNNNATC 1 cut(s) 613
BseLI CCNNNNNNNGG 4 cut(s) 40, 90, 712, 1073
BseMII CTCAG 3 cut(s) 186, 250, 356
BseNI ACTGG 3 cut(s) 309, 824, 1068
BseXI GCAGC 1 cut(s) 333
BseYI CCCAGC 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 673
BshFI GGCC 3 cut(s) 177, 653, 659
BsiHKAI GWGCWC 3 cut(s) 201, 399, 437
BsiSI CCGG 2 cut(s) 273, 782
BslFI GGGAC 2 cut(s) 509, 773
BslI CCNNNNNNNGG 4 cut(s) 40, 90, 712, 1073
BsmAI GTCTC 1 cut(s) 944
BsmFI GGGAC 2 cut(s) 509, 773
BsmI GAATGC 2 cut(s) 55, 671
BsnI GGCC 3 cut(s) 177, 653, 659
Bsp1286I GDGCHC 3 cut(s) 201, 399, 437
Bsp143I GATC 4 cut(s) 358, 700, 721, 1082
Bsp1720I GCTNAGC 1 cut(s) 1023
Bsp19I CCATGG 1 cut(s) 543
BspACI CCGC 3 cut(s) 178, 247, 268
BspANI GGCC 3 cut(s) 177, 653, 659
BspCNI CTCAG 3 cut(s) 187, 249, 355
BspLI GGNNCC 2 cut(s) 443, 513
BspMI ACCTGC 1 cut(s) 1031
BspPI GGATC 1 cut(s) 366
BspTI CTTAAG 2 cut(s) 353, 848
BsrBI CCGCTC 1 cut(s) 270
BsrI ACTGG 3 cut(s) 309, 824, 1068
BssECI CCNNGG 3 cut(s) 507, 543, 654
BssMI GATC 4 cut(s) 358, 700, 721, 1082
BssNAI GTATAC 1 cut(s) 152
BssT1I CCWWGG 2 cut(s) 543, 654
Bst1107I GTATAC 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 2 cut(s) 694, 960
BstAFI CTTAAG 2 cut(s) 353, 848
BstDEI CTNAG 4 cut(s) 195, 236, 342, 1023
BstDSI CCRYGG 1 cut(s) 543
BstEII GGTNACC 1 cut(s) 565
BstF5I GGATG 2 cut(s) 313, 489
BstKTI GATC 4 cut(s) 361, 703, 724, 1085
BstMAI GTCTC 1 cut(s) 944
BstMBI GATC 4 cut(s) 358, 700, 721, 1082
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 3 cut(s) 423, 713, 766
BstPI GGTNACC 1 cut(s) 565
BstSCI CCNGG 1 cut(s) 507
BstV1I GCAGC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 1082
BstYI RGATCY 1 cut(s) 1082
BstZ17I GTATAC 1 cut(s) 152
BsuRI GGCC 3 cut(s) 177, 653, 659
BtgI CCRYGG 1 cut(s) 543
BtgZI GCGATG 1 cut(s) 868
BtsCI GGATG 2 cut(s) 313, 489
BtsIMutI CAGTG 3 cut(s) 244, 1054, 1075
BveI ACCTGC 1 cut(s) 1031
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 1 cut(s) 344
Csp6I GTAC 2 cut(s) 639, 695
CviQI GTAC 2 cut(s) 639, 695
DdeI CTNAG 4 cut(s) 195, 236, 342, 1023
DpnI GATC 4 cut(s) 360, 702, 723, 1084
DpnII GATC 4 cut(s) 358, 700, 721, 1082
EaeI YGGCCR 2 cut(s) 175, 651
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 543, 654
Eco24I GRGCYC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 352
Eco91I GGTNACC 1 cut(s) 565
EcoICRI GAGCTC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 507
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 2 cut(s) 543, 654
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 2 cut(s) 543, 654
FaqI GGGAC 2 cut(s) 509, 773
FauNDI CATATG 2 cut(s) 89, 478
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 2 cut(s) 178, 347
FokI GGATG 2 cut(s) 320, 496
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 2 cut(s) 178, 347
FspBI CTAG 3 cut(s) 45, 146, 549
GluI GCNGC 2 cut(s) 178, 347
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 2 cut(s) 774, 841
HaeIII GGCC 3 cut(s) 177, 653, 659
HapII CCGG 2 cut(s) 273, 782
HgaI GACGC 1 cut(s) 344
HinfI GANTC 2 cut(s) 833, 941
HpaII CCGG 2 cut(s) 273, 782
HphI GGTGA 5 cut(s) 212, 353, 496, 577, 907
Hpy166II GTNNAC 2 cut(s) 152, 244
Hpy188I TCNGA 2 cut(s) 196, 1036
Hpy8I GTNNAC 2 cut(s) 152, 244
HpyAV CCTTC 2 cut(s) 311, 376
HpyCH4III ACNGT 2 cut(s) 694, 960
HpyCH4IV ACGT 1 cut(s) 488
HpyF3I CTNAG 4 cut(s) 195, 236, 342, 1023
HpySE526I ACGT 1 cut(s) 488
Kzo9I GATC 4 cut(s) 358, 700, 721, 1082
LmnI GCTCC 3 cut(s) 275, 354, 511
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 4 cut(s) 529, 658, 778, 1027
MaeI CTAG 3 cut(s) 45, 146, 549
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 4 cut(s) 565, 576, 988, 1049
MalI GATC 4 cut(s) 360, 702, 723, 1084
MbiI CCGCTC 1 cut(s) 270
MboI GATC 4 cut(s) 358, 700, 721, 1082
MboII GAAGA 5 cut(s) 62, 326, 842, 895, 955
MfeI CAATTG 2 cut(s) 138, 945
MflI RGATCY 1 cut(s) 1082
MhlI GDGCHC 3 cut(s) 201, 399, 437
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 5 cut(s) 138, 216, 681, 792, 945
MluNI TGGCCA 1 cut(s) 653
Mox20I TGGCCA 1 cut(s) 653
Mph1103I ATGCAT 1 cut(s) 673
MscI TGGCCA 1 cut(s) 653
MseI TTAA 8 cut(s) 21, 30, 129, 354, 468, 582, 684, 849
MslI CAYNNNNRTG 2 cut(s) 908, 974
Msp20I TGGCCA 1 cut(s) 653
MspA1I CMGCKG 1 cut(s) 346
MspCI CTTAAG 2 cut(s) 353, 848
MspI CCGG 2 cut(s) 273, 782
MspR9I CCNGG 1 cut(s) 509
MunI CAATTG 2 cut(s) 138, 945
Mva1269I GAATGC 2 cut(s) 55, 671
MvaI CCWGG 1 cut(s) 509
NcoI CCATGG 1 cut(s) 543
NdeI CATATG 2 cut(s) 89, 478
NdeII GATC 4 cut(s) 358, 700, 721, 1082
NlaIV GGNNCC 2 cut(s) 443, 513
NmuCI GTSAC 3 cut(s) 565, 988, 1049
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 3 cut(s) 423, 713, 766
PciI ACATGT 2 cut(s) 709, 762
PctI GAATGC 2 cut(s) 55, 671
PfeI GAWTC 2 cut(s) 833, 941
PkrI GCNGC 2 cut(s) 179, 348
PscI ACATGT 2 cut(s) 709, 762
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 507
PspEI GGTNACC 1 cut(s) 565
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 2 cut(s) 443, 513
PspPI GGNCC 1 cut(s) 265
PsrI GAACNNNNNNTAC 2 cut(s) 135, 167
PsuI RGATCY 1 cut(s) 1082
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 2 cut(s) 640, 696
RsaNI GTAC 2 cut(s) 639, 695
RseI CAYNNNNRTG 2 cut(s) 908, 974
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 8 cut(s) 21, 30, 129, 354, 468, 582, 684, 849
SatI GCNGC 2 cut(s) 178, 347
Sau3AI GATC 4 cut(s) 358, 700, 721, 1082
Sau96I GGNCC 1 cut(s) 265
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 3 cut(s) 201, 399, 437
SfaNI GCATC 4 cut(s) 529, 658, 778, 1027
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 908, 974
SmlI CTYRAG 3 cut(s) 78, 353, 848
SmoI CTYRAG 3 cut(s) 78, 353, 848
Sse9I AATT 5 cut(s) 138, 216, 681, 792, 945
SsiI CCGC 3 cut(s) 178, 247, 268
SspMI CTAG 3 cut(s) 45, 146, 549
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 2 cut(s) 543, 654
TaaI ACNGT 2 cut(s) 694, 960
TaiI ACGT 1 cut(s) 491
TaqI TCGA 2 cut(s) 603, 966
TasI AATT 5 cut(s) 138, 216, 681, 792, 945
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 2 cut(s) 833, 941
Tru1I TTAA 8 cut(s) 21, 30, 129, 354, 468, 582, 684, 849
Tru9I TTAA 8 cut(s) 21, 30, 129, 354, 468, 582, 684, 849
TscAI CASTG 3 cut(s) 244, 1054, 1075
TseFI GTSAC 3 cut(s) 565, 988, 1049
TseI GCWGC 1 cut(s) 346
Tsp45I GTSAC 3 cut(s) 565, 988, 1049
TspDTI ATGAA 4 cut(s) 100, 315, 843, 1023
TspRI CASTG 3 cut(s) 244, 1054, 1075
Vha464I CTTAAG 2 cut(s) 353, 848
VpaK11BI GGWCC 1 cut(s) 265
XceI RCATGY 3 cut(s) 423, 713, 766
XmiI GTMKAC 1 cut(s) 151
XspI CTAG 3 cut(s) 45, 146, 549
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.