Rroxscaffold_2G00116090
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000692
Physical Location & Seq
Reverse (-)
42918889 .. 42920318
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116090.1

Sequence Viewer

Length: 1104 bp
ATGGAAAGGTTAAACAGCTTTAGACACCTTAACCAAAAATGGTCAAATGGAGAGCATTCCAACGAGCTACTTCATGCTCAAGCCCACATCTGGAACCACATCTTCAGCTTCATAAACTCCATGTCCCTCAAATCTGCAATTCAACTAGGTATACCAGATATCATCAACAAACATGGTTACCCCATGACTCTTTCTGAGCTCACATCTGCACTACCAATCCACCCAACCAAATCCCACAGCGTCTACCGCCTCATGCGAATATTGGTACACTCTGGCTTCTTTGCTAAGAAAAAGCTGAGTAAAACTGATGAGGAAGGTTATACCCTTACTGATGCTTCCCAGCTCCTTCTGAAGGATCATCCCTTAAGCCTAACGCCCTTCTTAACCGCCATGCTCGACCCTGTTTTGACCAAACCATGGAATTACTTGAGCACTTGGTTCCAAAACGATGACCCTACGCCATTTGACACAGCACATGGGATGACATTTTGGGATTACGGGAACCATCAGCCAAGTATTGCTCATTTCTTCAACGATGCCATGGCTAGCGATGCTCGGTTGGTCACCAGCGTGATCATCGATGATTGCAAAGGAGTGTTTGAGGGATTAGAGTCATTGGTCGATGTTGGAGGTGGTACAGGAACTGTGGCAAAGGCCATTGCTGATGCATTCCCACATATTGAATGCACTGTACTTGATCTCCCACATGTGGTGGCTGACCTGCAAGGAAGTAAGAACTTGAAATATACTGGAGGTGACATGTTTGAGGCAGTTCCTCCTGCCGATACAGTTTTACTCAAGTGGATATTGCATGATTGGAACGATGAAGAATGTGTCAAAATACTTGAGCGATCTAGAGAGGCAATTACAAGCAAGGACAAGAAAGGCAAGGTGATTATCATAGATATGATGATGGAGAACCAGAAAGGCGATGAGGAATCAATTGAAACGCAGCTGTTCTTCGACATGCTGATGATGGCCCTCGTCACAGGAAAAGAAAGGAATGAGAAAGAATGGGCTAAGCTCTTCACTGATGCTGGTTTCAGTGACTATAAGATAACTCCTATTTTGGGTTTAAGGTCTCTCATTGAGGTTTATCCTTGA

Protein Analysis

367

Amino Acids

41.43

Weight (kDa)

5.48

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 31 - 119 1.5e-21 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 140 - 349 6.6e-59 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26800 FvH4_6g26810
malus_domestica MD01G1048000.v1.1 MD01G1048300.v1.1 MD01G1048400.v1.1 MD01G1051800.v1.1 MD01G1051900.v1.1 MD01G1149300.v1.1
prunus_persica Prupe.3G118900_v2.0.a1 Prupe.3G119100_v2.0.a1 Prupe.3G119700_v2.0.a1 Prupe.3G119800_v2.0.a1 Prupe.3G120200_v2.0.a1 Prupe.3G120400_v2.0.a1 Prupe.3G127600_v2.0.a1 Prupe.3G129000_v2.0.a1 Prupe.3G129200_v2.0.a1 Prupe.3G129400_v2.0.a1 Prupe.3G129600_v2.0.a1 Prupe.3G129800_v2.0.a1 Prupe.4G252900_v2.0.a1 Prupe.4G253200_v2.0.a1 Prupe.4G253300_v2.0.a1
pyrus_communis pycom01g07360 pycom01g07640 pycom01g07670 pycom01g07720 pycom01g07740 pycom01g07750
rosa_chinensis RchiOBHm_Chr2g0119291 RchiOBHm_Chr2g0123081 RchiOBHm_Chr2g0123091 RchiOBHm_Chr2g0128091 RchiOBHm_Chr2g0128121 RchiOBHm_Chr2g0128161 RchiOBHm_Chr4g0436611
rosa_laevigata RLG00000006507 RLG00000006508 RLG00000018994 RLG00000018995
rosa_multiflora Rmu_co8289337.1_g000001 Rmu_sc0000837.1_g000001 Rmu_sc0003130.1_g000011 Rmu_sc0027975.1_g000001
rosa_roxburghii Rroxscaffold_2G00115570 Rroxscaffold_2G00115600 Rroxscaffold_2G00116080 Rroxscaffold_2G00116090 Rroxscaffold_5G00377660
rosa_rugosa Rorug02G0278000 Rorug02G0278000 Rorug02G0278100 Rorug02G0558700 Rorug04G0294700
rosa_samantha Rh2AG330000 Rh2AG330300 Rh2AG330500 Rh2AG330600 Rh2AG330900 Rh2AG331100 Rh2BG310500 Rh2BG339200 Rh2BG339300 Rh2CG316800 Rh2DG304600 Rh2DG356000 Rh2DG356200 Rh4AG349100 Rh4BG357800 Rh4CG372300 Rh4DG352000 Rh5BG070000
rosa_wichuraiana Rw2G026730 Rw2G026760 Rw4G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 729
AccB7I CCANNNNNTGG 1 cut(s) 417
AccI GTMKAC 2 cut(s) 151, 243
AciI CCGC 2 cut(s) 247, 387
AclWI GGATC 1 cut(s) 363
AcuI CTGAAG 2 cut(s) 88, 371
AfaI GTAC 3 cut(s) 267, 637, 693
AfiI CCNNNNNNNGG 5 cut(s) 90, 352, 417, 709, 1070
AflII CTTAAG 1 cut(s) 364
AflIII ACRYGT 2 cut(s) 706, 759
AgsI TTSAA 5 cut(s) 143, 532, 683, 742, 947
AleI CACNNNNGTG 1 cut(s) 569
AluBI AGCT 8 cut(s) 18, 67, 108, 199, 295, 343, 955, 1024
AluI AGCT 8 cut(s) 18, 67, 108, 199, 295, 343, 955, 1024
Alw21I GWGCWC 2 cut(s) 201, 434
Alw26I GTCTC 1 cut(s) 1086
AlwI GGATC 1 cut(s) 363
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 2 cut(s) 654, 978
ApeKI GCWGC 1 cut(s) 952
AspS9I GGNCC 1 cut(s) 979
AsuHPI GGTGA 3 cut(s) 556, 767, 904
AsuNHI GCTAGC 1 cut(s) 545
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 201, 434
BbvI GCAGC 1 cut(s) 964
BccI CCATC 3 cut(s) 513, 907, 970
BclI TGATCA 1 cut(s) 573
BcoDI GTCTC 1 cut(s) 1086
BfaI CTAG 3 cut(s) 146, 546, 855
BfrI CTTAAG 1 cut(s) 364
BfuAI ACCTGC 1 cut(s) 729
BisI GCNGC 1 cut(s) 953
BlpI GCTNAGC 1 cut(s) 1020
BlsI GCNGC 1 cut(s) 954
BmgT120I GGNCC 1 cut(s) 979
BmiI GGNNCC 3 cut(s) 95, 440, 503
BmsI GCATC 5 cut(s) 322, 526, 541, 655, 1024
BmtI GCTAGC 1 cut(s) 549
BpmI CTGGAG 1 cut(s) 771
Bpu1102I GCTNAGC 1 cut(s) 1020
BpuEI CTTGAG 4 cut(s) 63, 448, 782, 866
Bsa29I ATCGAT 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 1086
BsaJI CCNNGG 2 cut(s) 416, 540
BsaXI ACNNNNNCTCC 2 cut(s) 684, 714
Bsc4I CCNNNNNNNGG 5 cut(s) 90, 352, 417, 709, 1070
Bse1I ACTGG 1 cut(s) 754
Bse3DI GCAATG 1 cut(s) 657
BseCI ATCGAT 1 cut(s) 579
BseDI CCNNGG 2 cut(s) 416, 540
BseGI GGATG 2 cut(s) 358, 486
BseLI CCNNNNNNNGG 5 cut(s) 90, 352, 417, 709, 1070
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 2 cut(s) 186, 287
BseNI ACTGG 1 cut(s) 754
BseXI GCAGC 1 cut(s) 964
BseYI CCCAGC 1 cut(s) 339
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 2 cut(s) 656, 980
BshVI ATCGAT 1 cut(s) 579
BsiHKAI GWGCWC 2 cut(s) 201, 434
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 5 cut(s) 90, 352, 417, 709, 1070
BsmAI GTCTC 1 cut(s) 1086
BsmFI GGGAC 1 cut(s) 109
BsmI GAATGC 3 cut(s) 55, 668, 689
BsnI GGCC 2 cut(s) 656, 980
Bso31I GGTCTC 1 cut(s) 1086
Bsp1286I GDGCHC 2 cut(s) 201, 434
Bsp143I GATC 4 cut(s) 355, 573, 697, 851
Bsp1720I GCTNAGC 1 cut(s) 1020
Bsp19I CCATGG 2 cut(s) 416, 540
BspACI CCGC 2 cut(s) 247, 387
BspANI GGCC 2 cut(s) 656, 980
BspCNI CTCAG 2 cut(s) 187, 288
BspDI ATCGAT 1 cut(s) 579
BspLI GGNNCC 3 cut(s) 95, 440, 503
BspMI ACCTGC 1 cut(s) 729
BspOI GCTAGC 1 cut(s) 549
BspPI GGATC 1 cut(s) 363
BspQI GCTCTTC 1 cut(s) 1031
BspTI CTTAAG 1 cut(s) 364
BspTNI GGTCTC 1 cut(s) 1086
BsrDI GCAATG 1 cut(s) 657
BsrI ACTGG 1 cut(s) 754
BssECI CCNNGG 2 cut(s) 416, 540
BssMI GATC 4 cut(s) 355, 573, 697, 851
BssNAI GTATAC 1 cut(s) 152
BssT1I CCWWGG 2 cut(s) 416, 540
Bst1107I GTATAC 1 cut(s) 152
Bst4CI ACNGT 3 cut(s) 646, 691, 790
Bst6I CTCTTC 1 cut(s) 1031
BstAFI CTTAAG 1 cut(s) 364
BstC8I GCNNGC 1 cut(s) 547
BstDEI CTNAG 4 cut(s) 195, 285, 296, 1020
BstDSI CCRYGG 2 cut(s) 416, 540
BstEII GGTNACC 2 cut(s) 176, 562
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 358, 486
BstKTI GATC 4 cut(s) 358, 576, 700, 854
BstMAI GTCTC 1 cut(s) 1086
BstMBI GATC 4 cut(s) 355, 573, 697, 851
BstMWI GCNNNNNNNGC 2 cut(s) 246, 551
BstNSI RCATGY 3 cut(s) 710, 763, 970
BstPI GGTNACC 2 cut(s) 176, 562
BstV1I GCAGC 1 cut(s) 964
BstZ17I GTATAC 1 cut(s) 152
Bsu15I ATCGAT 1 cut(s) 579
BsuRI GGCC 2 cut(s) 656, 980
BsuTUI ATCGAT 1 cut(s) 579
BtgI CCRYGG 2 cut(s) 416, 540
BtgZI GCGATG 2 cut(s) 564, 945
BtsCI GGATG 2 cut(s) 358, 486
BtsIMutI CAGTG 3 cut(s) 687, 1029, 1051
BveI ACCTGC 1 cut(s) 729
Cac8I GCNNGC 1 cut(s) 547
CaiI CAGNNNCTG 1 cut(s) 644
Cfr13I GGNCC 1 cut(s) 979
ClaI ATCGAT 1 cut(s) 579
CseI GACGC 1 cut(s) 229
Csp6I GTAC 3 cut(s) 266, 636, 692
CviQI GTAC 3 cut(s) 266, 636, 692
DdeI CTNAG 4 cut(s) 195, 285, 296, 1020
DpnI GATC 4 cut(s) 357, 575, 699, 853
DpnII GATC 4 cut(s) 355, 573, 697, 851
Eam1104I CTCTTC 1 cut(s) 1031
EarI CTCTTC 1 cut(s) 1031
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 416, 540
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 1086
Eco32I GATATC 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 2 cut(s) 88, 371
Eco91I GGTNACC 2 cut(s) 176, 562
EcoICRI GAGCTC 1 cut(s) 199
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoO65I GGTNACC 2 cut(s) 176, 562
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 2 cut(s) 416, 540
EcoT22I ATGCAT 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 2 cut(s) 416, 540
FaqI GGGAC 1 cut(s) 109
FbaI TGATCA 1 cut(s) 573
FblI GTMKAC 2 cut(s) 151, 243
Fnu4HI GCNGC 1 cut(s) 953
FokI GGATG 2 cut(s) 345, 493
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 1 cut(s) 953
FspBI CTAG 3 cut(s) 146, 546, 855
GluI GCNGC 1 cut(s) 953
GsaI CCCAGC 1 cut(s) 343
GsuI CTGGAG 1 cut(s) 771
HaeIII GGCC 2 cut(s) 656, 980
HgaI GACGC 1 cut(s) 229
HinfI GANTC 3 cut(s) 187, 611, 938
HphI GGTGA 3 cut(s) 556, 767, 904
Hpy166II GTNNAC 3 cut(s) 152, 244, 268
Hpy188I TCNGA 2 cut(s) 196, 351
Hpy188III TCNNGA 2 cut(s) 91, 855
Hpy8I GTNNAC 3 cut(s) 152, 244, 268
HpyAV CCTTC 4 cut(s) 308, 346, 356, 388
HpyCH4III ACNGT 3 cut(s) 646, 691, 790
HpyCH4V TGCA 7 cut(s) 137, 209, 588, 668, 687, 724, 811
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 551
HpyF3I CTNAG 4 cut(s) 195, 285, 296, 1020
Ksp22I TGATCA 1 cut(s) 573
Kzo9I GATC 4 cut(s) 355, 573, 697, 851
LguI GCTCTTC 1 cut(s) 1031
LmnI GCTCC 1 cut(s) 348
Lsp1109I GCAGC 1 cut(s) 964
LweI GCATC 5 cut(s) 322, 526, 541, 655, 1024
MaeI CTAG 3 cut(s) 146, 546, 855
MaeIII GTNAC 5 cut(s) 176, 562, 755, 985, 1046
MalI GATC 4 cut(s) 357, 575, 699, 853
MboI GATC 4 cut(s) 355, 573, 697, 851
MboII GAAGA 5 cut(s) 94, 520, 839, 952, 1018
MfeI CAATTG 1 cut(s) 942
MhlI GDGCHC 2 cut(s) 201, 434
MluCI AATT 4 cut(s) 138, 421, 864, 942
MlyI GAGTC 2 cut(s) 181, 620
MmeI TCCRAC 2 cut(s) 84, 607
Mph1103I ATGCAT 1 cut(s) 670
MseI TTAA 5 cut(s) 11, 30, 365, 383, 1076
MslI CAYNNNNRTG 3 cut(s) 569, 905, 971
MspA1I CMGCKG 1 cut(s) 955
MspCI CTTAAG 1 cut(s) 364
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 3 cut(s) 55, 668, 689
MwoI GCNNNNNNNGC 2 cut(s) 246, 551
NcoI CCATGG 2 cut(s) 416, 540
NdeII GATC 4 cut(s) 355, 573, 697, 851
NheI GCTAGC 1 cut(s) 545
NlaIV GGNNCC 3 cut(s) 95, 440, 503
NmuCI GTSAC 4 cut(s) 562, 755, 985, 1046
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 3 cut(s) 710, 763, 970
OliI CACNNNNGTG 1 cut(s) 569
PciI ACATGT 2 cut(s) 706, 759
PciSI GCTCTTC 1 cut(s) 1031
PctI GAATGC 3 cut(s) 55, 668, 689
PfeI GAWTC 1 cut(s) 938
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 1 cut(s) 954
PleI GAGTC 2 cut(s) 181, 619
PpsI GAGTC 2 cut(s) 181, 619
PscI ACATGT 2 cut(s) 706, 759
Psp124BI GAGCTC 1 cut(s) 201
PspEI GGTNACC 2 cut(s) 176, 562
PspFI CCCAGC 1 cut(s) 339
PspN4I GGNNCC 3 cut(s) 95, 440, 503
PspPI GGNCC 1 cut(s) 979
PstNI CAGNNNCTG 1 cut(s) 644
PvuII CAGCTG 1 cut(s) 955
RsaI GTAC 3 cut(s) 267, 637, 693
RsaNI GTAC 3 cut(s) 266, 636, 692
RseI CAYNNNNRTG 3 cut(s) 569, 905, 971
SacI GAGCTC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 1031
SaqAI TTAA 5 cut(s) 11, 30, 365, 383, 1076
SatI GCNGC 1 cut(s) 953
Sau3AI GATC 4 cut(s) 355, 573, 697, 851
Sau96I GGNCC 1 cut(s) 979
SchI GAGTC 2 cut(s) 181, 620
SduI GDGCHC 2 cut(s) 201, 434
SfaNI GCATC 5 cut(s) 322, 526, 541, 655, 1024
SmiMI CAYNNNNRTG 3 cut(s) 569, 905, 971
SmlI CTYRAG 5 cut(s) 78, 364, 427, 797, 845
SmoI CTYRAG 5 cut(s) 78, 364, 427, 797, 845
Sse9I AATT 4 cut(s) 138, 421, 864, 942
SsiI CCGC 2 cut(s) 247, 387
SspI AATATT 1 cut(s) 261
SspMI CTAG 3 cut(s) 146, 546, 855
SstI GAGCTC 1 cut(s) 201
StyI CCWWGG 2 cut(s) 416, 540
TaaI ACNGT 3 cut(s) 646, 691, 790
TaqI TCGA 4 cut(s) 396, 579, 621, 963
TasI AATT 4 cut(s) 138, 421, 864, 942
TatI WGTACW 1 cut(s) 691
TfiI GAWTC 1 cut(s) 938
Tru1I TTAA 5 cut(s) 11, 30, 365, 383, 1076
Tru9I TTAA 5 cut(s) 11, 30, 365, 383, 1076
TscAI CASTG 3 cut(s) 694, 1036, 1051
TseFI GTSAC 4 cut(s) 562, 755, 985, 1046
TseI GCWGC 1 cut(s) 952
Tsp45I GTSAC 4 cut(s) 562, 755, 985, 1046
TspDTI ATGAA 3 cut(s) 62, 100, 840
TspRI CASTG 3 cut(s) 694, 1036, 1051
Van91I CCANNNNNTGG 1 cut(s) 417
Vha464I CTTAAG 1 cut(s) 364
XagI CCTNNNNNAGG 1 cut(s) 350
XbaI TCTAGA 1 cut(s) 854
XceI RCATGY 3 cut(s) 710, 763, 970
XmiI GTMKAC 2 cut(s) 151, 243
XspI CTAG 3 cut(s) 146, 546, 855
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.