Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 486 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 587 |
| AccI | GTMKAC | 1 cut(s) | 1056 |
| AclWI | GGATC | 3 cut(s) | 336, 440, 455 |
| AcoI | YGGCCR | 2 cut(s) | 151, 717 |
| AcsI | RAATTY | 2 cut(s) | 234, 679 |
| AdeI | CACNNNGTG | 1 cut(s) | 486 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 560, 587 |
| AflIII | ACRYGT | 2 cut(s) | 775, 828 |
| AgsI | TTSAA | 8 cut(s) | 50, 119, 221, 650, 679, 916, 1022, 1166 |
| AjnI | CCWGG | 2 cut(s) | 386, 969 |
| Alw26I | GTCTC | 2 cut(s) | 819, 1161 |
| AlwI | GGATC | 3 cut(s) | 336, 440, 455 |
| AoxI | GGCC | 7 cut(s) | 151, 184, 551, 717, 723, 735, 1147 |
| ApeKI | GCWGC | 1 cut(s) | 569 |
| ApoI | RAATTY | 2 cut(s) | 234, 679 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 790, 822 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 933 |
| AspS9I | GGNCC | 1 cut(s) | 407 |
| AsuII | TTCGAA | 1 cut(s) | 203 |
| AvaII | GGWCC | 1 cut(s) | 407 |
| AxyI | CCTNAGG | 1 cut(s) | 1151 |
| BaeGI | GKGCMC | 1 cut(s) | 482 |
| BalI | TGGCCA | 2 cut(s) | 153, 719 |
| BanI | GGYRCC | 1 cut(s) | 486 |
| BbvI | GCAGC | 1 cut(s) | 556 |
| BccI | CCATC | 4 cut(s) | 332, 532, 997, 1045 |
| BciT130I | CCWGG | 2 cut(s) | 388, 971 |
| BclI | TGATCA | 1 cut(s) | 514 |
| BcoDI | GTCTC | 2 cut(s) | 819, 1161 |
| BfaI | CTAG | 1 cut(s) | 33 |
| BfmI | CTRYAG | 1 cut(s) | 987 |
| BisI | GCNGC | 1 cut(s) | 570 |
| BlpI | GCTNAGC | 1 cut(s) | 573 |
| BlsI | GCNGC | 1 cut(s) | 571 |
| Bme1390I | CCNGG | 2 cut(s) | 388, 971 |
| Bme18I | GGWCC | 1 cut(s) | 407 |
| BmgT120I | GGNCC | 1 cut(s) | 407 |
| BmiI | GGNNCC | 2 cut(s) | 488, 712 |
| BmrFI | CCNGG | 2 cut(s) | 388, 971 |
| BmsI | GCATC | 1 cut(s) | 135 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 605, 637 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 573 |
| Bpu14I | TTCGAA | 1 cut(s) | 203 |
| BpuEI | CTTGAG | 3 cut(s) | 39, 383, 851 |
| BsaI | GGTCTC | 1 cut(s) | 1161 |
| BsaJI | CCNNGG | 5 cut(s) | 148, 684, 714, 720, 732 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 945, 975 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 560, 587 |
| Bse1I | ACTGG | 2 cut(s) | 82, 449 |
| Bse21I | CCTNAGG | 1 cut(s) | 1151 |
| Bse3DI | GCAATG | 2 cut(s) | 615, 726 |
| BseBI | CCWGG | 2 cut(s) | 388, 971 |
| BseDI | CCNNGG | 5 cut(s) | 148, 684, 714, 720, 732 |
| BseGI | GGATG | 3 cut(s) | 126, 517, 966 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 560, 587 |
| BseMI | GCAATG | 2 cut(s) | 615, 726 |
| BseMII | CTCAG | 3 cut(s) | 587, 929, 1040 |
| BseNI | ACTGG | 2 cut(s) | 82, 449 |
| BseRI | GAGGAG | 3 cut(s) | 359, 386, 837 |
| BseSI | GKGCMC | 1 cut(s) | 482 |
| BseXI | GCAGC | 1 cut(s) | 556 |
| BseYI | CCCAGC | 1 cut(s) | 847 |
| BsgI | GTGCAG | 1 cut(s) | 132 |
| BshFI | GGCC | 7 cut(s) | 153, 186, 553, 719, 725, 737, 1149 |
| BshNI | GGYRCC | 1 cut(s) | 486 |
| BsiSI | CCGG | 1 cut(s) | 183 |
| BslFI | GGGAC | 1 cut(s) | 811 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 560, 587 |
| BsmAI | GTCTC | 2 cut(s) | 819, 1161 |
| BsmFI | GGGAC | 1 cut(s) | 811 |
| BsnI | GGCC | 7 cut(s) | 153, 186, 553, 719, 725, 737, 1149 |
| Bso31I | GGTCTC | 1 cut(s) | 1161 |
| Bsp119I | TTCGAA | 1 cut(s) | 203 |
| Bsp1286I | GDGCHC | 1 cut(s) | 482 |
| Bsp143I | GATC | 4 cut(s) | 328, 445, 460, 514 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 573 |
| Bsp19I | CCATGG | 2 cut(s) | 148, 714 |
| BspANI | GGCC | 7 cut(s) | 153, 186, 553, 719, 725, 737, 1149 |
| BspCNI | CTCAG | 3 cut(s) | 586, 930, 1039 |
| BspHI | TCATGA | 1 cut(s) | 511 |
| BspLI | GGNNCC | 2 cut(s) | 488, 712 |
| BspMAI | CTGCAG | 1 cut(s) | 991 |
| BspPI | GGATC | 3 cut(s) | 336, 440, 455 |
| BspT104I | TTCGAA | 1 cut(s) | 203 |
| BspT107I | GGYRCC | 1 cut(s) | 486 |
| BspTNI | GGTCTC | 1 cut(s) | 1161 |
| BsrDI | GCAATG | 2 cut(s) | 615, 726 |
| BsrI | ACTGG | 2 cut(s) | 82, 449 |
| BssECI | CCNNGG | 5 cut(s) | 148, 684, 714, 720, 732 |
| BssMI | GATC | 4 cut(s) | 328, 445, 460, 514 |
| BssT1I | CCWWGG | 5 cut(s) | 148, 684, 714, 720, 732 |
| Bst2UI | CCWGG | 2 cut(s) | 388, 971 |
| Bst4CI | ACNGT | 1 cut(s) | 760 |
| Bst6I | CTCTTC | 2 cut(s) | 645, 914 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 486 |
| BstBI | TTCGAA | 1 cut(s) | 203 |
| BstC8I | GCNNGC | 1 cut(s) | 633 |
| BstDEI | CTNAG | 4 cut(s) | 573, 938, 1026, 1151 |
| BstDSI | CCRYGG | 2 cut(s) | 148, 714 |
| BstF5I | GGATG | 3 cut(s) | 126, 517, 966 |
| BstKTI | GATC | 4 cut(s) | 331, 448, 463, 517 |
| BstMAI | GTCTC | 2 cut(s) | 819, 1161 |
| BstMBI | GATC | 4 cut(s) | 328, 445, 460, 514 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 277, 486, 614 |
| BstNI | CCWGG | 2 cut(s) | 388, 971 |
| BstNSI | RCATGY | 2 cut(s) | 779, 832 |
| BstSCI | CCNGG | 2 cut(s) | 386, 969 |
| BstSFI | CTRYAG | 1 cut(s) | 987 |
| BstSLI | GKGCMC | 1 cut(s) | 482 |
| BstV1I | GCAGC | 1 cut(s) | 556 |
| BstX2I | RGATCY | 2 cut(s) | 328, 460 |
| BstYI | RGATCY | 2 cut(s) | 328, 460 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1151 |
| BsuRI | GGCC | 7 cut(s) | 153, 186, 553, 719, 725, 737, 1149 |
| BtgI | CCRYGG | 2 cut(s) | 148, 714 |
| BtsCI | GGATG | 3 cut(s) | 126, 517, 966 |
| BtsI | GCAGTG | 1 cut(s) | 648 |
| BtsIMutI | CAGTG | 2 cut(s) | 648, 756 |
| Cac8I | GCNNGC | 1 cut(s) | 633 |
| CciI | TCATGA | 1 cut(s) | 511 |
| Cfr13I | GGNCC | 1 cut(s) | 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 624, 659 |
| DdeI | CTNAG | 4 cut(s) | 573, 938, 1026, 1151 |
| DpnI | GATC | 4 cut(s) | 330, 447, 462, 516 |
| DpnII | GATC | 4 cut(s) | 328, 445, 460, 514 |
| DraIII | CACNNNGTG | 1 cut(s) | 486 |
| EaeI | YGGCCR | 2 cut(s) | 151, 717 |
| Eam1104I | CTCTTC | 2 cut(s) | 645, 914 |
| EarI | CTCTTC | 2 cut(s) | 645, 914 |
| Eco130I | CCWWGG | 5 cut(s) | 148, 684, 714, 720, 732 |
| Eco147I | AGGCCT | 2 cut(s) | 553, 737 |
| Eco31I | GGTCTC | 1 cut(s) | 1161 |
| Eco47I | GGWCC | 1 cut(s) | 407 |
| Eco81I | CCTNAGG | 1 cut(s) | 1151 |
| EcoRI | GAATTC | 2 cut(s) | 234, 679 |
| EcoRII | CCWGG | 2 cut(s) | 386, 969 |
| EcoT14I | CCWWGG | 5 cut(s) | 148, 684, 714, 720, 732 |
| ErhI | CCWWGG | 5 cut(s) | 148, 684, 714, 720, 732 |
| FaqI | GGGAC | 1 cut(s) | 811 |
| FbaI | TGATCA | 1 cut(s) | 514 |
| FblI | GTMKAC | 1 cut(s) | 1056 |
| Fnu4HI | GCNGC | 1 cut(s) | 570 |
| FokI | GGATG | 3 cut(s) | 113, 504, 953 |
| Fsp4HI | GCNGC | 1 cut(s) | 570 |
| FspBI | CTAG | 1 cut(s) | 33 |
| GluI | GCNGC | 1 cut(s) | 570 |
| GsaI | CCCAGC | 1 cut(s) | 851 |
| HaeIII | GGCC | 7 cut(s) | 153, 186, 553, 719, 725, 737, 1149 |
| HapII | CCGG | 1 cut(s) | 183 |
| HincII | GTYRAC | 1 cut(s) | 1057 |
| HindII | GTYRAC | 1 cut(s) | 1057 |
| HinfI | GANTC | 7 cut(s) | 70, 139, 439, 620, 799, 874, 1116 |
| HpaII | CCGG | 1 cut(s) | 183 |
| Hpy166II | GTNNAC | 4 cut(s) | 196, 244, 1057, 1171 |
| Hpy188I | TCNGA | 3 cut(s) | 939, 1107, 1178 |
| Hpy188III | TCNNGA | 5 cut(s) | 326, 332, 443, 512, 742 |
| Hpy8I | GTNNAC | 4 cut(s) | 196, 244, 1057, 1171 |
| HpyAV | CCTTC | 5 cut(s) | 210, 398, 653, 748, 944 |
| HpyCH4III | ACNGT | 1 cut(s) | 760 |
| HpyCH4V | TGCA | 7 cut(s) | 113, 260, 756, 793, 857, 880, 989 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 277, 486, 614 |
| HpyF3I | CTNAG | 4 cut(s) | 573, 938, 1026, 1151 |
| Ksp22I | TGATCA | 1 cut(s) | 514 |
| Kzo9I | GATC | 4 cut(s) | 328, 445, 460, 514 |
| Lsp1109I | GCAGC | 1 cut(s) | 556 |
| LweI | GCATC | 1 cut(s) | 135 |
| MaeI | CTAG | 1 cut(s) | 33 |
| MaeIII | GTNAC | 1 cut(s) | 454 |
| MalI | GATC | 4 cut(s) | 330, 447, 462, 516 |
| MboI | GATC | 4 cut(s) | 328, 445, 460, 514 |
| MboII | GAAGA | 9 cut(s) | 38, 70, 281, 662, 850, 919, 928, 931, 1012 |
| MfeI | CAATTG | 2 cut(s) | 751, 1017 |
| MflI | RGATCY | 2 cut(s) | 328, 460 |
| MhlI | GDGCHC | 1 cut(s) | 482 |
| MlsI | TGGCCA | 2 cut(s) | 153, 719 |
| MluNI | TGGCCA | 2 cut(s) | 153, 719 |
| MlyI | GAGTC | 2 cut(s) | 614, 793 |
| MmeI | TCCRAC | 2 cut(s) | 676, 799 |
| Mox20I | TGGCCA | 2 cut(s) | 153, 719 |
| MroXI | GAANNNNTTC | 1 cut(s) | 933 |
| MscI | TGGCCA | 2 cut(s) | 153, 719 |
| MseI | TTAA | 3 cut(s) | 104, 413, 600 |
| MslI | CAYNNNNRTG | 3 cut(s) | 106, 147, 980 |
| Msp20I | TGGCCA | 2 cut(s) | 153, 719 |
| MspI | CCGG | 1 cut(s) | 183 |
| MspR9I | CCNGG | 2 cut(s) | 388, 971 |
| MunI | CAATTG | 2 cut(s) | 751, 1017 |
| MvaI | CCWGG | 2 cut(s) | 388, 971 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 277, 486, 614 |
| NcoI | CCATGG | 2 cut(s) | 148, 714 |
| NdeII | GATC | 4 cut(s) | 328, 445, 460, 514 |
| NlaIV | GGNNCC | 2 cut(s) | 488, 712 |
| NspI | RCATGY | 2 cut(s) | 779, 832 |
| NspV | TTCGAA | 1 cut(s) | 203 |
| PagI | TCATGA | 1 cut(s) | 511 |
| PceI | AGGCCT | 2 cut(s) | 553, 737 |
| PciI | ACATGT | 2 cut(s) | 775, 828 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 229 |
| PdmI | GAANNNNTTC | 1 cut(s) | 933 |
| PfeI | GAWTC | 5 cut(s) | 70, 139, 439, 874, 1116 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 587 |
| PfoI | TCCNGGA | 1 cut(s) | 969 |
| PkrI | GCNGC | 1 cut(s) | 571 |
| PleI | GAGTC | 2 cut(s) | 614, 793 |
| PpsI | GAGTC | 2 cut(s) | 614, 793 |
| PscI | ACATGT | 2 cut(s) | 775, 828 |
| Psp6I | CCWGG | 2 cut(s) | 386, 969 |
| PspFI | CCCAGC | 1 cut(s) | 847 |
| PspGI | CCWGG | 2 cut(s) | 386, 969 |
| PspN4I | GGNNCC | 2 cut(s) | 488, 712 |
| PspPI | GGNCC | 1 cut(s) | 407 |
| PstI | CTGCAG | 1 cut(s) | 991 |
| PsuI | RGATCY | 2 cut(s) | 328, 460 |
| RseI | CAYNNNNRTG | 3 cut(s) | 106, 147, 980 |
| SalI | GTCGAC | 1 cut(s) | 1055 |
| SaqAI | TTAA | 3 cut(s) | 104, 413, 600 |
| SatI | GCNGC | 1 cut(s) | 570 |
| Sau3AI | GATC | 4 cut(s) | 328, 445, 460, 514 |
| Sau96I | GGNCC | 1 cut(s) | 407 |
| SchI | GAGTC | 2 cut(s) | 614, 793 |
| ScrFI | CCNGG | 2 cut(s) | 388, 971 |
| SduI | GDGCHC | 1 cut(s) | 482 |
| SfaNI | GCATC | 1 cut(s) | 135 |
| SfcI | CTRYAG | 1 cut(s) | 987 |
| SfuI | TTCGAA | 1 cut(s) | 203 |
| SinI | GGWCC | 1 cut(s) | 407 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 106, 147, 980 |
| SmlI | CTYRAG | 3 cut(s) | 54, 398, 866 |
| SmoI | CTYRAG | 3 cut(s) | 54, 398, 866 |
| SseBI | AGGCCT | 2 cut(s) | 553, 737 |
| SspI | AATATT | 1 cut(s) | 316 |
| SspMI | CTAG | 1 cut(s) | 33 |
| StuI | AGGCCT | 2 cut(s) | 553, 737 |
| StyD4I | CCNGG | 2 cut(s) | 386, 969 |
| StyI | CCWWGG | 5 cut(s) | 148, 684, 714, 720, 732 |
| TaaI | ACNGT | 1 cut(s) | 760 |
| TaqI | TCGA | 2 cut(s) | 203, 1056 |
| TfiI | GAWTC | 5 cut(s) | 70, 139, 439, 874, 1116 |
| Tru1I | TTAA | 3 cut(s) | 104, 413, 600 |
| Tru9I | TTAA | 3 cut(s) | 104, 413, 600 |
| TscAI | CASTG | 2 cut(s) | 648, 763 |
| TseI | GCWGC | 1 cut(s) | 569 |
| TspDTI | ATGAA | 7 cut(s) | 76, 205, 311, 458, 509, 618, 963 |
| TspRI | CASTG | 2 cut(s) | 648, 763 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 587 |
| VpaK11BI | GGWCC | 1 cut(s) | 407 |
| XapI | RAATTY | 2 cut(s) | 234, 679 |
| XceI | RCATGY | 2 cut(s) | 779, 832 |
| XmiI | GTMKAC | 1 cut(s) | 1056 |
| XmnI | GAANNNNTTC | 1 cut(s) | 933 |
| XspI | CTAG | 1 cut(s) | 33 |