RchiOBHm_Chr2g0123081
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000692
Physical Location & Seq
Forward (+)
36210781 .. 36211422
642 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49540

Sequence Viewer

Length: 642 bp
ATGGAAAGACTGAATAGCTTTAAGCACCTTAACCATAAGTGGGCTAGTGAAGAGCATTCTACCCAGCTTCTTCAAGCTCAAGCCCACATATGGAACCACATTTTCAGCTTCATAAACTCCATGACCCTCAAATCTGCAATTGAACTAGGTATACCAGATATCATCAAAAAACATGGCCGCCCCATAACTCTTTCTGAGCTCACATCTGCATTACCAATTCACCCAACAAAAATCCCCAGTGTTTACCGCCTCATGCGTATATTGGTCCACTCCGGCATCTTTGCCAAAAAAAAGATGACTAAACTGGATGAGGAAGAAGGTTATATACTTACTGACGCTTCTCAGCTCCTCCTTAAGGATCACCCCTTCAGTATGACTCCATTTGTAATGGCTATGCTCAACCCTATCGTCACAAAACCATGGCATTATATGAACACTTGGTTCCAAAATAATGACCCTGCGGCATTTAACACGGCACATGGGATGACATTTTGGGACTACAGCACCCAGGAGCCAAGTCTTGCCAGCTTTTTCAATGATGCCATGGCTAGTGATACTCGTTTGGTGACCAGCGTGTTACTTCAGGAGTGCAAGGGGGTATTCGAGGGATTAAAATCATTAGTTGATGTTGGGGGTGGTTGA

Protein Analysis

213

Amino Acids

24.16

Weight (kDa)

6.83

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 31 - 120 4.1e-22 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 141 - 213 3.2e-11 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26800 FvH4_6g26810
malus_domestica MD01G1048000.v1.1 MD01G1048300.v1.1 MD01G1048400.v1.1 MD01G1051800.v1.1 MD01G1051900.v1.1 MD01G1149300.v1.1
prunus_persica Prupe.3G118900_v2.0.a1 Prupe.3G119100_v2.0.a1 Prupe.3G119700_v2.0.a1 Prupe.3G119800_v2.0.a1 Prupe.3G120200_v2.0.a1 Prupe.3G120400_v2.0.a1 Prupe.3G127600_v2.0.a1 Prupe.3G129000_v2.0.a1 Prupe.3G129200_v2.0.a1 Prupe.3G129400_v2.0.a1 Prupe.3G129600_v2.0.a1 Prupe.3G129800_v2.0.a1 Prupe.4G252900_v2.0.a1 Prupe.4G253200_v2.0.a1 Prupe.4G253300_v2.0.a1
pyrus_communis pycom01g07360 pycom01g07640 pycom01g07670 pycom01g07720 pycom01g07740 pycom01g07750
rosa_chinensis RchiOBHm_Chr2g0119291 RchiOBHm_Chr2g0123081 RchiOBHm_Chr2g0123091 RchiOBHm_Chr2g0128091 RchiOBHm_Chr2g0128121 RchiOBHm_Chr2g0128161 RchiOBHm_Chr4g0436611
rosa_laevigata RLG00000006507 RLG00000006508 RLG00000018994 RLG00000018995
rosa_multiflora Rmu_co8289337.1_g000001 Rmu_sc0000837.1_g000001 Rmu_sc0003130.1_g000011 Rmu_sc0027975.1_g000001
rosa_roxburghii Rroxscaffold_2G00115570 Rroxscaffold_2G00115600 Rroxscaffold_2G00116080 Rroxscaffold_2G00116090 Rroxscaffold_5G00377660
rosa_rugosa Rorug02G0278000 Rorug02G0278000 Rorug02G0278100 Rorug02G0558700 Rorug04G0294700
rosa_samantha Rh2AG330000 Rh2AG330300 Rh2AG330500 Rh2AG330600 Rh2AG330900 Rh2AG331100 Rh2BG310500 Rh2BG339200 Rh2BG339300 Rh2CG316800 Rh2DG304600 Rh2DG356000 Rh2DG356200 Rh4AG349100 Rh4BG357800 Rh4CG372300 Rh4DG352000 Rh5BG070000
rosa_wichuraiana Rw2G026730 Rw2G026760 Rw4G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 151
AciI CCGC 3 cut(s) 178, 247, 461
AclWI GGATC 1 cut(s) 366
AcoI YGGCCR 1 cut(s) 175
AcuI CTGAAG 2 cut(s) 352, 566
AfiI CCNNNNNNNGG 3 cut(s) 40, 90, 355
AflII CTTAAG 1 cut(s) 353
AgsI TTSAA 3 cut(s) 74, 143, 535
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 7 cut(s) 18, 67, 77, 108, 199, 346, 528
AluI AGCT 7 cut(s) 18, 67, 77, 108, 199, 346, 528
Alw21I GWGCWC 1 cut(s) 201
AlwI GGATC 1 cut(s) 366
AoxI GGCC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 3 cut(s) 212, 353, 577
AvaII GGWCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 201
BarI GAAGNNNNNNTAC 2 cut(s) 309, 341
Bbv12I GWGCWC 1 cut(s) 201
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 1 cut(s) 509
BfaI CTAG 3 cut(s) 45, 146, 549
BfmI CTRYAG 1 cut(s) 499
BfrI CTTAAG 1 cut(s) 353
BisI GCNGC 2 cut(s) 178, 462
BlsI GCNGC 2 cut(s) 179, 463
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 3 cut(s) 95, 443, 513
BmrFI CCNGG 1 cut(s) 509
BmrI ACTGGG 1 cut(s) 231
BmsI GCATC 2 cut(s) 285, 529
BmuI ACTGGG 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 63
BsaBI GATNNNNATC 1 cut(s) 613
BsaJI CCNNGG 3 cut(s) 419, 507, 543
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 90, 355
Bse1I ACTGG 2 cut(s) 237, 309
Bse8I GATNNNNATC 1 cut(s) 613
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 3 cut(s) 419, 507, 543
BseGI GGATG 2 cut(s) 313, 489
BseJI GATNNNNATC 1 cut(s) 613
BseLI CCNNNNNNNGG 3 cut(s) 40, 90, 355
BseMII CTCAG 2 cut(s) 186, 356
BseNI ACTGG 2 cut(s) 237, 309
BseRI GAGGAG 1 cut(s) 338
BseYI CCCAGC 1 cut(s) 63
BshFI GGCC 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 3 cut(s) 40, 90, 355
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 1 cut(s) 358
Bsp19I CCATGG 2 cut(s) 419, 543
BspACI CCGC 3 cut(s) 178, 247, 461
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 2 cut(s) 187, 355
BspLI GGNNCC 3 cut(s) 95, 443, 513
BspPI GGATC 1 cut(s) 366
BspQI GCTCTTC 1 cut(s) 45
BspTI CTTAAG 1 cut(s) 353
BsrI ACTGG 2 cut(s) 237, 309
BssECI CCNNGG 3 cut(s) 419, 507, 543
BssMI GATC 1 cut(s) 358
BssNAI GTATAC 1 cut(s) 152
BssT1I CCWWGG 2 cut(s) 419, 543
Bst1107I GTATAC 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 509
Bst6I CTCTTC 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 2 cut(s) 195, 342
BstDSI CCRYGG 2 cut(s) 419, 543
BstEII GGTNACC 1 cut(s) 565
BstENI CCTNNNNNAGG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 313, 489
BstKTI GATC 1 cut(s) 361
BstMBI GATC 1 cut(s) 358
BstNI CCWGG 1 cut(s) 509
BstPI GGTNACC 1 cut(s) 565
BstSCI CCNGG 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 499
BstZ17I GTATAC 1 cut(s) 152
BsuRI GGCC 1 cut(s) 177
BtgI CCRYGG 2 cut(s) 419, 543
BtsCI GGATG 2 cut(s) 313, 489
BtsIMutI CAGTG 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 1 cut(s) 344
CviAII CATG 6 cut(s) 121, 173, 253, 420, 479, 544
DdeI CTNAG 2 cut(s) 195, 342
DpnI GATC 1 cut(s) 360
DpnII GATC 1 cut(s) 358
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 419, 543
Eco24I GRGCYC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 2 cut(s) 352, 566
Eco91I GGTNACC 1 cut(s) 565
EcoICRI GAGCTC 1 cut(s) 199
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoO65I GGTNACC 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 507
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 2 cut(s) 419, 543
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 2 cut(s) 419, 543
FaeI CATG 6 cut(s) 124, 176, 256, 423, 482, 547
FaqI GGGAC 1 cut(s) 509
FatI CATG 6 cut(s) 120, 172, 252, 419, 478, 543
FauNDI CATATG 1 cut(s) 89
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 2 cut(s) 178, 462
FokI GGATG 2 cut(s) 320, 496
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 2 cut(s) 178, 462
FspBI CTAG 3 cut(s) 45, 146, 549
GluI GCNGC 2 cut(s) 178, 462
GsaI CCCAGC 1 cut(s) 67
HaeIII GGCC 1 cut(s) 177
HapII CCGG 1 cut(s) 273
HgaI GACGC 1 cut(s) 344
Hin1II CATG 6 cut(s) 124, 176, 256, 423, 482, 547
HinfI GANTC 1 cut(s) 376
HpaII CCGG 1 cut(s) 273
HphI GGTGA 3 cut(s) 212, 353, 577
Hpy166II GTNNAC 3 cut(s) 152, 244, 268
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 1 cut(s) 584
Hpy8I GTNNAC 3 cut(s) 152, 244, 268
HpyAV CCTTC 2 cut(s) 311, 376
HpyCH4V TGCA 3 cut(s) 137, 209, 591
HpyF3I CTNAG 2 cut(s) 195, 342
Hsp92II CATG 6 cut(s) 124, 176, 256, 423, 482, 547
Kzo9I GATC 1 cut(s) 358
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 2 cut(s) 351, 511
LweI GCATC 2 cut(s) 285, 529
MaeI CTAG 3 cut(s) 45, 146, 549
MaeIII GTNAC 3 cut(s) 409, 565, 576
MalI GATC 1 cut(s) 360
MboI GATC 1 cut(s) 358
MboII GAAGA 3 cut(s) 62, 62, 326
MfeI CAATTG 1 cut(s) 138
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 2 cut(s) 138, 216
MlyI GAGTC 1 cut(s) 370
MnlI CCTC 5 cut(s) 137, 260, 304, 359, 598
MseI TTAA 5 cut(s) 21, 30, 354, 468, 611
MspCI CTTAAG 1 cut(s) 353
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 1 cut(s) 509
MunI CAATTG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 1 cut(s) 509
NcoI CCATGG 2 cut(s) 419, 543
NdeI CATATG 1 cut(s) 89
NdeII GATC 1 cut(s) 358
NlaIII CATG 6 cut(s) 124, 176, 256, 423, 482, 547
NlaIV GGNNCC 3 cut(s) 95, 443, 513
NmuCI GTSAC 2 cut(s) 409, 565
PciSI GCTCTTC 1 cut(s) 45
PctI GAATGC 1 cut(s) 55
PkrI GCNGC 2 cut(s) 179, 463
PleI GAGTC 1 cut(s) 370
PpsI GAGTC 1 cut(s) 370
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 507
PspEI GGTNACC 1 cut(s) 565
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 3 cut(s) 95, 443, 513
PspPI GGNCC 1 cut(s) 265
PsrI GAACNNNNNNTAC 2 cut(s) 135, 167
SacI GAGCTC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 5 cut(s) 21, 30, 354, 468, 611
SatI GCNGC 2 cut(s) 178, 462
Sau3AI GATC 1 cut(s) 358
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 1 cut(s) 201
SfaNI GCATC 2 cut(s) 285, 529
SfcI CTRYAG 1 cut(s) 499
SinI GGWCC 1 cut(s) 265
SmlI CTYRAG 2 cut(s) 78, 353
SmoI CTYRAG 2 cut(s) 78, 353
Sse9I AATT 2 cut(s) 138, 216
SsiI CCGC 3 cut(s) 178, 247, 461
SspMI CTAG 3 cut(s) 45, 146, 549
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 2 cut(s) 419, 543
TaqI TCGA 1 cut(s) 603
TasI AATT 2 cut(s) 138, 216
TauI GCSGC 2 cut(s) 180, 464
Tru1I TTAA 5 cut(s) 21, 30, 354, 468, 611
Tru9I TTAA 5 cut(s) 21, 30, 354, 468, 611
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 2 cut(s) 409, 565
Tsp45I GTSAC 2 cut(s) 409, 565
TspDTI ATGAA 2 cut(s) 100, 446
TspRI CASTG 1 cut(s) 244
Vha464I CTTAAG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 151
XspI CTAG 3 cut(s) 45, 146, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.