Prupe.4G253300_v2.0.a1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000692
Physical Location & Seq
Forward (+)
17235902 .. 17237801
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G253300.1

Sequence Viewer

Length: 1071 bp
ATGGATTTGAGTAACGAAATGAGTTCTGCCAACCTACTCCAAGCTCAAGCCCACATTTGGAATTGCATTTTCAGTTTCATAAACCCTTTGTCTCTCAAATGTGCAGTTCAACTAGGTATACCAGACATCATAAAGAAACATGGCAATCCCATGAGCCTTTCTGAGCTTATATCTGCATTGCCAATCCACCCAACAAAATCCAATTGTGTCTACCGGCTCATGCGAATCTTAGTGCACTCGGGCTTCTTCGGTCGCCAAAAGCTTAGTGAACTTGATGAGGAAGAAGGTTATGTGCTGACGGATGCTTCCAGGCTCCTTCTTAAGGACGATCCCTTAAGCGCAAGACCCTTCTTACTTGGCCAGCTTGATCCTTTTATGACCAAACCATGGCATTACTTTAGCACTTGGTTCCAAAATAATGACCCTACAGCATGTTTCACGGCGCATGGGACAACATTTTGGGATTTAGGGTACCTCGAGCCAAGTCTTTCCCACATTTTCAATGATTCCATGGCTAGTGATGCTCGACTAATCTCGAAGGTGGTGAGTAATGAGTACAAGGGGGTGTTTGAGGGATTGGAGTCCTTGGTTGATGTTGGGGGTGGTATAGGAACCATGGCCAAGGCCATTGCTGATGTATTTCCCCATGTGGAATGCATTGTATTTGATCTCCCACATGTGGTCGCTGACCTAAAAGGGAGTAAGAACTTGAAATTTTTTGGAGGGGACATGTTTGAGGCCATTCCTCACACAGATGCAATTTTAATGAAGTGGATATTGCATGACTGGTCCGATGAAGAATGTATAAAAATACTTCAGCGATCTAAAGAGGCAATTACAAGGAAGGAAAAGAAAGGCAAGGTGATTATCATTGACATGGTAATGAAGCAGAAGGGAGATGACCAATCAATTGAGACGCAGCTCTTCTTCGATATGTTGATGATGGTGTTGGTTACAGGAAAAGAAAGGACTGAGAAAGAATGGGCTAAGCTATTTTCTGATGCAGGTTTCAGTGACTATAAGATAACTCCCATTTGCGGTTTAAGATATCTAATTGAGGTTTATCCTTGA

Protein Analysis

357

Amino Acids

40.25

Weight (kDa)

5.58

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26800 FvH4_6g26810
malus_domestica MD01G1048000.v1.1 MD01G1048300.v1.1 MD01G1048400.v1.1 MD01G1051800.v1.1 MD01G1051900.v1.1 MD01G1149300.v1.1
prunus_persica Prupe.3G118900_v2.0.a1 Prupe.3G119100_v2.0.a1 Prupe.3G119700_v2.0.a1 Prupe.3G119800_v2.0.a1 Prupe.3G120200_v2.0.a1 Prupe.3G120400_v2.0.a1 Prupe.3G127600_v2.0.a1 Prupe.3G129000_v2.0.a1 Prupe.3G129200_v2.0.a1 Prupe.3G129400_v2.0.a1 Prupe.3G129600_v2.0.a1 Prupe.3G129800_v2.0.a1 Prupe.4G252900_v2.0.a1 Prupe.4G253200_v2.0.a1 Prupe.4G253300_v2.0.a1
pyrus_communis pycom01g07360 pycom01g07640 pycom01g07670 pycom01g07720 pycom01g07740 pycom01g07750
rosa_chinensis RchiOBHm_Chr2g0119291 RchiOBHm_Chr2g0123081 RchiOBHm_Chr2g0123091 RchiOBHm_Chr2g0128091 RchiOBHm_Chr2g0128121 RchiOBHm_Chr2g0128161 RchiOBHm_Chr4g0436611
rosa_laevigata RLG00000006507 RLG00000006508 RLG00000018994 RLG00000018995
rosa_multiflora Rmu_co8289337.1_g000001 Rmu_sc0000837.1_g000001 Rmu_sc0003130.1_g000011 Rmu_sc0027975.1_g000001
rosa_roxburghii Rroxscaffold_2G00115570 Rroxscaffold_2G00115600 Rroxscaffold_2G00116080 Rroxscaffold_2G00116090 Rroxscaffold_5G00377660
rosa_rugosa Rorug02G0278000 Rorug02G0278000 Rorug02G0278100 Rorug02G0558700 Rorug04G0294700
rosa_samantha Rh2AG330000 Rh2AG330300 Rh2AG330500 Rh2AG330600 Rh2AG330900 Rh2AG331100 Rh2BG310500 Rh2BG339200 Rh2BG339300 Rh2CG316800 Rh2DG304600 Rh2DG356000 Rh2DG356200 Rh4AG349100 Rh4BG357800 Rh4CG372300 Rh4DG352000 Rh5BG070000
rosa_wichuraiana Rw2G026730 Rw2G026760 Rw4G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 995
Acc65I GGTACC 1 cut(s) 471
AccB1I GGYRCC 1 cut(s) 471
AccB7I CCANNNNNTGG 1 cut(s) 387
AccI GTMKAC 2 cut(s) 118, 210
AciI CCGC 1 cut(s) 1038
AclWI GGATC 2 cut(s) 323, 362
AcoI YGGCCR 2 cut(s) 358, 618
AcsI RAATTY 1 cut(s) 713
AcuI CTGAAG 1 cut(s) 800
AfaI GTAC 2 cut(s) 473, 557
AfiI CCNNNNNNNGG 5 cut(s) 57, 322, 387, 679, 1037
AflII CTTAAG 2 cut(s) 320, 334
AflIII ACRYGT 2 cut(s) 676, 729
AgsI TTSAA 3 cut(s) 110, 502, 712
AjnI CCWGG 1 cut(s) 308
AluBI AGCT 6 cut(s) 44, 166, 262, 364, 922, 991
AluI AGCT 6 cut(s) 44, 166, 262, 364, 922, 991
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 2 cut(s) 96, 908
Alw44I GTGCAC 1 cut(s) 233
AlwI GGATC 2 cut(s) 323, 362
Ama87I CYCGRG 2 cut(s) 238, 476
AoxI GGCC 4 cut(s) 358, 618, 624, 738
ApaLI GTGCAC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 919
ApoI RAATTY 1 cut(s) 713
Asp718I GGTACC 1 cut(s) 471
AspLEI GCGC 2 cut(s) 341, 445
AspS9I GGNCC 1 cut(s) 789
AsuHPI GGTGA 2 cut(s) 556, 874
AvaI CYCGRG 2 cut(s) 238, 476
AvaII GGWCC 1 cut(s) 789
BaeGI GKGCMC 1 cut(s) 237
BalI TGGCCA 2 cut(s) 360, 620
BanI GGYRCC 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 931
BccI CCATC 1 cut(s) 937
BceAI ACGGC 1 cut(s) 456
BciT130I CCWGG 1 cut(s) 310
BcoDI GTCTC 2 cut(s) 96, 908
BfaI CTAG 2 cut(s) 113, 516
BfmI CTRYAG 1 cut(s) 426
BfrI CTTAAG 2 cut(s) 320, 334
BfuAI ACCTGC 1 cut(s) 995
BisI GCNGC 1 cut(s) 920
BlpI GCTNAGC 1 cut(s) 987
BlsI GCNGC 1 cut(s) 921
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 1 cut(s) 789
BmeT110I CYCGRG 2 cut(s) 238, 476
BmgT120I GGNCC 1 cut(s) 789
BmiI GGNNCC 4 cut(s) 314, 410, 473, 613
BmrFI CCNGG 1 cut(s) 310
BmsI GCATC 4 cut(s) 292, 511, 745, 991
Bpu1102I GCTNAGC 1 cut(s) 987
BpuEI CTTGAG 1 cut(s) 30
BsaJI CCNNGG 5 cut(s) 386, 510, 585, 615, 621
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 322, 387, 679, 1037
Bse118I RCCGGY 1 cut(s) 213
Bse1I ACTGG 1 cut(s) 791
Bse3DI GCAATG 2 cut(s) 176, 627
BseBI CCWGG 1 cut(s) 310
BseDI CCNNGG 5 cut(s) 386, 510, 585, 615, 621
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 5 cut(s) 57, 322, 387, 679, 1037
BseMI GCAATG 2 cut(s) 176, 627
BseMII CTCAG 2 cut(s) 153, 963
BseNI ACTGG 1 cut(s) 791
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 1 cut(s) 931
BsgI GTGCAG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 4 cut(s) 360, 620, 626, 740
BshNI GGYRCC 1 cut(s) 471
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 2 cut(s) 238, 476
BsiSI CCGG 1 cut(s) 214
BslFI GGGAC 2 cut(s) 463, 740
BslI CCNNNNNNNGG 5 cut(s) 57, 322, 387, 679, 1037
BsmAI GTCTC 2 cut(s) 96, 908
BsmBI CGTCTC 1 cut(s) 908
BsmFI GGGAC 2 cut(s) 463, 740
BsmI GAATGC 1 cut(s) 659
BsnI GGCC 4 cut(s) 360, 620, 626, 740
BsoBI CYCGRG 2 cut(s) 238, 476
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 4 cut(s) 328, 367, 667, 821
Bsp1720I GCTNAGC 1 cut(s) 987
Bsp19I CCATGG 3 cut(s) 386, 510, 615
BspACI CCGC 1 cut(s) 1038
BspANI GGCC 4 cut(s) 360, 620, 626, 740
BspCNI CTCAG 2 cut(s) 154, 964
BspLI GGNNCC 4 cut(s) 314, 410, 473, 613
BspMI ACCTGC 1 cut(s) 995
BspPI GGATC 2 cut(s) 323, 362
BspQI GCTCTTC 1 cut(s) 929
BspT107I GGYRCC 1 cut(s) 471
BspTI CTTAAG 2 cut(s) 320, 334
BsrDI GCAATG 2 cut(s) 176, 627
BsrFI RCCGGY 1 cut(s) 213
BsrI ACTGG 1 cut(s) 791
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 5 cut(s) 386, 510, 585, 615, 621
BssMI GATC 4 cut(s) 328, 367, 667, 821
BssNAI GTATAC 1 cut(s) 119
BssT1I CCWWGG 5 cut(s) 386, 510, 585, 615, 621
Bst1107I GTATAC 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 310
Bst6I CTCTTC 1 cut(s) 929
BstAFI CTTAAG 2 cut(s) 320, 334
BstC8I GCNNGC 1 cut(s) 362
BstDEI CTNAG 5 cut(s) 162, 229, 263, 972, 987
BstDSI CCRYGG 3 cut(s) 386, 510, 615
BstENI CCTNNNNNAGG 1 cut(s) 320
BstF5I GGATG 1 cut(s) 307
BstHHI GCGC 2 cut(s) 341, 445
BstKTI GATC 4 cut(s) 331, 370, 670, 824
BstMAI GTCTC 2 cut(s) 96, 908
BstMBI GATC 4 cut(s) 328, 367, 667, 821
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 1 cut(s) 521
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 3 cut(s) 435, 680, 733
BstSCI CCNGG 1 cut(s) 308
BstSFI CTRYAG 1 cut(s) 426
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 931
BstZ17I GTATAC 1 cut(s) 119
BsuRI GGCC 4 cut(s) 360, 620, 626, 740
BtgI CCRYGG 3 cut(s) 386, 510, 615
BtsCI GGATG 1 cut(s) 307
BtsIMutI CAGTG 1 cut(s) 1018
BveI ACCTGC 1 cut(s) 995
Cac8I GCNNGC 1 cut(s) 362
CfoI GCGC 2 cut(s) 341, 445
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 789
CseI GACGC 1 cut(s) 925
Csp6I GTAC 2 cut(s) 472, 556
CviQI GTAC 2 cut(s) 472, 556
DdeI CTNAG 5 cut(s) 162, 229, 263, 972, 987
DpnI GATC 4 cut(s) 330, 369, 669, 823
DpnII GATC 4 cut(s) 328, 367, 667, 821
EaeI YGGCCR 2 cut(s) 358, 618
Eam1104I CTCTTC 1 cut(s) 929
EarI CTCTTC 1 cut(s) 929
Eco130I CCWWGG 5 cut(s) 386, 510, 585, 615, 621
Eco32I GATATC 1 cut(s) 1049
Eco47I GGWCC 1 cut(s) 789
Eco57I CTGAAG 1 cut(s) 800
Eco88I CYCGRG 2 cut(s) 238, 476
EcoNI CCTNNNNNAGG 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 308
EcoRV GATATC 1 cut(s) 1049
EcoT14I CCWWGG 5 cut(s) 386, 510, 585, 615, 621
EcoT22I ATGCAT 1 cut(s) 659
ErhI CCWWGG 5 cut(s) 386, 510, 585, 615, 621
Esp3I CGTCTC 1 cut(s) 908
FaqI GGGAC 2 cut(s) 463, 740
FblI GTMKAC 2 cut(s) 118, 210
Fnu4HI GCNGC 1 cut(s) 920
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 920
FspBI CTAG 2 cut(s) 113, 516
GlaI GCGC 2 cut(s) 340, 444
GluI GCNGC 1 cut(s) 920
HaeIII GGCC 4 cut(s) 360, 620, 626, 740
HapII CCGG 1 cut(s) 214
HgaI GACGC 1 cut(s) 925
HhaI GCGC 2 cut(s) 341, 445
Hin6I GCGC 2 cut(s) 339, 443
HinP1I GCGC 2 cut(s) 339, 443
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 3 cut(s) 225, 506, 581
HpaII CCGG 1 cut(s) 214
HphI GGTGA 2 cut(s) 556, 874
Hpy166II GTNNAC 4 cut(s) 119, 211, 235, 269
Hpy188I TCNGA 3 cut(s) 163, 793, 1000
Hpy188III TCNNGA 1 cut(s) 535
Hpy8I GTNNAC 4 cut(s) 119, 211, 235, 269
HpyAV CCTTC 6 cut(s) 278, 326, 358, 532, 838, 886
HpyCH4V TGCA 8 cut(s) 66, 104, 176, 235, 657, 758, 781, 1004
HpyF10VI GCNNNNNNNGC 1 cut(s) 521
HpyF3I CTNAG 5 cut(s) 162, 229, 263, 972, 987
HspAI GCGC 2 cut(s) 339, 443
KpnI GGTACC 1 cut(s) 475
Kzo9I GATC 4 cut(s) 328, 367, 667, 821
LguI GCTCTTC 1 cut(s) 929
LmnI GCTCC 1 cut(s) 318
LpnPI CCDG 8 cut(s) 135, 227, 295, 322, 374, 772, 942, 990
Lsp1109I GCAGC 1 cut(s) 931
LweI GCATC 4 cut(s) 292, 511, 745, 991
MaeI CTAG 2 cut(s) 113, 516
MaeIII GTNAC 3 cut(s) 11, 952, 1013
MalI GATC 4 cut(s) 330, 369, 669, 823
MboI GATC 4 cut(s) 328, 367, 667, 821
MboII GAAGA 5 cut(s) 238, 293, 809, 916, 919
MfeI CAATTG 2 cut(s) 202, 909
MhlI GDGCHC 1 cut(s) 237
MlsI TGGCCA 2 cut(s) 360, 620
MluCI AATT 7 cut(s) 61, 202, 713, 759, 834, 909, 1053
MluNI TGGCCA 2 cut(s) 360, 620
MlyI GAGTC 1 cut(s) 590
MnlI CCTC 8 cut(s) 271, 485, 565, 716, 730, 756, 823, 1051
Mox20I TGGCCA 2 cut(s) 360, 620
Mph1103I ATGCAT 1 cut(s) 659
MscI TGGCCA 2 cut(s) 360, 620
MseI TTAA 4 cut(s) 321, 335, 764, 1043
MslI CAYNNNNRTG 3 cut(s) 753, 875, 881
Msp20I TGGCCA 2 cut(s) 360, 620
MspCI CTTAAG 2 cut(s) 320, 334
MspI CCGG 1 cut(s) 214
MspR9I CCNGG 1 cut(s) 310
MunI CAATTG 2 cut(s) 202, 909
Mva1269I GAATGC 1 cut(s) 659
MvaI CCWGG 1 cut(s) 310
MwoI GCNNNNNNNGC 1 cut(s) 521
NcoI CCATGG 3 cut(s) 386, 510, 615
NdeII GATC 4 cut(s) 328, 367, 667, 821
NlaIV GGNNCC 4 cut(s) 314, 410, 473, 613
NmuCI GTSAC 1 cut(s) 1013
NsiI ATGCAT 1 cut(s) 659
NspI RCATGY 3 cut(s) 435, 680, 733
PaeR7I CTCGAG 1 cut(s) 476
PciI ACATGT 2 cut(s) 676, 729
PciSI GCTCTTC 1 cut(s) 929
PctI GAATGC 1 cut(s) 659
PfeI GAWTC 2 cut(s) 225, 506
PflMI CCANNNNNTGG 1 cut(s) 387
PkrI GCNGC 1 cut(s) 921
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
PscI ACATGT 2 cut(s) 676, 729
Psp6I CCWGG 1 cut(s) 308
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 4 cut(s) 314, 410, 473, 613
PspPI GGNCC 1 cut(s) 789
PspXI VCTCGAGB 1 cut(s) 476
RsaI GTAC 2 cut(s) 473, 557
RsaNI GTAC 2 cut(s) 472, 556
RseI CAYNNNNRTG 3 cut(s) 753, 875, 881
SapI GCTCTTC 1 cut(s) 929
SaqAI TTAA 4 cut(s) 321, 335, 764, 1043
SatI GCNGC 1 cut(s) 920
Sau3AI GATC 4 cut(s) 328, 367, 667, 821
Sau96I GGNCC 1 cut(s) 789
SchI GAGTC 1 cut(s) 590
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 4 cut(s) 292, 511, 745, 991
SfcI CTRYAG 1 cut(s) 426
Sfr274I CTCGAG 1 cut(s) 476
SinI GGWCC 1 cut(s) 789
SlaI CTCGAG 1 cut(s) 476
SmiMI CAYNNNNRTG 3 cut(s) 753, 875, 881
SmlI CTYRAG 4 cut(s) 45, 320, 334, 476
SmoI CTYRAG 4 cut(s) 45, 320, 334, 476
Sse9I AATT 7 cut(s) 61, 202, 713, 759, 834, 909, 1053
SsiI CCGC 1 cut(s) 1038
SspMI CTAG 2 cut(s) 113, 516
StyD4I CCNGG 1 cut(s) 308
StyI CCWWGG 5 cut(s) 386, 510, 585, 615, 621
TaqI TCGA 4 cut(s) 477, 526, 536, 930
TaqII GACCGA 1 cut(s) 239
TasI AATT 7 cut(s) 61, 202, 713, 759, 834, 909, 1053
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 2 cut(s) 225, 506
Tru1I TTAA 4 cut(s) 321, 335, 764, 1043
Tru9I TTAA 4 cut(s) 321, 335, 764, 1043
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 1 cut(s) 1013
TseI GCWGC 1 cut(s) 919
Tsp45I GTSAC 1 cut(s) 1013
TspDTI ATGAA 4 cut(s) 67, 782, 810, 899
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 1 cut(s) 1018
Van91I CCANNNNNTGG 1 cut(s) 387
Vha464I CTTAAG 2 cut(s) 320, 334
VneI GTGCAC 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 789
XagI CCTNNNNNAGG 1 cut(s) 320
XapI RAATTY 1 cut(s) 713
XceI RCATGY 3 cut(s) 435, 680, 733
XhoI CTCGAG 1 cut(s) 476
XmiI GTMKAC 2 cut(s) 118, 210
XspI CTAG 2 cut(s) 113, 516
Zsp2I ATGCAT 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.