Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1104 |
| Acc16I | TGCGCA | 1 cut(s) | 233 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 766 |
| AciI | CCGC | 1 cut(s) | 404 |
| AclWI | GGATC | 2 cut(s) | 458, 525 |
| AcoI | YGGCCR | 2 cut(s) | 151, 672 |
| AfaI | GTAC | 3 cut(s) | 387, 408, 481 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 542, 766 |
| AflIII | ACRYGT | 4 cut(s) | 75, 730, 786, 955 |
| AgsI | TTSAA | 5 cut(s) | 491, 634, 874, 998, 1142 |
| AjiI | CACGTC | 1 cut(s) | 136 |
| AjnI | CCWGG | 1 cut(s) | 639 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 496, 528 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 524, 556 |
| Alw21I | GWGCWC | 2 cut(s) | 55, 211 |
| Alw26I | GTCTC | 1 cut(s) | 777 |
| AlwI | GGATC | 2 cut(s) | 458, 525 |
| AoxI | GGCC | 4 cut(s) | 151, 362, 672, 678 |
| ApeKI | GCWGC | 3 cut(s) | 113, 524, 1085 |
| AspLEI | GCGC | 2 cut(s) | 187, 234 |
| AspS9I | GGNCC | 1 cut(s) | 362 |
| AsuHPI | GGTGA | 2 cut(s) | 29, 470 |
| BalI | TGGCCA | 1 cut(s) | 674 |
| BanII | GRGCYC | 2 cut(s) | 55, 211 |
| Bbv12I | GWGCWC | 2 cut(s) | 55, 211 |
| BbvI | GCAGC | 3 cut(s) | 125, 536, 1072 |
| BccI | CCATC | 4 cut(s) | 167, 373, 424, 970 |
| BceAI | ACGGC | 1 cut(s) | 166 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 239, 273 |
| BciT130I | CCWGG | 1 cut(s) | 641 |
| BcoDI | GTCTC | 1 cut(s) | 777 |
| BfaI | CTAG | 4 cut(s) | 33, 122, 342, 1089 |
| BglII | AGATCT | 2 cut(s) | 1074, 1130 |
| BisI | GCNGC | 3 cut(s) | 114, 525, 1086 |
| BlsI | GCNGC | 3 cut(s) | 115, 526, 1087 |
| Bme1390I | CCNGG | 1 cut(s) | 641 |
| BmgBI | CACGTC | 1 cut(s) | 136 |
| BmgT120I | GGNCC | 1 cut(s) | 362 |
| BmiI | GGNNCC | 4 cut(s) | 71, 534, 667, 754 |
| BmrFI | CCNGG | 1 cut(s) | 641 |
| BmsI | GCATC | 5 cut(s) | 135, 219, 243, 391, 711 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 560, 592 |
| Bpu10I | CCTNAGC | 1 cut(s) | 528 |
| BpuEI | CTTGAG | 2 cut(s) | 39, 809 |
| BsaBI | GATNNNNATC | 2 cut(s) | 233, 932 |
| BsaJI | CCNNGG | 6 cut(s) | 96, 148, 536, 639, 669, 675 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 542, 766 |
| Bse1I | ACTGG | 2 cut(s) | 82, 1045 |
| Bse3DI | GCAATG | 2 cut(s) | 570, 681 |
| Bse8I | GATNNNNATC | 2 cut(s) | 233, 932 |
| BseBI | CCWGG | 1 cut(s) | 641 |
| BseDI | CCNNGG | 6 cut(s) | 96, 148, 536, 639, 669, 675 |
| BseGI | GGATG | 3 cut(s) | 126, 234, 652 |
| BseJI | GATNNNNATC | 2 cut(s) | 233, 932 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 542, 766 |
| BseMI | GCAATG | 2 cut(s) | 570, 681 |
| BseMII | CTCAG | 3 cut(s) | 74, 542, 890 |
| BseNI | ACTGG | 2 cut(s) | 82, 1045 |
| BseRI | GAGGAG | 2 cut(s) | 630, 669 |
| BseXI | GCAGC | 3 cut(s) | 125, 536, 1072 |
| BseYI | CCCAGC | 2 cut(s) | 698, 805 |
| BsgI | GTGCAG | 1 cut(s) | 132 |
| BshFI | GGCC | 4 cut(s) | 153, 364, 674, 680 |
| BsiHKAI | GWGCWC | 2 cut(s) | 55, 211 |
| BslFI | GGGAC | 1 cut(s) | 966 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 542, 766 |
| BsmAI | GTCTC | 1 cut(s) | 777 |
| BsmFI | GGGAC | 1 cut(s) | 966 |
| BsmI | GAATGC | 2 cut(s) | 510, 692 |
| BsnI | GGCC | 4 cut(s) | 153, 364, 674, 680 |
| Bsp1286I | GDGCHC | 2 cut(s) | 55, 211 |
| Bsp1407I | TGTACA | 1 cut(s) | 479 |
| Bsp143I | GATC | 5 cut(s) | 172, 463, 517, 1074, 1130 |
| Bsp19I | CCATGG | 2 cut(s) | 96, 669 |
| BspACI | CCGC | 1 cut(s) | 404 |
| BspANI | GGCC | 4 cut(s) | 153, 364, 674, 680 |
| BspCNI | CTCAG | 3 cut(s) | 73, 541, 891 |
| BspHI | TCATGA | 2 cut(s) | 280, 390 |
| BspLI | GGNNCC | 4 cut(s) | 71, 534, 667, 754 |
| BspPI | GGATC | 2 cut(s) | 458, 525 |
| BsrDI | GCAATG | 2 cut(s) | 570, 681 |
| BsrGI | TGTACA | 1 cut(s) | 479 |
| BsrI | ACTGG | 2 cut(s) | 82, 1045 |
| BssECI | CCNNGG | 6 cut(s) | 96, 148, 536, 639, 669, 675 |
| BssMI | GATC | 5 cut(s) | 172, 463, 517, 1074, 1130 |
| BssT1I | CCWWGG | 4 cut(s) | 96, 536, 669, 675 |
| Bst2UI | CCWGG | 1 cut(s) | 641 |
| Bst4CI | ACNGT | 1 cut(s) | 455 |
| Bst6I | CTCTTC | 1 cut(s) | 600 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 708 |
| BstAUI | TGTACA | 1 cut(s) | 479 |
| BstDEI | CTNAG | 3 cut(s) | 60, 528, 899 |
| BstDSI | CCRYGG | 3 cut(s) | 96, 148, 669 |
| BstF5I | GGATG | 3 cut(s) | 126, 234, 652 |
| BstHHI | GCGC | 2 cut(s) | 187, 234 |
| BstKTI | GATC | 5 cut(s) | 175, 466, 520, 1077, 1133 |
| BstMAI | GTCTC | 1 cut(s) | 777 |
| BstMBI | GATC | 5 cut(s) | 172, 463, 517, 1074, 1130 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 231, 569, 708 |
| BstNI | CCWGG | 1 cut(s) | 641 |
| BstNSI | RCATGY | 4 cut(s) | 79, 734, 790, 959 |
| BstSCI | CCNGG | 1 cut(s) | 639 |
| BstV1I | GCAGC | 3 cut(s) | 125, 536, 1072 |
| BstX2I | RGATCY | 3 cut(s) | 517, 1074, 1130 |
| BstYI | RGATCY | 3 cut(s) | 517, 1074, 1130 |
| BsuRI | GGCC | 4 cut(s) | 153, 364, 674, 680 |
| BtgI | CCRYGG | 3 cut(s) | 96, 148, 669 |
| BtgZI | GCGATG | 1 cut(s) | 864 |
| BtrI | CACGTC | 1 cut(s) | 136 |
| BtsCI | GGATG | 3 cut(s) | 126, 234, 652 |
| BtsI | GCAGTG | 1 cut(s) | 180 |
| BtsIMutI | CAGTG | 2 cut(s) | 180, 1038 |
| CciI | TCATGA | 2 cut(s) | 280, 390 |
| CfoI | GCGC | 2 cut(s) | 187, 234 |
| Cfr13I | GGNCC | 1 cut(s) | 362 |
| Csp6I | GTAC | 3 cut(s) | 386, 407, 480 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 364, 399 |
| CviQI | GTAC | 3 cut(s) | 386, 407, 480 |
| DdeI | CTNAG | 3 cut(s) | 60, 528, 899 |
| DpnI | GATC | 5 cut(s) | 174, 465, 519, 1076, 1132 |
| DpnII | GATC | 5 cut(s) | 172, 463, 517, 1074, 1130 |
| EaeI | YGGCCR | 2 cut(s) | 151, 672 |
| Eam1104I | CTCTTC | 1 cut(s) | 600 |
| EarI | CTCTTC | 1 cut(s) | 600 |
| Ecl136II | GAGCTC | 2 cut(s) | 53, 209 |
| Eco130I | CCWWGG | 4 cut(s) | 96, 536, 669, 675 |
| Eco24I | GRGCYC | 2 cut(s) | 55, 211 |
| Eco53kI | GAGCTC | 2 cut(s) | 53, 209 |
| EcoICRI | GAGCTC | 2 cut(s) | 53, 209 |
| EcoRII | CCWGG | 1 cut(s) | 639 |
| EcoT14I | CCWWGG | 4 cut(s) | 96, 536, 669, 675 |
| EcoT22I | ATGCAT | 1 cut(s) | 694 |
| EcoT38I | GRGCYC | 2 cut(s) | 55, 211 |
| ErhI | CCWWGG | 4 cut(s) | 96, 536, 669, 675 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 879, 911 |
| FaqI | GGGAC | 1 cut(s) | 966 |
| Fnu4HI | GCNGC | 3 cut(s) | 114, 525, 1086 |
| FokI | GGATG | 3 cut(s) | 113, 221, 659 |
| FriOI | GRGCYC | 2 cut(s) | 55, 211 |
| Fsp4HI | GCNGC | 3 cut(s) | 114, 525, 1086 |
| FspAI | RTGCGCAY | 1 cut(s) | 233 |
| FspBI | CTAG | 4 cut(s) | 33, 122, 342, 1089 |
| FspI | TGCGCA | 1 cut(s) | 233 |
| GlaI | GCGC | 2 cut(s) | 186, 233 |
| GluI | GCNGC | 3 cut(s) | 114, 525, 1086 |
| GsaI | CCCAGC | 2 cut(s) | 702, 809 |
| HaeIII | GGCC | 4 cut(s) | 153, 364, 674, 680 |
| HhaI | GCGC | 2 cut(s) | 187, 234 |
| Hin6I | GCGC | 2 cut(s) | 185, 232 |
| HinP1I | GCGC | 2 cut(s) | 185, 232 |
| HindIII | AAGCTT | 1 cut(s) | 581 |
| HinfI | GANTC | 4 cut(s) | 139, 575, 832, 1058 |
| HphI | GGTGA | 2 cut(s) | 29, 470 |
| Hpy166II | GTNNAC | 3 cut(s) | 196, 244, 482 |
| Hpy188I | TCNGA | 7 cut(s) | 63, 172, 250, 590, 900, 1063, 1130 |
| Hpy188III | TCNNGA | 4 cut(s) | 204, 281, 391, 530 |
| Hpy8I | GTNNAC | 3 cut(s) | 196, 244, 482 |
| HpyAV | CCTTC | 4 cut(s) | 210, 585, 908, 1045 |
| HpyCH4III | ACNGT | 1 cut(s) | 455 |
| HpyCH4IV | ACGT | 2 cut(s) | 135, 1118 |
| HpyCH4V | TGCA | 8 cut(s) | 113, 260, 445, 692, 711, 748, 815, 838 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 231, 569, 708 |
| HpyF3I | CTNAG | 3 cut(s) | 60, 528, 899 |
| HpySE526I | ACGT | 2 cut(s) | 135, 1118 |
| HspAI | GCGC | 2 cut(s) | 185, 232 |
| Kzo9I | GATC | 5 cut(s) | 172, 463, 517, 1074, 1130 |
| LmnI | GCTCC | 4 cut(s) | 50, 434, 665, 846 |
| Lsp1109I | GCAGC | 3 cut(s) | 125, 536, 1072 |
| LweI | GCATC | 5 cut(s) | 135, 219, 243, 391, 711 |
| MaeI | CTAG | 4 cut(s) | 33, 122, 342, 1089 |
| MaeII | ACGT | 2 cut(s) | 135, 1118 |
| MaeIII | GTNAC | 2 cut(s) | 287, 545 |
| MalI | GATC | 5 cut(s) | 174, 465, 519, 1076, 1132 |
| MboI | GATC | 5 cut(s) | 172, 463, 517, 1074, 1130 |
| MboII | GAAGA | 9 cut(s) | 314, 317, 341, 617, 866, 877, 886, 937, 985 |
| MfeI | CAATTG | 1 cut(s) | 993 |
| MflI | RGATCY | 3 cut(s) | 517, 1074, 1130 |
| MhlI | GDGCHC | 2 cut(s) | 55, 211 |
| MlsI | TGGCCA | 1 cut(s) | 674 |
| MluCI | AATT | 3 cut(s) | 763, 816, 993 |
| MluNI | TGGCCA | 1 cut(s) | 674 |
| MlyI | GAGTC | 1 cut(s) | 569 |
| MmeI | TCCRAC | 2 cut(s) | 359, 631 |
| MnlI | CCTC | 8 cut(s) | 248, 292, 562, 589, 608, 619, 647, 650 |
| Mox20I | TGGCCA | 1 cut(s) | 674 |
| Mph1103I | ATGCAT | 1 cut(s) | 694 |
| MscI | TGGCCA | 1 cut(s) | 674 |
| MslI | CAYNNNNRTG | 5 cut(s) | 106, 270, 285, 791, 947 |
| Msp20I | TGGCCA | 1 cut(s) | 674 |
| MspR9I | CCNGG | 1 cut(s) | 641 |
| MunI | CAATTG | 1 cut(s) | 993 |
| Mva1269I | GAATGC | 2 cut(s) | 510, 692 |
| MvaI | CCWGG | 1 cut(s) | 641 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 231, 569, 708 |
| NcoI | CCATGG | 2 cut(s) | 96, 669 |
| NdeII | GATC | 5 cut(s) | 172, 463, 517, 1074, 1130 |
| NlaIV | GGNNCC | 4 cut(s) | 71, 534, 667, 754 |
| NmuCI | GTSAC | 1 cut(s) | 287 |
| NsbI | TGCGCA | 1 cut(s) | 233 |
| NsiI | ATGCAT | 1 cut(s) | 694 |
| NspI | RCATGY | 4 cut(s) | 79, 734, 790, 959 |
| PagI | TCATGA | 2 cut(s) | 280, 390 |
| PciI | ACATGT | 4 cut(s) | 75, 730, 786, 955 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 246 |
| PctI | GAATGC | 2 cut(s) | 510, 692 |
| PfeI | GAWTC | 3 cut(s) | 139, 832, 1058 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 766 |
| PkrI | GCNGC | 3 cut(s) | 115, 526, 1087 |
| PleI | GAGTC | 1 cut(s) | 569 |
| PpsI | GAGTC | 1 cut(s) | 569 |
| PscI | ACATGT | 4 cut(s) | 75, 730, 786, 955 |
| PsiI | TTATAA | 1 cut(s) | 1104 |
| Psp124BI | GAGCTC | 2 cut(s) | 55, 211 |
| Psp6I | CCWGG | 1 cut(s) | 639 |
| PspFI | CCCAGC | 2 cut(s) | 698, 805 |
| PspGI | CCWGG | 1 cut(s) | 639 |
| PspN4I | GGNNCC | 4 cut(s) | 71, 534, 667, 754 |
| PspPI | GGNCC | 1 cut(s) | 362 |
| PsuI | RGATCY | 3 cut(s) | 517, 1074, 1130 |
| RsaI | GTAC | 3 cut(s) | 387, 408, 481 |
| RsaNI | GTAC | 3 cut(s) | 386, 407, 480 |
| RseI | CAYNNNNRTG | 5 cut(s) | 106, 270, 285, 791, 947 |
| SacI | GAGCTC | 2 cut(s) | 55, 211 |
| SatI | GCNGC | 3 cut(s) | 114, 525, 1086 |
| Sau3AI | GATC | 5 cut(s) | 172, 463, 517, 1074, 1130 |
| Sau96I | GGNCC | 1 cut(s) | 362 |
| SchI | GAGTC | 1 cut(s) | 569 |
| ScrFI | CCNGG | 1 cut(s) | 641 |
| SduI | GDGCHC | 2 cut(s) | 55, 211 |
| SfaNI | GCATC | 5 cut(s) | 135, 219, 243, 391, 711 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 106, 270, 285, 791, 947 |
| SmlI | CTYRAG | 2 cut(s) | 54, 824 |
| SmoI | CTYRAG | 2 cut(s) | 54, 824 |
| Sse9I | AATT | 3 cut(s) | 763, 816, 993 |
| SsiI | CCGC | 1 cut(s) | 404 |
| SspMI | CTAG | 4 cut(s) | 33, 122, 342, 1089 |
| SstI | GAGCTC | 2 cut(s) | 55, 211 |
| StyD4I | CCNGG | 1 cut(s) | 639 |
| StyI | CCWWGG | 4 cut(s) | 96, 536, 669, 675 |
| TaaI | ACNGT | 1 cut(s) | 455 |
| TaiI | ACGT | 2 cut(s) | 138, 1121 |
| TaqI | TCGA | 3 cut(s) | 203, 399, 960 |
| TasI | AATT | 3 cut(s) | 763, 816, 993 |
| TatI | WGTACW | 2 cut(s) | 385, 479 |
| TfiI | GAWTC | 3 cut(s) | 139, 832, 1058 |
| TscAI | CASTG | 2 cut(s) | 187, 1045 |
| TseFI | GTSAC | 1 cut(s) | 287 |
| TseI | GCWGC | 3 cut(s) | 113, 524, 1085 |
| Tsp45I | GTSAC | 1 cut(s) | 287 |
| TspDTI | ATGAA | 7 cut(s) | 76, 205, 266, 464, 573, 867, 927 |
| TspRI | CASTG | 2 cut(s) | 187, 1045 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 766 |
| XceI | RCATGY | 4 cut(s) | 79, 734, 790, 959 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 44, 763 |
| XspI | CTAG | 4 cut(s) | 33, 122, 342, 1089 |
| Zsp2I | ATGCAT | 1 cut(s) | 694 |