Rroxscaffold_2G00116080
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000692
Physical Location & Seq
Reverse (-)
42833972 .. 42835250
1279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116080.1

Sequence Viewer

Length: 666 bp
ATGACCCTTAAATCTGCAATTGAACTAGGTATACCAGATATCATCAAAAAACATGGCCGCCCCATAACTCTTTCTGAGCTCACATCTGCATTACCAATTCACCCAACAAAAGTCCTCAGTGTTTACCGCCTCATGCGTATATTGGTCCACTCCGGCTTCTTTGCAAAAAAAGAGCTGAATGAACTGGATGAGCAAGAAAGTTATATACTTACTGACGCTTCTCAGCTCCTCCTTAAGGATCACCCCTTCAGTATAACACCATTTCTAATGGCTGTGCTCAACCCTAACTTCACAAACACATGCCATTATATGAGCACTTGGTTCCAAAATGATGACCCTACTCCATTTAACACGGCATATGGGATGACGTTTTGGGACTACATCACCCAGGAGCCAAGTCATGCCAACCTTTTCAATGATGCCATGGCTAGTGATACTCGAACAATGGCCAAGGCCATTGTTGATGCATTCCCAGAAATTAACTGCACCGTACTTGATCTCCCACATGTGGTGGCTGACCTGCAAGGAAGTAAGAACCTGAAATATGCTGGAGGGGACATGTTTGAGGCAGTTCCTCCGGCAGATGCAATTATGTTGAAGGTTTTCAGTGACTATAAGATAACTCCCAGTGTGGGTGTAAGATCTCTCATTGAGGTTTATCCTTGA

Protein Analysis

221

Amino Acids

24.76

Weight (kDa)

5.27

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 1 - 80 3e-17 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 149 - 204 5.7e-11 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26800 FvH4_6g26810
malus_domestica MD01G1048000.v1.1 MD01G1048300.v1.1 MD01G1048400.v1.1 MD01G1051800.v1.1 MD01G1051900.v1.1 MD01G1149300.v1.1
prunus_persica Prupe.3G118900_v2.0.a1 Prupe.3G119100_v2.0.a1 Prupe.3G119700_v2.0.a1 Prupe.3G119800_v2.0.a1 Prupe.3G120200_v2.0.a1 Prupe.3G120400_v2.0.a1 Prupe.3G127600_v2.0.a1 Prupe.3G129000_v2.0.a1 Prupe.3G129200_v2.0.a1 Prupe.3G129400_v2.0.a1 Prupe.3G129600_v2.0.a1 Prupe.3G129800_v2.0.a1 Prupe.4G252900_v2.0.a1 Prupe.4G253200_v2.0.a1 Prupe.4G253300_v2.0.a1
pyrus_communis pycom01g07360 pycom01g07640 pycom01g07670 pycom01g07720 pycom01g07740 pycom01g07750
rosa_chinensis RchiOBHm_Chr2g0119291 RchiOBHm_Chr2g0123081 RchiOBHm_Chr2g0123091 RchiOBHm_Chr2g0128091 RchiOBHm_Chr2g0128121 RchiOBHm_Chr2g0128161 RchiOBHm_Chr4g0436611
rosa_laevigata RLG00000006507 RLG00000006508 RLG00000018994 RLG00000018995
rosa_multiflora Rmu_co8289337.1_g000001 Rmu_sc0000837.1_g000001 Rmu_sc0003130.1_g000011 Rmu_sc0027975.1_g000001
rosa_roxburghii Rroxscaffold_2G00115570 Rroxscaffold_2G00115600 Rroxscaffold_2G00116080 Rroxscaffold_2G00116090 Rroxscaffold_5G00377660
rosa_rugosa Rorug02G0278000 Rorug02G0278000 Rorug02G0278100 Rorug02G0558700 Rorug04G0294700
rosa_samantha Rh2AG330000 Rh2AG330300 Rh2AG330500 Rh2AG330600 Rh2AG330900 Rh2AG331100 Rh2BG310500 Rh2BG339200 Rh2BG339300 Rh2CG316800 Rh2DG304600 Rh2DG356000 Rh2DG356200 Rh4AG349100 Rh4BG357800 Rh4CG372300 Rh4DG352000 Rh5BG070000
rosa_wichuraiana Rw2G026730 Rw2G026760 Rw4G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 528
AccI GTMKAC 1 cut(s) 31
AciI CCGC 2 cut(s) 58, 127
AclWI GGATC 1 cut(s) 246
AcoI YGGCCR 2 cut(s) 55, 447
AcuI CTGAAG 1 cut(s) 232
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 3 cut(s) 235, 508, 632
AflII CTTAAG 1 cut(s) 233
AflIII ACRYGT 2 cut(s) 505, 558
AgsI TTSAA 3 cut(s) 23, 415, 598
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 3 cut(s) 79, 175, 226
AluI AGCT 3 cut(s) 79, 175, 226
Alw21I GWGCWC 3 cut(s) 81, 279, 317
AlwI GGATC 1 cut(s) 246
AoxI GGCC 3 cut(s) 55, 447, 453
Asp700I GAANNNNTTC 1 cut(s) 602
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 3 cut(s) 92, 233, 376
AvaII GGWCC 1 cut(s) 145
BalI TGGCCA 1 cut(s) 449
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 3 cut(s) 81, 279, 317
BceAI ACGGC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 389
BfaI CTAG 2 cut(s) 26, 429
BfrI CTTAAG 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 528
BglII AGATCT 1 cut(s) 641
BisI GCNGC 1 cut(s) 58
BlsI GCNGC 1 cut(s) 59
Bme1390I CCNGG 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 2 cut(s) 323, 393
BmrFI CCNGG 1 cut(s) 389
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 3 cut(s) 409, 454, 574
BmuI ACTGGG 1 cut(s) 621
BpmI CTGGAG 1 cut(s) 570
BsaJI CCNNGG 3 cut(s) 387, 423, 450
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 508, 632
Bse1I ACTGG 2 cut(s) 189, 627
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 3 cut(s) 387, 423, 450
BseGI GGATG 2 cut(s) 193, 369
BseLI CCNNNNNNNGG 3 cut(s) 235, 508, 632
BseMII CTCAG 3 cut(s) 66, 130, 236
BseNI ACTGG 2 cut(s) 189, 627
BseRI GAGGAG 1 cut(s) 218
BsgI GTGCAG 1 cut(s) 469
BshFI GGCC 3 cut(s) 57, 449, 455
BsiHKAI GWGCWC 3 cut(s) 81, 279, 317
BsiSI CCGG 2 cut(s) 153, 578
BslFI GGGAC 2 cut(s) 389, 569
BslI CCNNNNNNNGG 3 cut(s) 235, 508, 632
BsmFI GGGAC 2 cut(s) 389, 569
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 3 cut(s) 57, 449, 455
Bsp1286I GDGCHC 3 cut(s) 81, 279, 317
Bsp143I GATC 3 cut(s) 238, 496, 641
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 2 cut(s) 58, 127
BspANI GGCC 3 cut(s) 57, 449, 455
BspCNI CTCAG 3 cut(s) 67, 129, 235
BspLI GGNNCC 2 cut(s) 323, 393
BspMI ACCTGC 1 cut(s) 528
BspPI GGATC 1 cut(s) 246
BspTI CTTAAG 1 cut(s) 233
BsrI ACTGG 2 cut(s) 189, 627
BssECI CCNNGG 3 cut(s) 387, 423, 450
BssMI GATC 3 cut(s) 238, 496, 641
BssNAI GTATAC 1 cut(s) 32
BssT1I CCWWGG 2 cut(s) 423, 450
Bst1107I GTATAC 1 cut(s) 32
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 490
BstAFI CTTAAG 1 cut(s) 233
BstDEI CTNAG 3 cut(s) 75, 116, 222
BstDSI CCRYGG 1 cut(s) 423
BstENI CCTNNNNNAGG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 193, 369
BstKTI GATC 3 cut(s) 241, 499, 644
BstMBI GATC 3 cut(s) 238, 496, 641
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 3 cut(s) 303, 509, 562
BstSCI CCNGG 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 641
BstYI RGATCY 1 cut(s) 641
BstZ17I GTATAC 1 cut(s) 32
BsuRI GGCC 3 cut(s) 57, 449, 455
BtgI CCRYGG 1 cut(s) 423
BtsCI GGATG 2 cut(s) 193, 369
BtsIMutI CAGTG 3 cut(s) 124, 613, 634
BveI ACCTGC 1 cut(s) 528
Cfr13I GGNCC 1 cut(s) 145
CseI GACGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 491
CviAII CATG 7 cut(s) 53, 133, 300, 401, 424, 506, 559
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 3 cut(s) 75, 116, 222
DpnI GATC 3 cut(s) 240, 498, 643
DpnII GATC 3 cut(s) 238, 496, 641
EaeI YGGCCR 2 cut(s) 55, 447
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 2 cut(s) 423, 450
Eco24I GRGCYC 1 cut(s) 81
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 145
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 79
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 387
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 2 cut(s) 423, 450
EcoT22I ATGCAT 1 cut(s) 469
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 2 cut(s) 423, 450
FaeI CATG 7 cut(s) 56, 136, 303, 404, 427, 509, 562
FaqI GGGAC 2 cut(s) 389, 569
FatI CATG 7 cut(s) 52, 132, 299, 400, 423, 505, 558
FauNDI CATATG 1 cut(s) 358
FblI GTMKAC 1 cut(s) 31
Fnu4HI GCNGC 1 cut(s) 58
FokI GGATG 2 cut(s) 200, 376
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 1 cut(s) 58
FspBI CTAG 2 cut(s) 26, 429
GluI GCNGC 1 cut(s) 58
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 3 cut(s) 57, 449, 455
HapII CCGG 2 cut(s) 153, 578
HgaI GACGC 1 cut(s) 224
Hin1II CATG 7 cut(s) 56, 136, 303, 404, 427, 509, 562
HpaII CCGG 2 cut(s) 153, 578
HphI GGTGA 3 cut(s) 92, 233, 376
Hpy166II GTNNAC 3 cut(s) 32, 124, 148
Hpy188I TCNGA 1 cut(s) 76
Hpy8I GTNNAC 3 cut(s) 32, 124, 148
HpyAV CCTTC 2 cut(s) 256, 592
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 7 cut(s) 17, 89, 164, 467, 486, 523, 587
HpyF3I CTNAG 3 cut(s) 75, 116, 222
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 7 cut(s) 56, 136, 303, 404, 427, 509, 562
Kzo9I GATC 3 cut(s) 238, 496, 641
LmnI GCTCC 2 cut(s) 231, 391
LweI GCATC 3 cut(s) 409, 454, 574
MaeI CTAG 2 cut(s) 26, 429
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 1 cut(s) 608
MalI GATC 3 cut(s) 240, 498, 643
MboI GATC 3 cut(s) 238, 496, 641
MfeI CAATTG 1 cut(s) 18
MflI RGATCY 1 cut(s) 641
MhlI GDGCHC 3 cut(s) 81, 279, 317
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 4 cut(s) 18, 96, 477, 588
MluNI TGGCCA 1 cut(s) 449
MnlI CCTC 7 cut(s) 125, 140, 239, 545, 559, 585, 646
Mox20I TGGCCA 1 cut(s) 449
Mph1103I ATGCAT 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 602
MscI TGGCCA 1 cut(s) 449
MseI TTAA 4 cut(s) 9, 234, 348, 480
Msp20I TGGCCA 1 cut(s) 449
MspCI CTTAAG 1 cut(s) 233
MspI CCGG 2 cut(s) 153, 578
MspR9I CCNGG 1 cut(s) 389
MunI CAATTG 1 cut(s) 18
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 1 cut(s) 389
NcoI CCATGG 1 cut(s) 423
NdeI CATATG 1 cut(s) 358
NdeII GATC 3 cut(s) 238, 496, 641
NlaIII CATG 7 cut(s) 56, 136, 303, 404, 427, 509, 562
NlaIV GGNNCC 2 cut(s) 323, 393
NmuCI GTSAC 1 cut(s) 608
NsiI ATGCAT 1 cut(s) 469
NspI RCATGY 3 cut(s) 303, 509, 562
PciI ACATGT 2 cut(s) 505, 558
PctI GAATGC 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 602
PkrI GCNGC 1 cut(s) 59
PscI ACATGT 2 cut(s) 505, 558
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 2 cut(s) 323, 393
PspPI GGNCC 1 cut(s) 145
PsrI GAACNNNNNNTAC 2 cut(s) 15, 47
PsuI RGATCY 1 cut(s) 641
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 4 cut(s) 9, 234, 348, 480
SatI GCNGC 1 cut(s) 58
Sau3AI GATC 3 cut(s) 238, 496, 641
Sau96I GGNCC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 3 cut(s) 81, 279, 317
SfaNI GCATC 3 cut(s) 409, 454, 574
SinI GGWCC 1 cut(s) 145
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 4 cut(s) 18, 96, 477, 588
SsiI CCGC 2 cut(s) 58, 127
SspMI CTAG 2 cut(s) 26, 429
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 1 cut(s) 387
StyI CCWWGG 2 cut(s) 423, 450
TaaI ACNGT 1 cut(s) 490
TaiI ACGT 1 cut(s) 371
TaqI TCGA 1 cut(s) 439
TasI AATT 4 cut(s) 18, 96, 477, 588
TauI GCSGC 1 cut(s) 60
Tru1I TTAA 4 cut(s) 9, 234, 348, 480
Tru9I TTAA 4 cut(s) 9, 234, 348, 480
TscAI CASTG 3 cut(s) 124, 613, 634
TseFI GTSAC 1 cut(s) 608
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 1 cut(s) 195
TspRI CASTG 3 cut(s) 124, 613, 634
Vha464I CTTAAG 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 145
XagI CCTNNNNNAGG 1 cut(s) 233
XceI RCATGY 3 cut(s) 303, 509, 562
XmiI GTMKAC 1 cut(s) 31
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 2 cut(s) 26, 429
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.