Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 230 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 763 |
| AclWI | GGATC | 3 cut(s) | 392, 455, 664 |
| AcoI | YGGCCR | 3 cut(s) | 148, 177, 669 |
| AfaI | GTAC | 1 cut(s) | 478 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 539, 763 |
| AflII | CTTAAG | 1 cut(s) | 821 |
| AflIII | ACRYGT | 3 cut(s) | 473, 727, 783 |
| AgsI | TTSAA | 7 cut(s) | 116, 442, 488, 631, 871, 995, 1139 |
| AjiI | CACGTC | 1 cut(s) | 133 |
| AjnI | CCWGG | 2 cut(s) | 338, 1036 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 493, 525 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 521, 553 |
| Alw21I | GWGCWC | 2 cut(s) | 52, 174 |
| Alw26I | GTCTC | 3 cut(s) | 220, 774, 1134 |
| AlwI | GGATC | 3 cut(s) | 392, 455, 664 |
| AoxI | GGCC | 4 cut(s) | 148, 177, 669, 675 |
| ApeKI | GCWGC | 2 cut(s) | 467, 1082 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 745, 777 |
| AspLEI | GCGC | 1 cut(s) | 231 |
| AspS9I | GGNCC | 1 cut(s) | 190 |
| AsuC2I | CCSGG | 1 cut(s) | 181 |
| AsuHPI | GGTGA | 1 cut(s) | 467 |
| AvaII | GGWCC | 1 cut(s) | 190 |
| BalI | TGGCCA | 1 cut(s) | 671 |
| BanII | GRGCYC | 3 cut(s) | 52, 174, 186 |
| Bbv12I | GWGCWC | 2 cut(s) | 52, 174 |
| BbvI | GCAGC | 2 cut(s) | 454, 1069 |
| BccI | CCATC | 4 cut(s) | 164, 421, 970, 1037 |
| BceAI | ACGGC | 1 cut(s) | 163 |
| BciT130I | CCWGG | 2 cut(s) | 340, 1038 |
| BcnI | CCSGG | 1 cut(s) | 181 |
| BcoDI | GTCTC | 3 cut(s) | 220, 774, 1134 |
| BfaI | CTAG | 3 cut(s) | 30, 119, 525 |
| BfrI | CTTAAG | 1 cut(s) | 821 |
| BglII | AGATCT | 1 cut(s) | 1071 |
| BisI | GCNGC | 2 cut(s) | 468, 1083 |
| BlpI | GCTNAGC | 1 cut(s) | 57 |
| BlsI | GCNGC | 2 cut(s) | 469, 1084 |
| Bme1390I | CCNGG | 3 cut(s) | 181, 340, 1038 |
| Bme18I | GGWCC | 1 cut(s) | 190 |
| BmgBI | CACGTC | 1 cut(s) | 133 |
| BmgT120I | GGNCC | 1 cut(s) | 190 |
| BmiI | GGNNCC | 2 cut(s) | 68, 531 |
| BmrFI | CCNGG | 3 cut(s) | 181, 340, 1038 |
| BmsI | GCATC | 3 cut(s) | 132, 240, 799 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 557, 589 |
| BpmI | CTGGAG | 1 cut(s) | 786 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 57 |
| BpuEI | CTTGAG | 2 cut(s) | 36, 170 |
| BpuMI | CCSGG | 1 cut(s) | 181 |
| BsaBI | GATNNNNATC | 2 cut(s) | 929, 1028 |
| BsaI | GGTCTC | 1 cut(s) | 1134 |
| BsaJI | CCNNGG | 4 cut(s) | 145, 533, 672, 1036 |
| BsaWI | WCCGGW | 1 cut(s) | 400 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 539, 763 |
| Bse1I | ACTGG | 1 cut(s) | 79 |
| Bse3DI | GCAATG | 2 cut(s) | 567, 678 |
| Bse8I | GATNNNNATC | 2 cut(s) | 929, 1028 |
| BseBI | CCWGG | 2 cut(s) | 340, 1038 |
| BseDI | CCNNGG | 4 cut(s) | 145, 533, 672, 1036 |
| BseGI | GGATG | 3 cut(s) | 231, 649, 1029 |
| BseJI | GATNNNNATC | 2 cut(s) | 929, 1028 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 539, 763 |
| BseMI | GCAATG | 2 cut(s) | 567, 678 |
| BseMII | CTCAG | 2 cut(s) | 71, 1013 |
| BseNI | ACTGG | 1 cut(s) | 79 |
| BseRI | GAGGAG | 2 cut(s) | 627, 792 |
| BseXI | GCAGC | 2 cut(s) | 454, 1069 |
| BsgI | GTGCAG | 2 cut(s) | 129, 691 |
| BshFI | GGCC | 4 cut(s) | 150, 179, 671, 677 |
| BsiHKAI | GWGCWC | 2 cut(s) | 52, 174 |
| BsiSI | CCGG | 2 cut(s) | 180, 401 |
| BslFI | GGGAC | 2 cut(s) | 619, 763 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 539, 763 |
| BsmAI | GTCTC | 3 cut(s) | 220, 774, 1134 |
| BsmFI | GGGAC | 2 cut(s) | 619, 763 |
| BsmI | GAATGC | 2 cut(s) | 507, 689 |
| BsnI | GGCC | 4 cut(s) | 150, 179, 671, 677 |
| Bso31I | GGTCTC | 1 cut(s) | 1134 |
| Bsp1286I | GDGCHC | 3 cut(s) | 52, 174, 186 |
| Bsp1407I | TGTACA | 1 cut(s) | 476 |
| Bsp143I | GATC | 5 cut(s) | 280, 397, 460, 656, 1071 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 57 |
| BspANI | GGCC | 4 cut(s) | 150, 179, 671, 677 |
| BspCNI | CTCAG | 2 cut(s) | 70, 1012 |
| BspHI | TCATGA | 2 cut(s) | 277, 387 |
| BspLI | GGNNCC | 2 cut(s) | 68, 531 |
| BspPI | GGATC | 3 cut(s) | 392, 455, 664 |
| BspTI | CTTAAG | 1 cut(s) | 821 |
| BspTNI | GGTCTC | 1 cut(s) | 1134 |
| BsrDI | GCAATG | 2 cut(s) | 567, 678 |
| BsrGI | TGTACA | 1 cut(s) | 476 |
| BsrI | ACTGG | 1 cut(s) | 79 |
| BssECI | CCNNGG | 4 cut(s) | 145, 533, 672, 1036 |
| BssMI | GATC | 5 cut(s) | 280, 397, 460, 656, 1071 |
| BssT1I | CCWWGG | 2 cut(s) | 533, 672 |
| Bst2UI | CCWGG | 2 cut(s) | 340, 1038 |
| Bst4CI | ACNGT | 3 cut(s) | 310, 452, 712 |
| Bst6I | CTCTTC | 1 cut(s) | 597 |
| BstAFI | CTTAAG | 1 cut(s) | 821 |
| BstAUI | TGTACA | 1 cut(s) | 476 |
| BstC8I | GCNNGC | 2 cut(s) | 739, 743 |
| BstDEI | CTNAG | 3 cut(s) | 57, 896, 999 |
| BstDSI | CCRYGG | 1 cut(s) | 145 |
| BstF5I | GGATG | 3 cut(s) | 231, 649, 1029 |
| BstHHI | GCGC | 1 cut(s) | 231 |
| BstKTI | GATC | 5 cut(s) | 283, 400, 463, 659, 1074 |
| BstMAI | GTCTC | 3 cut(s) | 220, 774, 1134 |
| BstMBI | GATC | 5 cut(s) | 280, 397, 460, 656, 1071 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 228, 566 |
| BstNI | CCWGG | 2 cut(s) | 340, 1038 |
| BstNSI | RCATGY | 3 cut(s) | 477, 731, 787 |
| BstSCI | CCNGG | 3 cut(s) | 179, 338, 1036 |
| BstV1I | GCAGC | 2 cut(s) | 454, 1069 |
| BstX2I | RGATCY | 1 cut(s) | 1071 |
| BstYI | RGATCY | 1 cut(s) | 1071 |
| BsuRI | GGCC | 4 cut(s) | 150, 179, 671, 677 |
| BtgI | CCRYGG | 1 cut(s) | 145 |
| BtgZI | GCGATG | 1 cut(s) | 109 |
| BtrI | CACGTC | 1 cut(s) | 133 |
| BtsCI | GGATG | 3 cut(s) | 231, 649, 1029 |
| BtsIMutI | CAGTG | 1 cut(s) | 708 |
| Cac8I | GCNNGC | 2 cut(s) | 739, 743 |
| CciI | TCATGA | 2 cut(s) | 277, 387 |
| CfoI | GCGC | 1 cut(s) | 231 |
| Cfr13I | GGNCC | 1 cut(s) | 190 |
| Csp6I | GTAC | 1 cut(s) | 477 |
| CviQI | GTAC | 1 cut(s) | 477 |
| DdeI | CTNAG | 3 cut(s) | 57, 896, 999 |
| DpnI | GATC | 5 cut(s) | 282, 399, 462, 658, 1073 |
| DpnII | GATC | 5 cut(s) | 280, 397, 460, 656, 1071 |
| EaeI | YGGCCR | 3 cut(s) | 148, 177, 669 |
| Eam1104I | CTCTTC | 1 cut(s) | 597 |
| EarI | CTCTTC | 1 cut(s) | 597 |
| Ecl136II | GAGCTC | 2 cut(s) | 50, 172 |
| Eco130I | CCWWGG | 2 cut(s) | 533, 672 |
| Eco24I | GRGCYC | 3 cut(s) | 52, 174, 186 |
| Eco31I | GGTCTC | 1 cut(s) | 1134 |
| Eco47I | GGWCC | 1 cut(s) | 190 |
| Eco53kI | GAGCTC | 2 cut(s) | 50, 172 |
| EcoICRI | GAGCTC | 2 cut(s) | 50, 172 |
| EcoRII | CCWGG | 2 cut(s) | 338, 1036 |
| EcoT14I | CCWWGG | 2 cut(s) | 533, 672 |
| EcoT22I | ATGCAT | 2 cut(s) | 691, 837 |
| EcoT38I | GRGCYC | 3 cut(s) | 52, 174, 186 |
| ErhI | CCWWGG | 2 cut(s) | 533, 672 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 45, 77 |
| FaqI | GGGAC | 2 cut(s) | 619, 763 |
| Fnu4HI | GCNGC | 2 cut(s) | 468, 1083 |
| FokI | GGATG | 3 cut(s) | 218, 656, 1016 |
| FriOI | GRGCYC | 3 cut(s) | 52, 174, 186 |
| Fsp4HI | GCNGC | 2 cut(s) | 468, 1083 |
| FspAI | RTGCGCAY | 1 cut(s) | 230 |
| FspBI | CTAG | 3 cut(s) | 30, 119, 525 |
| FspI | TGCGCA | 1 cut(s) | 230 |
| GlaI | GCGC | 1 cut(s) | 230 |
| GluI | GCNGC | 2 cut(s) | 468, 1083 |
| GsuI | CTGGAG | 1 cut(s) | 786 |
| HaeIII | GGCC | 4 cut(s) | 150, 179, 671, 677 |
| HapII | CCGG | 2 cut(s) | 180, 401 |
| HhaI | GCGC | 1 cut(s) | 231 |
| Hin6I | GCGC | 1 cut(s) | 229 |
| HinP1I | GCGC | 1 cut(s) | 229 |
| HindIII | AAGCTT | 1 cut(s) | 578 |
| HinfI | GANTC | 3 cut(s) | 136, 572, 1089 |
| HpaII | CCGG | 2 cut(s) | 180, 401 |
| HphI | GGTGA | 1 cut(s) | 467 |
| Hpy166II | GTNNAC | 4 cut(s) | 193, 241, 479, 1144 |
| Hpy188I | TCNGA | 4 cut(s) | 47, 169, 587, 1060 |
| Hpy188III | TCNNGA | 3 cut(s) | 278, 388, 660 |
| Hpy8I | GTNNAC | 4 cut(s) | 193, 241, 479, 1144 |
| HpyAV | CCTTC | 2 cut(s) | 207, 582 |
| HpyCH4III | ACNGT | 3 cut(s) | 310, 452, 712 |
| HpyCH4IV | ACGT | 1 cut(s) | 132 |
| HpyCH4V | TGCA | 7 cut(s) | 110, 257, 689, 708, 745, 812, 835 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 228, 566 |
| HpyF3I | CTNAG | 3 cut(s) | 57, 896, 999 |
| HpySE526I | ACGT | 1 cut(s) | 132 |
| HspAI | GCGC | 1 cut(s) | 229 |
| Kzo9I | GATC | 5 cut(s) | 280, 397, 460, 656, 1071 |
| LmnI | GCTCC | 3 cut(s) | 47, 431, 521 |
| Lsp1109I | GCAGC | 2 cut(s) | 454, 1069 |
| LweI | GCATC | 3 cut(s) | 132, 240, 799 |
| MaeI | CTAG | 3 cut(s) | 30, 119, 525 |
| MaeII | ACGT | 1 cut(s) | 132 |
| MaeIII | GTNAC | 4 cut(s) | 96, 406, 542, 859 |
| MalI | GATC | 5 cut(s) | 282, 399, 462, 658, 1073 |
| MboI | GATC | 5 cut(s) | 280, 397, 460, 656, 1071 |
| MfeI | CAATTG | 1 cut(s) | 990 |
| MflI | RGATCY | 1 cut(s) | 1071 |
| MhlI | GDGCHC | 3 cut(s) | 52, 174, 186 |
| MlsI | TGGCCA | 1 cut(s) | 671 |
| MluCI | AATT | 5 cut(s) | 247, 515, 760, 813, 990 |
| MluNI | TGGCCA | 1 cut(s) | 671 |
| MlyI | GAGTC | 1 cut(s) | 566 |
| MmeI | TCCRAC | 1 cut(s) | 628 |
| Mox20I | TGGCCA | 1 cut(s) | 671 |
| Mph1103I | ATGCAT | 2 cut(s) | 691, 837 |
| MscI | TGGCCA | 1 cut(s) | 671 |
| MseI | TTAA | 3 cut(s) | 365, 552, 822 |
| MslI | CAYNNNNRTG | 4 cut(s) | 103, 267, 282, 944 |
| Msp20I | TGGCCA | 1 cut(s) | 671 |
| MspA1I | CMGCKG | 1 cut(s) | 467 |
| MspCI | CTTAAG | 1 cut(s) | 821 |
| MspI | CCGG | 2 cut(s) | 180, 401 |
| MspR9I | CCNGG | 3 cut(s) | 181, 340, 1038 |
| MunI | CAATTG | 1 cut(s) | 990 |
| Mva1269I | GAATGC | 2 cut(s) | 507, 689 |
| MvaI | CCWGG | 2 cut(s) | 340, 1038 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 228, 566 |
| NciI | CCSGG | 1 cut(s) | 181 |
| NdeII | GATC | 5 cut(s) | 280, 397, 460, 656, 1071 |
| NlaIV | GGNNCC | 2 cut(s) | 68, 531 |
| NmuCI | GTSAC | 3 cut(s) | 96, 406, 859 |
| NsbI | TGCGCA | 1 cut(s) | 230 |
| NsiI | ATGCAT | 2 cut(s) | 691, 837 |
| NspI | RCATGY | 3 cut(s) | 477, 731, 787 |
| PagI | TCATGA | 2 cut(s) | 277, 387 |
| PciI | ACATGT | 3 cut(s) | 473, 727, 783 |
| PctI | GAATGC | 2 cut(s) | 507, 689 |
| PfeI | GAWTC | 2 cut(s) | 136, 1089 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 763 |
| PkrI | GCNGC | 2 cut(s) | 469, 1084 |
| PleI | GAGTC | 1 cut(s) | 566 |
| PpsI | GAGTC | 1 cut(s) | 566 |
| PscI | ACATGT | 3 cut(s) | 473, 727, 783 |
| Psp124BI | GAGCTC | 2 cut(s) | 52, 174 |
| Psp6I | CCWGG | 2 cut(s) | 338, 1036 |
| PspGI | CCWGG | 2 cut(s) | 338, 1036 |
| PspN4I | GGNNCC | 2 cut(s) | 68, 531 |
| PspPI | GGNCC | 1 cut(s) | 190 |
| PsuI | RGATCY | 1 cut(s) | 1071 |
| PvuII | CAGCTG | 1 cut(s) | 467 |
| RsaI | GTAC | 1 cut(s) | 478 |
| RsaNI | GTAC | 1 cut(s) | 477 |
| RseI | CAYNNNNRTG | 4 cut(s) | 103, 267, 282, 944 |
| SacI | GAGCTC | 2 cut(s) | 52, 174 |
| SaqAI | TTAA | 3 cut(s) | 365, 552, 822 |
| SatI | GCNGC | 2 cut(s) | 468, 1083 |
| Sau3AI | GATC | 5 cut(s) | 280, 397, 460, 656, 1071 |
| Sau96I | GGNCC | 1 cut(s) | 190 |
| SchI | GAGTC | 1 cut(s) | 566 |
| ScrFI | CCNGG | 3 cut(s) | 181, 340, 1038 |
| SduI | GDGCHC | 3 cut(s) | 52, 174, 186 |
| SfaNI | GCATC | 3 cut(s) | 132, 240, 799 |
| SinI | GGWCC | 1 cut(s) | 190 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 103, 267, 282, 944 |
| SmlI | CTYRAG | 3 cut(s) | 51, 185, 821 |
| SmoI | CTYRAG | 3 cut(s) | 51, 185, 821 |
| Sse9I | AATT | 5 cut(s) | 247, 515, 760, 813, 990 |
| SspI | AATATT | 1 cut(s) | 867 |
| SspMI | CTAG | 3 cut(s) | 30, 119, 525 |
| SstI | GAGCTC | 2 cut(s) | 52, 174 |
| StyD4I | CCNGG | 3 cut(s) | 179, 338, 1036 |
| StyI | CCWWGG | 2 cut(s) | 533, 672 |
| TaaI | ACNGT | 3 cut(s) | 310, 452, 712 |
| TaiI | ACGT | 1 cut(s) | 135 |
| TaqI | TCGA | 2 cut(s) | 396, 1011 |
| TasI | AATT | 5 cut(s) | 247, 515, 760, 813, 990 |
| TatI | WGTACW | 1 cut(s) | 476 |
| TfiI | GAWTC | 2 cut(s) | 136, 1089 |
| Tru1I | TTAA | 3 cut(s) | 365, 552, 822 |
| Tru9I | TTAA | 3 cut(s) | 365, 552, 822 |
| TscAI | CASTG | 1 cut(s) | 715 |
| TseFI | GTSAC | 3 cut(s) | 96, 406, 859 |
| TseI | GCWGC | 2 cut(s) | 467, 1082 |
| Tsp45I | GTSAC | 3 cut(s) | 96, 406, 859 |
| TspDTI | ATGAA | 5 cut(s) | 30, 73, 263, 570, 864 |
| TspRI | CASTG | 1 cut(s) | 715 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 763 |
| Vha464I | CTTAAG | 1 cut(s) | 821 |
| VpaK11BI | GGWCC | 1 cut(s) | 190 |
| XceI | RCATGY | 3 cut(s) | 477, 731, 787 |
| XspI | CTAG | 3 cut(s) | 30, 119, 525 |
| Zsp2I | ATGCAT | 2 cut(s) | 691, 837 |