Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 133 |
| AciI | CCGC | 3 cut(s) | 160, 229, 443 |
| AclWI | GGATC | 1 cut(s) | 348 |
| AcoI | YGGCCR | 2 cut(s) | 157, 633 |
| AcuI | CTGAAG | 1 cut(s) | 334 |
| AfaI | GTAC | 1 cut(s) | 678 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 22, 72, 337, 694 |
| AflII | CTTAAG | 1 cut(s) | 335 |
| AflIII | ACRYGT | 2 cut(s) | 691, 744 |
| AgsI | TTSAA | 4 cut(s) | 56, 125, 517, 784 |
| AjnI | CCWGG | 1 cut(s) | 489 |
| AluBI | AGCT | 7 cut(s) | 49, 59, 90, 181, 328, 1009, 1050 |
| AluI | AGCT | 7 cut(s) | 49, 59, 90, 181, 328, 1009, 1050 |
| Alw21I | GWGCWC | 1 cut(s) | 183 |
| Alw26I | GTCTC | 1 cut(s) | 926 |
| AlwI | GGATC | 1 cut(s) | 348 |
| AoxI | GGCC | 3 cut(s) | 157, 633, 639 |
| ApeKI | GCWGC | 2 cut(s) | 1022, 1057 |
| AspS9I | GGNCC | 1 cut(s) | 247 |
| AsuHPI | GGTGA | 5 cut(s) | 41, 194, 335, 559, 889 |
| AvaII | GGWCC | 1 cut(s) | 247 |
| BalI | TGGCCA | 1 cut(s) | 635 |
| BanII | GRGCYC | 2 cut(s) | 183, 1006 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 291, 323 |
| BbsI | GAAGAC | 1 cut(s) | 819 |
| Bbv12I | GWGCWC | 1 cut(s) | 183 |
| BbvI | GCAGC | 2 cut(s) | 1034, 1069 |
| BccI | CCATC | 1 cut(s) | 1038 |
| BceAI | ACGGC | 1 cut(s) | 471 |
| BciT130I | CCWGG | 1 cut(s) | 491 |
| BcoDI | GTCTC | 1 cut(s) | 926 |
| BfaI | CTAG | 2 cut(s) | 128, 531 |
| BfmI | CTRYAG | 1 cut(s) | 481 |
| BfrI | CTTAAG | 1 cut(s) | 335 |
| BisI | GCNGC | 4 cut(s) | 160, 444, 1023, 1058 |
| BlpI | GCTNAGC | 1 cut(s) | 1005 |
| BlsI | GCNGC | 4 cut(s) | 161, 445, 1024, 1059 |
| Bme1390I | CCNGG | 1 cut(s) | 491 |
| Bme18I | GGWCC | 1 cut(s) | 247 |
| BmgT120I | GGNCC | 1 cut(s) | 247 |
| BmiI | GGNNCC | 3 cut(s) | 77, 425, 495 |
| BmrFI | CCNGG | 1 cut(s) | 491 |
| BmrI | ACTGGG | 1 cut(s) | 213 |
| BmsI | GCATC | 6 cut(s) | 267, 511, 640, 760, 1009, 1044 |
| BmuI | ACTGGG | 1 cut(s) | 213 |
| BpiI | GAAGAC | 1 cut(s) | 819 |
| BpmI | CTGGAG | 2 cut(s) | 756, 823 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1005 |
| BpuEI | CTTGAG | 2 cut(s) | 45, 851 |
| BsaBI | GATNNNNATC | 1 cut(s) | 595 |
| BsaJI | CCNNGG | 4 cut(s) | 401, 489, 525, 636 |
| BsaWI | WCCGGW | 1 cut(s) | 974 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 669, 699 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 22, 72, 337, 694 |
| Bse1I | ACTGG | 4 cut(s) | 219, 291, 806, 1114 |
| Bse8I | GATNNNNATC | 1 cut(s) | 595 |
| BseBI | CCWGG | 1 cut(s) | 491 |
| BseDI | CCNNGG | 4 cut(s) | 401, 489, 525, 636 |
| BseGI | GGATG | 3 cut(s) | 295, 471, 1030 |
| BseJI | GATNNNNATC | 1 cut(s) | 595 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 22, 72, 337, 694 |
| BseMII | CTCAG | 3 cut(s) | 168, 338, 1019 |
| BseNI | ACTGG | 4 cut(s) | 219, 291, 806, 1114 |
| BseRI | GAGGAG | 1 cut(s) | 320 |
| BseXI | GCAGC | 2 cut(s) | 1034, 1069 |
| BseYI | CCCAGC | 1 cut(s) | 45 |
| BsgI | GTGCAG | 1 cut(s) | 655 |
| BshFI | GGCC | 3 cut(s) | 159, 635, 641 |
| BsiHKAI | GWGCWC | 1 cut(s) | 183 |
| BsiSI | CCGG | 3 cut(s) | 255, 764, 975 |
| BslFI | GGGAC | 2 cut(s) | 491, 755 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 22, 72, 337, 694 |
| BsmAI | GTCTC | 1 cut(s) | 926 |
| BsmFI | GGGAC | 2 cut(s) | 491, 755 |
| BsmI | GAATGC | 2 cut(s) | 37, 653 |
| BsnI | GGCC | 3 cut(s) | 159, 635, 641 |
| Bsp1286I | GDGCHC | 2 cut(s) | 183, 1006 |
| Bsp143I | GATC | 4 cut(s) | 340, 682, 703, 916 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1005 |
| Bsp19I | CCATGG | 2 cut(s) | 401, 525 |
| BspACI | CCGC | 3 cut(s) | 160, 229, 443 |
| BspANI | GGCC | 3 cut(s) | 159, 635, 641 |
| BspCNI | CTCAG | 3 cut(s) | 169, 337, 1018 |
| BspLI | GGNNCC | 3 cut(s) | 77, 425, 495 |
| BspPI | GGATC | 1 cut(s) | 348 |
| BspQI | GCTCTTC | 1 cut(s) | 27 |
| BspTI | CTTAAG | 1 cut(s) | 335 |
| BsrI | ACTGG | 4 cut(s) | 219, 291, 806, 1114 |
| BssECI | CCNNGG | 4 cut(s) | 401, 489, 525, 636 |
| BssMI | GATC | 4 cut(s) | 340, 682, 703, 916 |
| BssNAI | GTATAC | 1 cut(s) | 134 |
| BssT1I | CCWWGG | 3 cut(s) | 401, 525, 636 |
| Bst1107I | GTATAC | 1 cut(s) | 134 |
| Bst2UI | CCWGG | 1 cut(s) | 491 |
| Bst4CI | ACNGT | 4 cut(s) | 676, 942, 1046, 1072 |
| Bst6I | CTCTTC | 1 cut(s) | 27 |
| BstAFI | CTTAAG | 1 cut(s) | 335 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1087, 1105 |
| BstC8I | GCNNGC | 3 cut(s) | 1027, 1097, 1101 |
| BstDEI | CTNAG | 3 cut(s) | 177, 324, 1005 |
| BstDSI | CCRYGG | 2 cut(s) | 401, 525 |
| BstEII | GGTNACC | 1 cut(s) | 547 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 335 |
| BstF5I | GGATG | 3 cut(s) | 295, 471, 1030 |
| BstKTI | GATC | 4 cut(s) | 343, 685, 706, 919 |
| BstMAI | GTCTC | 1 cut(s) | 926 |
| BstMBI | GATC | 4 cut(s) | 340, 682, 703, 916 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1087, 1096, 1105 |
| BstNI | CCWGG | 1 cut(s) | 491 |
| BstNSI | RCATGY | 3 cut(s) | 695, 748, 1029 |
| BstPI | GGTNACC | 1 cut(s) | 547 |
| BstSCI | CCNGG | 1 cut(s) | 489 |
| BstSFI | CTRYAG | 1 cut(s) | 481 |
| BstV1I | GCAGC | 2 cut(s) | 1034, 1069 |
| BstV2I | GAAGAC | 1 cut(s) | 819 |
| BstZ17I | GTATAC | 1 cut(s) | 134 |
| BsuRI | GGCC | 3 cut(s) | 159, 635, 641 |
| BtgI | CCRYGG | 2 cut(s) | 401, 525 |
| BtgZI | GCGATG | 1 cut(s) | 850 |
| BtsCI | GGATG | 3 cut(s) | 295, 471, 1030 |
| BtsI | GCAGTG | 1 cut(s) | 1103 |
| BtsIMutI | CAGTG | 2 cut(s) | 226, 1103 |
| Cac8I | GCNNGC | 3 cut(s) | 1027, 1097, 1101 |
| Cfr13I | GGNCC | 1 cut(s) | 247 |
| CseI | GACGC | 1 cut(s) | 326 |
| Csp6I | GTAC | 1 cut(s) | 677 |
| CviQI | GTAC | 1 cut(s) | 677 |
| DdeI | CTNAG | 3 cut(s) | 177, 324, 1005 |
| DpnI | GATC | 4 cut(s) | 342, 684, 705, 918 |
| DpnII | GATC | 4 cut(s) | 340, 682, 703, 916 |
| EaeI | YGGCCR | 2 cut(s) | 157, 633 |
| Eam1104I | CTCTTC | 1 cut(s) | 27 |
| EarI | CTCTTC | 1 cut(s) | 27 |
| Ecl136II | GAGCTC | 1 cut(s) | 181 |
| Eco130I | CCWWGG | 3 cut(s) | 401, 525, 636 |
| Eco24I | GRGCYC | 2 cut(s) | 183, 1006 |
| Eco32I | GATATC | 1 cut(s) | 142 |
| Eco47I | GGWCC | 1 cut(s) | 247 |
| Eco53kI | GAGCTC | 1 cut(s) | 181 |
| Eco57I | CTGAAG | 1 cut(s) | 334 |
| Eco91I | GGTNACC | 1 cut(s) | 547 |
| EcoICRI | GAGCTC | 1 cut(s) | 181 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 335 |
| EcoO65I | GGTNACC | 1 cut(s) | 547 |
| EcoRII | CCWGG | 1 cut(s) | 489 |
| EcoRV | GATATC | 1 cut(s) | 142 |
| EcoT14I | CCWWGG | 3 cut(s) | 401, 525, 636 |
| EcoT22I | ATGCAT | 1 cut(s) | 655 |
| EcoT38I | GRGCYC | 2 cut(s) | 183, 1006 |
| ErhI | CCWWGG | 3 cut(s) | 401, 525, 636 |
| FaqI | GGGAC | 2 cut(s) | 491, 755 |
| FauNDI | CATATG | 2 cut(s) | 71, 460 |
| FblI | GTMKAC | 1 cut(s) | 133 |
| Fnu4HI | GCNGC | 4 cut(s) | 160, 444, 1023, 1058 |
| FokI | GGATG | 3 cut(s) | 302, 478, 1017 |
| FriOI | GRGCYC | 2 cut(s) | 183, 1006 |
| Fsp4HI | GCNGC | 4 cut(s) | 160, 444, 1023, 1058 |
| FspBI | CTAG | 2 cut(s) | 128, 531 |
| GluI | GCNGC | 4 cut(s) | 160, 444, 1023, 1058 |
| GsaI | CCCAGC | 1 cut(s) | 49 |
| GsuI | CTGGAG | 2 cut(s) | 756, 823 |
| HaeIII | GGCC | 3 cut(s) | 159, 635, 641 |
| HapII | CCGG | 3 cut(s) | 255, 764, 975 |
| HgaI | GACGC | 1 cut(s) | 326 |
| HincII | GTYRAC | 2 cut(s) | 279, 1075 |
| HindII | GTYRAC | 2 cut(s) | 279, 1075 |
| HinfI | GANTC | 2 cut(s) | 815, 923 |
| HpaI | GTTAAC | 1 cut(s) | 1075 |
| HpaII | CCGG | 3 cut(s) | 255, 764, 975 |
| HphI | GGTGA | 5 cut(s) | 41, 194, 335, 559, 889 |
| Hpy166II | GTNNAC | 5 cut(s) | 134, 226, 250, 279, 1075 |
| Hpy188I | TCNGA | 2 cut(s) | 178, 1018 |
| Hpy188III | TCNNGA | 1 cut(s) | 920 |
| Hpy8I | GTNNAC | 5 cut(s) | 134, 226, 250, 279, 1075 |
| HpyAV | CCTTC | 2 cut(s) | 293, 358 |
| HpyCH4III | ACNGT | 4 cut(s) | 676, 942, 1046, 1072 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1087, 1096, 1105 |
| HpyF3I | CTNAG | 3 cut(s) | 177, 324, 1005 |
| KspAI | GTTAAC | 1 cut(s) | 1075 |
| Kzo9I | GATC | 4 cut(s) | 340, 682, 703, 916 |
| LguI | GCTCTTC | 1 cut(s) | 27 |
| LmnI | GCTCC | 2 cut(s) | 333, 493 |
| Lsp1109I | GCAGC | 2 cut(s) | 1034, 1069 |
| LweI | GCATC | 6 cut(s) | 267, 511, 640, 760, 1009, 1044 |
| MaeI | CTAG | 2 cut(s) | 128, 531 |
| MaeIII | GTNAC | 3 cut(s) | 391, 547, 558 |
| MalI | GATC | 4 cut(s) | 342, 684, 705, 918 |
| MboI | GATC | 4 cut(s) | 340, 682, 703, 916 |
| MboII | GAAGA | 5 cut(s) | 44, 44, 308, 824, 877 |
| MfeI | CAATTG | 2 cut(s) | 120, 927 |
| MhlI | GDGCHC | 2 cut(s) | 183, 1006 |
| MlsI | TGGCCA | 1 cut(s) | 635 |
| MluCI | AATT | 5 cut(s) | 120, 198, 663, 774, 927 |
| MluNI | TGGCCA | 1 cut(s) | 635 |
| MlyI | GAGTC | 1 cut(s) | 809 |
| MmeI | TCCRAC | 1 cut(s) | 1058 |
| MnlI | CCTC | 9 cut(s) | 119, 242, 286, 341, 580, 731, 745, 771, 838 |
| Mox20I | TGGCCA | 1 cut(s) | 635 |
| Mph1103I | ATGCAT | 1 cut(s) | 655 |
| MscI | TGGCCA | 1 cut(s) | 635 |
| MseI | TTAA | 5 cut(s) | 336, 450, 564, 593, 1074 |
| MslI | CAYNNNNRTG | 2 cut(s) | 890, 956 |
| Msp20I | TGGCCA | 1 cut(s) | 635 |
| MspCI | CTTAAG | 1 cut(s) | 335 |
| MspI | CCGG | 3 cut(s) | 255, 764, 975 |
| MspR9I | CCNGG | 1 cut(s) | 491 |
| MunI | CAATTG | 2 cut(s) | 120, 927 |
| Mva1269I | GAATGC | 2 cut(s) | 37, 653 |
| MvaI | CCWGG | 1 cut(s) | 491 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1087, 1096, 1105 |
| NcoI | CCATGG | 2 cut(s) | 401, 525 |
| NdeI | CATATG | 2 cut(s) | 71, 460 |
| NdeII | GATC | 4 cut(s) | 340, 682, 703, 916 |
| NlaIV | GGNNCC | 3 cut(s) | 77, 425, 495 |
| NmuCI | GTSAC | 2 cut(s) | 391, 547 |
| NsiI | ATGCAT | 1 cut(s) | 655 |
| NspI | RCATGY | 3 cut(s) | 695, 748, 1029 |
| PaeI | GCATGC | 1 cut(s) | 1029 |
| PciI | ACATGT | 2 cut(s) | 691, 744 |
| PciSI | GCTCTTC | 1 cut(s) | 27 |
| PctI | GAATGC | 2 cut(s) | 37, 653 |
| PfeI | GAWTC | 1 cut(s) | 923 |
| PkrI | GCNGC | 4 cut(s) | 161, 445, 1024, 1059 |
| PleI | GAGTC | 1 cut(s) | 809 |
| PpsI | GAGTC | 1 cut(s) | 809 |
| PscI | ACATGT | 2 cut(s) | 691, 744 |
| Psp124BI | GAGCTC | 1 cut(s) | 183 |
| Psp6I | CCWGG | 1 cut(s) | 489 |
| PspEI | GGTNACC | 1 cut(s) | 547 |
| PspFI | CCCAGC | 1 cut(s) | 45 |
| PspGI | CCWGG | 1 cut(s) | 489 |
| PspN4I | GGNNCC | 3 cut(s) | 77, 425, 495 |
| PspPI | GGNCC | 1 cut(s) | 247 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 117, 149 |
| RsaI | GTAC | 1 cut(s) | 678 |
| RsaNI | GTAC | 1 cut(s) | 677 |
| RseI | CAYNNNNRTG | 2 cut(s) | 890, 956 |
| SacI | GAGCTC | 1 cut(s) | 183 |
| SapI | GCTCTTC | 1 cut(s) | 27 |
| SaqAI | TTAA | 5 cut(s) | 336, 450, 564, 593, 1074 |
| SatI | GCNGC | 4 cut(s) | 160, 444, 1023, 1058 |
| Sau3AI | GATC | 4 cut(s) | 340, 682, 703, 916 |
| Sau96I | GGNCC | 1 cut(s) | 247 |
| SchI | GAGTC | 1 cut(s) | 809 |
| ScrFI | CCNGG | 1 cut(s) | 491 |
| SduI | GDGCHC | 2 cut(s) | 183, 1006 |
| SfaNI | GCATC | 6 cut(s) | 267, 511, 640, 760, 1009, 1044 |
| SfcI | CTRYAG | 1 cut(s) | 481 |
| SinI | GGWCC | 1 cut(s) | 247 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 890, 956 |
| SmlI | CTYRAG | 3 cut(s) | 60, 335, 830 |
| SmoI | CTYRAG | 3 cut(s) | 60, 335, 830 |
| SphI | GCATGC | 1 cut(s) | 1029 |
| Sse9I | AATT | 5 cut(s) | 120, 198, 663, 774, 927 |
| SsiI | CCGC | 3 cut(s) | 160, 229, 443 |
| SspMI | CTAG | 2 cut(s) | 128, 531 |
| SstI | GAGCTC | 1 cut(s) | 183 |
| StyD4I | CCNGG | 1 cut(s) | 489 |
| StyI | CCWWGG | 3 cut(s) | 401, 525, 636 |
| TaaI | ACNGT | 4 cut(s) | 676, 942, 1046, 1072 |
| TaqI | TCGA | 1 cut(s) | 585 |
| TasI | AATT | 5 cut(s) | 120, 198, 663, 774, 927 |
| TauI | GCSGC | 2 cut(s) | 162, 446 |
| TfiI | GAWTC | 1 cut(s) | 923 |
| Tru1I | TTAA | 5 cut(s) | 336, 450, 564, 593, 1074 |
| Tru9I | TTAA | 5 cut(s) | 336, 450, 564, 593, 1074 |
| TscAI | CASTG | 2 cut(s) | 226, 1110 |
| TseFI | GTSAC | 2 cut(s) | 391, 547 |
| TseI | GCWGC | 2 cut(s) | 1022, 1057 |
| Tsp45I | GTSAC | 2 cut(s) | 391, 547 |
| TspDTI | ATGAA | 6 cut(s) | 82, 428, 825, 908, 1005, 1098 |
| TspRI | CASTG | 2 cut(s) | 226, 1110 |
| Vha464I | CTTAAG | 1 cut(s) | 335 |
| VpaK11BI | GGWCC | 1 cut(s) | 247 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 335 |
| XceI | RCATGY | 3 cut(s) | 695, 748, 1029 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 23 |
| XmiI | GTMKAC | 1 cut(s) | 133 |
| XspI | CTAG | 2 cut(s) | 128, 531 |
| Zsp2I | ATGCAT | 1 cut(s) | 655 |