Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 765 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AciI | CCGC | 1 cut(s) | 283 |
| AclWI | GGATC | 1 cut(s) | 399 |
| AcuI | CTGAAG | 2 cut(s) | 124, 407 |
| AfaI | GTAC | 2 cut(s) | 673, 729 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 126, 388, 745, 1106 |
| AflII | CTTAAG | 2 cut(s) | 400, 881 |
| AflIII | ACRYGT | 2 cut(s) | 742, 795 |
| AgsI | TTSAA | 5 cut(s) | 179, 568, 719, 778, 983 |
| AleI | CACNNNNGTG | 1 cut(s) | 605 |
| AluBI | AGCT | 8 cut(s) | 54, 103, 144, 235, 331, 379, 991, 1060 |
| AluI | AGCT | 8 cut(s) | 54, 103, 144, 235, 331, 379, 991, 1060 |
| Alw21I | GWGCWC | 3 cut(s) | 237, 306, 470 |
| Alw26I | GTCTC | 1 cut(s) | 1122 |
| Alw44I | GTGCAC | 1 cut(s) | 302 |
| AlwI | GGATC | 1 cut(s) | 399 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 680 |
| AoxI | GGCC | 2 cut(s) | 690, 1014 |
| ApaLI | GTGCAC | 1 cut(s) | 302 |
| ApeKI | GCWGC | 1 cut(s) | 988 |
| AspS9I | GGNCC | 1 cut(s) | 1015 |
| AsuHPI | GGTGA | 3 cut(s) | 592, 803, 940 |
| AsuNHI | GCTAGC | 1 cut(s) | 581 |
| BaeGI | GKGCMC | 1 cut(s) | 306 |
| BanII | GRGCYC | 1 cut(s) | 237 |
| Bbv12I | GWGCWC | 3 cut(s) | 237, 306, 470 |
| BbvI | GCAGC | 1 cut(s) | 1000 |
| BccI | CCATC | 3 cut(s) | 549, 943, 1006 |
| BclI | TGATCA | 2 cut(s) | 30, 609 |
| BcoDI | GTCTC | 1 cut(s) | 1122 |
| BfaI | CTAG | 3 cut(s) | 182, 582, 891 |
| BfrI | CTTAAG | 2 cut(s) | 400, 881 |
| BfuAI | ACCTGC | 1 cut(s) | 765 |
| BisI | GCNGC | 1 cut(s) | 989 |
| BlpI | GCTNAGC | 1 cut(s) | 1056 |
| BlsI | GCNGC | 1 cut(s) | 990 |
| BmgT120I | GGNCC | 1 cut(s) | 1015 |
| BmiI | GGNNCC | 3 cut(s) | 131, 476, 539 |
| BmsI | GCATC | 6 cut(s) | 285, 358, 562, 577, 691, 1060 |
| BmtI | GCTAGC | 1 cut(s) | 585 |
| BpmI | CTGGAG | 1 cut(s) | 807 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1056 |
| BpuEI | CTTGAG | 3 cut(s) | 99, 484, 818 |
| Bsa29I | ATCGAT | 1 cut(s) | 615 |
| BsaI | GGTCTC | 1 cut(s) | 1122 |
| BsaJI | CCNNGG | 2 cut(s) | 452, 576 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 720, 750 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 126, 388, 745, 1106 |
| Bse1I | ACTGG | 2 cut(s) | 790, 857 |
| Bse3DI | GCAATG | 1 cut(s) | 693 |
| BseCI | ATCGAT | 1 cut(s) | 615 |
| BseDI | CCNNGG | 2 cut(s) | 452, 576 |
| BseGI | GGATG | 2 cut(s) | 394, 522 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 126, 388, 745, 1106 |
| BseMI | GCAATG | 1 cut(s) | 693 |
| BseMII | CTCAG | 3 cut(s) | 15, 222, 323 |
| BseNI | ACTGG | 2 cut(s) | 790, 857 |
| BseSI | GKGCMC | 1 cut(s) | 306 |
| BseXI | GCAGC | 1 cut(s) | 1000 |
| BseYI | CCCAGC | 1 cut(s) | 375 |
| BsgI | GTGCAG | 1 cut(s) | 228 |
| BshFI | GGCC | 2 cut(s) | 692, 1016 |
| BshVI | ATCGAT | 1 cut(s) | 615 |
| BsiHKAI | GWGCWC | 3 cut(s) | 237, 306, 470 |
| BslFI | GGGAC | 1 cut(s) | 145 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 126, 388, 745, 1106 |
| BsmAI | GTCTC | 1 cut(s) | 1122 |
| BsmFI | GGGAC | 1 cut(s) | 145 |
| BsmI | GAATGC | 3 cut(s) | 91, 704, 725 |
| BsnI | GGCC | 2 cut(s) | 692, 1016 |
| Bso31I | GGTCTC | 1 cut(s) | 1122 |
| Bsp1286I | GDGCHC | 3 cut(s) | 237, 306, 470 |
| Bsp143I | GATC | 5 cut(s) | 30, 391, 609, 733, 887 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1056 |
| Bsp19I | CCATGG | 2 cut(s) | 452, 576 |
| BspACI | CCGC | 1 cut(s) | 283 |
| BspANI | GGCC | 2 cut(s) | 692, 1016 |
| BspCNI | CTCAG | 3 cut(s) | 16, 223, 324 |
| BspDI | ATCGAT | 1 cut(s) | 615 |
| BspLI | GGNNCC | 3 cut(s) | 131, 476, 539 |
| BspMI | ACCTGC | 1 cut(s) | 765 |
| BspOI | GCTAGC | 1 cut(s) | 585 |
| BspPI | GGATC | 1 cut(s) | 399 |
| BspQI | GCTCTTC | 1 cut(s) | 1067 |
| BspTI | CTTAAG | 2 cut(s) | 400, 881 |
| BspTNI | GGTCTC | 1 cut(s) | 1122 |
| BsrDI | GCAATG | 1 cut(s) | 693 |
| BsrI | ACTGG | 2 cut(s) | 790, 857 |
| BssECI | CCNNGG | 2 cut(s) | 452, 576 |
| BssMI | GATC | 5 cut(s) | 30, 391, 609, 733, 887 |
| BssNAI | GTATAC | 1 cut(s) | 188 |
| BssT1I | CCWWGG | 2 cut(s) | 452, 576 |
| Bst1107I | GTATAC | 1 cut(s) | 188 |
| Bst4CI | ACNGT | 3 cut(s) | 682, 727, 826 |
| Bst6I | CTCTTC | 1 cut(s) | 1067 |
| BstAFI | CTTAAG | 2 cut(s) | 400, 881 |
| BstC8I | GCNNGC | 1 cut(s) | 583 |
| BstDEI | CTNAG | 5 cut(s) | 24, 231, 321, 332, 1056 |
| BstDSI | CCRYGG | 2 cut(s) | 452, 576 |
| BstEII | GGTNACC | 1 cut(s) | 598 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 386 |
| BstF5I | GGATG | 2 cut(s) | 394, 522 |
| BstKTI | GATC | 5 cut(s) | 33, 394, 612, 736, 890 |
| BstMAI | GTCTC | 1 cut(s) | 1122 |
| BstMBI | GATC | 5 cut(s) | 30, 391, 609, 733, 887 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 282, 587 |
| BstNSI | RCATGY | 3 cut(s) | 746, 799, 1006 |
| BstPI | GGTNACC | 1 cut(s) | 598 |
| BstSLI | GKGCMC | 1 cut(s) | 306 |
| BstV1I | GCAGC | 1 cut(s) | 1000 |
| BstZ17I | GTATAC | 1 cut(s) | 188 |
| Bsu15I | ATCGAT | 1 cut(s) | 615 |
| BsuRI | GGCC | 2 cut(s) | 692, 1016 |
| BsuTUI | ATCGAT | 1 cut(s) | 615 |
| BtgI | CCRYGG | 2 cut(s) | 452, 576 |
| BtgZI | GCGATG | 2 cut(s) | 600, 981 |
| BtsCI | GGATG | 2 cut(s) | 394, 522 |
| BtsIMutI | CAGTG | 4 cut(s) | 21, 723, 1065, 1087 |
| BveI | ACCTGC | 1 cut(s) | 765 |
| Cac8I | GCNNGC | 1 cut(s) | 583 |
| CaiI | CAGNNNCTG | 1 cut(s) | 680 |
| Cfr13I | GGNCC | 1 cut(s) | 1015 |
| ClaI | ATCGAT | 1 cut(s) | 615 |
| Csp6I | GTAC | 2 cut(s) | 672, 728 |
| CviQI | GTAC | 2 cut(s) | 672, 728 |
| DdeI | CTNAG | 5 cut(s) | 24, 231, 321, 332, 1056 |
| DpnI | GATC | 5 cut(s) | 32, 393, 611, 735, 889 |
| DpnII | GATC | 5 cut(s) | 30, 391, 609, 733, 887 |
| Eam1104I | CTCTTC | 1 cut(s) | 1067 |
| EarI | CTCTTC | 1 cut(s) | 1067 |
| Ecl136II | GAGCTC | 1 cut(s) | 235 |
| Eco130I | CCWWGG | 2 cut(s) | 452, 576 |
| Eco24I | GRGCYC | 1 cut(s) | 237 |
| Eco31I | GGTCTC | 1 cut(s) | 1122 |
| Eco32I | GATATC | 1 cut(s) | 196 |
| Eco53kI | GAGCTC | 1 cut(s) | 235 |
| Eco57I | CTGAAG | 2 cut(s) | 124, 407 |
| Eco91I | GGTNACC | 1 cut(s) | 598 |
| EcoICRI | GAGCTC | 1 cut(s) | 235 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 386 |
| EcoO65I | GGTNACC | 1 cut(s) | 598 |
| EcoRV | GATATC | 1 cut(s) | 196 |
| EcoT14I | CCWWGG | 2 cut(s) | 452, 576 |
| EcoT22I | ATGCAT | 1 cut(s) | 706 |
| EcoT38I | GRGCYC | 1 cut(s) | 237 |
| ErhI | CCWWGG | 2 cut(s) | 452, 576 |
| FaqI | GGGAC | 1 cut(s) | 145 |
| FbaI | TGATCA | 2 cut(s) | 30, 609 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 1 cut(s) | 989 |
| FokI | GGATG | 2 cut(s) | 381, 529 |
| FriOI | GRGCYC | 1 cut(s) | 237 |
| Fsp4HI | GCNGC | 1 cut(s) | 989 |
| FspBI | CTAG | 3 cut(s) | 182, 582, 891 |
| GluI | GCNGC | 1 cut(s) | 989 |
| GsaI | CCCAGC | 1 cut(s) | 379 |
| GsuI | CTGGAG | 1 cut(s) | 807 |
| HaeIII | GGCC | 2 cut(s) | 692, 1016 |
| HinfI | GANTC | 3 cut(s) | 223, 647, 974 |
| HphI | GGTGA | 3 cut(s) | 592, 803, 940 |
| Hpy166II | GTNNAC | 2 cut(s) | 188, 304 |
| Hpy188I | TCNGA | 3 cut(s) | 10, 232, 387 |
| Hpy188III | TCNNGA | 2 cut(s) | 127, 891 |
| Hpy8I | GTNNAC | 2 cut(s) | 188, 304 |
| HpyAV | CCTTC | 4 cut(s) | 344, 382, 392, 424 |
| HpyCH4III | ACNGT | 3 cut(s) | 682, 727, 826 |
| HpyCH4V | TGCA | 8 cut(s) | 173, 245, 304, 624, 704, 723, 760, 847 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 282, 587 |
| HpyF3I | CTNAG | 5 cut(s) | 24, 231, 321, 332, 1056 |
| Ksp22I | TGATCA | 2 cut(s) | 30, 609 |
| Kzo9I | GATC | 5 cut(s) | 30, 391, 609, 733, 887 |
| LguI | GCTCTTC | 1 cut(s) | 1067 |
| LmnI | GCTCC | 1 cut(s) | 384 |
| Lsp1109I | GCAGC | 1 cut(s) | 1000 |
| LweI | GCATC | 6 cut(s) | 285, 358, 562, 577, 691, 1060 |
| MaeI | CTAG | 3 cut(s) | 182, 582, 891 |
| MaeIII | GTNAC | 4 cut(s) | 598, 791, 1021, 1082 |
| MalI | GATC | 5 cut(s) | 32, 393, 611, 735, 889 |
| MboI | GATC | 5 cut(s) | 30, 391, 609, 733, 887 |
| MboII | GAAGA | 5 cut(s) | 130, 556, 875, 988, 1054 |
| MfeI | CAATTG | 1 cut(s) | 978 |
| MhlI | GDGCHC | 3 cut(s) | 237, 306, 470 |
| MluCI | AATT | 4 cut(s) | 174, 457, 900, 978 |
| MlyI | GAGTC | 2 cut(s) | 217, 656 |
| MmeI | TCCRAC | 2 cut(s) | 120, 643 |
| Mph1103I | ATGCAT | 1 cut(s) | 706 |
| MseI | TTAA | 5 cut(s) | 66, 401, 419, 882, 1112 |
| MslI | CAYNNNNRTG | 3 cut(s) | 605, 941, 1007 |
| MspA1I | CMGCKG | 1 cut(s) | 991 |
| MspCI | CTTAAG | 2 cut(s) | 400, 881 |
| MunI | CAATTG | 1 cut(s) | 978 |
| Mva1269I | GAATGC | 3 cut(s) | 91, 704, 725 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 282, 587 |
| NcoI | CCATGG | 2 cut(s) | 452, 576 |
| NdeII | GATC | 5 cut(s) | 30, 391, 609, 733, 887 |
| NheI | GCTAGC | 1 cut(s) | 581 |
| NlaIV | GGNNCC | 3 cut(s) | 131, 476, 539 |
| NmuCI | GTSAC | 4 cut(s) | 598, 791, 1021, 1082 |
| NsiI | ATGCAT | 1 cut(s) | 706 |
| NspI | RCATGY | 3 cut(s) | 746, 799, 1006 |
| OliI | CACNNNNGTG | 1 cut(s) | 605 |
| PciI | ACATGT | 2 cut(s) | 742, 795 |
| PciSI | GCTCTTC | 1 cut(s) | 1067 |
| PctI | GAATGC | 3 cut(s) | 91, 704, 725 |
| PfeI | GAWTC | 1 cut(s) | 974 |
| PkrI | GCNGC | 1 cut(s) | 990 |
| PleI | GAGTC | 2 cut(s) | 217, 655 |
| PpsI | GAGTC | 2 cut(s) | 217, 655 |
| PscI | ACATGT | 2 cut(s) | 742, 795 |
| Psp124BI | GAGCTC | 1 cut(s) | 237 |
| PspEI | GGTNACC | 1 cut(s) | 598 |
| PspFI | CCCAGC | 1 cut(s) | 375 |
| PspN4I | GGNNCC | 3 cut(s) | 131, 476, 539 |
| PspPI | GGNCC | 1 cut(s) | 1015 |
| PstNI | CAGNNNCTG | 1 cut(s) | 680 |
| PvuII | CAGCTG | 1 cut(s) | 991 |
| RsaI | GTAC | 2 cut(s) | 673, 729 |
| RsaNI | GTAC | 2 cut(s) | 672, 728 |
| RseI | CAYNNNNRTG | 3 cut(s) | 605, 941, 1007 |
| SacI | GAGCTC | 1 cut(s) | 237 |
| SapI | GCTCTTC | 1 cut(s) | 1067 |
| SaqAI | TTAA | 5 cut(s) | 66, 401, 419, 882, 1112 |
| SatI | GCNGC | 1 cut(s) | 989 |
| Sau3AI | GATC | 5 cut(s) | 30, 391, 609, 733, 887 |
| Sau96I | GGNCC | 1 cut(s) | 1015 |
| SchI | GAGTC | 2 cut(s) | 217, 656 |
| SduI | GDGCHC | 3 cut(s) | 237, 306, 470 |
| SfaNI | GCATC | 6 cut(s) | 285, 358, 562, 577, 691, 1060 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 605, 941, 1007 |
| SmlI | CTYRAG | 5 cut(s) | 114, 400, 463, 833, 881 |
| SmoI | CTYRAG | 5 cut(s) | 114, 400, 463, 833, 881 |
| Sse9I | AATT | 4 cut(s) | 174, 457, 900, 978 |
| SsiI | CCGC | 1 cut(s) | 283 |
| SspI | AATATT | 1 cut(s) | 297 |
| SspMI | CTAG | 3 cut(s) | 182, 582, 891 |
| SstI | GAGCTC | 1 cut(s) | 237 |
| StyI | CCWWGG | 2 cut(s) | 452, 576 |
| TaaI | ACNGT | 3 cut(s) | 682, 727, 826 |
| TaqI | TCGA | 4 cut(s) | 432, 615, 657, 999 |
| TasI | AATT | 4 cut(s) | 174, 457, 900, 978 |
| TatI | WGTACW | 1 cut(s) | 727 |
| TfiI | GAWTC | 1 cut(s) | 974 |
| Tru1I | TTAA | 5 cut(s) | 66, 401, 419, 882, 1112 |
| Tru9I | TTAA | 5 cut(s) | 66, 401, 419, 882, 1112 |
| TscAI | CASTG | 4 cut(s) | 28, 730, 1072, 1087 |
| TseFI | GTSAC | 4 cut(s) | 598, 791, 1021, 1082 |
| TseI | GCWGC | 1 cut(s) | 988 |
| Tsp45I | GTSAC | 4 cut(s) | 598, 791, 1021, 1082 |
| TspDTI | ATGAA | 3 cut(s) | 98, 136, 876 |
| TspRI | CASTG | 4 cut(s) | 28, 730, 1072, 1087 |
| Vha464I | CTTAAG | 2 cut(s) | 400, 881 |
| VneI | GTGCAC | 1 cut(s) | 302 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 386 |
| XbaI | TCTAGA | 1 cut(s) | 890 |
| XceI | RCATGY | 3 cut(s) | 746, 799, 1006 |
| XmiI | GTMKAC | 1 cut(s) | 187 |
| XspI | CTAG | 3 cut(s) | 182, 582, 891 |
| Zsp2I | ATGCAT | 1 cut(s) | 706 |