Rh2CG316800
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000692
Physical Location & Seq
Forward (+)
40385530 .. 40409864
24335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG316800.1

Sequence Viewer

Length: 795 bp
ATGGAAAGACTGAACCATAAGTGGGCTAGTGAAGAGCATTCCACCCAGCTTCTTCAAGCTCAAGCCCACATATGGAACCACATTTTCAGCTTCATAAACTCCATGACCCTCAAATCTGCAATTGAACTAGGTATACCAGATATCATCAAAAAACATGGCCGCCCCATAACTCTTTCTGAGCTCACATCTGCATTACCAATTCACCCAACAAAAATCCCCAGTGTTTACCGCCTCATGCGTATATTGGTCCACTCCGGCATCTTTGCAAAAAAAAAGCTGACTAAACTGGATGAGGAAGAAGGTTATATACTTACTGACGCTTCTCAGCTCCTCCTTAAGGATCACCCCTTCAGTATAACTCCATTTGTAATGGCTATGCTCAACCCTATCGTCACAAAACCATGGCATTATATGAACACTTGGTTCCAAAATAATGACCCTGCGGCATTTAACACGGCATATGGGATGACATTTTGGGACTACAGCACCCAGGAGCCAAGTCTTGCCAACTTTTTCAATGATGCCATGGCTAGTGATACTCGTTTGGTGACCAGCGTGTTACTTAAAGAGTGCAAGGGGGTATTCGAGGGATTAAAATCATTAGTTGATGTTGGGGGTGGTACAGGAACAATGGCCAAGGCCATTGTTGATGCATTCCCAGAAATTGACTGCACCGTACTTGATCTCCCACATGTGGTGGCTGATCTGCAAGGAAGTAAGAACCTGAAATATGCTGGAGGGGACATGTTTGAGGCAGTTCCTCCGGCAGATGCAATTATTTTGAAGTATGCATAA

Protein Analysis

264

Amino Acids

29.44

Weight (kDa)

6.04

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 25 - 114 9.5e-22 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 135 - 262 6.4e-32 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26800 FvH4_6g26810
malus_domestica MD01G1048000.v1.1 MD01G1048300.v1.1 MD01G1048400.v1.1 MD01G1051800.v1.1 MD01G1051900.v1.1 MD01G1149300.v1.1
prunus_persica Prupe.3G118900_v2.0.a1 Prupe.3G119100_v2.0.a1 Prupe.3G119700_v2.0.a1 Prupe.3G119800_v2.0.a1 Prupe.3G120200_v2.0.a1 Prupe.3G120400_v2.0.a1 Prupe.3G127600_v2.0.a1 Prupe.3G129000_v2.0.a1 Prupe.3G129200_v2.0.a1 Prupe.3G129400_v2.0.a1 Prupe.3G129600_v2.0.a1 Prupe.3G129800_v2.0.a1 Prupe.4G252900_v2.0.a1 Prupe.4G253200_v2.0.a1 Prupe.4G253300_v2.0.a1
pyrus_communis pycom01g07360 pycom01g07640 pycom01g07670 pycom01g07720 pycom01g07740 pycom01g07750
rosa_chinensis RchiOBHm_Chr2g0119291 RchiOBHm_Chr2g0123081 RchiOBHm_Chr2g0123091 RchiOBHm_Chr2g0128091 RchiOBHm_Chr2g0128121 RchiOBHm_Chr2g0128161 RchiOBHm_Chr4g0436611
rosa_laevigata RLG00000006507 RLG00000006508 RLG00000018994 RLG00000018995
rosa_multiflora Rmu_co8289337.1_g000001 Rmu_sc0000837.1_g000001 Rmu_sc0003130.1_g000011 Rmu_sc0027975.1_g000001
rosa_roxburghii Rroxscaffold_2G00115570 Rroxscaffold_2G00115600 Rroxscaffold_2G00116080 Rroxscaffold_2G00116090 Rroxscaffold_5G00377660
rosa_rugosa Rorug02G0278000 Rorug02G0278000 Rorug02G0278100 Rorug02G0558700 Rorug04G0294700
rosa_samantha Rh2AG330000 Rh2AG330300 Rh2AG330500 Rh2AG330600 Rh2AG330900 Rh2AG331100 Rh2BG310500 Rh2BG339200 Rh2BG339300 Rh2CG316800 Rh2DG304600 Rh2DG356000 Rh2DG356200 Rh4AG349100 Rh4BG357800 Rh4CG372300 Rh4DG352000 Rh5BG070000
rosa_wichuraiana Rw2G026730 Rw2G026760 Rw4G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 133
AciI CCGC 3 cut(s) 160, 229, 443
AclWI GGATC 1 cut(s) 348
AcoI YGGCCR 2 cut(s) 157, 633
AcuI CTGAAG 1 cut(s) 334
AfaI GTAC 2 cut(s) 622, 678
AfiI CCNNNNNNNGG 4 cut(s) 22, 72, 337, 694
AflII CTTAAG 1 cut(s) 335
AflIII ACRYGT 2 cut(s) 691, 744
AgsI TTSAA 4 cut(s) 56, 125, 517, 784
AjnI CCWGG 1 cut(s) 489
AluBI AGCT 6 cut(s) 49, 59, 90, 181, 277, 328
AluI AGCT 6 cut(s) 49, 59, 90, 181, 277, 328
Alw21I GWGCWC 1 cut(s) 183
AlwI GGATC 1 cut(s) 348
AoxI GGCC 3 cut(s) 157, 633, 639
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 194, 335, 559
AvaII GGWCC 1 cut(s) 247
BalI TGGCCA 1 cut(s) 635
BanII GRGCYC 1 cut(s) 183
BarI GAAGNNNNNNTAC 2 cut(s) 291, 323
Bbv12I GWGCWC 1 cut(s) 183
BceAI ACGGC 1 cut(s) 471
BciT130I CCWGG 1 cut(s) 491
BfaI CTAG 3 cut(s) 27, 128, 531
BfmI CTRYAG 1 cut(s) 481
BfrI CTTAAG 1 cut(s) 335
BisI GCNGC 2 cut(s) 160, 444
BlsI GCNGC 2 cut(s) 161, 445
Bme1390I CCNGG 1 cut(s) 491
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 3 cut(s) 77, 425, 495
BmrFI CCNGG 1 cut(s) 491
BmrI ACTGGG 1 cut(s) 213
BmsI GCATC 4 cut(s) 267, 511, 640, 760
BmuI ACTGGG 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 756
BpuEI CTTGAG 1 cut(s) 45
BsaBI GATNNNNATC 1 cut(s) 595
BsaJI CCNNGG 4 cut(s) 401, 489, 525, 636
BsaXI ACNNNNNCTCC 2 cut(s) 669, 699
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 72, 337, 694
Bse1I ACTGG 2 cut(s) 219, 291
Bse8I GATNNNNATC 1 cut(s) 595
BseBI CCWGG 1 cut(s) 491
BseDI CCNNGG 4 cut(s) 401, 489, 525, 636
BseGI GGATG 2 cut(s) 295, 471
BseJI GATNNNNATC 1 cut(s) 595
BseLI CCNNNNNNNGG 4 cut(s) 22, 72, 337, 694
BseMII CTCAG 2 cut(s) 168, 338
BseNI ACTGG 2 cut(s) 219, 291
BseRI GAGGAG 1 cut(s) 320
BseYI CCCAGC 1 cut(s) 45
BsgI GTGCAG 1 cut(s) 655
BshFI GGCC 3 cut(s) 159, 635, 641
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 2 cut(s) 255, 764
BslFI GGGAC 2 cut(s) 491, 755
BslI CCNNNNNNNGG 4 cut(s) 22, 72, 337, 694
BsmFI GGGAC 2 cut(s) 491, 755
BsmI GAATGC 2 cut(s) 37, 653
BsnI GGCC 3 cut(s) 159, 635, 641
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 3 cut(s) 340, 682, 703
Bsp19I CCATGG 2 cut(s) 401, 525
BspACI CCGC 3 cut(s) 160, 229, 443
BspANI GGCC 3 cut(s) 159, 635, 641
BspCNI CTCAG 2 cut(s) 169, 337
BspLI GGNNCC 3 cut(s) 77, 425, 495
BspPI GGATC 1 cut(s) 348
BspQI GCTCTTC 1 cut(s) 27
BspTI CTTAAG 1 cut(s) 335
BsrI ACTGG 2 cut(s) 219, 291
BssECI CCNNGG 4 cut(s) 401, 489, 525, 636
BssMI GATC 3 cut(s) 340, 682, 703
BssNAI GTATAC 1 cut(s) 134
BssT1I CCWWGG 3 cut(s) 401, 525, 636
Bst1107I GTATAC 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 491
Bst4CI ACNGT 1 cut(s) 676
Bst6I CTCTTC 1 cut(s) 27
BstAFI CTTAAG 1 cut(s) 335
BstDEI CTNAG 2 cut(s) 177, 324
BstDSI CCRYGG 2 cut(s) 401, 525
BstEII GGTNACC 1 cut(s) 547
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 2 cut(s) 295, 471
BstKTI GATC 3 cut(s) 343, 685, 706
BstMBI GATC 3 cut(s) 340, 682, 703
BstNI CCWGG 1 cut(s) 491
BstNSI RCATGY 2 cut(s) 695, 748
BstPI GGTNACC 1 cut(s) 547
BstSCI CCNGG 1 cut(s) 489
BstSFI CTRYAG 1 cut(s) 481
BstZ17I GTATAC 1 cut(s) 134
BsuRI GGCC 3 cut(s) 159, 635, 641
BtgI CCRYGG 2 cut(s) 401, 525
BtsCI GGATG 2 cut(s) 295, 471
BtsIMutI CAGTG 1 cut(s) 226
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 621, 677
CviAII CATG 7 cut(s) 103, 155, 235, 402, 526, 692, 745
CviQI GTAC 2 cut(s) 621, 677
DdeI CTNAG 2 cut(s) 177, 324
DpnI GATC 3 cut(s) 342, 684, 705
DpnII GATC 3 cut(s) 340, 682, 703
EaeI YGGCCR 2 cut(s) 157, 633
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Ecl136II GAGCTC 1 cut(s) 181
Eco130I CCWWGG 3 cut(s) 401, 525, 636
Eco24I GRGCYC 1 cut(s) 183
Eco32I GATATC 1 cut(s) 142
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 334
Eco91I GGTNACC 1 cut(s) 547
EcoICRI GAGCTC 1 cut(s) 181
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 547
EcoRII CCWGG 1 cut(s) 489
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 3 cut(s) 401, 525, 636
EcoT22I ATGCAT 2 cut(s) 655, 793
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 3 cut(s) 401, 525, 636
FaeI CATG 7 cut(s) 106, 158, 238, 405, 529, 695, 748
FaqI GGGAC 2 cut(s) 491, 755
FatI CATG 7 cut(s) 102, 154, 234, 401, 525, 691, 744
FauNDI CATATG 2 cut(s) 71, 460
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 2 cut(s) 160, 444
FokI GGATG 2 cut(s) 302, 478
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 2 cut(s) 160, 444
FspBI CTAG 3 cut(s) 27, 128, 531
GluI GCNGC 2 cut(s) 160, 444
GsaI CCCAGC 1 cut(s) 49
GsuI CTGGAG 1 cut(s) 756
HaeIII GGCC 3 cut(s) 159, 635, 641
HapII CCGG 2 cut(s) 255, 764
HgaI GACGC 1 cut(s) 326
Hin1II CATG 7 cut(s) 106, 158, 238, 405, 529, 695, 748
HpaII CCGG 2 cut(s) 255, 764
HphI GGTGA 3 cut(s) 194, 335, 559
Hpy166II GTNNAC 3 cut(s) 134, 226, 250
Hpy188I TCNGA 1 cut(s) 178
Hpy8I GTNNAC 3 cut(s) 134, 226, 250
HpyAV CCTTC 2 cut(s) 293, 358
HpyCH4III ACNGT 1 cut(s) 676
HpyCH4V TGCA 9 cut(s) 119, 191, 266, 573, 653, 672, 709, 773, 791
HpyF3I CTNAG 2 cut(s) 177, 324
Hsp92II CATG 7 cut(s) 106, 158, 238, 405, 529, 695, 748
Kzo9I GATC 3 cut(s) 340, 682, 703
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 2 cut(s) 333, 493
LweI GCATC 4 cut(s) 267, 511, 640, 760
MaeI CTAG 3 cut(s) 27, 128, 531
MaeIII GTNAC 3 cut(s) 391, 547, 558
MalI GATC 3 cut(s) 342, 684, 705
MboI GATC 3 cut(s) 340, 682, 703
MboII GAAGA 3 cut(s) 44, 44, 308
MfeI CAATTG 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 183
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 4 cut(s) 120, 198, 663, 774
MluNI TGGCCA 1 cut(s) 635
MnlI CCTC 8 cut(s) 119, 242, 286, 341, 580, 731, 745, 771
Mox20I TGGCCA 1 cut(s) 635
Mph1103I ATGCAT 2 cut(s) 655, 793
MscI TGGCCA 1 cut(s) 635
MseI TTAA 4 cut(s) 336, 450, 564, 593
Msp20I TGGCCA 1 cut(s) 635
MspCI CTTAAG 1 cut(s) 335
MspI CCGG 2 cut(s) 255, 764
MspR9I CCNGG 1 cut(s) 491
MunI CAATTG 1 cut(s) 120
Mva1269I GAATGC 2 cut(s) 37, 653
MvaI CCWGG 1 cut(s) 491
NcoI CCATGG 2 cut(s) 401, 525
NdeI CATATG 2 cut(s) 71, 460
NdeII GATC 3 cut(s) 340, 682, 703
NlaIII CATG 7 cut(s) 106, 158, 238, 405, 529, 695, 748
NlaIV GGNNCC 3 cut(s) 77, 425, 495
NmuCI GTSAC 2 cut(s) 391, 547
NsiI ATGCAT 2 cut(s) 655, 793
NspI RCATGY 2 cut(s) 695, 748
PciI ACATGT 2 cut(s) 691, 744
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 2 cut(s) 37, 653
PkrI GCNGC 2 cut(s) 161, 445
PscI ACATGT 2 cut(s) 691, 744
Psp124BI GAGCTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 489
PspEI GGTNACC 1 cut(s) 547
PspFI CCCAGC 1 cut(s) 45
PspGI CCWGG 1 cut(s) 489
PspN4I GGNNCC 3 cut(s) 77, 425, 495
PspPI GGNCC 1 cut(s) 247
PsrI GAACNNNNNNTAC 2 cut(s) 117, 149
RsaI GTAC 2 cut(s) 622, 678
RsaNI GTAC 2 cut(s) 621, 677
SacI GAGCTC 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 4 cut(s) 336, 450, 564, 593
SatI GCNGC 2 cut(s) 160, 444
Sau3AI GATC 3 cut(s) 340, 682, 703
Sau96I GGNCC 1 cut(s) 247
ScrFI CCNGG 1 cut(s) 491
SduI GDGCHC 1 cut(s) 183
SetI ASST 9 cut(s) 51, 61, 92, 133, 183, 279, 304, 330, 726
SfaNI GCATC 4 cut(s) 267, 511, 640, 760
SfcI CTRYAG 1 cut(s) 481
SinI GGWCC 1 cut(s) 247
SmlI CTYRAG 2 cut(s) 60, 335
SmoI CTYRAG 2 cut(s) 60, 335
Sse9I AATT 4 cut(s) 120, 198, 663, 774
SsiI CCGC 3 cut(s) 160, 229, 443
SspMI CTAG 3 cut(s) 27, 128, 531
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 1 cut(s) 489
StyI CCWWGG 3 cut(s) 401, 525, 636
TaaI ACNGT 1 cut(s) 676
TaqI TCGA 1 cut(s) 585
TasI AATT 4 cut(s) 120, 198, 663, 774
TauI GCSGC 2 cut(s) 162, 446
Tru1I TTAA 4 cut(s) 336, 450, 564, 593
Tru9I TTAA 4 cut(s) 336, 450, 564, 593
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 2 cut(s) 391, 547
Tsp45I GTSAC 2 cut(s) 391, 547
TspDTI ATGAA 2 cut(s) 82, 428
TspRI CASTG 1 cut(s) 226
Vha464I CTTAAG 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 335
XceI RCATGY 2 cut(s) 695, 748
XmiI GTMKAC 1 cut(s) 133
XspI CTAG 3 cut(s) 27, 128, 531
Zsp2I ATGCAT 2 cut(s) 655, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.