Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 729 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 417 |
| AccI | GTMKAC | 2 cut(s) | 151, 243 |
| AciI | CCGC | 1 cut(s) | 247 |
| AclWI | GGATC | 1 cut(s) | 363 |
| AcuI | CTGAAG | 2 cut(s) | 88, 371 |
| AfaI | GTAC | 2 cut(s) | 637, 693 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 90, 352, 417, 709, 1070 |
| AflII | CTTAAG | 2 cut(s) | 364, 845 |
| AflIII | ACRYGT | 2 cut(s) | 706, 759 |
| AgsI | TTSAA | 5 cut(s) | 143, 532, 683, 742, 947 |
| AleI | CACNNNNGTG | 1 cut(s) | 569 |
| AluBI | AGCT | 8 cut(s) | 18, 67, 108, 199, 295, 343, 955, 1024 |
| AluI | AGCT | 8 cut(s) | 18, 67, 108, 199, 295, 343, 955, 1024 |
| Alw21I | GWGCWC | 3 cut(s) | 201, 270, 434 |
| Alw26I | GTCTC | 1 cut(s) | 1086 |
| Alw44I | GTGCAC | 1 cut(s) | 266 |
| AlwI | GGATC | 1 cut(s) | 363 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 644 |
| AoxI | GGCC | 2 cut(s) | 654, 978 |
| ApaLI | GTGCAC | 1 cut(s) | 266 |
| ApeKI | GCWGC | 1 cut(s) | 952 |
| AspS9I | GGNCC | 1 cut(s) | 979 |
| AsuHPI | GGTGA | 4 cut(s) | 350, 556, 767, 904 |
| AsuNHI | GCTAGC | 1 cut(s) | 545 |
| BaeGI | GKGCMC | 1 cut(s) | 270 |
| BanII | GRGCYC | 1 cut(s) | 201 |
| Bbv12I | GWGCWC | 3 cut(s) | 201, 270, 434 |
| BbvI | GCAGC | 1 cut(s) | 964 |
| BccI | CCATC | 3 cut(s) | 513, 907, 970 |
| BclI | TGATCA | 1 cut(s) | 573 |
| BcoDI | GTCTC | 1 cut(s) | 1086 |
| BfaI | CTAG | 3 cut(s) | 146, 546, 855 |
| BfrI | CTTAAG | 2 cut(s) | 364, 845 |
| BfuAI | ACCTGC | 1 cut(s) | 729 |
| BisI | GCNGC | 1 cut(s) | 953 |
| BlpI | GCTNAGC | 1 cut(s) | 1020 |
| BlsI | GCNGC | 1 cut(s) | 954 |
| BmgT120I | GGNCC | 1 cut(s) | 979 |
| BmiI | GGNNCC | 3 cut(s) | 95, 440, 503 |
| BmsI | GCATC | 5 cut(s) | 322, 526, 541, 655, 1024 |
| BmtI | GCTAGC | 1 cut(s) | 549 |
| BpmI | CTGGAG | 1 cut(s) | 771 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1020 |
| BpuEI | CTTGAG | 3 cut(s) | 63, 448, 782 |
| Bsa29I | ATCGAT | 1 cut(s) | 579 |
| BsaI | GGTCTC | 1 cut(s) | 1086 |
| BsaJI | CCNNGG | 2 cut(s) | 416, 540 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 684, 714 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 90, 352, 417, 709, 1070 |
| Bse1I | ACTGG | 2 cut(s) | 754, 821 |
| Bse3DI | GCAATG | 1 cut(s) | 657 |
| BseCI | ATCGAT | 1 cut(s) | 579 |
| BseDI | CCNNGG | 2 cut(s) | 416, 540 |
| BseGI | GGATG | 1 cut(s) | 486 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 90, 352, 417, 709, 1070 |
| BseMI | GCAATG | 1 cut(s) | 657 |
| BseMII | CTCAG | 2 cut(s) | 186, 287 |
| BseNI | ACTGG | 2 cut(s) | 754, 821 |
| BseSI | GKGCMC | 1 cut(s) | 270 |
| BseXI | GCAGC | 1 cut(s) | 964 |
| BseYI | CCCAGC | 1 cut(s) | 339 |
| BsgI | GTGCAG | 1 cut(s) | 192 |
| BshFI | GGCC | 2 cut(s) | 656, 980 |
| BshVI | ATCGAT | 1 cut(s) | 579 |
| BsiHKAI | GWGCWC | 3 cut(s) | 201, 270, 434 |
| BslFI | GGGAC | 1 cut(s) | 109 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 90, 352, 417, 709, 1070 |
| BsmAI | GTCTC | 1 cut(s) | 1086 |
| BsmFI | GGGAC | 1 cut(s) | 109 |
| BsmI | GAATGC | 3 cut(s) | 55, 668, 689 |
| BsnI | GGCC | 2 cut(s) | 656, 980 |
| Bso31I | GGTCTC | 1 cut(s) | 1086 |
| Bsp1286I | GDGCHC | 3 cut(s) | 201, 270, 434 |
| Bsp143I | GATC | 4 cut(s) | 355, 573, 697, 851 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1020 |
| Bsp19I | CCATGG | 2 cut(s) | 416, 540 |
| BspACI | CCGC | 1 cut(s) | 247 |
| BspANI | GGCC | 2 cut(s) | 656, 980 |
| BspCNI | CTCAG | 2 cut(s) | 187, 288 |
| BspDI | ATCGAT | 1 cut(s) | 579 |
| BspLI | GGNNCC | 3 cut(s) | 95, 440, 503 |
| BspMI | ACCTGC | 1 cut(s) | 729 |
| BspOI | GCTAGC | 1 cut(s) | 549 |
| BspPI | GGATC | 1 cut(s) | 363 |
| BspQI | GCTCTTC | 1 cut(s) | 1031 |
| BspTI | CTTAAG | 2 cut(s) | 364, 845 |
| BspTNI | GGTCTC | 1 cut(s) | 1086 |
| BsrDI | GCAATG | 1 cut(s) | 657 |
| BsrI | ACTGG | 2 cut(s) | 754, 821 |
| BssECI | CCNNGG | 2 cut(s) | 416, 540 |
| BssMI | GATC | 4 cut(s) | 355, 573, 697, 851 |
| BssNAI | GTATAC | 1 cut(s) | 152 |
| BssT1I | CCWWGG | 2 cut(s) | 416, 540 |
| Bst1107I | GTATAC | 1 cut(s) | 152 |
| Bst4CI | ACNGT | 3 cut(s) | 646, 691, 790 |
| Bst6I | CTCTTC | 1 cut(s) | 1031 |
| BstAFI | CTTAAG | 2 cut(s) | 364, 845 |
| BstC8I | GCNNGC | 1 cut(s) | 547 |
| BstDEI | CTNAG | 4 cut(s) | 195, 285, 296, 1020 |
| BstDSI | CCRYGG | 2 cut(s) | 416, 540 |
| BstEII | GGTNACC | 1 cut(s) | 562 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 350 |
| BstF5I | GGATG | 1 cut(s) | 486 |
| BstKTI | GATC | 4 cut(s) | 358, 576, 700, 854 |
| BstMAI | GTCTC | 1 cut(s) | 1086 |
| BstMBI | GATC | 4 cut(s) | 355, 573, 697, 851 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 246, 551 |
| BstNSI | RCATGY | 3 cut(s) | 710, 763, 970 |
| BstPI | GGTNACC | 1 cut(s) | 562 |
| BstSLI | GKGCMC | 1 cut(s) | 270 |
| BstV1I | GCAGC | 1 cut(s) | 964 |
| BstZ17I | GTATAC | 1 cut(s) | 152 |
| Bsu15I | ATCGAT | 1 cut(s) | 579 |
| BsuRI | GGCC | 2 cut(s) | 656, 980 |
| BsuTUI | ATCGAT | 1 cut(s) | 579 |
| BtgI | CCRYGG | 2 cut(s) | 416, 540 |
| BtgZI | GCGATG | 2 cut(s) | 564, 945 |
| BtsCI | GGATG | 1 cut(s) | 486 |
| BtsIMutI | CAGTG | 3 cut(s) | 687, 1029, 1051 |
| BveI | ACCTGC | 1 cut(s) | 729 |
| Cac8I | GCNNGC | 1 cut(s) | 547 |
| CaiI | CAGNNNCTG | 1 cut(s) | 644 |
| Cfr13I | GGNCC | 1 cut(s) | 979 |
| ClaI | ATCGAT | 1 cut(s) | 579 |
| CseI | GACGC | 1 cut(s) | 229 |
| Csp6I | GTAC | 2 cut(s) | 636, 692 |
| CviQI | GTAC | 2 cut(s) | 636, 692 |
| DdeI | CTNAG | 4 cut(s) | 195, 285, 296, 1020 |
| DpnI | GATC | 4 cut(s) | 357, 575, 699, 853 |
| DpnII | GATC | 4 cut(s) | 355, 573, 697, 851 |
| Eam1104I | CTCTTC | 1 cut(s) | 1031 |
| EarI | CTCTTC | 1 cut(s) | 1031 |
| Ecl136II | GAGCTC | 1 cut(s) | 199 |
| Eco130I | CCWWGG | 2 cut(s) | 416, 540 |
| Eco24I | GRGCYC | 1 cut(s) | 201 |
| Eco31I | GGTCTC | 1 cut(s) | 1086 |
| Eco32I | GATATC | 1 cut(s) | 160 |
| Eco53kI | GAGCTC | 1 cut(s) | 199 |
| Eco57I | CTGAAG | 2 cut(s) | 88, 371 |
| Eco91I | GGTNACC | 1 cut(s) | 562 |
| EcoICRI | GAGCTC | 1 cut(s) | 199 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 350 |
| EcoO65I | GGTNACC | 1 cut(s) | 562 |
| EcoRV | GATATC | 1 cut(s) | 160 |
| EcoT14I | CCWWGG | 2 cut(s) | 416, 540 |
| EcoT22I | ATGCAT | 1 cut(s) | 670 |
| EcoT38I | GRGCYC | 1 cut(s) | 201 |
| ErhI | CCWWGG | 2 cut(s) | 416, 540 |
| FaqI | GGGAC | 1 cut(s) | 109 |
| FbaI | TGATCA | 1 cut(s) | 573 |
| FblI | GTMKAC | 2 cut(s) | 151, 243 |
| Fnu4HI | GCNGC | 1 cut(s) | 953 |
| FokI | GGATG | 1 cut(s) | 493 |
| FriOI | GRGCYC | 1 cut(s) | 201 |
| Fsp4HI | GCNGC | 1 cut(s) | 953 |
| FspBI | CTAG | 3 cut(s) | 146, 546, 855 |
| GluI | GCNGC | 1 cut(s) | 953 |
| GsaI | CCCAGC | 1 cut(s) | 343 |
| GsuI | CTGGAG | 1 cut(s) | 771 |
| HaeIII | GGCC | 2 cut(s) | 656, 980 |
| HgaI | GACGC | 1 cut(s) | 229 |
| HinfI | GANTC | 3 cut(s) | 187, 611, 938 |
| HphI | GGTGA | 4 cut(s) | 350, 556, 767, 904 |
| Hpy166II | GTNNAC | 3 cut(s) | 152, 244, 268 |
| Hpy188I | TCNGA | 2 cut(s) | 196, 351 |
| Hpy188III | TCNNGA | 2 cut(s) | 91, 855 |
| Hpy8I | GTNNAC | 3 cut(s) | 152, 244, 268 |
| HpyAV | CCTTC | 4 cut(s) | 308, 346, 356, 388 |
| HpyCH4III | ACNGT | 3 cut(s) | 646, 691, 790 |
| HpyCH4V | TGCA | 8 cut(s) | 137, 209, 268, 588, 668, 687, 724, 811 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 246, 551 |
| HpyF3I | CTNAG | 4 cut(s) | 195, 285, 296, 1020 |
| Ksp22I | TGATCA | 1 cut(s) | 573 |
| Kzo9I | GATC | 4 cut(s) | 355, 573, 697, 851 |
| LguI | GCTCTTC | 1 cut(s) | 1031 |
| LmnI | GCTCC | 1 cut(s) | 348 |
| Lsp1109I | GCAGC | 1 cut(s) | 964 |
| LweI | GCATC | 5 cut(s) | 322, 526, 541, 655, 1024 |
| MaeI | CTAG | 3 cut(s) | 146, 546, 855 |
| MaeIII | GTNAC | 4 cut(s) | 562, 755, 985, 1046 |
| MalI | GATC | 4 cut(s) | 357, 575, 699, 853 |
| MboI | GATC | 4 cut(s) | 355, 573, 697, 851 |
| MboII | GAAGA | 5 cut(s) | 94, 520, 839, 952, 1018 |
| MfeI | CAATTG | 1 cut(s) | 942 |
| MhlI | GDGCHC | 3 cut(s) | 201, 270, 434 |
| MluCI | AATT | 4 cut(s) | 138, 421, 864, 942 |
| MlyI | GAGTC | 2 cut(s) | 181, 620 |
| MmeI | TCCRAC | 2 cut(s) | 84, 607 |
| Mph1103I | ATGCAT | 1 cut(s) | 670 |
| MseI | TTAA | 5 cut(s) | 30, 365, 383, 846, 1076 |
| MslI | CAYNNNNRTG | 3 cut(s) | 569, 905, 971 |
| MspA1I | CMGCKG | 1 cut(s) | 955 |
| MspCI | CTTAAG | 2 cut(s) | 364, 845 |
| MunI | CAATTG | 1 cut(s) | 942 |
| Mva1269I | GAATGC | 3 cut(s) | 55, 668, 689 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 246, 551 |
| NcoI | CCATGG | 2 cut(s) | 416, 540 |
| NdeII | GATC | 4 cut(s) | 355, 573, 697, 851 |
| NheI | GCTAGC | 1 cut(s) | 545 |
| NlaIV | GGNNCC | 3 cut(s) | 95, 440, 503 |
| NmuCI | GTSAC | 4 cut(s) | 562, 755, 985, 1046 |
| NsiI | ATGCAT | 1 cut(s) | 670 |
| NspI | RCATGY | 3 cut(s) | 710, 763, 970 |
| OliI | CACNNNNGTG | 1 cut(s) | 569 |
| PciI | ACATGT | 2 cut(s) | 706, 759 |
| PciSI | GCTCTTC | 1 cut(s) | 1031 |
| PctI | GAATGC | 3 cut(s) | 55, 668, 689 |
| PfeI | GAWTC | 1 cut(s) | 938 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 417 |
| PkrI | GCNGC | 1 cut(s) | 954 |
| PleI | GAGTC | 2 cut(s) | 181, 619 |
| PpsI | GAGTC | 2 cut(s) | 181, 619 |
| PscI | ACATGT | 2 cut(s) | 706, 759 |
| Psp124BI | GAGCTC | 1 cut(s) | 201 |
| PspEI | GGTNACC | 1 cut(s) | 562 |
| PspFI | CCCAGC | 1 cut(s) | 339 |
| PspN4I | GGNNCC | 3 cut(s) | 95, 440, 503 |
| PspPI | GGNCC | 1 cut(s) | 979 |
| PstNI | CAGNNNCTG | 1 cut(s) | 644 |
| PvuII | CAGCTG | 1 cut(s) | 955 |
| RsaI | GTAC | 2 cut(s) | 637, 693 |
| RsaNI | GTAC | 2 cut(s) | 636, 692 |
| RseI | CAYNNNNRTG | 3 cut(s) | 569, 905, 971 |
| SacI | GAGCTC | 1 cut(s) | 201 |
| SapI | GCTCTTC | 1 cut(s) | 1031 |
| SaqAI | TTAA | 5 cut(s) | 30, 365, 383, 846, 1076 |
| SatI | GCNGC | 1 cut(s) | 953 |
| Sau3AI | GATC | 4 cut(s) | 355, 573, 697, 851 |
| Sau96I | GGNCC | 1 cut(s) | 979 |
| SchI | GAGTC | 2 cut(s) | 181, 620 |
| SduI | GDGCHC | 3 cut(s) | 201, 270, 434 |
| SfaNI | GCATC | 5 cut(s) | 322, 526, 541, 655, 1024 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 569, 905, 971 |
| SmlI | CTYRAG | 5 cut(s) | 78, 364, 427, 797, 845 |
| SmoI | CTYRAG | 5 cut(s) | 78, 364, 427, 797, 845 |
| Sse9I | AATT | 4 cut(s) | 138, 421, 864, 942 |
| SsiI | CCGC | 1 cut(s) | 247 |
| SspI | AATATT | 1 cut(s) | 261 |
| SspMI | CTAG | 3 cut(s) | 146, 546, 855 |
| SstI | GAGCTC | 1 cut(s) | 201 |
| StyI | CCWWGG | 2 cut(s) | 416, 540 |
| TaaI | ACNGT | 3 cut(s) | 646, 691, 790 |
| TaqI | TCGA | 4 cut(s) | 396, 579, 621, 963 |
| TasI | AATT | 4 cut(s) | 138, 421, 864, 942 |
| TatI | WGTACW | 1 cut(s) | 691 |
| TfiI | GAWTC | 1 cut(s) | 938 |
| Tru1I | TTAA | 5 cut(s) | 30, 365, 383, 846, 1076 |
| Tru9I | TTAA | 5 cut(s) | 30, 365, 383, 846, 1076 |
| TscAI | CASTG | 3 cut(s) | 694, 1036, 1051 |
| TseFI | GTSAC | 4 cut(s) | 562, 755, 985, 1046 |
| TseI | GCWGC | 1 cut(s) | 952 |
| Tsp45I | GTSAC | 4 cut(s) | 562, 755, 985, 1046 |
| TspDTI | ATGAA | 3 cut(s) | 62, 100, 840 |
| TspRI | CASTG | 3 cut(s) | 694, 1036, 1051 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 417 |
| Vha464I | CTTAAG | 2 cut(s) | 364, 845 |
| VneI | GTGCAC | 1 cut(s) | 266 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 350 |
| XbaI | TCTAGA | 1 cut(s) | 854 |
| XceI | RCATGY | 3 cut(s) | 710, 763, 970 |
| XmiI | GTMKAC | 2 cut(s) | 151, 243 |
| XspI | CTAG | 3 cut(s) | 146, 546, 855 |
| Zsp2I | ATGCAT | 1 cut(s) | 670 |