Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1037 |
| AccII | CGCG | 1 cut(s) | 350 |
| AciI | CCGC | 4 cut(s) | 315, 348, 639, 788 |
| AclWI | GGATC | 1 cut(s) | 416 |
| AcoI | YGGCCR | 3 cut(s) | 187, 657, 789 |
| AcsI | RAATTY | 1 cut(s) | 127 |
| AcvI | CACGTG | 1 cut(s) | 478 |
| AcyI | GRCGYC | 2 cut(s) | 342, 546 |
| AdeI | CACNNNGTG | 1 cut(s) | 481 |
| AfaI | GTAC | 3 cut(s) | 333, 596, 702 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 102, 1079 |
| AflIII | ACRYGT | 1 cut(s) | 768 |
| AgsI | TTSAA | 3 cut(s) | 541, 611, 808 |
| AjiI | CACGTC | 1 cut(s) | 634 |
| AjnI | CCWGG | 1 cut(s) | 513 |
| AluBI | AGCT | 8 cut(s) | 79, 120, 152, 211, 245, 524, 579, 1033 |
| AluI | AGCT | 8 cut(s) | 79, 120, 152, 211, 245, 524, 579, 1033 |
| Alw21I | GWGCWC | 1 cut(s) | 405 |
| Alw26I | GTCTC | 2 cut(s) | 220, 1095 |
| AlwI | GGATC | 1 cut(s) | 416 |
| Ama87I | CYCGRG | 1 cut(s) | 574 |
| AoxI | GGCC | 4 cut(s) | 187, 384, 657, 789 |
| ApeKI | GCWGC | 1 cut(s) | 149 |
| ApoI | RAATTY | 1 cut(s) | 127 |
| AspLEI | GCGC | 2 cut(s) | 352, 380 |
| AspS9I | GGNCC | 2 cut(s) | 384, 517 |
| AsuC2I | CCSGG | 1 cut(s) | 565 |
| AsuHPI | GGTGA | 3 cut(s) | 14, 595, 913 |
| AsuNHI | GCTAGC | 1 cut(s) | 554 |
| AvaI | CYCGRG | 1 cut(s) | 574 |
| AvaII | GGWCC | 1 cut(s) | 517 |
| BalI | TGGCCA | 2 cut(s) | 189, 659 |
| BbrPI | CACGTG | 1 cut(s) | 478 |
| Bbv12I | GWGCWC | 1 cut(s) | 405 |
| BbvI | GCAGC | 1 cut(s) | 161 |
| BccI | CCATC | 2 cut(s) | 419, 979 |
| BciT130I | CCWGG | 1 cut(s) | 515 |
| BciVI | GTATCC | 1 cut(s) | 173 |
| BcnI | CCSGG | 1 cut(s) | 565 |
| BcoDI | GTCTC | 2 cut(s) | 220, 1095 |
| BfaI | CTAG | 2 cut(s) | 297, 555 |
| BfmI | CTRYAG | 1 cut(s) | 996 |
| BfuAI | ACCTGC | 1 cut(s) | 1037 |
| BfuI | GTATCC | 1 cut(s) | 173 |
| BisI | GCNGC | 3 cut(s) | 150, 316, 789 |
| BlpI | GCTNAGC | 1 cut(s) | 1029 |
| BlsI | GCNGC | 3 cut(s) | 151, 317, 790 |
| Bme1390I | CCNGG | 2 cut(s) | 515, 565 |
| Bme18I | GGWCC | 1 cut(s) | 517 |
| BmeT110I | CYCGRG | 1 cut(s) | 574 |
| BmgBI | CACGTC | 1 cut(s) | 634 |
| BmgT120I | GGNCC | 2 cut(s) | 384, 517 |
| BmiI | GGNNCC | 5 cut(s) | 107, 449, 512, 519, 652 |
| BmrFI | CCNGG | 2 cut(s) | 515, 565 |
| BmsI | GCATC | 4 cut(s) | 15, 550, 664, 784 |
| BmtI | GCTAGC | 1 cut(s) | 558 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 200, 232 |
| BpmI | CTGGAG | 1 cut(s) | 847 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1029 |
| BpuEI | CTTGAG | 2 cut(s) | 52, 875 |
| BpuMI | CCSGG | 1 cut(s) | 565 |
| BsaAI | YACGTR | 1 cut(s) | 478 |
| BsaHI | GRCGYC | 2 cut(s) | 342, 546 |
| BsaI | GGTCTC | 1 cut(s) | 1095 |
| BsaJI | CCNNGG | 3 cut(s) | 425, 549, 654 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 693, 723 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 102, 1079 |
| Bse1I | ACTGG | 1 cut(s) | 830 |
| Bse3DI | GCAATG | 1 cut(s) | 666 |
| BseBI | CCWGG | 1 cut(s) | 515 |
| BseDI | CCNNGG | 3 cut(s) | 425, 549, 654 |
| BseGI | GGATG | 3 cut(s) | 174, 252, 495 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 102, 1079 |
| BseMI | GCAATG | 1 cut(s) | 666 |
| BseMII | CTCAG | 2 cut(s) | 198, 299 |
| BseNI | ACTGG | 1 cut(s) | 830 |
| BseRI | GAGGAG | 1 cut(s) | 344 |
| BseX3I | CGGCCG | 1 cut(s) | 789 |
| BseXI | GCAGC | 1 cut(s) | 161 |
| BseYI | CCCAGC | 1 cut(s) | 520 |
| BsgI | GTGCAG | 1 cut(s) | 168 |
| Bsh1236I | CGCG | 1 cut(s) | 350 |
| Bsh1285I | CGRYCG | 1 cut(s) | 792 |
| BshFI | GGCC | 4 cut(s) | 189, 386, 659, 791 |
| BsiEI | CGRYCG | 1 cut(s) | 792 |
| BsiHKAI | GWGCWC | 1 cut(s) | 405 |
| BsiHKCI | CYCGRG | 1 cut(s) | 574 |
| BsiSI | CCGG | 2 cut(s) | 285, 565 |
| BslFI | GGGAC | 3 cut(s) | 121, 658, 779 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 102, 1079 |
| BsmAI | GTCTC | 2 cut(s) | 220, 1095 |
| BsmFI | GGGAC | 3 cut(s) | 121, 658, 779 |
| BsnI | GGCC | 4 cut(s) | 189, 386, 659, 791 |
| Bso31I | GGTCTC | 1 cut(s) | 1095 |
| BsoBI | CYCGRG | 1 cut(s) | 574 |
| Bsp1286I | GDGCHC | 1 cut(s) | 405 |
| Bsp143I | GATC | 3 cut(s) | 421, 570, 706 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1029 |
| Bsp19I | CCATGG | 3 cut(s) | 425, 549, 654 |
| BspACI | CCGC | 4 cut(s) | 315, 348, 639, 788 |
| BspANI | GGCC | 4 cut(s) | 189, 386, 659, 791 |
| BspCNI | CTCAG | 2 cut(s) | 199, 300 |
| BspFNI | CGCG | 1 cut(s) | 350 |
| BspHI | TCATGA | 1 cut(s) | 73 |
| BspLI | GGNNCC | 5 cut(s) | 107, 449, 512, 519, 652 |
| BspMI | ACCTGC | 1 cut(s) | 1037 |
| BspOI | GCTAGC | 1 cut(s) | 558 |
| BspPI | GGATC | 1 cut(s) | 416 |
| BspTNI | GGTCTC | 1 cut(s) | 1095 |
| BsrDI | GCAATG | 1 cut(s) | 666 |
| BsrI | ACTGG | 1 cut(s) | 830 |
| BssECI | CCNNGG | 3 cut(s) | 425, 549, 654 |
| BssMI | GATC | 3 cut(s) | 421, 570, 706 |
| BssNI | GRCGYC | 2 cut(s) | 342, 546 |
| BssT1I | CCWWGG | 3 cut(s) | 425, 549, 654 |
| Bst2UI | CCWGG | 1 cut(s) | 515 |
| Bst4CI | ACNGT | 2 cut(s) | 700, 922 |
| BstACI | GRCGYC | 2 cut(s) | 342, 546 |
| BstBAI | YACGTR | 1 cut(s) | 478 |
| BstC8I | GCNNGC | 2 cut(s) | 191, 556 |
| BstDEI | CTNAG | 4 cut(s) | 207, 274, 308, 1029 |
| BstDSI | CCRYGG | 3 cut(s) | 425, 549, 654 |
| BstF5I | GGATG | 3 cut(s) | 174, 252, 495 |
| BstFNI | CGCG | 1 cut(s) | 350 |
| BstHHI | GCGC | 2 cut(s) | 352, 380 |
| BstKTI | GATC | 3 cut(s) | 424, 573, 709 |
| BstMAI | GTCTC | 2 cut(s) | 220, 1095 |
| BstMBI | GATC | 3 cut(s) | 421, 570, 706 |
| BstMCI | CGRYCG | 1 cut(s) | 792 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 560, 785, 794, 894 |
| BstNI | CCWGG | 1 cut(s) | 515 |
| BstNSI | RCATGY | 1 cut(s) | 772 |
| BstSCI | CCNGG | 2 cut(s) | 513, 563 |
| BstSFI | CTRYAG | 1 cut(s) | 996 |
| BstUI | CGCG | 1 cut(s) | 350 |
| BstV1I | GCAGC | 1 cut(s) | 161 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 185 |
| BstZI | CGGCCG | 1 cut(s) | 789 |
| BsuI | GTATCC | 1 cut(s) | 173 |
| BsuRI | GGCC | 4 cut(s) | 189, 386, 659, 791 |
| BtgI | CCRYGG | 3 cut(s) | 425, 549, 654 |
| BtgZI | GCGATG | 1 cut(s) | 573 |
| BtrI | CACGTC | 1 cut(s) | 634 |
| BtsCI | GGATG | 3 cut(s) | 174, 252, 495 |
| BtsIMutI | CAGTG | 1 cut(s) | 696 |
| BveI | ACCTGC | 1 cut(s) | 1037 |
| Cac8I | GCNNGC | 2 cut(s) | 191, 556 |
| CciI | TCATGA | 1 cut(s) | 73 |
| CfoI | GCGC | 2 cut(s) | 352, 380 |
| Cfr13I | GGNCC | 2 cut(s) | 384, 517 |
| CseI | GACGC | 3 cut(s) | 350, 554, 1052 |
| Csp6I | GTAC | 3 cut(s) | 332, 595, 701 |
| CviQI | GTAC | 3 cut(s) | 332, 595, 701 |
| DdeI | CTNAG | 4 cut(s) | 207, 274, 308, 1029 |
| DpnI | GATC | 3 cut(s) | 423, 572, 708 |
| DpnII | GATC | 3 cut(s) | 421, 570, 706 |
| DraIII | CACNNNGTG | 1 cut(s) | 481 |
| EaeI | YGGCCR | 3 cut(s) | 187, 657, 789 |
| EagI | CGGCCG | 1 cut(s) | 789 |
| EclXI | CGGCCG | 1 cut(s) | 789 |
| Eco130I | CCWWGG | 3 cut(s) | 425, 549, 654 |
| Eco31I | GGTCTC | 1 cut(s) | 1095 |
| Eco47I | GGWCC | 1 cut(s) | 517 |
| Eco52I | CGGCCG | 1 cut(s) | 789 |
| Eco72I | CACGTG | 1 cut(s) | 478 |
| Eco88I | CYCGRG | 1 cut(s) | 574 |
| EcoO109I | RGGNCCY | 2 cut(s) | 384, 517 |
| EcoRII | CCWGG | 1 cut(s) | 513 |
| EcoT14I | CCWWGG | 3 cut(s) | 425, 549, 654 |
| ErhI | CCWWGG | 3 cut(s) | 425, 549, 654 |
| FaqI | GGGAC | 3 cut(s) | 121, 658, 779 |
| FauNDI | CATATG | 1 cut(s) | 687 |
| Fnu4HI | GCNGC | 3 cut(s) | 150, 316, 789 |
| FokI | GGATG | 3 cut(s) | 161, 239, 502 |
| Fsp4HI | GCNGC | 3 cut(s) | 150, 316, 789 |
| FspBI | CTAG | 2 cut(s) | 297, 555 |
| GlaI | GCGC | 2 cut(s) | 351, 379 |
| GluI | GCNGC | 3 cut(s) | 150, 316, 789 |
| GsaI | CCCAGC | 1 cut(s) | 524 |
| GsuI | CTGGAG | 1 cut(s) | 847 |
| HaeIII | GGCC | 4 cut(s) | 189, 386, 659, 791 |
| HapII | CCGG | 2 cut(s) | 285, 565 |
| HgaI | GACGC | 3 cut(s) | 350, 554, 1052 |
| HhaI | GCGC | 2 cut(s) | 352, 380 |
| Hin1I | GRCGYC | 2 cut(s) | 342, 546 |
| Hin6I | GCGC | 2 cut(s) | 350, 378 |
| HinP1I | GCGC | 2 cut(s) | 350, 378 |
| HincII | GTYRAC | 1 cut(s) | 631 |
| HindII | GTYRAC | 1 cut(s) | 631 |
| HinfI | GANTC | 4 cut(s) | 199, 270, 620, 947 |
| HpaII | CCGG | 2 cut(s) | 285, 565 |
| HphI | GGTGA | 3 cut(s) | 14, 595, 913 |
| Hpy166II | GTNNAC | 2 cut(s) | 280, 631 |
| Hpy188I | TCNGA | 2 cut(s) | 208, 309 |
| Hpy188III | TCNNGA | 4 cut(s) | 31, 74, 358, 574 |
| Hpy8I | GTNNAC | 2 cut(s) | 280, 631 |
| HpyAV | CCTTC | 2 cut(s) | 397, 688 |
| HpyCH4III | ACNGT | 2 cut(s) | 700, 922 |
| HpyCH4IV | ACGT | 2 cut(s) | 477, 633 |
| HpyCH4V | TGCA | 7 cut(s) | 6, 149, 484, 530, 696, 761, 820 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 560, 785, 794, 894 |
| HpyF3I | CTNAG | 4 cut(s) | 207, 274, 308, 1029 |
| HpySE526I | ACGT | 2 cut(s) | 477, 633 |
| Hsp92I | GRCGYC | 2 cut(s) | 342, 546 |
| HspAI | GCGC | 2 cut(s) | 350, 378 |
| Kzo9I | GATC | 3 cut(s) | 421, 570, 706 |
| LmnI | GCTCC | 1 cut(s) | 357 |
| Lsp1109I | GCAGC | 1 cut(s) | 161 |
| LweI | GCATC | 4 cut(s) | 15, 550, 664, 784 |
| MaeI | CTAG | 2 cut(s) | 297, 555 |
| MaeII | ACGT | 2 cut(s) | 477, 633 |
| MalI | GATC | 3 cut(s) | 423, 572, 708 |
| MboI | GATC | 3 cut(s) | 421, 570, 706 |
| MboII | GAAGA | 1 cut(s) | 848 |
| MhlI | GDGCHC | 1 cut(s) | 405 |
| MlsI | TGGCCA | 2 cut(s) | 189, 659 |
| MluCI | AATT | 5 cut(s) | 16, 127, 155, 228, 873 |
| MluNI | TGGCCA | 2 cut(s) | 189, 659 |
| MlyI | GAGTC | 1 cut(s) | 193 |
| Mox20I | TGGCCA | 2 cut(s) | 189, 659 |
| MscI | TGGCCA | 2 cut(s) | 189, 659 |
| MseI | TTAA | 3 cut(s) | 15, 416, 1085 |
| Msp20I | TGGCCA | 2 cut(s) | 189, 659 |
| MspI | CCGG | 2 cut(s) | 285, 565 |
| MspR9I | CCNGG | 2 cut(s) | 515, 565 |
| MvaI | CCWGG | 1 cut(s) | 515 |
| MvnI | CGCG | 1 cut(s) | 350 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 560, 785, 794, 894 |
| NciI | CCSGG | 1 cut(s) | 565 |
| NcoI | CCATGG | 3 cut(s) | 425, 549, 654 |
| NdeI | CATATG | 1 cut(s) | 687 |
| NdeII | GATC | 3 cut(s) | 421, 570, 706 |
| NheI | GCTAGC | 1 cut(s) | 554 |
| NlaIV | GGNNCC | 5 cut(s) | 107, 449, 512, 519, 652 |
| NspI | RCATGY | 1 cut(s) | 772 |
| PaeR7I | CTCGAG | 1 cut(s) | 574 |
| PagI | TCATGA | 1 cut(s) | 73 |
| PciI | ACATGT | 1 cut(s) | 768 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 265 |
| PfeI | GAWTC | 3 cut(s) | 270, 620, 947 |
| PkrI | GCNGC | 3 cut(s) | 151, 317, 790 |
| PleI | GAGTC | 1 cut(s) | 193 |
| PmaCI | CACGTG | 1 cut(s) | 478 |
| PmlI | CACGTG | 1 cut(s) | 478 |
| PpsI | GAGTC | 1 cut(s) | 193 |
| Ppu21I | YACGTR | 1 cut(s) | 478 |
| PpuMI | RGGWCCY | 1 cut(s) | 517 |
| PscI | ACATGT | 1 cut(s) | 768 |
| Psp5II | RGGWCCY | 1 cut(s) | 517 |
| Psp6I | CCWGG | 1 cut(s) | 513 |
| PspCI | CACGTG | 1 cut(s) | 478 |
| PspFI | CCCAGC | 1 cut(s) | 520 |
| PspGI | CCWGG | 1 cut(s) | 513 |
| PspN4I | GGNNCC | 5 cut(s) | 107, 449, 512, 519, 652 |
| PspPI | GGNCC | 2 cut(s) | 384, 517 |
| PspPPI | RGGWCCY | 1 cut(s) | 517 |
| RsaI | GTAC | 3 cut(s) | 333, 596, 702 |
| RsaNI | GTAC | 3 cut(s) | 332, 595, 701 |
| SaqAI | TTAA | 3 cut(s) | 15, 416, 1085 |
| SatI | GCNGC | 3 cut(s) | 150, 316, 789 |
| Sau3AI | GATC | 3 cut(s) | 421, 570, 706 |
| Sau96I | GGNCC | 2 cut(s) | 384, 517 |
| SchI | GAGTC | 1 cut(s) | 193 |
| ScrFI | CCNGG | 2 cut(s) | 515, 565 |
| SduI | GDGCHC | 1 cut(s) | 405 |
| SfaNI | GCATC | 4 cut(s) | 15, 550, 664, 784 |
| SfcI | CTRYAG | 1 cut(s) | 996 |
| Sfr274I | CTCGAG | 1 cut(s) | 574 |
| SinI | GGWCC | 1 cut(s) | 517 |
| SlaI | CTCGAG | 1 cut(s) | 574 |
| SmlI | CTYRAG | 3 cut(s) | 31, 574, 854 |
| SmoI | CTYRAG | 3 cut(s) | 31, 574, 854 |
| Sse9I | AATT | 5 cut(s) | 16, 127, 155, 228, 873 |
| SsiI | CCGC | 4 cut(s) | 315, 348, 639, 788 |
| SspMI | CTAG | 2 cut(s) | 297, 555 |
| StyD4I | CCNGG | 2 cut(s) | 513, 563 |
| StyI | CCWWGG | 3 cut(s) | 425, 549, 654 |
| TaaI | ACNGT | 2 cut(s) | 700, 922 |
| TaiI | ACGT | 2 cut(s) | 480, 636 |
| TaqI | TCGA | 1 cut(s) | 575 |
| TasI | AATT | 5 cut(s) | 16, 127, 155, 228, 873 |
| TatI | WGTACW | 3 cut(s) | 331, 594, 700 |
| TauI | GCSGC | 2 cut(s) | 318, 791 |
| TfiI | GAWTC | 3 cut(s) | 270, 620, 947 |
| Tru1I | TTAA | 3 cut(s) | 15, 416, 1085 |
| Tru9I | TTAA | 3 cut(s) | 15, 416, 1085 |
| TscAI | CASTG | 1 cut(s) | 703 |
| TseI | GCWGC | 1 cut(s) | 149 |
| TspDTI | ATGAA | 5 cut(s) | 112, 241, 704, 849, 1035 |
| TspRI | CASTG | 1 cut(s) | 703 |
| VpaK11BI | GGWCC | 1 cut(s) | 517 |
| XapI | RAATTY | 1 cut(s) | 127 |
| XceI | RCATGY | 1 cut(s) | 772 |
| XhoI | CTCGAG | 1 cut(s) | 574 |
| XspI | CTAG | 2 cut(s) | 297, 555 |