Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 992 |
| AciI | CCGC | 5 cut(s) | 171, 303, 594, 743, 746 |
| AclWI | GGATC | 1 cut(s) | 371 |
| AcoI | YGGCCR | 2 cut(s) | 139, 612 |
| AcsI | RAATTY | 1 cut(s) | 79 |
| AcyI | GRCGYC | 2 cut(s) | 297, 501 |
| AdeI | CACNNNGTG | 1 cut(s) | 436 |
| AfaI | GTAC | 1 cut(s) | 288 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 54, 381, 1034 |
| AflIII | ACRYGT | 1 cut(s) | 723 |
| AgsI | TTSAA | 5 cut(s) | 107, 496, 553, 566, 763 |
| AjiI | CACGTC | 1 cut(s) | 589 |
| AjnI | CCWGG | 1 cut(s) | 468 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 135, 167 |
| AluBI | AGCT | 6 cut(s) | 31, 72, 163, 197, 534, 988 |
| AluI | AGCT | 6 cut(s) | 31, 72, 163, 197, 534, 988 |
| Alw21I | GWGCWC | 1 cut(s) | 335 |
| Alw26I | GTCTC | 2 cut(s) | 172, 1050 |
| AlwI | GGATC | 1 cut(s) | 371 |
| Ama87I | CYCGRG | 1 cut(s) | 529 |
| AoxI | GGCC | 3 cut(s) | 139, 338, 612 |
| ApeKI | GCWGC | 1 cut(s) | 270 |
| ApoI | RAATTY | 1 cut(s) | 79 |
| AspS9I | GGNCC | 2 cut(s) | 339, 472 |
| AsuC2I | CCSGG | 1 cut(s) | 520 |
| AsuHPI | GGTGA | 1 cut(s) | 868 |
| AsuNHI | GCTAGC | 1 cut(s) | 509 |
| AvaI | CYCGRG | 1 cut(s) | 529 |
| AvaII | GGWCC | 1 cut(s) | 472 |
| BalI | TGGCCA | 2 cut(s) | 141, 614 |
| Bbv12I | GWGCWC | 1 cut(s) | 335 |
| BbvI | GCAGC | 1 cut(s) | 282 |
| BccI | CCATC | 2 cut(s) | 374, 934 |
| BceAI | ACGGC | 1 cut(s) | 409 |
| BciT130I | CCWGG | 1 cut(s) | 470 |
| BcnI | CCSGG | 1 cut(s) | 520 |
| BcoDI | GTCTC | 2 cut(s) | 172, 1050 |
| BfaI | CTAG | 1 cut(s) | 510 |
| BfuAI | ACCTGC | 1 cut(s) | 992 |
| BisI | GCNGC | 2 cut(s) | 271, 744 |
| BlpI | GCTNAGC | 1 cut(s) | 984 |
| BlsI | GCNGC | 2 cut(s) | 272, 745 |
| Bme1390I | CCNGG | 2 cut(s) | 470, 520 |
| Bme18I | GGWCC | 1 cut(s) | 472 |
| BmeT110I | CYCGRG | 1 cut(s) | 529 |
| BmgBI | CACGTC | 1 cut(s) | 589 |
| BmgT120I | GGNCC | 2 cut(s) | 339, 472 |
| BmiI | GGNNCC | 4 cut(s) | 59, 404, 474, 607 |
| BmrFI | CCNGG | 2 cut(s) | 470, 520 |
| BmsI | GCATC | 2 cut(s) | 505, 739 |
| BmtI | GCTAGC | 1 cut(s) | 513 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 152, 184 |
| BpmI | CTGGAG | 1 cut(s) | 802 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 984 |
| BpuEI | CTTGAG | 1 cut(s) | 830 |
| BpuMI | CCSGG | 1 cut(s) | 520 |
| BsaAI | YACGTR | 1 cut(s) | 433 |
| BsaHI | GRCGYC | 2 cut(s) | 297, 501 |
| BsaI | GGTCTC | 1 cut(s) | 1050 |
| BsaJI | CCNNGG | 3 cut(s) | 380, 504, 609 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 54, 381, 1034 |
| Bse1I | ACTGG | 1 cut(s) | 785 |
| Bse3DI | GCAATG | 1 cut(s) | 621 |
| BseBI | CCWGG | 1 cut(s) | 470 |
| BseDI | CCNNGG | 3 cut(s) | 380, 504, 609 |
| BseGI | GGATG | 4 cut(s) | 126, 204, 450, 754 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 54, 381, 1034 |
| BseMI | GCAATG | 1 cut(s) | 621 |
| BseMII | CTCAG | 1 cut(s) | 251 |
| BseNI | ACTGG | 1 cut(s) | 785 |
| BseRI | GAGGAG | 1 cut(s) | 299 |
| BseXI | GCAGC | 1 cut(s) | 282 |
| BsgI | GTGCAG | 1 cut(s) | 120 |
| BshFI | GGCC | 3 cut(s) | 141, 340, 614 |
| BsiHKAI | GWGCWC | 1 cut(s) | 335 |
| BsiHKCI | CYCGRG | 1 cut(s) | 529 |
| BsiSI | CCGG | 2 cut(s) | 237, 520 |
| BslFI | GGGAC | 3 cut(s) | 73, 613, 734 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 54, 381, 1034 |
| BsmAI | GTCTC | 2 cut(s) | 172, 1050 |
| BsmFI | GGGAC | 3 cut(s) | 73, 613, 734 |
| BsmI | GAATGC | 1 cut(s) | 800 |
| BsnI | GGCC | 3 cut(s) | 141, 340, 614 |
| Bso31I | GGTCTC | 1 cut(s) | 1050 |
| BsoBI | CYCGRG | 1 cut(s) | 529 |
| Bsp1286I | GDGCHC | 1 cut(s) | 335 |
| Bsp143I | GATC | 3 cut(s) | 376, 525, 661 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 984 |
| Bsp19I | CCATGG | 2 cut(s) | 380, 504 |
| BspACI | CCGC | 5 cut(s) | 171, 303, 594, 743, 746 |
| BspANI | GGCC | 3 cut(s) | 141, 340, 614 |
| BspCNI | CTCAG | 1 cut(s) | 252 |
| BspHI | TCATGA | 1 cut(s) | 25 |
| BspLI | GGNNCC | 4 cut(s) | 59, 404, 474, 607 |
| BspMI | ACCTGC | 1 cut(s) | 992 |
| BspOI | GCTAGC | 1 cut(s) | 513 |
| BspPI | GGATC | 1 cut(s) | 371 |
| BspTNI | GGTCTC | 1 cut(s) | 1050 |
| BsrDI | GCAATG | 1 cut(s) | 621 |
| BsrI | ACTGG | 1 cut(s) | 785 |
| BssECI | CCNNGG | 3 cut(s) | 380, 504, 609 |
| BssMI | GATC | 3 cut(s) | 376, 525, 661 |
| BssNI | GRCGYC | 2 cut(s) | 297, 501 |
| BssT1I | CCWWGG | 2 cut(s) | 380, 504 |
| Bst2UI | CCWGG | 1 cut(s) | 470 |
| Bst4CI | ACNGT | 3 cut(s) | 610, 655, 877 |
| BstACI | GRCGYC | 2 cut(s) | 297, 501 |
| BstBAI | YACGTR | 1 cut(s) | 433 |
| BstC8I | GCNNGC | 1 cut(s) | 511 |
| BstDEI | CTNAG | 3 cut(s) | 226, 260, 984 |
| BstDSI | CCRYGG | 3 cut(s) | 380, 504, 609 |
| BstF5I | GGATG | 4 cut(s) | 126, 204, 450, 754 |
| BstKTI | GATC | 3 cut(s) | 379, 528, 664 |
| BstMAI | GTCTC | 2 cut(s) | 172, 1050 |
| BstMBI | GATC | 3 cut(s) | 376, 525, 661 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 515, 740, 749 |
| BstNI | CCWGG | 1 cut(s) | 470 |
| BstNSI | RCATGY | 1 cut(s) | 727 |
| BstSCI | CCNGG | 2 cut(s) | 468, 518 |
| BstV1I | GCAGC | 1 cut(s) | 282 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 137 |
| BsuRI | GGCC | 3 cut(s) | 141, 340, 614 |
| BtgI | CCRYGG | 3 cut(s) | 380, 504, 609 |
| BtgZI | GCGATG | 2 cut(s) | 528, 829 |
| BtrI | CACGTC | 1 cut(s) | 589 |
| BtsCI | GGATG | 4 cut(s) | 126, 204, 450, 754 |
| BtsIMutI | CAGTG | 1 cut(s) | 651 |
| BveI | ACCTGC | 1 cut(s) | 992 |
| Cac8I | GCNNGC | 1 cut(s) | 511 |
| CciI | TCATGA | 1 cut(s) | 25 |
| Cfr13I | GGNCC | 2 cut(s) | 339, 472 |
| CseI | GACGC | 3 cut(s) | 305, 509, 1007 |
| Csp6I | GTAC | 1 cut(s) | 287 |
| CviQI | GTAC | 1 cut(s) | 287 |
| DdeI | CTNAG | 3 cut(s) | 226, 260, 984 |
| DpnI | GATC | 3 cut(s) | 378, 527, 663 |
| DpnII | GATC | 3 cut(s) | 376, 525, 661 |
| DraIII | CACNNNGTG | 1 cut(s) | 436 |
| EaeI | YGGCCR | 2 cut(s) | 139, 612 |
| EciI | GGCGGA | 2 cut(s) | 160, 761 |
| Eco130I | CCWWGG | 2 cut(s) | 380, 504 |
| Eco31I | GGTCTC | 1 cut(s) | 1050 |
| Eco32I | GATATC | 1 cut(s) | 115 |
| Eco47I | GGWCC | 1 cut(s) | 472 |
| Eco88I | CYCGRG | 1 cut(s) | 529 |
| EcoO109I | RGGNCCY | 2 cut(s) | 339, 472 |
| EcoRII | CCWGG | 1 cut(s) | 468 |
| EcoRV | GATATC | 1 cut(s) | 115 |
| EcoT14I | CCWWGG | 2 cut(s) | 380, 504 |
| ErhI | CCWWGG | 2 cut(s) | 380, 504 |
| FaqI | GGGAC | 3 cut(s) | 73, 613, 734 |
| FauNDI | CATATG | 1 cut(s) | 642 |
| Fnu4HI | GCNGC | 2 cut(s) | 271, 744 |
| FokI | GGATG | 4 cut(s) | 113, 191, 457, 761 |
| Fsp4HI | GCNGC | 2 cut(s) | 271, 744 |
| FspBI | CTAG | 1 cut(s) | 510 |
| GluI | GCNGC | 2 cut(s) | 271, 744 |
| GsuI | CTGGAG | 1 cut(s) | 802 |
| HaeIII | GGCC | 3 cut(s) | 141, 340, 614 |
| HapII | CCGG | 2 cut(s) | 237, 520 |
| HgaI | GACGC | 3 cut(s) | 305, 509, 1007 |
| Hin1I | GRCGYC | 2 cut(s) | 297, 501 |
| HincII | GTYRAC | 1 cut(s) | 586 |
| HindII | GTYRAC | 1 cut(s) | 586 |
| HinfI | GANTC | 5 cut(s) | 151, 222, 478, 575, 902 |
| HpaII | CCGG | 2 cut(s) | 237, 520 |
| HphI | GGTGA | 1 cut(s) | 868 |
| Hpy166II | GTNNAC | 2 cut(s) | 232, 586 |
| Hpy188I | TCNGA | 3 cut(s) | 160, 261, 997 |
| Hpy188III | TCNNGA | 3 cut(s) | 26, 313, 529 |
| Hpy8I | GTNNAC | 2 cut(s) | 232, 586 |
| HpyAV | CCTTC | 1 cut(s) | 352 |
| HpyCH4III | ACNGT | 3 cut(s) | 610, 655, 877 |
| HpyCH4IV | ACGT | 2 cut(s) | 432, 588 |
| HpyCH4V | TGCA | 6 cut(s) | 101, 439, 485, 651, 716, 775 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 515, 740, 749 |
| HpyF3I | CTNAG | 3 cut(s) | 226, 260, 984 |
| HpySE526I | ACGT | 2 cut(s) | 432, 588 |
| Hsp92I | GRCGYC | 2 cut(s) | 297, 501 |
| Kzo9I | GATC | 3 cut(s) | 376, 525, 661 |
| Lsp1109I | GCAGC | 1 cut(s) | 282 |
| LweI | GCATC | 2 cut(s) | 505, 739 |
| MaeI | CTAG | 1 cut(s) | 510 |
| MaeII | ACGT | 2 cut(s) | 432, 588 |
| MalI | GATC | 3 cut(s) | 378, 527, 663 |
| MboI | GATC | 3 cut(s) | 376, 525, 661 |
| MboII | GAAGA | 2 cut(s) | 803, 916 |
| MhlI | GDGCHC | 1 cut(s) | 335 |
| MlsI | TGGCCA | 2 cut(s) | 141, 614 |
| MluCI | AATT | 4 cut(s) | 79, 107, 180, 828 |
| MluNI | TGGCCA | 2 cut(s) | 141, 614 |
| MlyI | GAGTC | 1 cut(s) | 472 |
| MnlI | CCTC | 9 cut(s) | 101, 224, 310, 320, 648, 678, 724, 832, 1048 |
| Mox20I | TGGCCA | 2 cut(s) | 141, 614 |
| MscI | TGGCCA | 2 cut(s) | 141, 614 |
| MseI | TTAA | 3 cut(s) | 371, 951, 1040 |
| Msp20I | TGGCCA | 2 cut(s) | 141, 614 |
| MspI | CCGG | 2 cut(s) | 237, 520 |
| MspR9I | CCNGG | 2 cut(s) | 470, 520 |
| Mva1269I | GAATGC | 1 cut(s) | 800 |
| MvaI | CCWGG | 1 cut(s) | 470 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 515, 740, 749 |
| NciI | CCSGG | 1 cut(s) | 520 |
| NcoI | CCATGG | 2 cut(s) | 380, 504 |
| NdeI | CATATG | 1 cut(s) | 642 |
| NdeII | GATC | 3 cut(s) | 376, 525, 661 |
| NheI | GCTAGC | 1 cut(s) | 509 |
| NlaIV | GGNNCC | 4 cut(s) | 59, 404, 474, 607 |
| NspI | RCATGY | 1 cut(s) | 727 |
| PaeR7I | CTCGAG | 1 cut(s) | 529 |
| PagI | TCATGA | 1 cut(s) | 25 |
| PciI | ACATGT | 1 cut(s) | 723 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 217 |
| PctI | GAATGC | 1 cut(s) | 800 |
| PfeI | GAWTC | 4 cut(s) | 151, 222, 575, 902 |
| PkrI | GCNGC | 2 cut(s) | 272, 745 |
| PleI | GAGTC | 1 cut(s) | 472 |
| PpsI | GAGTC | 1 cut(s) | 472 |
| Ppu21I | YACGTR | 1 cut(s) | 433 |
| PpuMI | RGGWCCY | 1 cut(s) | 472 |
| PscI | ACATGT | 1 cut(s) | 723 |
| Psp5II | RGGWCCY | 1 cut(s) | 472 |
| Psp6I | CCWGG | 1 cut(s) | 468 |
| PspGI | CCWGG | 1 cut(s) | 468 |
| PspN4I | GGNNCC | 4 cut(s) | 59, 404, 474, 607 |
| PspPI | GGNCC | 2 cut(s) | 339, 472 |
| PspPPI | RGGWCCY | 1 cut(s) | 472 |
| RsaI | GTAC | 1 cut(s) | 288 |
| RsaNI | GTAC | 1 cut(s) | 287 |
| SaqAI | TTAA | 3 cut(s) | 371, 951, 1040 |
| SatI | GCNGC | 2 cut(s) | 271, 744 |
| Sau3AI | GATC | 3 cut(s) | 376, 525, 661 |
| Sau96I | GGNCC | 2 cut(s) | 339, 472 |
| SchI | GAGTC | 1 cut(s) | 472 |
| ScrFI | CCNGG | 2 cut(s) | 470, 520 |
| SduI | GDGCHC | 1 cut(s) | 335 |
| SfaNI | GCATC | 2 cut(s) | 505, 739 |
| Sfr274I | CTCGAG | 1 cut(s) | 529 |
| SinI | GGWCC | 1 cut(s) | 472 |
| SlaI | CTCGAG | 1 cut(s) | 529 |
| SmlI | CTYRAG | 2 cut(s) | 529, 809 |
| SmoI | CTYRAG | 2 cut(s) | 529, 809 |
| Sse9I | AATT | 4 cut(s) | 79, 107, 180, 828 |
| SsiI | CCGC | 5 cut(s) | 171, 303, 594, 743, 746 |
| SspMI | CTAG | 1 cut(s) | 510 |
| StyD4I | CCNGG | 2 cut(s) | 468, 518 |
| StyI | CCWWGG | 2 cut(s) | 380, 504 |
| TaaI | ACNGT | 3 cut(s) | 610, 655, 877 |
| TaiI | ACGT | 2 cut(s) | 435, 591 |
| TaqI | TCGA | 2 cut(s) | 530, 927 |
| TasI | AATT | 4 cut(s) | 79, 107, 180, 828 |
| TatI | WGTACW | 1 cut(s) | 286 |
| TauI | GCSGC | 1 cut(s) | 746 |
| TfiI | GAWTC | 4 cut(s) | 151, 222, 575, 902 |
| Tru1I | TTAA | 3 cut(s) | 371, 951, 1040 |
| Tru9I | TTAA | 3 cut(s) | 371, 951, 1040 |
| TscAI | CASTG | 1 cut(s) | 658 |
| TseI | GCWGC | 1 cut(s) | 270 |
| TspDTI | ATGAA | 4 cut(s) | 64, 193, 659, 804 |
| TspRI | CASTG | 1 cut(s) | 658 |
| VpaK11BI | GGWCC | 1 cut(s) | 472 |
| XapI | RAATTY | 1 cut(s) | 79 |
| XceI | RCATGY | 1 cut(s) | 727 |
| XhoI | CTCGAG | 1 cut(s) | 529 |
| XspI | CTAG | 1 cut(s) | 510 |