Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0000443

Physical Location & Seq

Genomic Coordinates Reverse (-)

28049106 .. 28051283

Gene Structure 2178 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g35550.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGGCGAGCCTTAGCTACCTTCTTTATGTTTTGGTAAGCCTTTTGAACCCAGTTAGCTACCTTCTTTATGGTCATGACCGGTTCAACTATAAACCAGCAGCAGACGATAATGAGGTACAATATAAGACCTTGTTTGTGTTTGGAGACTCGCTGTATGATCCTGGCAACGATCAATACCTCCCTAATGTCACCGTAGAAGAATACCCAGCAACAACCTGGCCATATGGAGAAACCTTCTTTCATCGCCCCAACGGAAGACTTTCCGATGGCCGTATAGTCCCGGACTTCATCGCCAAGTTAGCAAACTTGCCAATGCCTCCACCCTACCTGCAACCAGATCCAAAAAATTATACCAACGGAGTCAGCTTTGCTTCAGCCGGTGCCGGAGTTCTAGTTGAAACTGATCCAGGAACTATAAATCTTCCATTACAATTGAGCTACTTTAAGAACGTGACGGAGTTGTTGCAAGAGCAACTCGGTGATGAAGAGGCCAAGAAGTTGCTGGGGAGTGCTGTGTACTTGATTAGCATGGGAGGCAATGACTATTTTACCTTCTACGCTGAATACCAAGAAAATGCCACCGAGGCCCTCCAGCAGGAATTCATAGGAATTGTTATCGGCAACTTGACCACTGTTCTCCAAGGAATATACGACTTAGGTGGGAGGAAAATAGCATTTCAAAATGCAGGACCTCTTGGGTGCTTACCTTACGCAAAACAAGAATACGACACTCAACTTGTAATTGGGTGCGTTGAAGGGCTCCAGAGTCTGGCAAGGCAACACAATAAAGCTCTAGCTAGTGTCCTCAAAGAGCTAGAGAGCCAACTACCGGGGTTCAAATATTCGATATTCGAGTACTATGATGCACTTGGCGACCGAGTTCTTAACCCCAGCAAATACGGCTTTAAGGATGGAACAGATGCGTGTTGCGGTAGAGGGGCGTACAGAGGATCTGATTGTGGCGGACTAAATGGAACGGTGGCATATGAGTTATGTAGTAATCCTGGAGATAGTGTGTGGTTTGATGGGAGTCATACAACTGAAAGGGCAAACCTGCAGTTGGCGGAACTGATTTGGAGTGGAACACCCAATGTGACATGGCCTTACAATGTCCAACAACTTTTTGAACAAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005840 GO:0005911 GO:0006082 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009409 GO:0009505 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009941 GO:0009987 GO:0016020 GO:0016143 GO:0016145 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0022626 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032991 GO:0042579 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0044237 GO:0044248 GO:0044273 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_GDSL

Protein Analysis

379

Amino Acids

41.88

Weight (kDa)

4.54

Isoelectric Point (pI)

33.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_GDSL	PF00657	44 - 358	3.8e-31	GDSL-like Lipase/Acylhydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000443)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35490 FvH4_6g35490 FvH4_6g35500 FvH4_6g35500 FvH4_6g35500 FvH4_6g35520 FvH4_6g35520 FvH4_6g35540 FvH4_6g35550
malus_domestica	MD09G1175400.v1.1
pyrus_communis	pycom09g09260
rosa_chinensis	RchiOBHm_Chr2g0144821 RchiOBHm_Chr2g0145011 RchiOBHm_Chr2g0145061 RchiOBHm_Chr2g0147051 RchiOBHm_Chr2g0147071 RchiOBHm_Chr2g0147111 RchiOBHm_Chr2g0147141
rosa_laevigata	RLG00000020116 RLG00000020119 RLG00000020120 RLG00000020122 RLG00000020243 RLG00000020245 RLG00000020246
rosa_multiflora	Rmu_co8407447.1_g000001 Rmu_sc0000070.1_g000059 Rmu_sc0001668.1_g000005 Rmu_sc0001880.1_g000013 Rmu_sc0001880.1_g000020 Rmu_sc0001880.1_g000023 Rmu_sc0001880.1_g000027 Rmu_sc0002354.1_g000023 Rmu_sc0002506.1_g000001 Rmu_sc0002506.1_g000016 Rmu_sc0004897.1_g000008 Rmu_sc0007988.1_g000001
rosa_roxburghii	Rroxscaffold_1G00033230 Rroxscaffold_2G00099670 Rroxscaffold_2G00099720 Rroxscaffold_2G00100900 Rroxscaffold_2G00100930 Rroxscaffold_2G00100940
rosa_rugosa	Rorug02G0390000 Rorug02G0390100 Rorug02G0390200 Rorug02G0390200 Rorug02G0390200 Rorug02G0398400 Rorug02G0398500 Rorug02G0398600
rosa_samantha	Rh2AG442200 Rh2AG442500 Rh2AG442700 Rh2AG455600 Rh2AG455700 Rh2AG455800 Rh2AG455900 Rh2BG452500 Rh2BG453000 Rh2BG453700 Rh2BG467900 Rh2BG468100 Rh2BG468200 Rh2CG429200 Rh2CG429500 Rh2CG429600 Rh2CG429700 Rh2CG443000 Rh2CG443100 Rh2CG443200 Rh2CG443300 Rh2DG462200 Rh2DG463000 Rh2DG463200 Rh2DG463700 Rh2DG477300 Rh2DG477500 Rh2DG477600 Rh4AG390700 Rh5AG320200
rosa_wichuraiana	Rw2G036130 Rw2G036170 Rw2G036180 Rw2G037150 Rw2G037160 Rw2G037170 Rw2G037180 Rw2G037260 Rw2G037270 Rw2G037280 Rw2G037290 Rw5G030070 Rw5G030220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	336, 1062
AccB1I	GGYRCC	1 cut(s)	380
AccB7I	CCANNNNNTGG	1 cut(s)	769
AciI	CCGC	3 cut(s)	930, 963, 1064
AclWI	GGATC	4 cut(s)	152, 332, 398, 958
AcoI	YGGCCR	2 cut(s)	218, 268
AcsI	RAATTY	1 cut(s)	599
AcuI	CTGAAG	1 cut(s)	357
AfaI	GTAC	4 cut(s)	117, 518, 857, 944
AfiI	CCNNNNNNNGG	4 cut(s)	595, 769, 829, 1060
AgeI	ACCGGT	1 cut(s)	78
AgsI	TTSAA	7 cut(s)	46, 85, 398, 680, 755, 838, 1127
AjnI	CCWGG	4 cut(s)	160, 215, 406, 1003
AluBI	AGCT	7 cut(s)	15, 57, 366, 438, 791, 797, 814
AluI	AGCT	7 cut(s)	15, 57, 366, 438, 791, 797, 814
Alw26I	GTCTC	1 cut(s)	138
AlwI	GGATC	4 cut(s)	152, 332, 398, 958
AlwNI	CAGNNNCTG	1 cut(s)	769
AoxI	GGCC	5 cut(s)	218, 268, 489, 585, 1100
ApeKI	GCWGC	1 cut(s)	98
ApoI	RAATTY	1 cut(s)	599
AsiGI	ACCGGT	1 cut(s)	78
Asp700I	GAANNNNTTC	1 cut(s)	259
AspS9I	GGNCC	2 cut(s)	586, 689
AsuC2I	CCSGG	2 cut(s)	281, 831
AsuHPI	GGTGA	2 cut(s)	181, 491
AvaII	GGWCC	1 cut(s)	689
BalI	TGGCCA	1 cut(s)	220
BanI	GGYRCC	1 cut(s)	380
BanII	GRGCYC	1 cut(s)	762
BbsI	GAAGAC	1 cut(s)	262
BbvI	GCAGC	1 cut(s)	110
BccI	CCATC	3 cut(s)	260, 905, 1019
BceAI	ACGGC	2 cut(s)	255, 916
BciT130I	CCWGG	4 cut(s)	162, 217, 408, 1005
BcnI	CCSGG	2 cut(s)	281, 831
BcoDI	GTCTC	1 cut(s)	138
BfaI	CTAG	4 cut(s)	392, 794, 798, 815
BfmI	CTRYAG	1 cut(s)	1055
BfuAI	ACCTGC	2 cut(s)	336, 1062
BglI	GCCNNNNNGGC	1 cut(s)	584
BisI	GCNGC	1 cut(s)	99
BlsI	GCNGC	1 cut(s)	100
BmcAI	AGTACT	1 cut(s)	857
Bme1390I	CCNGG	6 cut(s)	162, 217, 281, 408, 831, 1005
Bme18I	GGWCC	1 cut(s)	689
BmgT120I	GGNCC	2 cut(s)	586, 689
BmiI	GGNNCC	2 cut(s)	382, 761
BmrFI	CCNGG	6 cut(s)	162, 217, 281, 408, 831, 1005
BmrI	ACTGGG	1 cut(s)	44
BmsI	GCATC	2 cut(s)	853, 910
BmuI	ACTGGG	1 cut(s)	44
BpiI	GAAGAC	1 cut(s)	262
BpmI	CTGGAG	3 cut(s)	575, 746, 1026
Bpu10I	CCTNAGC	1 cut(s)	11
BpuMI	CCSGG	2 cut(s)	281, 831
BsaJI	CCNNGG	3 cut(s)	582, 640, 830
BsaWI	WCCGGW	1 cut(s)	78
BsaXI	ACNNNNNCTCC	4 cut(s)	378, 408, 999, 1029
Bsc4I	CCNNNNNNNGG	4 cut(s)	595, 769, 829, 1060
Bse118I	RCCGGY	2 cut(s)	78, 377
Bse1I	ACTGG	1 cut(s)	50
Bse3DI	GCAATG	1 cut(s)	544
BseBI	CCWGG	4 cut(s)	162, 217, 408, 1005
BseDI	CCNNGG	3 cut(s)	582, 640, 830
BseGI	GGATG	1 cut(s)	916
BseLI	CCNNNNNNNGG	4 cut(s)	595, 769, 829, 1060
BseMI	GCAATG	1 cut(s)	544
BseNI	ACTGG	1 cut(s)	50
BseXI	GCAGC	1 cut(s)	110
BseYI	CCCAGC	3 cut(s)	205, 502, 890
Bsh1285I	CGRYCG	1 cut(s)	877
BshFI	GGCC	5 cut(s)	220, 270, 491, 587, 1102
BshNI	GGYRCC	1 cut(s)	380
BshTI	ACCGGT	1 cut(s)	78
BsiEI	CGRYCG	1 cut(s)	877
BsiSI	CCGG	5 cut(s)	79, 281, 378, 384, 830
BslFI	GGGAC	1 cut(s)	263
BslI	CCNNNNNNNGG	4 cut(s)	595, 769, 829, 1060
BsmAI	GTCTC	1 cut(s)	138
BsmFI	GGGAC	1 cut(s)	263
BsnI	GGCC	5 cut(s)	220, 270, 491, 587, 1102
Bsp1286I	GDGCHC	1 cut(s)	762
Bsp143I	GATC	5 cut(s)	157, 169, 337, 403, 950
BspACI	CCGC	3 cut(s)	930, 963, 1064
BspANI	GGCC	5 cut(s)	220, 270, 491, 587, 1102
BspHI	TCATGA	1 cut(s)	73
BspLI	GGNNCC	2 cut(s)	382, 761
BspMAI	CTGCAG	1 cut(s)	1059
BspMI	ACCTGC	2 cut(s)	336, 1062
BspPI	GGATC	4 cut(s)	152, 332, 398, 958
BspT107I	GGYRCC	1 cut(s)	380
BsrDI	GCAATG	1 cut(s)	544
BsrFI	RCCGGY	2 cut(s)	78, 377
BsrI	ACTGG	1 cut(s)	50
BssAI	RCCGGY	2 cut(s)	78, 377
BssECI	CCNNGG	3 cut(s)	582, 640, 830
BssMI	GATC	5 cut(s)	157, 169, 337, 403, 950
BssT1I	CCWWGG	1 cut(s)	640
Bst2UI	CCWGG	4 cut(s)	162, 217, 408, 1005
Bst4CI	ACNGT	3 cut(s)	193, 634, 979
Bst6I	CTCTTC	1 cut(s)	480
BstC8I	GCNNGC	1 cut(s)	7
BstDEI	CTNAG	2 cut(s)	11, 655
BstENI	CCTNNNNNAGG	1 cut(s)	593
BstF5I	GGATG	1 cut(s)	916
BstKTI	GATC	5 cut(s)	160, 172, 340, 406, 953
BstMAI	GTCTC	1 cut(s)	138
BstMBI	GATC	5 cut(s)	157, 169, 337, 403, 950
BstMCI	CGRYCG	1 cut(s)	877
BstMWI	GCNNNNNNNGC	4 cut(s)	299, 534, 584, 900
BstNI	CCWGG	4 cut(s)	162, 217, 408, 1005
BstSCI	CCNGG	6 cut(s)	160, 215, 279, 406, 829, 1003
BstSFI	CTRYAG	1 cut(s)	1055
BstV1I	GCAGC	1 cut(s)	110
BstV2I	GAAGAC	1 cut(s)	262
BstX2I	RGATCY	2 cut(s)	337, 950
BstYI	RGATCY	2 cut(s)	337, 950
BsuRI	GGCC	5 cut(s)	220, 270, 491, 587, 1102
BtgZI	GCGATG	2 cut(s)	227, 274
BtsCI	GGATG	1 cut(s)	916
BtsIMutI	CAGTG	1 cut(s)	630
BveI	ACCTGC	2 cut(s)	336, 1062
Cac8I	GCNNGC	1 cut(s)	7
CaiI	CAGNNNCTG	1 cut(s)	769
CciI	TCATGA	1 cut(s)	73
Cfr10I	RCCGGY	2 cut(s)	78, 377
Cfr13I	GGNCC	2 cut(s)	586, 689
Csp6I	GTAC	4 cut(s)	116, 517, 856, 943
CspAI	ACCGGT	1 cut(s)	78
CviAII	CATG	3 cut(s)	74, 529, 1098
CviQI	GTAC	4 cut(s)	116, 517, 856, 943
DdeI	CTNAG	2 cut(s)	11, 655
DpnI	GATC	5 cut(s)	159, 171, 339, 405, 952
DpnII	GATC	5 cut(s)	157, 169, 337, 403, 950
EaeI	YGGCCR	2 cut(s)	218, 268
Eam1104I	CTCTTC	1 cut(s)	480
EarI	CTCTTC	1 cut(s)	480
EciI	GGCGGA	2 cut(s)	978, 1079
Eco130I	CCWWGG	1 cut(s)	640
Eco24I	GRGCYC	1 cut(s)	762
Eco47I	GGWCC	1 cut(s)	689
Eco57I	CTGAAG	1 cut(s)	357
EcoNI	CCTNNNNNAGG	1 cut(s)	593
EcoO109I	RGGNCCY	2 cut(s)	586, 689
EcoRI	GAATTC	1 cut(s)	599
EcoRII	CCWGG	4 cut(s)	160, 215, 406, 1003
EcoT14I	CCWWGG	1 cut(s)	640
EcoT38I	GRGCYC	1 cut(s)	762
ErhI	CCWWGG	1 cut(s)	640
FaeI	CATG	3 cut(s)	77, 532, 1101
FaqI	GGGAC	1 cut(s)	263
FatI	CATG	3 cut(s)	73, 528, 1097
FauNDI	CATATG	2 cut(s)	223, 985
Fnu4HI	GCNGC	1 cut(s)	99
FokI	GGATG	1 cut(s)	923
FriOI	GRGCYC	1 cut(s)	762
Fsp4HI	GCNGC	1 cut(s)	99
FspBI	CTAG	4 cut(s)	392, 794, 798, 815
GluI	GCNGC	1 cut(s)	99
GsaI	CCCAGC	3 cut(s)	209, 506, 894
GsuI	CTGGAG	3 cut(s)	575, 746, 1026
HaeIII	GGCC	5 cut(s)	220, 270, 491, 587, 1102
HapII	CCGG	5 cut(s)	79, 281, 378, 384, 830
Hin1II	CATG	3 cut(s)	77, 532, 1101
HinfI	GANTC	4 cut(s)	146, 360, 766, 1030
HpaII	CCGG	5 cut(s)	79, 281, 378, 384, 830
HphI	GGTGA	2 cut(s)	181, 491
Hpy166II	GTNNAC	1 cut(s)	517
Hpy188I	TCNGA	2 cut(s)	265, 955
Hpy188III	TCNNGA	2 cut(s)	74, 763
Hpy8I	GTNNAC	1 cut(s)	517
HpyAV	CCTTC	5 cut(s)	29, 71, 244, 562, 749
HpyCH4III	ACNGT	3 cut(s)	193, 634, 979
HpyCH4IV	ACGT	1 cut(s)	450
HpyCH4V	TGCA	5 cut(s)	331, 466, 686, 866, 1057
HpyF10VI	GCNNNNNNNGC	4 cut(s)	299, 534, 584, 900
HpyF3I	CTNAG	2 cut(s)	11, 655
HpySE526I	ACGT	1 cut(s)	450
Hsp92II	CATG	3 cut(s)	77, 532, 1101
Kzo9I	GATC	5 cut(s)	157, 169, 337, 403, 950
LmnI	GCTCC	1 cut(s)	765
Lsp1109I	GCAGC	1 cut(s)	110
LweI	GCATC	2 cut(s)	853, 910
MaeI	CTAG	4 cut(s)	392, 794, 798, 815
MaeII	ACGT	1 cut(s)	450
MaeIII	GTNAC	3 cut(s)	187, 451, 1093
MalI	GATC	5 cut(s)	159, 171, 339, 405, 952
MboI	GATC	5 cut(s)	157, 169, 337, 403, 950
MboII	GAAGA	4 cut(s)	209, 267, 413, 497
MfeI	CAATTG	1 cut(s)	431
MflI	RGATCY	2 cut(s)	337, 950
MhlI	GDGCHC	1 cut(s)	762
MlsI	TGGCCA	1 cut(s)	220
MluCI	AATT	5 cut(s)	346, 431, 599, 609, 741
MluNI	TGGCCA	1 cut(s)	220
MlyI	GAGTC	4 cut(s)	140, 369, 775, 1039
Mox20I	TGGCCA	1 cut(s)	220
MroXI	GAANNNNTTC	1 cut(s)	259
MscI	TGGCCA	1 cut(s)	220
MseI	TTAA	3 cut(s)	444, 885, 906
Msp20I	TGGCCA	1 cut(s)	220
MspI	CCGG	5 cut(s)	79, 281, 378, 384, 830
MspR9I	CCNGG	6 cut(s)	162, 217, 281, 408, 831, 1005
MunI	CAATTG	1 cut(s)	431
MvaI	CCWGG	4 cut(s)	162, 217, 408, 1005
MwoI	GCNNNNNNNGC	4 cut(s)	299, 534, 584, 900
NciI	CCSGG	2 cut(s)	281, 831
NdeI	CATATG	2 cut(s)	223, 985
NdeII	GATC	5 cut(s)	157, 169, 337, 403, 950
NlaIII	CATG	3 cut(s)	77, 532, 1101
NlaIV	GGNNCC	2 cut(s)	382, 761
NmuCI	GTSAC	3 cut(s)	187, 451, 1093
PagI	TCATGA	1 cut(s)	73
PdmI	GAANNNNTTC	1 cut(s)	259
PflMI	CCANNNNNTGG	1 cut(s)	769
PfoI	TCCNGGA	3 cut(s)	279, 406, 1003
PinAI	ACCGGT	1 cut(s)	78
PkrI	GCNGC	1 cut(s)	100
PleI	GAGTC	4 cut(s)	140, 368, 774, 1038
PpsI	GAGTC	4 cut(s)	140, 368, 774, 1038
PpuMI	RGGWCCY	1 cut(s)	689
Psp5II	RGGWCCY	1 cut(s)	689
Psp6I	CCWGG	4 cut(s)	160, 215, 406, 1003
PspFI	CCCAGC	3 cut(s)	205, 502, 890
PspGI	CCWGG	4 cut(s)	160, 215, 406, 1003
PspN4I	GGNNCC	2 cut(s)	382, 761
PspPI	GGNCC	2 cut(s)	586, 689
PspPPI	RGGWCCY	1 cut(s)	689
PstI	CTGCAG	1 cut(s)	1059
PstNI	CAGNNNCTG	1 cut(s)	769
PsuI	RGATCY	2 cut(s)	337, 950
RsaI	GTAC	4 cut(s)	117, 518, 857, 944
RsaNI	GTAC	4 cut(s)	116, 517, 856, 943
SaqAI	TTAA	3 cut(s)	444, 885, 906
SatI	GCNGC	1 cut(s)	99
Sau3AI	GATC	5 cut(s)	157, 169, 337, 403, 950
Sau96I	GGNCC	2 cut(s)	586, 689
ScaI	AGTACT	1 cut(s)	857
SchI	GAGTC	4 cut(s)	140, 369, 775, 1039
ScrFI	CCNGG	6 cut(s)	162, 217, 281, 408, 831, 1005
SduI	GDGCHC	1 cut(s)	762
SfaNI	GCATC	2 cut(s)	853, 910
SfcI	CTRYAG	1 cut(s)	1055
SinI	GGWCC	1 cut(s)	689
Sse9I	AATT	5 cut(s)	346, 431, 599, 609, 741
SsiI	CCGC	3 cut(s)	930, 963, 1064
SspI	AATATT	1 cut(s)	842
SspMI	CTAG	4 cut(s)	392, 794, 798, 815
StyD4I	CCNGG	6 cut(s)	160, 215, 279, 406, 829, 1003
StyI	CCWWGG	1 cut(s)	640
TaaI	ACNGT	3 cut(s)	193, 634, 979
TaiI	ACGT	1 cut(s)	453
TaqI	TCGA	2 cut(s)	845, 852
TaqII	GACCGA	1 cut(s)	891
TasI	AATT	5 cut(s)	346, 431, 599, 609, 741
TatI	WGTACW	2 cut(s)	516, 855
Tru1I	TTAA	3 cut(s)	444, 885, 906
Tru9I	TTAA	3 cut(s)	444, 885, 906
TscAI	CASTG	1 cut(s)	637
TseFI	GTSAC	3 cut(s)	187, 451, 1093
TseI	GCWGC	1 cut(s)	98
Tsp45I	GTSAC	3 cut(s)	187, 451, 1093
TspDTI	ATGAA	4 cut(s)	230, 277, 498, 592
TspGWI	ACGGA	3 cut(s)	267, 372, 470
TspRI	CASTG	1 cut(s)	637
Van91I	CCANNNNNTGG	1 cut(s)	769
VpaK11BI	GGWCC	1 cut(s)	689
XagI	CCTNNNNNAGG	1 cut(s)	593
XapI	RAATTY	1 cut(s)	599
XcmI	CCANNNNNNNNNTGG	2 cut(s)	213, 499
XmnI	GAANNNNTTC	1 cut(s)	259
XspI	CTAG	4 cut(s)	392, 794, 798, 815
ZrmI	AGTACT	1 cut(s)	857

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.