Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000443

Physical Location & Seq

Genomic Coordinates Reverse (-)

61333296 .. 61340737

Gene Structure 7442 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020122

Sequence Viewer

CDS Protein Promoter (2k)

Length: 960 bp

ATGGTGAGTCTTAGCTACATTTTTTACGTGTTGGCTAGCCTATTCAACCCAATTAGCTTCCTTCTCTACGGTCATCATCATGAGCATCCCGATCAGTACTCCAGTAGTACTAGTATAGAACACAATCATAATAAGACACTGATGCCCTCCCTCGCAGCCTTTATCCAACCGGCAACTGGGTCCCATGCCCAAATCGATTCCATAGTAGTCACTAGAGCACCATTGTTGCGAGCACAAACCACACCCATAAGTCTTCCGACGCAGTTAAGCTATTTAAAAAATGTGGCAAAGTTGCTGGAGCTTAAGTTAGGTGATTTAGAAGCCAAACGTGTGCTGAGGACCGCTGTTTACTTGTTCAGCGTCGGAGGGGTGGATGATATGGTCTTCTACTCTACATATCCAAATGCCACCGAGTCCCTCCAATCCGAATTCATAGCAAATGTGAATGGTAACTTGACCGATGTGCTCAAAGAAATATACAACTTAGGAGGAAGGAAGATTGCATTTCAGAATGTAGGATCTCTTGGGTGCCAACCTATAAATAGACAATCATACGGTGTCACTGAAGGGTGTGTTGAAGGACTTTTGACTCTGGCAAGGCTACACAATAGAGCTCTAGCTAATGCCCTCGAAGAGCTGGAGCTAAAGCTATCAGGATTCAAATATTCAATATTTAACTACTATGATGCGCTTTTGGACCTATACCATAACCCTGCAAAACATGGGTTCACAAATGGGTCGGATGCATGTTGTGGTATAGGACCATACCGAGGAGATGATTGCGGCGGAGATAATGGAACCGAACCATTTGAATTGTGCCCAAACCCAGGTGACAATGTGTGGTGGGATGGTGGCCATTCGACTGAAAGAGCAAACCTTAAATTGGCAGAGCTAATATGGAGTGGCACGCTAAATACTACCGGGCCTTACAATGTCAAACAGTTTTTTCAACAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005840 GO:0005911 GO:0006082 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009409 GO:0009505 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009941 GO:0009987 GO:0016020 GO:0016143 GO:0016145 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0022626 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032991 GO:0042579 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0044237 GO:0044248 GO:0044273 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_GDSL

Protein Analysis

320

Amino Acids

35.17

Weight (kDa)

5.74

Isoelectric Point (pI)

31.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_GDSL	PF00657	110 - 299	7.5e-12	GDSL-like Lipase/Acylhydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000443)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35490 FvH4_6g35490 FvH4_6g35500 FvH4_6g35500 FvH4_6g35500 FvH4_6g35520 FvH4_6g35520 FvH4_6g35540 FvH4_6g35550
malus_domestica	MD09G1175400.v1.1
pyrus_communis	pycom09g09260
rosa_chinensis	RchiOBHm_Chr2g0144821 RchiOBHm_Chr2g0145011 RchiOBHm_Chr2g0145061 RchiOBHm_Chr2g0147051 RchiOBHm_Chr2g0147071 RchiOBHm_Chr2g0147111 RchiOBHm_Chr2g0147141
rosa_laevigata	RLG00000020116 RLG00000020119 RLG00000020120 RLG00000020122 RLG00000020243 RLG00000020245 RLG00000020246
rosa_multiflora	Rmu_co8407447.1_g000001 Rmu_sc0000070.1_g000059 Rmu_sc0001668.1_g000005 Rmu_sc0001880.1_g000013 Rmu_sc0001880.1_g000020 Rmu_sc0001880.1_g000023 Rmu_sc0001880.1_g000027 Rmu_sc0002354.1_g000023 Rmu_sc0002506.1_g000001 Rmu_sc0002506.1_g000016 Rmu_sc0004897.1_g000008 Rmu_sc0007988.1_g000001
rosa_roxburghii	Rroxscaffold_1G00033230 Rroxscaffold_2G00099670 Rroxscaffold_2G00099720 Rroxscaffold_2G00100900 Rroxscaffold_2G00100930 Rroxscaffold_2G00100940
rosa_rugosa	Rorug02G0390000 Rorug02G0390100 Rorug02G0390200 Rorug02G0390200 Rorug02G0390200 Rorug02G0398400 Rorug02G0398500 Rorug02G0398600
rosa_samantha	Rh2AG442200 Rh2AG442500 Rh2AG442700 Rh2AG455600 Rh2AG455700 Rh2AG455800 Rh2AG455900 Rh2BG452500 Rh2BG453000 Rh2BG453700 Rh2BG467900 Rh2BG468100 Rh2BG468200 Rh2CG429200 Rh2CG429500 Rh2CG429600 Rh2CG429700 Rh2CG443000 Rh2CG443100 Rh2CG443200 Rh2CG443300 Rh2DG462200 Rh2DG463000 Rh2DG463200 Rh2DG463700 Rh2DG477300 Rh2DG477500 Rh2DG477600 Rh4AG390700 Rh5AG320200
rosa_wichuraiana	Rw2G036130 Rw2G036170 Rw2G036180 Rw2G037150 Rw2G037160 Rw2G037170 Rw2G037180 Rw2G037260 Rw2G037270 Rw2G037280 Rw2G037290 Rw5G030070 Rw5G030220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	528
AciI	CCGC	3 cut(s)	342, 783, 786
AclWI	GGATC	1 cut(s)	526
AcoI	YGGCCR	1 cut(s)	853
AcsI	RAATTY	1 cut(s)	428
AcuI	CTGAAG	1 cut(s)	585
AfaI	GTAC	2 cut(s)	98, 109
AfiI	CCNNNNNNNGG	4 cut(s)	176, 770, 827, 883
AflII	CTTAAG	1 cut(s)	302
AflIII	ACRYGT	2 cut(s)	27, 328
AgsI	TTSAA	6 cut(s)	46, 578, 661, 669, 812, 950
AhlI	ACTAGT	1 cut(s)	110
AjnI	CCWGG	1 cut(s)	826
Alw21I	GWGCWC	4 cut(s)	220, 235, 468, 616
AlwI	GGATC	1 cut(s)	526
AoxI	GGCC	2 cut(s)	853, 923
ApeKI	GCWGC	1 cut(s)	155
ApoI	RAATTY	1 cut(s)	428
AspLEI	GCGC	1 cut(s)	691
AspS9I	GGNCC	5 cut(s)	180, 339, 697, 761, 923
AsuC2I	CCSGG	1 cut(s)	922
AsuHPI	GGTGA	3 cut(s)	16, 323, 842
AsuNHI	GCTAGC	1 cut(s)	35
AvaII	GGWCC	4 cut(s)	180, 339, 697, 761
BaeGI	GKGCMC	1 cut(s)	821
BalI	TGGCCA	1 cut(s)	855
BanI	GGYRCC	1 cut(s)	528
BanII	GRGCYC	1 cut(s)	616
BbsI	GAAGAC	2 cut(s)	245, 376
Bbv12I	GWGCWC	4 cut(s)	220, 235, 468, 616
BbvCI	CCTCAGC	1 cut(s)	335
BbvI	GCAGC	1 cut(s)	167
BccI	CCATC	1 cut(s)	842
BciT130I	CCWGG	1 cut(s)	828
BcnI	CCSGG	1 cut(s)	922
BcuI	ACTAGT	1 cut(s)	110
BfaI	CTAG	4 cut(s)	36, 111, 213, 617
BfrI	CTTAAG	1 cut(s)	302
BisI	GCNGC	2 cut(s)	156, 784
BlsI	GCNGC	2 cut(s)	157, 785
BmcAI	AGTACT	2 cut(s)	98, 109
Bme1390I	CCNGG	2 cut(s)	828, 922
Bme18I	GGWCC	4 cut(s)	180, 339, 697, 761
BmgT120I	GGNCC	5 cut(s)	180, 339, 697, 761, 923
BmiI	GGNNCC	4 cut(s)	181, 182, 530, 799
BmrFI	CCNGG	2 cut(s)	828, 922
BmrI	ACTGGG	1 cut(s)	186
BmsI	GCATC	4 cut(s)	94, 132, 676, 733
BmtI	GCTAGC	1 cut(s)	39
BmuI	ACTGGG	1 cut(s)	186
BpiI	GAAGAC	2 cut(s)	245, 376
BpmI	CTGGAG	3 cut(s)	85, 317, 659
Bpu10I	CCTNAGC	1 cut(s)	335
BpuMI	CCSGG	1 cut(s)	922
Bsa29I	ATCGAT	1 cut(s)	195
BsaAI	YACGTR	1 cut(s)	28
BsaJI	CCNNGG	2 cut(s)	769, 826
BsaXI	ACNNNNNCTCC	2 cut(s)	290, 320
Bsc4I	CCNNNNNNNGG	4 cut(s)	176, 770, 827, 883
Bse118I	RCCGGY	1 cut(s)	169
Bse1I	ACTGG	2 cut(s)	102, 181
BseBI	CCWGG	1 cut(s)	828
BseCI	ATCGAT	1 cut(s)	195
BseDI	CCNNGG	2 cut(s)	769, 826
BseGI	GGATG	4 cut(s)	85, 379, 748, 853
BseLI	CCNNNNNNNGG	4 cut(s)	176, 770, 827, 883
BseMII	CTCAG	1 cut(s)	326
BseNI	ACTGG	2 cut(s)	102, 181
BseRI	GAGGAG	1 cut(s)	786
BseSI	GKGCMC	1 cut(s)	821
BseXI	GCAGC	1 cut(s)	167
BshFI	GGCC	2 cut(s)	855, 925
BshNI	GGYRCC	1 cut(s)	528
BshVI	ATCGAT	1 cut(s)	195
BsiHKAI	GWGCWC	4 cut(s)	220, 235, 468, 616
BsiSI	CCGG	2 cut(s)	170, 921
BslFI	GGGAC	2 cut(s)	166, 400
BslI	CCNNNNNNNGG	4 cut(s)	176, 770, 827, 883
BsmFI	GGGAC	2 cut(s)	166, 400
BsnI	GGCC	2 cut(s)	855, 925
Bsp1286I	GDGCHC	5 cut(s)	220, 235, 468, 616, 821
Bsp143I	GATC	2 cut(s)	91, 518
BspACI	CCGC	3 cut(s)	342, 783, 786
BspANI	GGCC	2 cut(s)	855, 925
BspCNI	CTCAG	1 cut(s)	327
BspDI	ATCGAT	1 cut(s)	195
BspHI	TCATGA	1 cut(s)	79
BspLI	GGNNCC	4 cut(s)	181, 182, 530, 799
BspOI	GCTAGC	1 cut(s)	39
BspPI	GGATC	1 cut(s)	526
BspQI	GCTCTTC	1 cut(s)	627
BspT107I	GGYRCC	1 cut(s)	528
BspTI	CTTAAG	1 cut(s)	302
BsrFI	RCCGGY	1 cut(s)	169
BsrI	ACTGG	2 cut(s)	102, 181
BssAI	RCCGGY	1 cut(s)	169
BssECI	CCNNGG	2 cut(s)	769, 826
BssMI	GATC	2 cut(s)	91, 518
Bst2UI	CCWGG	1 cut(s)	828
Bst4CI	ACNGT	3 cut(s)	71, 557, 942
Bst6I	CTCTTC	1 cut(s)	627
BstAFI	CTTAAG	1 cut(s)	302
BstBAI	YACGTR	1 cut(s)	28
BstC8I	GCNNGC	3 cut(s)	37, 231, 908
BstDEI	CTNAG	3 cut(s)	11, 335, 484
BstF5I	GGATG	4 cut(s)	85, 379, 748, 853
BstHHI	GCGC	1 cut(s)	691
BstKTI	GATC	2 cut(s)	94, 521
BstMBI	GATC	2 cut(s)	91, 518
BstNI	CCWGG	1 cut(s)	828
BstNSI	RCATGY	1 cut(s)	750
BstSCI	CCNGG	2 cut(s)	826, 920
BstSLI	GKGCMC	1 cut(s)	821
BstV1I	GCAGC	1 cut(s)	167
BstV2I	GAAGAC	2 cut(s)	245, 376
BstX2I	RGATCY	1 cut(s)	518
BstYI	RGATCY	1 cut(s)	518
Bsu15I	ATCGAT	1 cut(s)	195
BsuRI	GGCC	2 cut(s)	855, 925
BsuTUI	ATCGAT	1 cut(s)	195
BtsCI	GGATG	4 cut(s)	85, 379, 748, 853
BtsIMutI	CAGTG	2 cut(s)	137, 561
Cac8I	GCNNGC	3 cut(s)	37, 231, 908
CciI	TCATGA	1 cut(s)	79
CfoI	GCGC	1 cut(s)	691
Cfr10I	RCCGGY	1 cut(s)	169
Cfr13I	GGNCC	5 cut(s)	180, 339, 697, 761, 923
ClaI	ATCGAT	1 cut(s)	195
CseI	GACGC	2 cut(s)	268, 349
Csp6I	GTAC	2 cut(s)	97, 108
CviAII	CATG	4 cut(s)	80, 185, 722, 747
CviQI	GTAC	2 cut(s)	97, 108
DdeI	CTNAG	3 cut(s)	11, 335, 484
DpnI	GATC	2 cut(s)	93, 520
DpnII	GATC	2 cut(s)	91, 518
DraI	TTTAAA	1 cut(s)	276
EaeI	YGGCCR	1 cut(s)	853
Eam1104I	CTCTTC	1 cut(s)	627
EarI	CTCTTC	1 cut(s)	627
EciI	GGCGGA	1 cut(s)	801
Ecl136II	GAGCTC	1 cut(s)	614
Eco24I	GRGCYC	1 cut(s)	616
Eco47I	GGWCC	4 cut(s)	180, 339, 697, 761
Eco53kI	GAGCTC	1 cut(s)	614
Eco57I	CTGAAG	1 cut(s)	585
EcoICRI	GAGCTC	1 cut(s)	614
EcoO109I	RGGNCCY	1 cut(s)	180
EcoRI	GAATTC	1 cut(s)	428
EcoRII	CCWGG	1 cut(s)	826
EcoT22I	ATGCAT	1 cut(s)	748
EcoT38I	GRGCYC	1 cut(s)	616
FaeI	CATG	4 cut(s)	83, 188, 725, 750
FaqI	GGGAC	2 cut(s)	166, 400
FatI	CATG	4 cut(s)	79, 184, 721, 746
Fnu4HI	GCNGC	2 cut(s)	156, 784
FokI	GGATG	4 cut(s)	72, 386, 755, 860
FriOI	GRGCYC	1 cut(s)	616
Fsp4HI	GCNGC	2 cut(s)	156, 784
FspBI	CTAG	4 cut(s)	36, 111, 213, 617
GlaI	GCGC	1 cut(s)	690
GluI	GCNGC	2 cut(s)	156, 784
GsuI	CTGGAG	3 cut(s)	85, 317, 659
HaeIII	GGCC	2 cut(s)	855, 925
HapII	CCGG	2 cut(s)	170, 921
HgaI	GACGC	2 cut(s)	268, 349
HhaI	GCGC	1 cut(s)	691
Hin1II	CATG	4 cut(s)	83, 188, 725, 750
Hin6I	GCGC	1 cut(s)	689
HinP1I	GCGC	1 cut(s)	689
HinfI	GANTC	5 cut(s)	7, 197, 413, 589, 657
HpaII	CCGG	2 cut(s)	170, 921
HphI	GGTGA	3 cut(s)	16, 323, 842
Hpy166II	GTNNAC	2 cut(s)	349, 729
Hpy188I	TCNGA	5 cut(s)	258, 365, 427, 510, 742
Hpy188III	TCNNGA	3 cut(s)	80, 89, 654
Hpy8I	GTNNAC	2 cut(s)	349, 729
Hpy99I	CGWCG	2 cut(s)	262, 365
HpyAV	CCTTC	4 cut(s)	71, 486, 560, 572
HpyCH4III	ACNGT	3 cut(s)	71, 557, 942
HpyCH4IV	ACGT	2 cut(s)	27, 328
HpyCH4V	TGCA	3 cut(s)	503, 716, 746
HpyF3I	CTNAG	3 cut(s)	11, 335, 484
HpySE526I	ACGT	2 cut(s)	27, 328
Hsp92II	CATG	4 cut(s)	83, 188, 725, 750
HspAI	GCGC	1 cut(s)	689
KflI	GGGWCCC	1 cut(s)	180
Kzo9I	GATC	2 cut(s)	91, 518
LguI	GCTCTTC	1 cut(s)	627
LmnI	GCTCC	2 cut(s)	298, 640
Lsp1109I	GCAGC	1 cut(s)	167
LweI	GCATC	4 cut(s)	94, 132, 676, 733
MaeI	CTAG	4 cut(s)	36, 111, 213, 617
MaeII	ACGT	2 cut(s)	27, 328
MaeIII	GTNAC	4 cut(s)	208, 449, 559, 830
MalI	GATC	2 cut(s)	93, 520
MboI	GATC	2 cut(s)	91, 518
MboII	GAAGA	4 cut(s)	245, 376, 508, 644
MflI	RGATCY	1 cut(s)	518
MhlI	GDGCHC	5 cut(s)	220, 235, 468, 616, 821
MlsI	TGGCCA	1 cut(s)	855
MluCI	AATT	4 cut(s)	51, 428, 812, 881
MluNI	TGGCCA	1 cut(s)	855
MlyI	GAGTC	3 cut(s)	16, 422, 583
MmeI	TCCRAC	4 cut(s)	190, 281, 343, 720
MnlI	CCTC	8 cut(s)	157, 161, 330, 359, 428, 482, 638, 764
Mox20I	TGGCCA	1 cut(s)	855
Mph1103I	ATGCAT	1 cut(s)	748
MscI	TGGCCA	1 cut(s)	855
MseI	TTAA	5 cut(s)	266, 275, 303, 675, 879
MslI	CAYNNNNRTG	1 cut(s)	78
Msp20I	TGGCCA	1 cut(s)	855
MspA1I	CMGCKG	1 cut(s)	344
MspCI	CTTAAG	1 cut(s)	302
MspI	CCGG	2 cut(s)	170, 921
MspR9I	CCNGG	2 cut(s)	828, 922
MvaI	CCWGG	1 cut(s)	828
NciI	CCSGG	1 cut(s)	922
NdeII	GATC	2 cut(s)	91, 518
NheI	GCTAGC	1 cut(s)	35
NlaIII	CATG	4 cut(s)	83, 188, 725, 750
NlaIV	GGNNCC	4 cut(s)	181, 182, 530, 799
NmuCI	GTSAC	3 cut(s)	208, 559, 830
NsiI	ATGCAT	1 cut(s)	748
NspI	RCATGY	1 cut(s)	750
PagI	TCATGA	1 cut(s)	79
PciSI	GCTCTTC	1 cut(s)	627
PfeI	GAWTC	2 cut(s)	197, 657
PkrI	GCNGC	2 cut(s)	157, 785
PleI	GAGTC	3 cut(s)	15, 421, 583
PpsI	GAGTC	3 cut(s)	15, 421, 583
Ppu21I	YACGTR	1 cut(s)	28
PpuMI	RGGWCCY	1 cut(s)	180
Psp124BI	GAGCTC	1 cut(s)	616
Psp5II	RGGWCCY	1 cut(s)	180
Psp6I	CCWGG	1 cut(s)	826
PspGI	CCWGG	1 cut(s)	826
PspN4I	GGNNCC	4 cut(s)	181, 182, 530, 799
PspPI	GGNCC	5 cut(s)	180, 339, 697, 761, 923
PspPPI	RGGWCCY	1 cut(s)	180
PsuI	RGATCY	1 cut(s)	518
RsaI	GTAC	2 cut(s)	98, 109
RsaNI	GTAC	2 cut(s)	97, 108
RseI	CAYNNNNRTG	1 cut(s)	78
SacI	GAGCTC	1 cut(s)	616
SapI	GCTCTTC	1 cut(s)	627
SaqAI	TTAA	5 cut(s)	266, 275, 303, 675, 879
SatI	GCNGC	2 cut(s)	156, 784
Sau3AI	GATC	2 cut(s)	91, 518
Sau96I	GGNCC	5 cut(s)	180, 339, 697, 761, 923
ScaI	AGTACT	2 cut(s)	98, 109
SchI	GAGTC	3 cut(s)	16, 422, 583
ScrFI	CCNGG	2 cut(s)	828, 922
SduI	GDGCHC	5 cut(s)	220, 235, 468, 616, 821
SfaNI	GCATC	4 cut(s)	94, 132, 676, 733
SinI	GGWCC	4 cut(s)	180, 339, 697, 761
SmiMI	CAYNNNNRTG	1 cut(s)	78
SmlI	CTYRAG	1 cut(s)	302
SmoI	CTYRAG	1 cut(s)	302
SpeI	ACTAGT	1 cut(s)	110
Sse9I	AATT	4 cut(s)	51, 428, 812, 881
SsiI	CCGC	3 cut(s)	342, 783, 786
SspI	AATATT	2 cut(s)	665, 672
SspMI	CTAG	4 cut(s)	36, 111, 213, 617
SstI	GAGCTC	1 cut(s)	616
StyD4I	CCNGG	2 cut(s)	826, 920
TaaI	ACNGT	3 cut(s)	71, 557, 942
TaiI	ACGT	2 cut(s)	30, 331
TaqI	TCGA	3 cut(s)	195, 630, 860
TaqII	GACCGA	1 cut(s)	473
TasI	AATT	4 cut(s)	51, 428, 812, 881
TatI	WGTACW	2 cut(s)	96, 107
TauI	GCSGC	1 cut(s)	786
TfiI	GAWTC	2 cut(s)	197, 657
Tru1I	TTAA	5 cut(s)	266, 275, 303, 675, 879
Tru9I	TTAA	5 cut(s)	266, 275, 303, 675, 879
TscAI	CASTG	2 cut(s)	144, 568
TseFI	GTSAC	3 cut(s)	208, 559, 830
TseI	GCWGC	1 cut(s)	155
Tsp45I	GTSAC	3 cut(s)	208, 559, 830
TspDTI	ATGAA	1 cut(s)	421
TspRI	CASTG	2 cut(s)	144, 568
Vha464I	CTTAAG	1 cut(s)	302
VpaK11BI	GGWCC	4 cut(s)	180, 339, 697, 761
XapI	RAATTY	1 cut(s)	428
XceI	RCATGY	1 cut(s)	750
XcmI	CCANNNNNNNNNTGG	1 cut(s)	173
XspI	CTAG	4 cut(s)	36, 111, 213, 617
ZrmI	AGTACT	2 cut(s)	98, 109
Zsp2I	ATGCAT	1 cut(s)	748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.