Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0000443

Physical Location & Seq

Genomic Coordinates Forward (+)

47772502 .. 47779577

Gene Structure 7076 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G030220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGGCAAGTCTTAGCTACATTGTTTATGTGTTGGCAAGCCTACTCAACCCAGTTAGCTTCCTTCTTTATGATCATCATAGTCGGTATACTGGTGGTAGTAATAGTAGTATAGAACATGATGATCAATATAAGGTGGCATTGTTTGTGTTCGGAGACTCGCTATTTGAGGCTGTAGTGAATGGGTACCTTCCCAATATTACCACAGAGCTAGACCCAGCAATAGCCTGGCCATATGGAGAAACCTTCTTCCACTATCCGACCGGAAGATTCTCCGACGGACGCATAGTCCCCGATTTCATCGCTAAGTACGCAAAGCTGCCCATGCTTCCACCTTACCTGCAACCAGGTCCACATGATTTTACCGATGGATGCAACTTTGCTTCGTCTGGAGCCGGGGTTCTTGTTGAAACTAATCCAGGAAAATCGATAAGTCTTCCAGCACAGTTGGGCTTCTTCAGAGATGTCAGAAAGTCGCTACAACAGAAATTGGGTGGTCTAAAAACCAAACGCGTGCTCAGGGAAGCTGTTTACTTGATTGGCATCGGAGGGGATGATTATTTTCACTTCTACAGCGACTTTCCAAATGCCACCGAGTCCCTTCAACTCGAAGCCGTAGCAGTTGAAATATATGATTTAGGAGGAAGGAAGTTTGCATTTCAAAATGCAGGACCGCTTGGATGCATACCTTATAGGAGACAAATTTACAATACTAAGGAAGGATGCGTTGAAGGACTCAATAGTCTGGCTAGTCTACATAACACAGCTCTAGTTTCTGTCCTTAGAGAGTTCGAGAGCCAACTATTAGGATTCAAATATTCAATATTTGATTACTATAATTCACTTGATGATCGACTCCGTAACCCTGCCAAATATGGGTTCACAAATGCGACAGATGCATGTTGTGGTACAGGGCTGTTCAGAGCAGCTTATTGTGGTGGAGATAATGGAACAAAACCATATGAACTATGCAATGACGTTGGAGATTATTTGTGGTTTGATGGTAGCCATACGACTGAAATTGCAAACTTTCCAATGGCAGAGCTGATGTGGAGTGGAACACCCAATGTCACAGGGCCTTACAATGTCAAACAACTTTTCCAACAAGTTTCAGCTTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005840 GO:0005911 GO:0006082 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009409 GO:0009505 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009941 GO:0009987 GO:0016020 GO:0016143 GO:0016145 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0022626 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032991 GO:0042579 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0044237 GO:0044248 GO:0044273 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_GDSL

Protein Analysis

372

Amino Acids

41.28

Weight (kDa)

5.27

Isoelectric Point (pI)

31.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_GDSL	PF00657	47 - 347	4e-25	GDSL-like Lipase/Acylhydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000443)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35490 FvH4_6g35490 FvH4_6g35500 FvH4_6g35500 FvH4_6g35500 FvH4_6g35520 FvH4_6g35520 FvH4_6g35540 FvH4_6g35550
malus_domestica	MD09G1175400.v1.1
pyrus_communis	pycom09g09260
rosa_chinensis	RchiOBHm_Chr2g0144821 RchiOBHm_Chr2g0145011 RchiOBHm_Chr2g0145061 RchiOBHm_Chr2g0147051 RchiOBHm_Chr2g0147071 RchiOBHm_Chr2g0147111 RchiOBHm_Chr2g0147141
rosa_laevigata	RLG00000020116 RLG00000020119 RLG00000020120 RLG00000020122 RLG00000020243 RLG00000020245 RLG00000020246
rosa_multiflora	Rmu_co8407447.1_g000001 Rmu_sc0000070.1_g000059 Rmu_sc0001668.1_g000005 Rmu_sc0001880.1_g000013 Rmu_sc0001880.1_g000020 Rmu_sc0001880.1_g000023 Rmu_sc0001880.1_g000027 Rmu_sc0002354.1_g000023 Rmu_sc0002506.1_g000001 Rmu_sc0002506.1_g000016 Rmu_sc0004897.1_g000008 Rmu_sc0007988.1_g000001
rosa_roxburghii	Rroxscaffold_1G00033230 Rroxscaffold_2G00099670 Rroxscaffold_2G00099720 Rroxscaffold_2G00100900 Rroxscaffold_2G00100930 Rroxscaffold_2G00100940
rosa_rugosa	Rorug02G0390000 Rorug02G0390100 Rorug02G0390200 Rorug02G0390200 Rorug02G0390200 Rorug02G0398400 Rorug02G0398500 Rorug02G0398600
rosa_samantha	Rh2AG442200 Rh2AG442500 Rh2AG442700 Rh2AG455600 Rh2AG455700 Rh2AG455800 Rh2AG455900 Rh2BG452500 Rh2BG453000 Rh2BG453700 Rh2BG467900 Rh2BG468100 Rh2BG468200 Rh2CG429200 Rh2CG429500 Rh2CG429600 Rh2CG429700 Rh2CG443000 Rh2CG443100 Rh2CG443200 Rh2CG443300 Rh2DG462200 Rh2DG463000 Rh2DG463200 Rh2DG463700 Rh2DG477300 Rh2DG477500 Rh2DG477600 Rh4AG390700 Rh5AG320200
rosa_wichuraiana	Rw2G036130 Rw2G036170 Rw2G036180 Rw2G037150 Rw2G037160 Rw2G037170 Rw2G037180 Rw2G037260 Rw2G037270 Rw2G037280 Rw2G037290 Rw5G030070 Rw5G030220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	738
Acc36I	ACCTGC	1 cut(s)	345
Acc65I	GGTACC	1 cut(s)	183
AccB1I	GGYRCC	1 cut(s)	183
AccI	GTMKAC	2 cut(s)	86, 751
AccII	CGCG	1 cut(s)	510
AciI	CCGC	1 cut(s)	671
AcoI	YGGCCR	1 cut(s)	227
AcsI	RAATTY	1 cut(s)	699
AcuI	CTGAAG	1 cut(s)	439
AfaI	GTAC	3 cut(s)	185, 308, 907
AfiI	CCNNNNNNNGG	1 cut(s)	873
AflIII	ACRYGT	1 cut(s)	508
AgsI	TTSAA	7 cut(s)	407, 602, 623, 659, 728, 811, 819
AhdI	GACNNNNNGTC	1 cut(s)	284
AjnI	CCWGG	3 cut(s)	224, 343, 415
AjuI	GAANNNNNNNTTGG	2 cut(s)	860, 892
AloI	GAACNNNNNNTCC	2 cut(s)	381, 413
AluBI	AGCT	9 cut(s)	15, 57, 208, 316, 524, 764, 926, 1042, 1112
AluI	AGCT	9 cut(s)	15, 57, 208, 316, 524, 764, 926, 1042, 1112
Alw21I	GWGCWC	1 cut(s)	516
Alw26I	GTCTC	2 cut(s)	147, 688
AoxI	GGCC	2 cut(s)	227, 1073
ApeKI	GCWGC	2 cut(s)	316, 923
ApoI	RAATTY	1 cut(s)	699
ArsI	GACNNNNNNTTYG	2 cut(s)	146, 178
Asp718I	GGTACC	1 cut(s)	183
AspS9I	GGNCC	3 cut(s)	347, 668, 1073
AsuC2I	CCSGG	1 cut(s)	394
AvaII	GGWCC	2 cut(s)	347, 668
BalI	TGGCCA	1 cut(s)	229
BanI	GGYRCC	1 cut(s)	183
BbsI	GAAGAC	1 cut(s)	425
Bbv12I	GWGCWC	1 cut(s)	516
BbvI	GCAGC	2 cut(s)	303, 935
BccI	CCATC	2 cut(s)	359, 992
BceAI	ACGGC	1 cut(s)	596
BciT130I	CCWGG	3 cut(s)	226, 345, 417
BclI	TGATCA	2 cut(s)	70, 121
BcnI	CCSGG	1 cut(s)	394
BcoDI	GTCTC	2 cut(s)	147, 688
BfaI	CTAG	3 cut(s)	209, 747, 767
BfmI	CTRYAG	2 cut(s)	171, 568
BfuAI	ACCTGC	1 cut(s)	345
BisI	GCNGC	2 cut(s)	317, 924
BlsI	GCNGC	2 cut(s)	318, 925
Bme1390I	CCNGG	4 cut(s)	226, 345, 394, 417
Bme18I	GGWCC	2 cut(s)	347, 668
BmeRI	GACNNNNNGTC	1 cut(s)	284
BmgT120I	GGNCC	3 cut(s)	347, 668, 1073
BmiI	GGNNCC	2 cut(s)	185, 391
BmrFI	CCNGG	4 cut(s)	226, 345, 394, 417
BmrI	ACTGGG	1 cut(s)	44
BmsI	GCATC	5 cut(s)	359, 549, 668, 710, 883
BmuI	ACTGGG	1 cut(s)	44
BpiI	GAAGAC	1 cut(s)	425
BpmI	CTGGAG	1 cut(s)	408
Bpu10I	CCTNAGC	1 cut(s)	515
BpuMI	CCSGG	1 cut(s)	394
Bsa29I	ATCGAT	1 cut(s)	425
BsaBI	GATNNNNATC	1 cut(s)	539
BsaJI	CCNNGG	1 cut(s)	393
BsaWI	WCCGGW	1 cut(s)	260
BsaXI	ACNNNNNCTCC	2 cut(s)	381, 411
Bsc4I	CCNNNNNNNGG	1 cut(s)	873
Bse1I	ACTGG	2 cut(s)	50, 94
Bse3DI	GCAATG	1 cut(s)	976
Bse8I	GATNNNNATC	1 cut(s)	539
BseBI	CCWGG	3 cut(s)	226, 345, 417
BseCI	ATCGAT	1 cut(s)	425
BseDI	CCNNGG	1 cut(s)	393
BseGI	GGATG	4 cut(s)	374, 556, 683, 725
BseJI	GATNNNNATC	1 cut(s)	539
BseLI	CCNNNNNNNGG	1 cut(s)	873
BseMI	GCAATG	1 cut(s)	976
BseMII	CTCAG	1 cut(s)	529
BseNI	ACTGG	2 cut(s)	50, 94
BseXI	GCAGC	2 cut(s)	303, 935
BseYI	CCCAGC	1 cut(s)	214
Bsh1236I	CGCG	1 cut(s)	510
Bsh1285I	CGRYCG	1 cut(s)	261
BshFI	GGCC	2 cut(s)	229, 1075
BshNI	GGYRCC	1 cut(s)	183
BshVI	ATCGAT	1 cut(s)	425
BsiEI	CGRYCG	1 cut(s)	261
BsiHKAI	GWGCWC	1 cut(s)	516
BsiSI	CCGG	2 cut(s)	261, 393
BslFI	GGGAC	2 cut(s)	272, 580
BslI	CCNNNNNNNGG	1 cut(s)	873
BsmAI	GTCTC	2 cut(s)	147, 688
BsmFI	GGGAC	2 cut(s)	272, 580
BsnI	GGCC	2 cut(s)	229, 1075
Bsp1286I	GDGCHC	1 cut(s)	516
Bsp143I	GATC	3 cut(s)	70, 121, 847
BspACI	CCGC	1 cut(s)	671
BspANI	GGCC	2 cut(s)	229, 1075
BspCNI	CTCAG	1 cut(s)	528
BspDI	ATCGAT	1 cut(s)	425
BspFNI	CGCG	1 cut(s)	510
BspLI	GGNNCC	2 cut(s)	185, 391
BspMI	ACCTGC	1 cut(s)	345
BspT107I	GGYRCC	1 cut(s)	183
BsrDI	GCAATG	1 cut(s)	976
BsrI	ACTGG	2 cut(s)	50, 94
BssECI	CCNNGG	1 cut(s)	393
BssMI	GATC	3 cut(s)	70, 121, 847
BssNAI	GTATAC	1 cut(s)	87
Bst1107I	GTATAC	1 cut(s)	87
Bst2UI	CCWGG	3 cut(s)	226, 345, 417
Bst4CI	ACNGT	1 cut(s)	444
BstC8I	GCNNGC	2 cut(s)	37, 512
BstDEI	CTNAG	5 cut(s)	11, 303, 515, 711, 779
BstF5I	GGATG	4 cut(s)	374, 556, 683, 725
BstFNI	CGCG	1 cut(s)	510
BstKTI	GATC	3 cut(s)	73, 124, 850
BstMAI	GTCTC	2 cut(s)	147, 688
BstMBI	GATC	3 cut(s)	70, 121, 847
BstMCI	CGRYCG	1 cut(s)	261
BstMWI	GCNNNNNNNGC	3 cut(s)	308, 322, 893
BstNI	CCWGG	3 cut(s)	226, 345, 417
BstNSI	RCATGY	1 cut(s)	900
BstSCI	CCNGG	4 cut(s)	224, 343, 392, 415
BstSFI	CTRYAG	2 cut(s)	171, 568
BstUI	CGCG	1 cut(s)	510
BstV1I	GCAGC	2 cut(s)	303, 935
BstV2I	GAAGAC	1 cut(s)	425
BstZ17I	GTATAC	1 cut(s)	87
Bsu15I	ATCGAT	1 cut(s)	425
BsuRI	GGCC	2 cut(s)	229, 1075
BsuTUI	ATCGAT	1 cut(s)	425
BtgZI	GCGATG	1 cut(s)	283
BtsCI	GGATG	4 cut(s)	374, 556, 683, 725
BveI	ACCTGC	1 cut(s)	345
Cac8I	GCNNGC	2 cut(s)	37, 512
Cfr13I	GGNCC	3 cut(s)	347, 668, 1073
ClaI	ATCGAT	1 cut(s)	425
CseI	GACGC	1 cut(s)	288
CsiI	ACCWGGT	1 cut(s)	343
Csp6I	GTAC	3 cut(s)	184, 307, 906
CviAII	CATG	4 cut(s)	116, 322, 353, 897
CviQI	GTAC	3 cut(s)	184, 307, 906
DdeI	CTNAG	5 cut(s)	11, 303, 515, 711, 779
DpnI	GATC	3 cut(s)	72, 123, 849
DpnII	GATC	3 cut(s)	70, 121, 847
DrdI	GACNNNNNNGTC	1 cut(s)	738
DriI	GACNNNNNGTC	1 cut(s)	284
DseDI	GACNNNNNNGTC	1 cut(s)	738
EaeI	YGGCCR	1 cut(s)	227
Eam1105I	GACNNNNNGTC	1 cut(s)	284
Eco47I	GGWCC	2 cut(s)	347, 668
Eco57I	CTGAAG	1 cut(s)	439
EcoO109I	RGGNCCY	1 cut(s)	1073
EcoRII	CCWGG	3 cut(s)	224, 343, 415
EcoT22I	ATGCAT	2 cut(s)	683, 898
FaeI	CATG	4 cut(s)	119, 325, 356, 900
FaqI	GGGAC	2 cut(s)	272, 580
FatI	CATG	4 cut(s)	115, 321, 352, 896
FauNDI	CATATG	2 cut(s)	232, 958
FbaI	TGATCA	2 cut(s)	70, 121
FblI	GTMKAC	2 cut(s)	86, 751
Fnu4HI	GCNGC	2 cut(s)	317, 924
FokI	GGATG	4 cut(s)	381, 563, 690, 732
Fsp4HI	GCNGC	2 cut(s)	317, 924
FspBI	CTAG	3 cut(s)	209, 747, 767
GluI	GCNGC	2 cut(s)	317, 924
GsaI	CCCAGC	1 cut(s)	218
GsuI	CTGGAG	1 cut(s)	408
HaeIII	GGCC	2 cut(s)	229, 1075
HapII	CCGG	2 cut(s)	261, 393
HgaI	GACGC	1 cut(s)	288
Hin1II	CATG	4 cut(s)	119, 325, 356, 900
HinfI	GANTC	6 cut(s)	155, 267, 593, 732, 807, 852
HpaII	CCGG	2 cut(s)	261, 393
Hpy166II	GTNNAC	5 cut(s)	87, 350, 529, 752, 879
Hpy188I	TCNGA	7 cut(s)	152, 258, 274, 458, 467, 545, 920
Hpy188III	TCNNGA	2 cut(s)	387, 790
Hpy8I	GTNNAC	5 cut(s)	87, 350, 529, 752, 879
Hpy99I	CGWCG	1 cut(s)	278
HpyAV	CCTTC	7 cut(s)	71, 197, 253, 608, 636, 710, 722
HpyCH4III	ACNGT	1 cut(s)	444
HpyCH4IV	ACGT	1 cut(s)	975
HpyCH4V	TGCA	8 cut(s)	340, 372, 653, 665, 681, 896, 969, 1022
HpyF10VI	GCNNNNNNNGC	3 cut(s)	308, 322, 893
HpyF3I	CTNAG	5 cut(s)	11, 303, 515, 711, 779
HpySE526I	ACGT	1 cut(s)	975
Hsp92II	CATG	4 cut(s)	119, 325, 356, 900
KpnI	GGTACC	1 cut(s)	187
Ksp22I	TGATCA	2 cut(s)	70, 121
Kzo9I	GATC	3 cut(s)	70, 121, 847
LmnI	GCTCC	1 cut(s)	389
Lsp1109I	GCAGC	2 cut(s)	303, 935
LweI	GCATC	5 cut(s)	359, 549, 668, 710, 883
MabI	ACCWGGT	1 cut(s)	343
MaeI	CTAG	3 cut(s)	209, 747, 767
MaeII	ACGT	1 cut(s)	975
MaeIII	GTNAC	2 cut(s)	857, 1066
MalI	GATC	3 cut(s)	72, 123, 849
MboI	GATC	3 cut(s)	70, 121, 847
MboII	GAAGA	4 cut(s)	238, 276, 425, 445
MhlI	GDGCHC	1 cut(s)	516
MlsI	TGGCCA	1 cut(s)	229
MluCI	AATT	4 cut(s)	485, 699, 835, 1017
MluI	ACGCGT	1 cut(s)	508
MluNI	TGGCCA	1 cut(s)	229
MlyI	GAGTC	4 cut(s)	149, 602, 726, 846
MmeI	TCCRAC	3 cut(s)	281, 297, 958
MnlI	CCTC	3 cut(s)	160, 539, 632
Mox20I	TGGCCA	1 cut(s)	229
Mph1103I	ATGCAT	2 cut(s)	683, 898
MscI	TGGCCA	1 cut(s)	229
MseI	TTAA	1 cut(s)	1117
Msp20I	TGGCCA	1 cut(s)	229
MspI	CCGG	2 cut(s)	261, 393
MspR9I	CCNGG	4 cut(s)	226, 345, 394, 417
MvaI	CCWGG	3 cut(s)	226, 345, 417
MvnI	CGCG	1 cut(s)	510
MwoI	GCNNNNNNNGC	3 cut(s)	308, 322, 893
NciI	CCSGG	1 cut(s)	394
NdeI	CATATG	2 cut(s)	232, 958
NdeII	GATC	3 cut(s)	70, 121, 847
NlaIII	CATG	4 cut(s)	119, 325, 356, 900
NlaIV	GGNNCC	2 cut(s)	185, 391
NmuCI	GTSAC	1 cut(s)	1066
NsiI	ATGCAT	2 cut(s)	683, 898
NspI	RCATGY	1 cut(s)	900
PfeI	GAWTC	2 cut(s)	267, 807
PfoI	TCCNGGA	1 cut(s)	415
PkrI	GCNGC	2 cut(s)	318, 925
PleI	GAGTC	4 cut(s)	149, 601, 726, 846
PpsI	GAGTC	4 cut(s)	149, 601, 726, 846
Psp6I	CCWGG	3 cut(s)	224, 343, 415
PspFI	CCCAGC	1 cut(s)	214
PspGI	CCWGG	3 cut(s)	224, 343, 415
PspN4I	GGNNCC	2 cut(s)	185, 391
PspPI	GGNCC	3 cut(s)	347, 668, 1073
RsaI	GTAC	3 cut(s)	185, 308, 907
RsaNI	GTAC	3 cut(s)	184, 307, 906
SaqAI	TTAA	1 cut(s)	1117
SatI	GCNGC	2 cut(s)	317, 924
Sau3AI	GATC	3 cut(s)	70, 121, 847
Sau96I	GGNCC	3 cut(s)	347, 668, 1073
SchI	GAGTC	4 cut(s)	149, 602, 726, 846
ScrFI	CCNGG	4 cut(s)	226, 345, 394, 417
SduI	GDGCHC	1 cut(s)	516
SexAI	ACCWGGT	1 cut(s)	343
SfaNI	GCATC	5 cut(s)	359, 549, 668, 710, 883
SfcI	CTRYAG	2 cut(s)	171, 568
SinI	GGWCC	2 cut(s)	347, 668
Sse9I	AATT	4 cut(s)	485, 699, 835, 1017
SsiI	CCGC	1 cut(s)	671
SspI	AATATT	3 cut(s)	196, 815, 822
SspMI	CTAG	3 cut(s)	209, 747, 767
StyD4I	CCNGG	4 cut(s)	224, 343, 392, 415
TaaI	ACNGT	1 cut(s)	444
TaiI	ACGT	1 cut(s)	978
TaqI	TCGA	4 cut(s)	425, 606, 789, 850
TasI	AATT	4 cut(s)	485, 699, 835, 1017
TfiI	GAWTC	2 cut(s)	267, 807
Tru1I	TTAA	1 cut(s)	1117
Tru9I	TTAA	1 cut(s)	1117
TseFI	GTSAC	1 cut(s)	1066
TseI	GCWGC	2 cut(s)	316, 923
Tsp45I	GTSAC	1 cut(s)	1066
TspDTI	ATGAA	2 cut(s)	286, 975
TspGWI	ACGGA	2 cut(s)	291, 845
VpaK11BI	GGWCC	2 cut(s)	347, 668
XapI	RAATTY	1 cut(s)	699
XceI	RCATGY	1 cut(s)	900
XcmI	CCANNNNNNNNNTGG	1 cut(s)	222
XmiI	GTMKAC	2 cut(s)	86, 751
XspI	CTAG	3 cut(s)	209, 747, 767
Zsp2I	ATGCAT	2 cut(s)	683, 898

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.