Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000443

Physical Location & Seq

Genomic Coordinates Reverse (-)

59254005 .. 59266570

Gene Structure 12566 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G036130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1140 bp

ATGGCAAGTCTTAGCTACATTGTTTACGTGTTGGCCAGCCTATTCAACCCAATTACCTTCCTTCTATACGGTCATCGTGATCATGGCGATCAGTACTACAGTAGTACTAGTATAGAACACAATCAAAGTAAGGTGGCGCTGTTTGTGTTTGGGGACTCACCATTTGAGGCTGGAAACGACCAATACCTAATTCCTAATGTTACCGTAGAAGATGATGAAGCAATAGCCTGGCCATATGGAATGACTTTCTTCCACCATCCTACTGGAAGATTCAGCGATGGGCGTATTGTCCCCGATTTTATCGACGCTTCAACGGCTAAGTTTGCAAAGCTGCCGATGCTTCCACCATACTTGCAACCCGGTCCACATGATTTTACCGATGGATCCAACTTCGCTTCGGGTGGAGCGGGGGCCCTTGCAACAACCAACACAGGAACTGCAAGTTTTCCAATGCAGTTGAGTTATTTTAGGAATGTGACAAAGTTGCTACAACAGAAACTGGGTCATTTAGAAGCCAAGCGAATTCTGAGAAACGCTGTGTACTTGATTAGCATCGGAGGCTGTGATTATTTCAGCTTCTATGCTACAAACCCAAATGCGACCGAGCCCCAGCAAGAGGAATATGTAGCAATCGTGATTGGTAACTTGACCACTGTGCTTCAAGGGATATATAACTTAGGAGGAAGGAAAATTGCATTTCAGAATGTGGGATCTCTTGGGTGCCTACCTGCAATGAGACAAATATTCGGTGTTACTGAAGGCTGCGTTGAAGGACTTCTGAGTTTGGCGAACCTACACAATACAGCTCTAGCTAATGTCCTCAAAGAGTTAGAGAGCCAACTATCAGGATTCAAATATTCAATATTTAACTACTATGATGCGACTGAGGATCGATACCTTAACCCTGCAACATATGGGTTCACAAATGGGTCGGATGGATGCTGTGGTACAGGACCGTACAGGGGAGTTGATTGTGCAATAGAACCGTTTGAATTATGCGAAGACCCACCAGAGTATGTGTGGTTTGATGGTGGCCATTCGACTGAAAGGGCAAACTTCCAATATGCAAAGCTAATATGGAGTGGAACCCCGAATACCACAGGGCCTTACAATGTCCAACAGCTTTTTCAGCAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005840 GO:0005911 GO:0006082 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009409 GO:0009505 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009941 GO:0009987 GO:0016020 GO:0016143 GO:0016145 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0022626 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032991 GO:0042579 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0044237 GO:0044248 GO:0044273 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_GDSL

Protein Analysis

379

Amino Acids

41.78

Weight (kDa)

4.92

Isoelectric Point (pI)

30.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_GDSL	PF00657	47 - 357	1.3e-24	GDSL-like Lipase/Acylhydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000443)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35490 FvH4_6g35490 FvH4_6g35500 FvH4_6g35500 FvH4_6g35500 FvH4_6g35520 FvH4_6g35520 FvH4_6g35540 FvH4_6g35550
malus_domestica	MD09G1175400.v1.1
pyrus_communis	pycom09g09260
rosa_chinensis	RchiOBHm_Chr2g0144821 RchiOBHm_Chr2g0145011 RchiOBHm_Chr2g0145061 RchiOBHm_Chr2g0147051 RchiOBHm_Chr2g0147071 RchiOBHm_Chr2g0147111 RchiOBHm_Chr2g0147141
rosa_laevigata	RLG00000020116 RLG00000020119 RLG00000020120 RLG00000020122 RLG00000020243 RLG00000020245 RLG00000020246
rosa_multiflora	Rmu_co8407447.1_g000001 Rmu_sc0000070.1_g000059 Rmu_sc0001668.1_g000005 Rmu_sc0001880.1_g000013 Rmu_sc0001880.1_g000020 Rmu_sc0001880.1_g000023 Rmu_sc0001880.1_g000027 Rmu_sc0002354.1_g000023 Rmu_sc0002506.1_g000001 Rmu_sc0002506.1_g000016 Rmu_sc0004897.1_g000008 Rmu_sc0007988.1_g000001
rosa_roxburghii	Rroxscaffold_1G00033230 Rroxscaffold_2G00099670 Rroxscaffold_2G00099720 Rroxscaffold_2G00100900 Rroxscaffold_2G00100930 Rroxscaffold_2G00100940
rosa_rugosa	Rorug02G0390000 Rorug02G0390100 Rorug02G0390200 Rorug02G0390200 Rorug02G0390200 Rorug02G0398400 Rorug02G0398500 Rorug02G0398600
rosa_samantha	Rh2AG442200 Rh2AG442500 Rh2AG442700 Rh2AG455600 Rh2AG455700 Rh2AG455800 Rh2AG455900 Rh2BG452500 Rh2BG453000 Rh2BG453700 Rh2BG467900 Rh2BG468100 Rh2BG468200 Rh2CG429200 Rh2CG429500 Rh2CG429600 Rh2CG429700 Rh2CG443000 Rh2CG443100 Rh2CG443200 Rh2CG443300 Rh2DG462200 Rh2DG463000 Rh2DG463200 Rh2DG463700 Rh2DG477300 Rh2DG477500 Rh2DG477600 Rh4AG390700 Rh5AG320200
rosa_wichuraiana	Rw2G036130 Rw2G036170 Rw2G036180 Rw2G037150 Rw2G037160 Rw2G037170 Rw2G037180 Rw2G037260 Rw2G037270 Rw2G037280 Rw2G037290 Rw5G030070 Rw5G030220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	736
AccB1I	GGYRCC	1 cut(s)	720
AccBSI	CCGCTC	1 cut(s)	407
AciI	CCGC	1 cut(s)	407
AclWI	GGATC	4 cut(s)	378, 391, 718, 897
AcoI	YGGCCR	3 cut(s)	33, 230, 1033
AcsI	RAATTY	1 cut(s)	522
AcuI	CTGAAG	1 cut(s)	777
AfaI	GTAC	5 cut(s)	95, 106, 542, 949, 959
AfiI	CCNNNNNNNGG	2 cut(s)	616, 962
AflIII	ACRYGT	1 cut(s)	27
AgsI	TTSAA	7 cut(s)	46, 312, 662, 770, 853, 861, 992
AhlI	ACTAGT	1 cut(s)	107
AjnI	CCWGG	1 cut(s)	227
AjuI	GAANNNNNNNTTGG	3 cut(s)	174, 206, 1110
AluBI	AGCT	7 cut(s)	15, 331, 576, 806, 812, 1072, 1123
AluI	AGCT	7 cut(s)	15, 331, 576, 806, 812, 1072, 1123
Alw26I	GTCTC	1 cut(s)	730
AlwI	GGATC	4 cut(s)	378, 391, 718, 897
AlwNI	CAGNNNCTG	2 cut(s)	437, 499
AoxI	GGCC	5 cut(s)	33, 230, 411, 1033, 1103
ApaI	GGGCCC	1 cut(s)	415
ApeKI	GCWGC	2 cut(s)	331, 762
ApoI	RAATTY	1 cut(s)	522
ArsI	GACNNNNNNTTYG	2 cut(s)	146, 178
Asp700I	GAANNNNTTC	1 cut(s)	774
AspLEI	GCGC	1 cut(s)	139
AspS9I	GGNCC	5 cut(s)	362, 411, 412, 953, 1103
AsuC2I	CCSGG	1 cut(s)	360
AsuHPI	GGTGA	1 cut(s)	150
AvaII	GGWCC	2 cut(s)	362, 953
BaeGI	GKGCMC	1 cut(s)	415
BalI	TGGCCA	3 cut(s)	35, 232, 1035
BamHI	GGATCC	1 cut(s)	383
BanI	GGYRCC	1 cut(s)	720
BanII	GRGCYC	2 cut(s)	415, 609
BbsI	GAAGAC	1 cut(s)	1008
BbvI	GCAGC	2 cut(s)	318, 749
BccI	CCATC	5 cut(s)	264, 272, 374, 929, 1022
BceAI	ACGGC	1 cut(s)	330
BciT130I	CCWGG	1 cut(s)	229
BclI	TGATCA	1 cut(s)	79
BcnI	CCSGG	1 cut(s)	360
BcoDI	GTCTC	1 cut(s)	730
BcuI	ACTAGT	1 cut(s)	107
BfaI	CTAG	2 cut(s)	108, 809
BfmI	CTRYAG	1 cut(s)	97
BfoI	RGCGCY	1 cut(s)	140
BfuAI	ACCTGC	1 cut(s)	736
BisI	GCNGC	2 cut(s)	332, 763
BlsI	GCNGC	2 cut(s)	333, 764
BmcAI	AGTACT	2 cut(s)	95, 106
Bme1390I	CCNGG	2 cut(s)	229, 360
Bme18I	GGWCC	2 cut(s)	362, 953
BmgT120I	GGNCC	5 cut(s)	362, 411, 412, 953, 1103
BmiI	GGNNCC	5 cut(s)	385, 412, 413, 722, 1087
BmrFI	CCNGG	2 cut(s)	229, 360
BmrI	ACTGGG	1 cut(s)	509
BmsI	GCATC	4 cut(s)	327, 561, 868, 929
BmuI	ACTGGG	1 cut(s)	509
BpiI	GAAGAC	1 cut(s)	1008
BpuMI	CCSGG	1 cut(s)	360
Bsa29I	ATCGAT	1 cut(s)	892
BsaAI	YACGTR	1 cut(s)	28
BsaBI	GATNNNNATC	1 cut(s)	551
Bsc4I	CCNNNNNNNGG	2 cut(s)	616, 962
Bse1I	ACTGG	2 cut(s)	268, 504
Bse3DI	GCAATG	1 cut(s)	738
Bse8I	GATNNNNATC	1 cut(s)	551
BseBI	CCWGG	1 cut(s)	229
BseCI	ATCGAT	1 cut(s)	892
BseGI	GGATG	3 cut(s)	256, 940, 944
BseJI	GATNNNNATC	1 cut(s)	551
BseLI	CCNNNNNNNGG	2 cut(s)	616, 962
BseMI	GCAATG	1 cut(s)	738
BseMII	CTCAG	3 cut(s)	518, 770, 876
BseNI	ACTGG	2 cut(s)	268, 504
BseSI	GKGCMC	1 cut(s)	415
BseXI	GCAGC	2 cut(s)	318, 749
BseYI	CCCAGC	1 cut(s)	609
Bsh1285I	CGRYCG	1 cut(s)	603
BshFI	GGCC	5 cut(s)	35, 232, 413, 1035, 1105
BshNI	GGYRCC	1 cut(s)	720
BshVI	ATCGAT	1 cut(s)	892
BsiEI	CGRYCG	1 cut(s)	603
BsiSI	CCGG	1 cut(s)	360
BslFI	GGGAC	2 cut(s)	167, 275
BslI	CCNNNNNNNGG	2 cut(s)	616, 962
BsmAI	GTCTC	1 cut(s)	730
BsmFI	GGGAC	2 cut(s)	167, 275
BsnI	GGCC	5 cut(s)	35, 232, 413, 1035, 1105
Bsp120I	GGGCCC	1 cut(s)	411
Bsp1286I	GDGCHC	2 cut(s)	415, 609
Bsp143I	GATC	5 cut(s)	79, 88, 383, 710, 889
BspACI	CCGC	1 cut(s)	407
BspANI	GGCC	5 cut(s)	35, 232, 413, 1035, 1105
BspCNI	CTCAG	3 cut(s)	519, 771, 877
BspDI	ATCGAT	1 cut(s)	892
BspLI	GGNNCC	5 cut(s)	385, 412, 413, 722, 1087
BspMI	ACCTGC	1 cut(s)	736
BspPI	GGATC	4 cut(s)	378, 391, 718, 897
BspT107I	GGYRCC	1 cut(s)	720
BsrBI	CCGCTC	1 cut(s)	407
BsrDI	GCAATG	1 cut(s)	738
BsrI	ACTGG	2 cut(s)	268, 504
BssMI	GATC	5 cut(s)	79, 88, 383, 710, 889
Bst2UI	CCWGG	1 cut(s)	229
Bst4CI	ACNGT	6 cut(s)	71, 101, 205, 655, 957, 987
BstBAI	YACGTR	1 cut(s)	28
BstC8I	GCNNGC	1 cut(s)	37
BstDEI	CTNAG	6 cut(s)	11, 318, 527, 676, 779, 885
BstF5I	GGATG	3 cut(s)	256, 940, 944
BstH2I	RGCGCY	1 cut(s)	140
BstHHI	GCGC	1 cut(s)	139
BstKTI	GATC	5 cut(s)	82, 91, 386, 713, 892
BstMAI	GTCTC	1 cut(s)	730
BstMBI	GATC	5 cut(s)	79, 88, 383, 710, 889
BstMCI	CGRYCG	1 cut(s)	603
BstMWI	GCNNNNNNNGC	5 cut(s)	314, 323, 337, 558, 1129
BstNI	CCWGG	1 cut(s)	229
BstSCI	CCNGG	2 cut(s)	227, 358
BstSFI	CTRYAG	1 cut(s)	97
BstSLI	GKGCMC	1 cut(s)	415
BstV1I	GCAGC	2 cut(s)	318, 749
BstV2I	GAAGAC	1 cut(s)	1008
BstX2I	RGATCY	2 cut(s)	383, 710
BstXI	CCANNNNNNTGG	1 cut(s)	263
BstYI	RGATCY	2 cut(s)	383, 710
Bsu15I	ATCGAT	1 cut(s)	892
BsuRI	GGCC	5 cut(s)	35, 232, 413, 1035, 1105
BsuTUI	ATCGAT	1 cut(s)	892
BtgZI	GCGATG	1 cut(s)	291
BtsCI	GGATG	3 cut(s)	256, 940, 944
BtsIMutI	CAGTG	1 cut(s)	651
BveI	ACCTGC	1 cut(s)	736
Cac8I	GCNNGC	1 cut(s)	37
CaiI	CAGNNNCTG	2 cut(s)	437, 499
CfoI	GCGC	1 cut(s)	139
Cfr13I	GGNCC	5 cut(s)	362, 411, 412, 953, 1103
ClaI	ATCGAT	1 cut(s)	892
CseI	GACGC	1 cut(s)	314
Csp6I	GTAC	5 cut(s)	94, 105, 541, 948, 958
CspCI	CAANNNNNGTGG	2 cut(s)	333, 368
CviAII	CATG	2 cut(s)	83, 368
CviQI	GTAC	5 cut(s)	94, 105, 541, 948, 958
DdeI	CTNAG	6 cut(s)	11, 318, 527, 676, 779, 885
DpnI	GATC	5 cut(s)	81, 90, 385, 712, 891
DpnII	GATC	5 cut(s)	79, 88, 383, 710, 889
EaeI	YGGCCR	3 cut(s)	33, 230, 1033
Eco24I	GRGCYC	2 cut(s)	415, 609
Eco47I	GGWCC	2 cut(s)	362, 953
Eco57I	CTGAAG	1 cut(s)	777
EcoO109I	RGGNCCY	3 cut(s)	411, 412, 1103
EcoRI	GAATTC	1 cut(s)	522
EcoRII	CCWGG	1 cut(s)	227
EcoT38I	GRGCYC	2 cut(s)	415, 609
FaeI	CATG	2 cut(s)	86, 371
FaqI	GGGAC	2 cut(s)	167, 275
FatI	CATG	2 cut(s)	82, 367
FauI	CCCGC	1 cut(s)	400
FauNDI	CATATG	2 cut(s)	235, 913
FbaI	TGATCA	1 cut(s)	79
Fnu4HI	GCNGC	2 cut(s)	332, 763
FokI	GGATG	3 cut(s)	243, 947, 951
FriOI	GRGCYC	2 cut(s)	415, 609
Fsp4HI	GCNGC	2 cut(s)	332, 763
FspBI	CTAG	2 cut(s)	108, 809
GlaI	GCGC	1 cut(s)	138
GluI	GCNGC	2 cut(s)	332, 763
GsaI	CCCAGC	1 cut(s)	613
HaeII	RGCGCY	1 cut(s)	140
HaeIII	GGCC	5 cut(s)	35, 232, 413, 1035, 1105
HapII	CCGG	1 cut(s)	360
HgaI	GACGC	1 cut(s)	314
HhaI	GCGC	1 cut(s)	139
Hin1II	CATG	2 cut(s)	86, 371
Hin6I	GCGC	1 cut(s)	137
HinP1I	GCGC	1 cut(s)	137
HinfI	GANTC	3 cut(s)	155, 270, 849
HpaII	CCGG	1 cut(s)	360
HphI	GGTGA	1 cut(s)	150
Hpy166II	GTNNAC	4 cut(s)	25, 365, 541, 921
Hpy188I	TCNGA	5 cut(s)	528, 557, 702, 780, 934
Hpy188III	TCNNGA	3 cut(s)	77, 634, 846
Hpy8I	GTNNAC	4 cut(s)	25, 365, 541, 921
Hpy99I	CGWCG	1 cut(s)	308
HpyAV	CCTTC	5 cut(s)	67, 71, 678, 752, 764
HpyCH4III	ACNGT	6 cut(s)	71, 101, 205, 655, 957, 987
HpyCH4IV	ACGT	1 cut(s)	27
HpyF10VI	GCNNNNNNNGC	5 cut(s)	314, 323, 337, 558, 1129
HpyF3I	CTNAG	6 cut(s)	11, 318, 527, 676, 779, 885
HpySE526I	ACGT	1 cut(s)	27
Hsp92II	CATG	2 cut(s)	86, 371
HspAI	GCGC	1 cut(s)	137
Ksp22I	TGATCA	1 cut(s)	79
Kzo9I	GATC	5 cut(s)	79, 88, 383, 710, 889
LmnI	GCTCC	1 cut(s)	404
Lsp1109I	GCAGC	2 cut(s)	318, 749
LweI	GCATC	4 cut(s)	327, 561, 868, 929
MaeI	CTAG	2 cut(s)	108, 809
MaeII	ACGT	1 cut(s)	27
MaeIII	GTNAC	4 cut(s)	199, 475, 641, 751
MalI	GATC	5 cut(s)	81, 90, 385, 712, 891
MbiI	CCGCTC	1 cut(s)	407
MboI	GATC	5 cut(s)	79, 88, 383, 710, 889
MboII	GAAGA	4 cut(s)	221, 241, 279, 1013
MflI	RGATCY	2 cut(s)	383, 710
MhlI	GDGCHC	2 cut(s)	415, 609
MlsI	TGGCCA	3 cut(s)	35, 232, 1035
MluCI	AATT	5 cut(s)	51, 189, 522, 690, 992
MluNI	TGGCCA	3 cut(s)	35, 232, 1035
MlyI	GAGTC	1 cut(s)	149
MmeI	TCCRAC	2 cut(s)	411, 912
MnlI	CCTC	6 cut(s)	160, 551, 610, 674, 830, 880
Mox20I	TGGCCA	3 cut(s)	35, 232, 1035
MroXI	GAANNNNTTC	1 cut(s)	774
MscI	TGGCCA	3 cut(s)	35, 232, 1035
MseI	TTAA	2 cut(s)	867, 900
Msp20I	TGGCCA	3 cut(s)	35, 232, 1035
MspI	CCGG	1 cut(s)	360
MspR9I	CCNGG	2 cut(s)	229, 360
MvaI	CCWGG	1 cut(s)	229
MwoI	GCNNNNNNNGC	5 cut(s)	314, 323, 337, 558, 1129
NciI	CCSGG	1 cut(s)	360
NdeI	CATATG	2 cut(s)	235, 913
NdeII	GATC	5 cut(s)	79, 88, 383, 710, 889
NlaIII	CATG	2 cut(s)	86, 371
NlaIV	GGNNCC	5 cut(s)	385, 412, 413, 722, 1087
NmuCI	GTSAC	1 cut(s)	475
PdmI	GAANNNNTTC	1 cut(s)	774
PfeI	GAWTC	2 cut(s)	270, 849
PkrI	GCNGC	2 cut(s)	333, 764
PleI	GAGTC	1 cut(s)	149
PpsI	GAGTC	1 cut(s)	149
Ppu21I	YACGTR	1 cut(s)	28
Psp6I	CCWGG	1 cut(s)	227
PspFI	CCCAGC	1 cut(s)	609
PspGI	CCWGG	1 cut(s)	227
PspN4I	GGNNCC	5 cut(s)	385, 412, 413, 722, 1087
PspOMI	GGGCCC	1 cut(s)	411
PspPI	GGNCC	5 cut(s)	362, 411, 412, 953, 1103
PsrI	GAACNNNNNNTAC	2 cut(s)	1078, 1110
PstNI	CAGNNNCTG	2 cut(s)	437, 499
PsuI	RGATCY	2 cut(s)	383, 710
RsaI	GTAC	5 cut(s)	95, 106, 542, 949, 959
RsaNI	GTAC	5 cut(s)	94, 105, 541, 948, 958
SaqAI	TTAA	2 cut(s)	867, 900
SatI	GCNGC	2 cut(s)	332, 763
Sau3AI	GATC	5 cut(s)	79, 88, 383, 710, 889
Sau96I	GGNCC	5 cut(s)	362, 411, 412, 953, 1103
ScaI	AGTACT	2 cut(s)	95, 106
SchI	GAGTC	1 cut(s)	149
ScrFI	CCNGG	2 cut(s)	229, 360
SduI	GDGCHC	2 cut(s)	415, 609
SfaNI	GCATC	4 cut(s)	327, 561, 868, 929
SfcI	CTRYAG	1 cut(s)	97
SinI	GGWCC	2 cut(s)	362, 953
SpeI	ACTAGT	1 cut(s)	107
Sse9I	AATT	5 cut(s)	51, 189, 522, 690, 992
SsiI	CCGC	1 cut(s)	407
SspI	AATATT	3 cut(s)	744, 857, 864
SspMI	CTAG	2 cut(s)	108, 809
StyD4I	CCNGG	2 cut(s)	227, 358
TaaI	ACNGT	6 cut(s)	71, 101, 205, 655, 957, 987
TaiI	ACGT	1 cut(s)	30
TaqI	TCGA	3 cut(s)	303, 892, 1040
TaqII	GACCGA	1 cut(s)	617
TasI	AATT	5 cut(s)	51, 189, 522, 690, 992
TatI	WGTACW	3 cut(s)	93, 104, 540
TfiI	GAWTC	2 cut(s)	270, 849
Tru1I	TTAA	2 cut(s)	867, 900
Tru9I	TTAA	2 cut(s)	867, 900
TscAI	CASTG	1 cut(s)	658
TseFI	GTSAC	1 cut(s)	475
TseI	GCWGC	2 cut(s)	331, 762
Tsp45I	GTSAC	1 cut(s)	475
TspDTI	ATGAA	1 cut(s)	231
TspRI	CASTG	1 cut(s)	658
VpaK11BI	GGWCC	2 cut(s)	362, 953
XapI	RAATTY	1 cut(s)	522
XcmI	CCANNNNNNNNNTGG	2 cut(s)	260, 1017
XmnI	GAANNNNTTC	1 cut(s)	774
XspI	CTAG	2 cut(s)	108, 809
ZrmI	AGTACT	2 cut(s)	95, 106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.