Belongs to the 'GDSL' lipolytic enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 116 |
| AcuI | CTGAAG | 1 cut(s) | 51 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 14 |
| AgsI | TTSAA | 4 cut(s) | 85, 115, 171, 184 |
| AjnI | CCWGG | 1 cut(s) | 106 |
| AluBI | AGCT | 5 cut(s) | 68, 102, 308, 341, 406 |
| AluI | AGCT | 5 cut(s) | 68, 102, 308, 341, 406 |
| Alw21I | GWGCWC | 2 cut(s) | 70, 310 |
| AoxI | GGCC | 1 cut(s) | 377 |
| ApeKI | GCWGC | 2 cut(s) | 197, 273 |
| ApoI | RAATTY | 1 cut(s) | 116 |
| AspS9I | GGNCC | 2 cut(s) | 359, 377 |
| AvaII | GGWCC | 1 cut(s) | 359 |
| BanII | GRGCYC | 2 cut(s) | 70, 310 |
| Bbv12I | GWGCWC | 2 cut(s) | 70, 310 |
| BbvI | GCAGC | 2 cut(s) | 184, 285 |
| BccI | CCATC | 2 cut(s) | 182, 296 |
| BciT130I | CCWGG | 1 cut(s) | 108 |
| BfaI | CTAG | 2 cut(s) | 47, 338 |
| BisI | GCNGC | 2 cut(s) | 198, 274 |
| BlsI | GCNGC | 2 cut(s) | 199, 275 |
| Bme1390I | CCNGG | 1 cut(s) | 108 |
| Bme18I | GGWCC | 1 cut(s) | 359 |
| BmgT120I | GGNCC | 2 cut(s) | 359, 377 |
| BmrFI | CCNGG | 1 cut(s) | 108 |
| BmrI | ACTGGG | 1 cut(s) | 276 |
| BmuI | ACTGGG | 1 cut(s) | 276 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 14 |
| Bse1I | ACTGG | 1 cut(s) | 282 |
| Bse3DI | GCAATG | 1 cut(s) | 75 |
| BseBI | CCWGG | 1 cut(s) | 108 |
| BseGI | GGATG | 1 cut(s) | 193 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 14 |
| BseMI | GCAATG | 1 cut(s) | 75 |
| BseNI | ACTGG | 1 cut(s) | 282 |
| BseXI | GCAGC | 2 cut(s) | 184, 285 |
| BshFI | GGCC | 1 cut(s) | 379 |
| BsiHKAI | GWGCWC | 2 cut(s) | 70, 310 |
| BslFI | GGGAC | 2 cut(s) | 164, 268 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 14 |
| BsmFI | GGGAC | 2 cut(s) | 164, 268 |
| BsnI | GGCC | 1 cut(s) | 379 |
| Bsp1286I | GDGCHC | 2 cut(s) | 70, 310 |
| BspANI | GGCC | 1 cut(s) | 379 |
| BspQI | GCTCTTC | 1 cut(s) | 92 |
| BsrDI | GCAATG | 1 cut(s) | 75 |
| BsrI | ACTGG | 1 cut(s) | 282 |
| Bst2UI | CCWGG | 1 cut(s) | 108 |
| Bst4CI | ACNGT | 1 cut(s) | 388 |
| Bst6I | CTCTTC | 1 cut(s) | 92 |
| BstC8I | GCNNGC | 1 cut(s) | 408 |
| BstF5I | GGATG | 1 cut(s) | 193 |
| BstNI | CCWGG | 1 cut(s) | 108 |
| BstSCI | CCNGG | 1 cut(s) | 106 |
| BstV1I | GCAGC | 2 cut(s) | 184, 285 |
| BsuRI | GGCC | 1 cut(s) | 379 |
| BtsCI | GGATG | 1 cut(s) | 193 |
| BtsI | GCAGTG | 1 cut(s) | 217 |
| BtsIMutI | CAGTG | 2 cut(s) | 217, 384 |
| Cac8I | GCNNGC | 1 cut(s) | 408 |
| Cfr13I | GGNCC | 2 cut(s) | 359, 377 |
| CviJI | RGCY | 8 cut(s) | 50, 68, 102, 197, 308, 341, 379, 406 |
| CviKI_1 | RGCY | 8 cut(s) | 50, 68, 102, 197, 308, 341, 379, 406 |
| Eam1104I | CTCTTC | 1 cut(s) | 92 |
| EarI | CTCTTC | 1 cut(s) | 92 |
| Ecl136II | GAGCTC | 2 cut(s) | 68, 308 |
| Eco24I | GRGCYC | 2 cut(s) | 70, 310 |
| Eco47I | GGWCC | 1 cut(s) | 359 |
| Eco53kI | GAGCTC | 2 cut(s) | 68, 308 |
| Eco57I | CTGAAG | 1 cut(s) | 51 |
| EcoICRI | GAGCTC | 2 cut(s) | 68, 308 |
| EcoO109I | RGGNCCY | 1 cut(s) | 377 |
| EcoRII | CCWGG | 1 cut(s) | 106 |
| EcoT38I | GRGCYC | 2 cut(s) | 70, 310 |
| FaqI | GGGAC | 2 cut(s) | 164, 268 |
| FauNDI | CATATG | 1 cut(s) | 262 |
| Fnu4HI | GCNGC | 2 cut(s) | 198, 274 |
| FokI | GGATG | 1 cut(s) | 200 |
| FriOI | GRGCYC | 2 cut(s) | 70, 310 |
| Fsp4HI | GCNGC | 2 cut(s) | 198, 274 |
| FspBI | CTAG | 2 cut(s) | 47, 338 |
| GluI | GCNGC | 2 cut(s) | 198, 274 |
| HaeIII | GGCC | 1 cut(s) | 379 |
| HinfI | GANTC | 4 cut(s) | 4, 54, 111, 156 |
| Hpy188I | TCNGA | 1 cut(s) | 31 |
| Hpy188III | TCNNGA | 1 cut(s) | 85 |
| HpyAV | CCTTC | 3 cut(s) | 91, 177, 240 |
| HpyCH4III | ACNGT | 1 cut(s) | 388 |
| HpyCH4V | TGCA | 3 cut(s) | 11, 143, 273 |
| LguI | GCTCTTC | 1 cut(s) | 92 |
| LmnI | GCTCC | 2 cut(s) | 215, 305 |
| LpnPI | CCDG | 6 cut(s) | 21, 64, 93, 120, 295, 360 |
| Lsp1109I | GCAGC | 2 cut(s) | 184, 285 |
| MaeI | CTAG | 2 cut(s) | 47, 338 |
| MboII | GAAGA | 2 cut(s) | 44, 109 |
| MhlI | GDGCHC | 2 cut(s) | 70, 310 |
| MluCI | AATT | 2 cut(s) | 116, 232 |
| MlyI | GAGTC | 1 cut(s) | 48 |
| MseI | TTAA | 2 cut(s) | 41, 412 |
| MspR9I | CCNGG | 1 cut(s) | 108 |
| MvaI | CCWGG | 1 cut(s) | 108 |
| NdeI | CATATG | 1 cut(s) | 262 |
| PciSI | GCTCTTC | 1 cut(s) | 92 |
| PfeI | GAWTC | 3 cut(s) | 4, 111, 156 |
| PkrI | GCNGC | 2 cut(s) | 199, 275 |
| PleI | GAGTC | 1 cut(s) | 48 |
| PpsI | GAGTC | 1 cut(s) | 48 |
| Psp124BI | GAGCTC | 2 cut(s) | 70, 310 |
| Psp6I | CCWGG | 1 cut(s) | 106 |
| PspGI | CCWGG | 1 cut(s) | 106 |
| PspPI | GGNCC | 2 cut(s) | 359, 377 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 10, 42 |
| SacI | GAGCTC | 2 cut(s) | 70, 310 |
| SapI | GCTCTTC | 1 cut(s) | 92 |
| SaqAI | TTAA | 2 cut(s) | 41, 412 |
| SatI | GCNGC | 2 cut(s) | 198, 274 |
| Sau96I | GGNCC | 2 cut(s) | 359, 377 |
| SchI | GAGTC | 1 cut(s) | 48 |
| ScrFI | CCNGG | 1 cut(s) | 108 |
| SduI | GDGCHC | 2 cut(s) | 70, 310 |
| SetI | ASST | 5 cut(s) | 70, 104, 310, 343, 408 |
| SinI | GGWCC | 1 cut(s) | 359 |
| Sse9I | AATT | 2 cut(s) | 116, 232 |
| SspI | AATATT | 1 cut(s) | 126 |
| SspMI | CTAG | 2 cut(s) | 47, 338 |
| SstI | GAGCTC | 2 cut(s) | 70, 310 |
| StyD4I | CCNGG | 1 cut(s) | 106 |
| TaaI | ACNGT | 1 cut(s) | 388 |
| TaqI | TCGA | 1 cut(s) | 129 |
| TasI | AATT | 2 cut(s) | 116, 232 |
| TfiI | GAWTC | 3 cut(s) | 4, 111, 156 |
| Tru1I | TTAA | 2 cut(s) | 41, 412 |
| Tru9I | TTAA | 2 cut(s) | 41, 412 |
| TscAI | CASTG | 2 cut(s) | 217, 391 |
| TseI | GCWGC | 2 cut(s) | 197, 273 |
| TspDTI | ATGAA | 3 cut(s) | 17, 64, 279 |
| TspRI | CASTG | 2 cut(s) | 217, 391 |
| VpaK11BI | GGWCC | 1 cut(s) | 359 |
| XapI | RAATTY | 1 cut(s) | 116 |
| XspI | CTAG | 2 cut(s) | 47, 338 |