Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000443

Physical Location & Seq

Genomic Coordinates Forward (+)

61209375 .. 61221595

Gene Structure 12221 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G037170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1053 bp

ATGAAGAAGACACAGAAGACAGAGGAGCCGGCAGATAATCATGAGCTAAAACAGAAGGCGGCGGCGTTGTTTGTGTTTGGGGACTCACTCTTTGATATTGGCAATAATCAATACCTTAATACTAGCGCACGAGAATTATCGGCAACATACTGGCCATATGGAGAAAACTTCTTCCATCATCCCACCGGGAGATTTTCCGACGGTCGTTTAGTCCCTGATTTCATCCGACAGTTTGCAAAGTTGCCCATCATTCCACCGTACCTGCAACCTGGTCCCCATAATTTTACTGACGGAACCAACTTTGCTTCAGCCGACGCCGGTATTCTTCCTGAAACCAATCCAGGAACAGTAAGTCTTCCATTGCAGCTAAGCTATTTTAAGGCCGCGAAGAAGTTGCTGCAACAACAACTTGGTGATGAAGAGGCCAAGAGTTTGCTGGGGAGAGCTGTTTACTTGATCAGCATTGGTGGCAATGATTATTTTAGCTTCTACGCCAATTACCCCTACGCCAATGAGACCATCCAGCAAGAATTTGTAGCTATTGTGATCAGCAAGTTGACCATTGTGCTCCAATCGGCACCGACGGCGGTGGTACGGCCTGTTTTCGGCTGGTTTCGGCATGTTCGGGAATTTCAGCCACCCCTATACAACACTGAACTTAGTGATGGGTGTGTTGAAGAACTCGAGAGTTTGATAAGGCTACACAATACAGCTCTGGCAAATGTCCTCAAAGAGTTGGAGAGCCAGAAGCCAGGGTTCAAATACTCAGTTTTTGAGTACTATGATGCGATTGGAGATCGAATGCATAGCCCAGCAAAATATGGCTTTACTGACGGCTCAGATGCGTGTTGTGGTATTGGAGCATACTGGGGATCTGGTTGCGGTAATGAAACAAAAGGGTATGAGTTATGTAGTGACCCTGGGAATTATGTGTGGTTCGATGGTAGCCATACCACTGAAAAAGTCAACCTACAATTAGCAAAGCTGATATGGAGTGGAACACCAGATGTAACTGGGCCTTACAATGTTAAACAACTTTTTGGACATGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005840 GO:0005911 GO:0006082 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009409 GO:0009505 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009941 GO:0009987 GO:0016020 GO:0016143 GO:0016145 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0022626 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032991 GO:0042579 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043436 GO:0044237 GO:0044248 GO:0044273 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_GDSL

Protein Analysis

350

Amino Acids

39.02

Weight (kDa)

5.5

Isoelectric Point (pI)

31.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_GDSL	PF00657	23 - 330	5.9e-24	GDSL-like Lipase/Acylhydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000443)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35490 FvH4_6g35490 FvH4_6g35500 FvH4_6g35500 FvH4_6g35500 FvH4_6g35520 FvH4_6g35520 FvH4_6g35540 FvH4_6g35550
malus_domestica	MD09G1175400.v1.1
pyrus_communis	pycom09g09260
rosa_chinensis	RchiOBHm_Chr2g0144821 RchiOBHm_Chr2g0145011 RchiOBHm_Chr2g0145061 RchiOBHm_Chr2g0147051 RchiOBHm_Chr2g0147071 RchiOBHm_Chr2g0147111 RchiOBHm_Chr2g0147141
rosa_laevigata	RLG00000020116 RLG00000020119 RLG00000020120 RLG00000020122 RLG00000020243 RLG00000020245 RLG00000020246
rosa_multiflora	Rmu_co8407447.1_g000001 Rmu_sc0000070.1_g000059 Rmu_sc0001668.1_g000005 Rmu_sc0001880.1_g000013 Rmu_sc0001880.1_g000020 Rmu_sc0001880.1_g000023 Rmu_sc0001880.1_g000027 Rmu_sc0002354.1_g000023 Rmu_sc0002506.1_g000001 Rmu_sc0002506.1_g000016 Rmu_sc0004897.1_g000008 Rmu_sc0007988.1_g000001
rosa_roxburghii	Rroxscaffold_1G00033230 Rroxscaffold_2G00099670 Rroxscaffold_2G00099720 Rroxscaffold_2G00100900 Rroxscaffold_2G00100930 Rroxscaffold_2G00100940
rosa_rugosa	Rorug02G0390000 Rorug02G0390100 Rorug02G0390200 Rorug02G0390200 Rorug02G0390200 Rorug02G0398400 Rorug02G0398500 Rorug02G0398600
rosa_samantha	Rh2AG442200 Rh2AG442500 Rh2AG442700 Rh2AG455600 Rh2AG455700 Rh2AG455800 Rh2AG455900 Rh2BG452500 Rh2BG453000 Rh2BG453700 Rh2BG467900 Rh2BG468100 Rh2BG468200 Rh2CG429200 Rh2CG429500 Rh2CG429600 Rh2CG429700 Rh2CG443000 Rh2CG443100 Rh2CG443200 Rh2CG443300 Rh2DG462200 Rh2DG463000 Rh2DG463200 Rh2DG463700 Rh2DG477300 Rh2DG477500 Rh2DG477600 Rh4AG390700 Rh5AG320200
rosa_wichuraiana	Rw2G036130 Rw2G036170 Rw2G036180 Rw2G037150 Rw2G037160 Rw2G037170 Rw2G037180 Rw2G037260 Rw2G037270 Rw2G037280 Rw2G037290 Rw5G030070 Rw5G030220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	270
AccB1I	GGYRCC	1 cut(s)	577
AccII	CGCG	1 cut(s)	386
AciI	CCGC	5 cut(s)	59, 62, 384, 587, 882
AclWI	GGATC	1 cut(s)	880
AcoI	YGGCCR	1 cut(s)	152
AcsI	RAATTY	2 cut(s)	530, 629
AcuI	CTGAAG	1 cut(s)	291
AcyI	GRCGYC	1 cut(s)	315
AfaI	GTAC	3 cut(s)	260, 594, 779
AfiI	CCNNNNNNNGG	1 cut(s)	605
AflIII	ACRYGT	1 cut(s)	1045
AgsI	TTSAA	2 cut(s)	677, 760
AjnI	CCWGG	4 cut(s)	268, 340, 751, 919
AluBI	AGCT	8 cut(s)	46, 367, 372, 446, 486, 539, 713, 985
AluI	AGCT	8 cut(s)	46, 367, 372, 446, 486, 539, 713, 985
Alw21I	GWGCWC	1 cut(s)	570
Alw26I	GTCTC	1 cut(s)	509
AlwI	GGATC	1 cut(s)	880
Ama87I	CYCGRG	1 cut(s)	683
AoxI	GGCC	5 cut(s)	152, 381, 423, 596, 1016
ApeKI	GCWGC	2 cut(s)	364, 397
ApoI	RAATTY	2 cut(s)	530, 629
ArsI	GACNNNNNNTTYG	2 cut(s)	74, 106
AspLEI	GCGC	1 cut(s)	128
AspS9I	GGNCC	2 cut(s)	272, 1016
AsuC2I	CCSGG	1 cut(s)	187
AsuHPI	GGTGA	1 cut(s)	425
AvaI	CYCGRG	1 cut(s)	683
AvaII	GGWCC	1 cut(s)	272
BalI	TGGCCA	1 cut(s)	154
BanI	GGYRCC	1 cut(s)	577
BauI	CACGAG	1 cut(s)	129
BbsI	GAAGAC	3 cut(s)	14, 23, 347
Bbv12I	GWGCWC	1 cut(s)	570
BbvI	GCAGC	2 cut(s)	376, 384
BccI	CCATC	5 cut(s)	183, 254, 527, 659, 935
BceAI	ACGGC	3 cut(s)	600, 611, 850
BcgI	CGANNNNNNTGC	2 cut(s)	376, 410
BciT130I	CCWGG	4 cut(s)	270, 342, 753, 921
BclI	TGATCA	2 cut(s)	456, 546
BcnI	CCSGG	1 cut(s)	187
BcoDI	GTCTC	1 cut(s)	509
BfaI	CTAG	1 cut(s)	123
BfuAI	ACCTGC	1 cut(s)	270
BisI	GCNGC	5 cut(s)	60, 63, 365, 384, 398
BlpI	GCTNAGC	1 cut(s)	368
BlsI	GCNGC	5 cut(s)	61, 64, 366, 385, 399
BmcAI	AGTACT	1 cut(s)	779
Bme1390I	CCNGG	5 cut(s)	187, 270, 342, 753, 921
Bme18I	GGWCC	1 cut(s)	272
BmeT110I	CYCGRG	1 cut(s)	683
BmgT120I	GGNCC	2 cut(s)	272, 1016
BmiI	GGNNCC	4 cut(s)	27, 274, 295, 579
BmrFI	CCNGG	5 cut(s)	187, 270, 342, 753, 921
BmrI	ACTGGG	2 cut(s)	877, 1023
BmsI	GCATC	2 cut(s)	775, 832
BmuI	ACTGGG	2 cut(s)	877, 1023
BpiI	GAAGAC	3 cut(s)	14, 23, 347
Bpu1102I	GCTNAGC	1 cut(s)	368
BpuMI	CCSGG	1 cut(s)	187
BsaHI	GRCGYC	1 cut(s)	315
BsaI	GGTCTC	1 cut(s)	509
BsaJI	CCNNGG	3 cut(s)	752, 919, 920
Bsc4I	CCNNNNNNNGG	1 cut(s)	605
Bse118I	RCCGGY	2 cut(s)	28, 317
Bse1I	ACTGG	3 cut(s)	155, 872, 1018
Bse3DI	GCAATG	2 cut(s)	359, 478
BseBI	CCWGG	4 cut(s)	270, 342, 753, 921
BseDI	CCNNGG	3 cut(s)	752, 919, 920
BseGI	GGATG	3 cut(s)	178, 222, 519
BseLI	CCNNNNNNNGG	1 cut(s)	605
BseMI	GCAATG	2 cut(s)	359, 478
BseMII	CTCAG	2 cut(s)	780, 852
BseNI	ACTGG	3 cut(s)	155, 872, 1018
BseRI	GAGGAG	1 cut(s)	38
BseXI	GCAGC	2 cut(s)	376, 384
BseYI	CCCAGC	2 cut(s)	436, 811
Bsh1236I	CGCG	1 cut(s)	386
Bsh1285I	CGRYCG	1 cut(s)	205
BshFI	GGCC	5 cut(s)	154, 383, 425, 598, 1018
BshNI	GGYRCC	1 cut(s)	577
BsiEI	CGRYCG	1 cut(s)	205
BsiHKAI	GWGCWC	1 cut(s)	570
BsiHKCI	CYCGRG	1 cut(s)	683
BsiSI	CCGG	3 cut(s)	29, 186, 318
BslFI	GGGAC	3 cut(s)	95, 197, 258
BslI	CCNNNNNNNGG	1 cut(s)	605
BsmAI	GTCTC	1 cut(s)	509
BsmFI	GGGAC	3 cut(s)	95, 197, 258
BsmI	GAATGC	1 cut(s)	807
BsnI	GGCC	5 cut(s)	154, 383, 425, 598, 1018
Bso31I	GGTCTC	1 cut(s)	509
BsoBI	CYCGRG	1 cut(s)	683
Bsp1286I	GDGCHC	1 cut(s)	570
Bsp143I	GATC	4 cut(s)	456, 546, 796, 872
Bsp1720I	GCTNAGC	1 cut(s)	368
BspACI	CCGC	5 cut(s)	59, 62, 384, 587, 882
BspANI	GGCC	5 cut(s)	154, 383, 425, 598, 1018
BspCNI	CTCAG	2 cut(s)	779, 851
BspFNI	CGCG	1 cut(s)	386
BspHI	TCATGA	1 cut(s)	40
BspLI	GGNNCC	4 cut(s)	27, 274, 295, 579
BspMI	ACCTGC	1 cut(s)	270
BspPI	GGATC	1 cut(s)	880
BspT107I	GGYRCC	1 cut(s)	577
BspTNI	GGTCTC	1 cut(s)	509
BsrDI	GCAATG	2 cut(s)	359, 478
BsrFI	RCCGGY	2 cut(s)	28, 317
BsrI	ACTGG	3 cut(s)	155, 872, 1018
BssAI	RCCGGY	2 cut(s)	28, 317
BssECI	CCNNGG	3 cut(s)	752, 919, 920
BssMI	GATC	4 cut(s)	456, 546, 796, 872
BssNI	GRCGYC	1 cut(s)	315
BssSI	CACGAG	1 cut(s)	129
Bst2BI	CACGAG	1 cut(s)	129
Bst2UI	CCWGG	4 cut(s)	270, 342, 753, 921
Bst4CI	ACNGT	4 cut(s)	203, 231, 258, 349
Bst6I	CTCTTC	1 cut(s)	414
BstACI	GRCGYC	1 cut(s)	315
BstC8I	GCNNGC	1 cut(s)	30
BstDEI	CTNAG	4 cut(s)	368, 659, 766, 838
BstF5I	GGATG	3 cut(s)	178, 222, 519
BstFNI	CGCG	1 cut(s)	386
BstHHI	GCGC	1 cut(s)	128
BstKTI	GATC	4 cut(s)	459, 549, 799, 875
BstMAI	GTCTC	1 cut(s)	509
BstMBI	GATC	4 cut(s)	456, 546, 796, 872
BstMCI	CGRYCG	1 cut(s)	205
BstMWI	GCNNNNNNNGC	2 cut(s)	468, 584
BstNI	CCWGG	4 cut(s)	270, 342, 753, 921
BstNSI	RCATGY	2 cut(s)	623, 1049
BstSCI	CCNGG	5 cut(s)	185, 268, 340, 751, 919
BstUI	CGCG	1 cut(s)	386
BstV1I	GCAGC	2 cut(s)	376, 384
BstV2I	GAAGAC	3 cut(s)	14, 23, 347
BstX2I	RGATCY	1 cut(s)	872
BstYI	RGATCY	1 cut(s)	872
BsuRI	GGCC	5 cut(s)	154, 383, 425, 598, 1018
BtsCI	GGATG	3 cut(s)	178, 222, 519
BtsIMutI	CAGTG	2 cut(s)	651, 954
BveI	ACCTGC	1 cut(s)	270
Cac8I	GCNNGC	1 cut(s)	30
CciI	TCATGA	1 cut(s)	40
CfoI	GCGC	1 cut(s)	128
Cfr10I	RCCGGY	2 cut(s)	28, 317
Cfr13I	GGNCC	2 cut(s)	272, 1016
CseI	GACGC	1 cut(s)	323
CsiI	ACCWGGT	1 cut(s)	268
Csp6I	GTAC	3 cut(s)	259, 593, 778
CviAII	CATG	3 cut(s)	41, 620, 1046
CviQI	GTAC	3 cut(s)	259, 593, 778
DdeI	CTNAG	4 cut(s)	368, 659, 766, 838
DpnI	GATC	4 cut(s)	458, 548, 798, 874
DpnII	GATC	4 cut(s)	456, 546, 796, 872
EaeI	YGGCCR	1 cut(s)	152
Eam1104I	CTCTTC	1 cut(s)	414
EarI	CTCTTC	1 cut(s)	414
Eco31I	GGTCTC	1 cut(s)	509
Eco47I	GGWCC	1 cut(s)	272
Eco57I	CTGAAG	1 cut(s)	291
Eco88I	CYCGRG	1 cut(s)	683
EcoRII	CCWGG	4 cut(s)	268, 340, 751, 919
EcoT22I	ATGCAT	1 cut(s)	807
FaeI	CATG	3 cut(s)	44, 623, 1049
FaqI	GGGAC	3 cut(s)	95, 197, 258
FatI	CATG	3 cut(s)	40, 619, 1045
FauNDI	CATATG	1 cut(s)	157
FbaI	TGATCA	2 cut(s)	456, 546
Fnu4HI	GCNGC	5 cut(s)	60, 63, 365, 384, 398
FokI	GGATG	3 cut(s)	165, 209, 506
Fsp4HI	GCNGC	5 cut(s)	60, 63, 365, 384, 398
FspBI	CTAG	1 cut(s)	123
GlaI	GCGC	1 cut(s)	127
GluI	GCNGC	5 cut(s)	60, 63, 365, 384, 398
GsaI	CCCAGC	2 cut(s)	440, 815
HaeIII	GGCC	5 cut(s)	154, 383, 425, 598, 1018
HapII	CCGG	3 cut(s)	29, 186, 318
HgaI	GACGC	1 cut(s)	323
HhaI	GCGC	1 cut(s)	128
Hin1I	GRCGYC	1 cut(s)	315
Hin1II	CATG	3 cut(s)	44, 623, 1049
Hin6I	GCGC	1 cut(s)	126
HinP1I	GCGC	1 cut(s)	126
HincII	GTYRAC	2 cut(s)	558, 967
HindII	GTYRAC	2 cut(s)	558, 967
HinfI	GANTC	1 cut(s)	83
HpaII	CCGG	3 cut(s)	29, 186, 318
HphI	GGTGA	1 cut(s)	425
Hpy166II	GTNNAC	3 cut(s)	451, 558, 967
Hpy188I	TCNGA	3 cut(s)	199, 227, 841
Hpy188III	TCNNGA	4 cut(s)	41, 329, 626, 685
Hpy8I	GTNNAC	3 cut(s)	451, 558, 967
Hpy99I	CGWCG	3 cut(s)	203, 317, 586
HpyAV	CCTTC	1 cut(s)	49
HpyCH4III	ACNGT	4 cut(s)	203, 231, 258, 349
HpyCH4V	TGCA	5 cut(s)	236, 265, 364, 400, 805
HpyF10VI	GCNNNNNNNGC	2 cut(s)	468, 584
HpyF3I	CTNAG	4 cut(s)	368, 659, 766, 838
Hsp92I	GRCGYC	1 cut(s)	315
Hsp92II	CATG	3 cut(s)	44, 623, 1049
HspAI	GCGC	1 cut(s)	126
KroI	GCCGGC	1 cut(s)	28
KroNI	GCCGGC	1 cut(s)	30
Ksp22I	TGATCA	2 cut(s)	456, 546
Kzo9I	GATC	4 cut(s)	456, 546, 796, 872
LmnI	GCTCC	3 cut(s)	25, 573, 860
Lsp1109I	GCAGC	2 cut(s)	376, 384
LweI	GCATC	2 cut(s)	775, 832
MabI	ACCWGGT	1 cut(s)	268
MaeI	CTAG	1 cut(s)	123
MaeIII	GTNAC	2 cut(s)	914, 1009
MalI	GATC	4 cut(s)	458, 548, 798, 874
MboI	GATC	4 cut(s)	456, 546, 796, 872
MboII	GAAGA	9 cut(s)	16, 19, 28, 163, 317, 347, 400, 431, 689
MflI	RGATCY	1 cut(s)	872
MhlI	GDGCHC	1 cut(s)	570
MlsI	TGGCCA	1 cut(s)	154
MluCI	AATT	7 cut(s)	134, 280, 496, 530, 629, 925, 974
MluNI	TGGCCA	1 cut(s)	154
MlyI	GAGTC	1 cut(s)	77
MmeI	TCCRAC	3 cut(s)	222, 250, 717
MnlI	CCTC	3 cut(s)	16, 415, 737
Mox20I	TGGCCA	1 cut(s)	154
Mph1103I	ATGCAT	1 cut(s)	807
MroNI	GCCGGC	1 cut(s)	28
MscI	TGGCCA	1 cut(s)	154
MseI	TTAA	3 cut(s)	117, 378, 1029
Msp20I	TGGCCA	1 cut(s)	154
MspI	CCGG	3 cut(s)	29, 186, 318
MspR9I	CCNGG	5 cut(s)	187, 270, 342, 753, 921
Mva1269I	GAATGC	1 cut(s)	807
MvaI	CCWGG	4 cut(s)	270, 342, 753, 921
MvnI	CGCG	1 cut(s)	386
MwoI	GCNNNNNNNGC	2 cut(s)	468, 584
NaeI	GCCGGC	1 cut(s)	30
NciI	CCSGG	1 cut(s)	187
NdeI	CATATG	1 cut(s)	157
NdeII	GATC	4 cut(s)	456, 546, 796, 872
NgoMIV	GCCGGC	1 cut(s)	28
NlaIII	CATG	3 cut(s)	44, 623, 1049
NlaIV	GGNNCC	4 cut(s)	27, 274, 295, 579
NmuCI	GTSAC	1 cut(s)	914
NsiI	ATGCAT	1 cut(s)	807
NspI	RCATGY	2 cut(s)	623, 1049
PaeR7I	CTCGAG	1 cut(s)	683
PagI	TCATGA	1 cut(s)	40
PasI	CCCWGGG	1 cut(s)	920
PciI	ACATGT	1 cut(s)	1045
PctI	GAATGC	1 cut(s)	807
PdiI	GCCGGC	1 cut(s)	30
PfoI	TCCNGGA	1 cut(s)	340
PkrI	GCNGC	5 cut(s)	61, 64, 366, 385, 399
PleI	GAGTC	1 cut(s)	77
PpsI	GAGTC	1 cut(s)	77
PscI	ACATGT	1 cut(s)	1045
Psp6I	CCWGG	4 cut(s)	268, 340, 751, 919
PspFI	CCCAGC	2 cut(s)	436, 811
PspGI	CCWGG	4 cut(s)	268, 340, 751, 919
PspN4I	GGNNCC	4 cut(s)	27, 274, 295, 579
PspPI	GGNCC	2 cut(s)	272, 1016
PsuI	RGATCY	1 cut(s)	872
RsaI	GTAC	3 cut(s)	260, 594, 779
RsaNI	GTAC	3 cut(s)	259, 593, 778
SaqAI	TTAA	3 cut(s)	117, 378, 1029
SatI	GCNGC	5 cut(s)	60, 63, 365, 384, 398
Sau3AI	GATC	4 cut(s)	456, 546, 796, 872
Sau96I	GGNCC	2 cut(s)	272, 1016
ScaI	AGTACT	1 cut(s)	779
SchI	GAGTC	1 cut(s)	77
ScrFI	CCNGG	5 cut(s)	187, 270, 342, 753, 921
SduI	GDGCHC	1 cut(s)	570
SexAI	ACCWGGT	1 cut(s)	268
SfaNI	GCATC	2 cut(s)	775, 832
Sfr274I	CTCGAG	1 cut(s)	683
SinI	GGWCC	1 cut(s)	272
SlaI	CTCGAG	1 cut(s)	683
SmlI	CTYRAG	1 cut(s)	683
SmoI	CTYRAG	1 cut(s)	683
Sse9I	AATT	7 cut(s)	134, 280, 496, 530, 629, 925, 974
SsiI	CCGC	5 cut(s)	59, 62, 384, 587, 882
SspMI	CTAG	1 cut(s)	123
StyD4I	CCNGG	5 cut(s)	185, 268, 340, 751, 919
TaaI	ACNGT	4 cut(s)	203, 231, 258, 349
TaqI	TCGA	3 cut(s)	684, 799, 939
TasI	AATT	7 cut(s)	134, 280, 496, 530, 629, 925, 974
TatI	WGTACW	1 cut(s)	777
TauI	GCSGC	3 cut(s)	62, 65, 386
Tru1I	TTAA	3 cut(s)	117, 378, 1029
Tru9I	TTAA	3 cut(s)	117, 378, 1029
TscAI	CASTG	2 cut(s)	658, 961
TseFI	GTSAC	1 cut(s)	914
TseI	GCWGC	2 cut(s)	364, 397
Tsp45I	GTSAC	1 cut(s)	914
TspDTI	ATGAA	4 cut(s)	17, 211, 432, 903
TspGWI	ACGGA	1 cut(s)	306
TspRI	CASTG	2 cut(s)	658, 961
VpaK11BI	GGWCC	1 cut(s)	272
XapI	RAATTY	2 cut(s)	530, 629
XceI	RCATGY	2 cut(s)	623, 1049
XcmI	CCANNNNNNNNNTGG	1 cut(s)	433
XhoI	CTCGAG	1 cut(s)	683
XspI	CTAG	1 cut(s)	123
ZrmI	AGTACT	1 cut(s)	779
Zsp2I	ATGCAT	1 cut(s)	807

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.