FvH4_7g01520

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000709
Physical Location & Seq
Reverse (-)
1924234 .. 1928126
3893 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01520.t3

Sequence Viewer

Length: 987 bp
ATGAGCGGAGCAAAGAACAATGTTGTGTGTGTAACAGGAGCTTCTGGTTATATTGCATCATGGCTGGTCAAGCTTTTGTTAGGACGAGGTTATATTGTCAAAGCCACTGTTCGGGACCCAAACGATCTGAAGAAAACAGAACACTTGCTTTCGCTTGATGGAGCAAAAGAAAGGCTTCACTTGTTTAAAGCAGATTTGCTAGATGAAGGATCTTTTGACCATGCGGTTGATGGATGTGATGGTGTTTTCCATACAGCATCTCCGGTACTATTTTCATCCATCAGCGACCCACAGGCAGAACTAATTAACCCTGCTGTGAAGGGAACACTAAATGTTCTGAAGTCATGCAAAAAATTTCCAACTGTCAAGCGAGTGGTTTTGACATCTTCTATAGCTTCTGTGATAATGAATGGAAAACCTCTGACCCCTGATGTTGTAGTGGATGAAACATGGTATTCGAATCCACTTATTGCTGAGAAGAACAAGCGATGGTATCTTCTTTCAAAGACTTTAGCTGAGGAGGCTGCTTGGAAATTTTCTGAAGGAAATGGAATTGACTTGGTTTCAATAAATCCGGGGTTTGTGATTGGACCAATTTTGCAGCCGGCAATTAATTTGACCGTGGAGATGATTCAAAATCTCATAACTGGCAAGGCAGTGCCCGCTTCAAATTACAGATTCATTGATGTTAGAGATGTTGCCTCTGCTCATATTCAAGCATTTGAAGTTCCATCAGCTACAGGAAGGTATTGTTTGGTCAGCCATAGCGCGCACATTTCCGAGGCTCTAGAGATTATAAGAGAGCTTTATCCCAATTTGCACATTCCTGAAAGATGTGAGGATGGTCCTCTTTTGCTCCCTTCTGATCCACAATTTCAAGTATCCAAGGAGAAAGCAAAAGGTCTGGGAATTGATTTTTATCCGTTTGAAGTAACTCTGCAGGACACTATCGAAAGTCTGAAGGAGAAGGGCTTCCTCAACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

329

Amino Acids

35.94

Weight (kDa)

5.93

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 19 - 210 2.3e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 21 - 205 9.6e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 31 - 165 7.9e-09 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 797
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 1 cut(s) 770
AciI CCGC 3 cut(s) 6, 224, 663
AclWI GGATC 2 cut(s) 217, 860
AcsI RAATTY 2 cut(s) 353, 533
AcuI CTGAAG 4 cut(s) 149, 359, 561, 980
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 111
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 8 cut(s) 504, 567, 635, 669, 716, 725, 878, 929
AluBI AGCT 6 cut(s) 41, 73, 395, 515, 737, 805
AluI AGCT 6 cut(s) 41, 73, 395, 515, 737, 805
AlwI GGATC 2 cut(s) 217, 860
ApeKI GCWGC 2 cut(s) 524, 601
ApoI RAATTY 2 cut(s) 353, 533
AseI ATTAAT 1 cut(s) 612
Asp700I GAANNNNTTC 2 cut(s) 174, 971
AspLEI GCGC 2 cut(s) 770, 772
AspS9I GGNCC 3 cut(s) 115, 590, 845
AsuC2I CCSGG 1 cut(s) 576
AsuII TTCGAA 1 cut(s) 458
AvaII GGWCC 3 cut(s) 115, 590, 845
BaeGI GKGCMC 1 cut(s) 663
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 511, 613
BccI CCATC 7 cut(s) 152, 224, 233, 287, 483, 739, 836
BciVI GTATCC 1 cut(s) 892
BcnI CCSGG 1 cut(s) 576
BfaI CTAG 2 cut(s) 200, 788
BfmI CTRYAG 3 cut(s) 390, 738, 938
BfuI GTATCC 1 cut(s) 892
BisI GCNGC 2 cut(s) 525, 602
BlsI GCNGC 2 cut(s) 526, 603
Bme1390I CCNGG 1 cut(s) 576
Bme18I GGWCC 3 cut(s) 115, 590, 845
BmgT120I GGNCC 3 cut(s) 115, 590, 845
BmiI GGNNCC 2 cut(s) 116, 117
BmrFI CCNGG 1 cut(s) 576
BmsI GCATC 2 cut(s) 65, 266
Bpu10I CCTNAGC 1 cut(s) 516
Bpu14I TTCGAA 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 576
BsaBI GATNNNNATC 1 cut(s) 918
BsaJI CCNNGG 4 cut(s) 575, 621, 780, 885
BsaWI WCCGGW 1 cut(s) 262
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse118I RCCGGY 1 cut(s) 604
Bse1I ACTGG 1 cut(s) 652
Bse8I GATNNNNATC 1 cut(s) 918
BseDI CCNNGG 4 cut(s) 575, 621, 780, 885
BseGI GGATG 4 cut(s) 239, 275, 448, 847
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 465, 507
BseNI ACTGG 1 cut(s) 652
BsePI GCGCGC 1 cut(s) 768
BseRI GAGGAG 1 cut(s) 533
BseSI GKGCMC 1 cut(s) 663
BseXI GCAGC 2 cut(s) 511, 613
Bsh1236I CGCG 1 cut(s) 770
BsiSI CCGG 3 cut(s) 263, 575, 605
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 128
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 663
Bsp143I GATC 3 cut(s) 124, 209, 865
BspACI CCGC 3 cut(s) 6, 224, 663
BspCNI CTCAG 2 cut(s) 466, 508
BspFNI CGCG 1 cut(s) 770
BspLI GGNNCC 2 cut(s) 116, 117
BspMAI CTGCAG 1 cut(s) 942
BspPI GGATC 2 cut(s) 217, 860
BspT104I TTCGAA 1 cut(s) 458
BsrBI CCGCTC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 604
BsrI ACTGG 1 cut(s) 652
BssAI RCCGGY 1 cut(s) 604
BssECI CCNNGG 4 cut(s) 575, 621, 780, 885
BssHII GCGCGC 1 cut(s) 768
BssMI GATC 3 cut(s) 124, 209, 865
BssT1I CCWWGG 1 cut(s) 885
Bst4CI ACNGT 3 cut(s) 109, 364, 622
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 3 cut(s) 606, 663, 770
BstDEI CTNAG 2 cut(s) 474, 516
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 4 cut(s) 239, 275, 448, 847
BstFNI CGCG 1 cut(s) 770
BstHHI GCGC 2 cut(s) 770, 772
BstKTI GATC 3 cut(s) 127, 212, 868
BstMBI GATC 3 cut(s) 124, 209, 865
BstMWI GCNNNNNNNGC 3 cut(s) 70, 521, 662
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 1 cut(s) 574
BstSFI CTRYAG 3 cut(s) 390, 738, 938
BstSLI GKGCMC 1 cut(s) 663
BstUI CGCG 1 cut(s) 770
BstV1I GCAGC 2 cut(s) 511, 613
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
BsuI GTATCC 1 cut(s) 892
BtgI CCRYGG 1 cut(s) 621
BtgZI GCGATG 1 cut(s) 502
BtsCI GGATG 4 cut(s) 239, 275, 448, 847
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 2 cut(s) 105, 663
Cac8I GCNNGC 3 cut(s) 606, 663, 770
CfoI GCGC 2 cut(s) 770, 772
Cfr10I RCCGGY 1 cut(s) 604
Cfr13I GGNCC 3 cut(s) 115, 590, 845
Csp6I GTAC 1 cut(s) 266
CviAII CATG 5 cut(s) 60, 221, 345, 450, 982
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 2 cut(s) 474, 516
DpnI GATC 3 cut(s) 126, 211, 867
DpnII GATC 3 cut(s) 124, 209, 865
DraI TTTAAA 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 885
Eco47I GGWCC 3 cut(s) 115, 590, 845
Eco57I CTGAAG 4 cut(s) 149, 359, 561, 980
EcoO109I RGGNCCY 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 885
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 5 cut(s) 63, 224, 348, 453, 985
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 128
FatI CATG 5 cut(s) 59, 220, 344, 449, 981
FauI CCCGC 1 cut(s) 670
Fnu4HI GCNGC 2 cut(s) 525, 602
FokI GGATG 4 cut(s) 246, 262, 455, 854
Fsp4HI GCNGC 2 cut(s) 525, 602
FspBI CTAG 2 cut(s) 200, 788
GlaI GCGC 2 cut(s) 769, 771
GluI GCNGC 2 cut(s) 525, 602
HapII CCGG 3 cut(s) 263, 575, 605
HhaI GCGC 2 cut(s) 770, 772
Hin1II CATG 5 cut(s) 63, 224, 348, 453, 985
Hin6I GCGC 2 cut(s) 768, 770
HinP1I GCGC 2 cut(s) 768, 770
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 3 cut(s) 460, 631, 678
HpaII CCGG 3 cut(s) 263, 575, 605
Hpy188I TCNGA 7 cut(s) 129, 339, 423, 541, 781, 865, 960
Hpy188III TCNNGA 3 cut(s) 113, 788, 827
HpyAV CCTTC 7 cut(s) 200, 313, 536, 738, 870, 955, 961
HpyCH4III ACNGT 3 cut(s) 109, 364, 622
HpyCH4V TGCA 5 cut(s) 56, 348, 601, 820, 940
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 521, 662
HpyF3I CTNAG 2 cut(s) 474, 516
Hsp92II CATG 5 cut(s) 63, 224, 348, 453, 985
HspAI GCGC 2 cut(s) 768, 770
KflI GGGWCCC 1 cut(s) 115
KroI GCCGGC 1 cut(s) 604
KroNI GCCGGC 1 cut(s) 606
Kzo9I GATC 3 cut(s) 124, 209, 865
LmnI GCTCC 4 cut(s) 8, 38, 161, 861
Lsp1109I GCAGC 2 cut(s) 511, 613
LweI GCATC 2 cut(s) 65, 266
MaeI CTAG 2 cut(s) 200, 788
MaeIII GTNAC 2 cut(s) 31, 931
MalI GATC 3 cut(s) 126, 211, 867
MbiI CCGCTC 1 cut(s) 6
MboI GATC 3 cut(s) 124, 209, 865
MboII GAAGA 4 cut(s) 142, 378, 488, 490
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 663
MmeI TCCRAC 1 cut(s) 383
MnlI CCTC 9 cut(s) 80, 429, 511, 514, 712, 775, 832, 858, 986
MroNI GCCGGC 1 cut(s) 604
MroXI GAANNNNTTC 2 cut(s) 174, 971
MseI TTAA 3 cut(s) 186, 306, 612
MspI CCGG 3 cut(s) 263, 575, 605
MspR9I CCNGG 1 cut(s) 576
MvnI CGCG 1 cut(s) 770
MwoI GCNNNNNNNGC 3 cut(s) 70, 521, 662
NaeI GCCGGC 1 cut(s) 606
NciI CCSGG 1 cut(s) 576
NdeII GATC 3 cut(s) 124, 209, 865
NgoMIV GCCGGC 1 cut(s) 604
NlaIII CATG 5 cut(s) 63, 224, 348, 453, 985
NlaIV GGNNCC 2 cut(s) 116, 117
NspI RCATGY 1 cut(s) 985
NspV TTCGAA 1 cut(s) 458
PauI GCGCGC 1 cut(s) 768
PciI ACATGT 1 cut(s) 981
PdiI GCCGGC 1 cut(s) 606
PdmI GAANNNNTTC 2 cut(s) 174, 971
PfeI GAWTC 3 cut(s) 460, 631, 678
PkrI GCNGC 2 cut(s) 526, 603
PpuMI RGGWCCY 1 cut(s) 115
PscI ACATGT 1 cut(s) 981
PshBI ATTAAT 1 cut(s) 612
PsiI TTATAA 1 cut(s) 797
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 116, 117
PspPI GGNCC 3 cut(s) 115, 590, 845
PspPPI RGGWCCY 1 cut(s) 115
PstI CTGCAG 1 cut(s) 942
PsuI RGATCY 1 cut(s) 209
PteI GCGCGC 1 cut(s) 768
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
SaqAI TTAA 3 cut(s) 186, 306, 612
SatI GCNGC 2 cut(s) 525, 602
Sau3AI GATC 3 cut(s) 124, 209, 865
Sau96I GGNCC 3 cut(s) 115, 590, 845
ScrFI CCNGG 1 cut(s) 576
SduI GDGCHC 1 cut(s) 663
SfaNI GCATC 2 cut(s) 65, 266
SfcI CTRYAG 3 cut(s) 390, 738, 938
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 3 cut(s) 115, 590, 845
SsiI CCGC 3 cut(s) 6, 224, 663
SspMI CTAG 2 cut(s) 200, 788
StyD4I CCNGG 1 cut(s) 574
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 3 cut(s) 109, 364, 622
TaqI TCGA 2 cut(s) 458, 951
TfiI GAWTC 3 cut(s) 460, 631, 678
Tru1I TTAA 3 cut(s) 186, 306, 612
Tru9I TTAA 3 cut(s) 186, 306, 612
TscAI CASTG 2 cut(s) 112, 663
TseI GCWGC 2 cut(s) 524, 601
TspDTI ATGAA 5 cut(s) 219, 264, 422, 459, 670
TspGWI ACGGA 1 cut(s) 912
TspRI CASTG 2 cut(s) 112, 663
VpaK11BI GGWCC 3 cut(s) 115, 590, 845
VspI ATTAAT 1 cut(s) 612
XapI RAATTY 2 cut(s) 353, 533
XbaI TCTAGA 1 cut(s) 787
XceI RCATGY 1 cut(s) 985
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XmnI GAANNNNTTC 2 cut(s) 174, 971
XspI CTAG 2 cut(s) 200, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.