Rw0G002050

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00064
OG0000709
Physical Location & Seq
Reverse (-)
19192 .. 20877
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002050.1

Sequence Viewer

Length: 966 bp
ATGAGTGGAGAAAGGAAGGTTGTGTGTGTTACAGGAGCTTCTGGTTTCATAGCTTCATGGCTGGTGAAGCTCTTGTTACAGCGAGGTTATGTCGTCAAAGCCACCGTTCGTGACCCAAATAATCCGAAGAAAACAGAACACTTGCTCTCACTTGAGGGAGCAGAGGAAAGGCTTCATTTGTTCCAAGCAGACTTGTTAGAAGAGGGGTCTTTTGACCTTGTAGTTGATGGATGTGAAGGTGTTTTCCACACAGCATCTCCGGTACTATTTTCATCGATCAGCGACCCACAGGCAGAACTGATTGATCCTGCAGTAAAGGGAACGCTTAATGTTCTTAGATCGTGCGGGAAATTTCCAACAATCAAGAGGGTAGTTTTAACATCTTCCATGGCGGCAGTTATAGTCAATGGAAGACCATTAACCTCTAATGTGGTAGTTGATGAAACATGGTTTTCTGATCCACTTCTTTGTCAGGAGTTGAAGGAATGGTATTTTCTTTCGAAAACTTTAGCAGAGGAGGCTGCTTGGAAATTTGCTAAAGAAAACGGTATTGACATGGTGACCATAAATCCCTCGTATGTAATGGGTCCGCCCTTGCAGCCAACTCTTAATTTGAGTGTGGAGATGGTTCTGGATCTCAAGAAGGTAGCAATTGCCAATTATACATCTACTGATGTTAGAGATGTTGCCTCTGCACATATTCAAGCATTTGAAGTTCCTTCAGCCAGTGGAAGATATTGTTTAGTTGCAAATGTCACCCCTATTCACGAGGCTCTGAAGATTTTAAAGGAACTTCATCCTAGTTTGTGCCCACCTGAAATATGCGAGCATGATACCCCTTCTGCCCCAGAGTTTCAAATATCCCAGGAAAAAGCAAAAAGTTTGGGAGTTAGTTTCCTTCCGTTGGAAGTAAGTCTCAGGGACACTGTTGAATGCCTAAAGGAGAAGGGCTTCCTCAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

321

Amino Acids

35.2

Weight (kDa)

5.37

Isoelectric Point (pI)

29.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 105 5e-08 NmrA-like family
Epimerase PF01370 8 - 241 6.1e-21 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 236 4.2e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 200 2.8e-14 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 129 9.3e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 133 1.1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 345, 392, 590
AclWI GGATC 3 cut(s) 299, 452, 642
AcsI RAATTY 2 cut(s) 350, 530
AcuI CTGAAG 2 cut(s) 705, 797
AfaI GTAC 1 cut(s) 264
AfiI CCNNNNNNNGG 1 cut(s) 904
AgsI TTSAA 5 cut(s) 481, 704, 713, 857, 932
AjnI CCWGG 1 cut(s) 864
AluBI AGCT 3 cut(s) 38, 53, 70
AluI AGCT 3 cut(s) 38, 53, 70
Alw26I GTCTC 1 cut(s) 920
AlwI GGATC 3 cut(s) 299, 452, 642
ApeKI GCWGC 2 cut(s) 521, 598
ApoI RAATTY 2 cut(s) 350, 530
Asp700I GAANNNNTTC 2 cut(s) 171, 950
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 3 cut(s) 76, 571, 748
AsuII TTCGAA 1 cut(s) 500
AvaII GGWCC 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 812
BauI CACGAG 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 508, 610
BccI CCATC 2 cut(s) 221, 619
BciT130I CCWGG 1 cut(s) 866
BcoDI GTCTC 1 cut(s) 920
BfaI CTAG 1 cut(s) 801
BfmI CTRYAG 1 cut(s) 309
BisI GCNGC 3 cut(s) 393, 522, 599
BlsI GCNGC 3 cut(s) 394, 523, 600
Bme1390I CCNGG 1 cut(s) 866
Bme18I GGWCC 1 cut(s) 587
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 1 cut(s) 588
BmrFI CCNGG 1 cut(s) 866
BmsI GCATC 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 418
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 3 cut(s) 173, 623, 941
Bsa29I ATCGAT 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 387, 864
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 904
Bse1I ACTGG 1 cut(s) 726
BseBI CCWGG 1 cut(s) 866
BseCI ATCGAT 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 387, 864
BseGI GGATG 2 cut(s) 236, 796
BseLI CCNNNNNNNGG 1 cut(s) 904
BseMII CTCAG 1 cut(s) 931
BseNI ACTGG 1 cut(s) 726
BseRI GAGGAG 1 cut(s) 530
BseSI GKGCMC 1 cut(s) 812
BseXI GCAGC 2 cut(s) 508, 610
BsgI GTGCAG 1 cut(s) 678
BshVI ATCGAT 1 cut(s) 275
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 1 cut(s) 935
BslI CCNNNNNNNGG 1 cut(s) 904
BsmAI GTCTC 1 cut(s) 920
BsmFI GGGAC 1 cut(s) 935
BsmI GAATGC 1 cut(s) 938
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 1 cut(s) 812
Bsp143I GATC 5 cut(s) 276, 304, 338, 457, 634
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 3 cut(s) 345, 392, 590
BspCNI CTCAG 1 cut(s) 930
BspDI ATCGAT 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 588
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 3 cut(s) 299, 452, 642
BspT104I TTCGAA 1 cut(s) 500
BsrI ACTGG 1 cut(s) 726
BssECI CCNNGG 2 cut(s) 387, 864
BssMI GATC 5 cut(s) 276, 304, 338, 457, 634
BssSI CACGAG 1 cut(s) 767
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 767
Bst2UI CCWGG 1 cut(s) 866
Bst4CI ACNGT 3 cut(s) 106, 548, 928
Bst6I CTCTTC 1 cut(s) 195
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 827
BstDEI CTNAG 2 cut(s) 335, 917
BstDSI CCRYGG 1 cut(s) 387
BstEII GGTNACC 1 cut(s) 559
BstF5I GGATG 2 cut(s) 236, 796
BstKTI GATC 5 cut(s) 279, 307, 341, 460, 637
BstMAI GTCTC 1 cut(s) 920
BstMBI GATC 5 cut(s) 276, 304, 338, 457, 634
BstMWI GCNNNNNNNGC 3 cut(s) 67, 518, 598
BstNI CCWGG 1 cut(s) 866
BstPI GGTNACC 1 cut(s) 559
BstSCI CCNGG 1 cut(s) 864
BstSFI CTRYAG 1 cut(s) 309
BstSLI GKGCMC 1 cut(s) 812
BstV1I GCAGC 2 cut(s) 508, 610
BstV2I GAAGAC 1 cut(s) 418
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
Bsu15I ATCGAT 1 cut(s) 275
BsuTUI ATCGAT 1 cut(s) 275
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 2 cut(s) 236, 796
BtsIMutI CAGTG 2 cut(s) 733, 924
Cac8I GCNNGC 1 cut(s) 827
Cfr13I GGNCC 1 cut(s) 587
ClaI ATCGAT 1 cut(s) 275
Csp6I GTAC 1 cut(s) 263
CviAII CATG 5 cut(s) 57, 388, 447, 556, 830
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 2 cut(s) 335, 917
DpnI GATC 5 cut(s) 278, 306, 340, 459, 636
DpnII GATC 5 cut(s) 276, 304, 338, 457, 634
DraI TTTAAA 1 cut(s) 786
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 2 cut(s) 705, 797
Eco91I GGTNACC 1 cut(s) 559
EcoO65I GGTNACC 1 cut(s) 559
EcoRII CCWGG 1 cut(s) 864
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 60, 391, 450, 559, 833
FaqI GGGAC 1 cut(s) 935
FatI CATG 5 cut(s) 56, 387, 446, 555, 829
FauI CCCGC 1 cut(s) 338
Fnu4HI GCNGC 3 cut(s) 393, 522, 599
FokI GGATG 2 cut(s) 243, 783
Fsp4HI GCNGC 3 cut(s) 393, 522, 599
FspBI CTAG 1 cut(s) 801
GluI GCNGC 3 cut(s) 393, 522, 599
HapII CCGG 1 cut(s) 260
Hin1II CATG 5 cut(s) 60, 391, 450, 559, 833
HpaII CCGG 1 cut(s) 260
HphI GGTGA 3 cut(s) 76, 571, 748
Hpy188I TCNGA 3 cut(s) 126, 457, 777
Hpy188III TCNNGA 6 cut(s) 110, 364, 473, 632, 640, 767
HpyAV CCTTC 8 cut(s) 10, 230, 475, 637, 729, 849, 908, 940
HpyCH4III ACNGT 3 cut(s) 106, 548, 928
HpyCH4V TGCA 4 cut(s) 311, 598, 695, 749
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 518, 598
HpyF3I CTNAG 2 cut(s) 335, 917
Hsp92II CATG 5 cut(s) 60, 391, 450, 559, 833
Kzo9I GATC 5 cut(s) 276, 304, 338, 457, 634
LmnI GCTCC 2 cut(s) 35, 158
Lsp1109I GCAGC 2 cut(s) 508, 610
LweI GCATC 1 cut(s) 263
MaeI CTAG 1 cut(s) 801
MaeIII GTNAC 5 cut(s) 28, 75, 110, 559, 754
MalI GATC 5 cut(s) 278, 306, 340, 459, 636
MboI GATC 5 cut(s) 276, 304, 338, 457, 634
MboII GAAGA 6 cut(s) 139, 212, 375, 423, 744, 790
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 1 cut(s) 812
MluCI AATT 5 cut(s) 350, 530, 610, 651, 658
MmeI TCCRAC 2 cut(s) 380, 885
MroXI GAANNNNTTC 2 cut(s) 171, 950
MseI TTAA 5 cut(s) 327, 377, 419, 609, 785
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 866
MunI CAATTG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 938
MvaI CCWGG 1 cut(s) 866
MwoI GCNNNNNNNGC 3 cut(s) 67, 518, 598
NcoI CCATGG 1 cut(s) 387
NdeII GATC 5 cut(s) 276, 304, 338, 457, 634
NlaIII CATG 5 cut(s) 60, 391, 450, 559, 833
NlaIV GGNNCC 1 cut(s) 588
NmuCI GTSAC 3 cut(s) 110, 559, 754
NspV TTCGAA 1 cut(s) 500
PctI GAATGC 1 cut(s) 938
PdmI GAANNNNTTC 2 cut(s) 171, 950
PkrI GCNGC 3 cut(s) 394, 523, 600
Psp6I CCWGG 1 cut(s) 864
PspEI GGTNACC 1 cut(s) 559
PspGI CCWGG 1 cut(s) 864
PspN4I GGNNCC 1 cut(s) 588
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 1 cut(s) 313
PsuI RGATCY 1 cut(s) 634
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
SaqAI TTAA 5 cut(s) 327, 377, 419, 609, 785
SatI GCNGC 3 cut(s) 393, 522, 599
Sau3AI GATC 5 cut(s) 276, 304, 338, 457, 634
Sau96I GGNCC 1 cut(s) 587
ScrFI CCNGG 1 cut(s) 866
SduI GDGCHC 1 cut(s) 812
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 500
SinI GGWCC 1 cut(s) 587
SmlI CTYRAG 3 cut(s) 152, 638, 956
SmoI CTYRAG 3 cut(s) 152, 638, 956
Sse9I AATT 5 cut(s) 350, 530, 610, 651, 658
SsiI CCGC 3 cut(s) 345, 392, 590
SspMI CTAG 1 cut(s) 801
StyD4I CCNGG 1 cut(s) 864
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 3 cut(s) 106, 548, 928
TaqI TCGA 2 cut(s) 275, 500
TasI AATT 5 cut(s) 350, 530, 610, 651, 658
TauI GCSGC 1 cut(s) 395
Tru1I TTAA 5 cut(s) 327, 377, 419, 609, 785
Tru9I TTAA 5 cut(s) 327, 377, 419, 609, 785
TscAI CASTG 2 cut(s) 733, 931
TseFI GTSAC 3 cut(s) 110, 559, 754
TseI GCWGC 2 cut(s) 521, 598
Tsp45I GTSAC 3 cut(s) 110, 559, 754
TspDTI ATGAA 6 cut(s) 37, 45, 164, 261, 456, 785
TspGWI ACGGA 1 cut(s) 891
TspRI CASTG 2 cut(s) 733, 931
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 2 cut(s) 350, 530
XmnI GAANNNNTTC 2 cut(s) 171, 950
XspI CTAG 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.