MD04G1006100.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000709
Physical Location & Seq
Reverse (-)
705200 .. 708081
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1006100.v1.1.491

Sequence Viewer

Length: 948 bp
ATGAGTGGAGAAACGAAGGTTGTGTGTGTAACAGGAGCCTCCGGCTACGTAGCGTCATGGCTTGTGAAGCTCTTACTACAAAGAGGTTATGCTGTCAAAGCTTCTGTTCGTGATCCGAATGATACAAACAAAACAGAACACTTACTCTCACTAGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAAGCAGATTTACTAGAAGAAGGATCTTTCGACGCTGTAGTTGATGAATGTGTTTGTGTTTTTCATACAGCATCTCCTGTTCAATTTTCGGCTGAATTAGTTGAGCCTGCAGTTAAAGGAACGCTTAATGTTCTACAATCATGCGTGAAATTCCCCGCTGTTAAGAGAGTGGTTATGACATCTTCTATGGCTGCAGTAATGTCGACTGGAAAACCTCTGACCTCTGATGTGGTTGTGGACGAAACATACTATTCGGATCCACTTTTTTGTGAGAAGATCAAGCAATGGTATCGTCTCTCAAAAACTTTAGCTGAGCAGGCCGCCTGGAAATTTGCCGAAGAAAATGGGATTGACTTGGTTACATTACATCCAGGGTTTACGATTGGTCCACTATTACGGCCAACTCTTAATATCTCCATCAAGTTTTTTCAGAATCTCATGAGTGGTATCGAAACACCGTTTATAAACTACCCATTTGTAGACGTTAGAGATATTGCCTCTGCTCATATTCAAGCATTTGAAGTTCCTTCAGCTAAAGGTAGATATTGTTTAGTTGCCCAGGTTGCTGACGGACCCGATACTCTCAAGATTTTGCAACAACTTTACCCCACATTGTGCCTTCCGGAGTTTGGCAATCCTTCTGACTCAAAGTTTCAAGTGTCCATAGAGAAAGCAAAAGGTTTGGGAATCAATTTCCTTCCTCTGGAAAAGAGTATCAGAGACACTGTTGAAAGCCTCAAGGAGAAGGGCTTCCTCAACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

316

Amino Acids

34.69

Weight (kDa)

5.82

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 93 1.2e-07 NmrA-like family
Epimerase PF01370 8 - 239 5.6e-21 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 233 2.8e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 190 3.9e-12 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 123 2.9e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 133 3.9e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 647
AccI GTMKAC 2 cut(s) 386, 663
AccIII TCCGGA 1 cut(s) 805
AciI CCGC 2 cut(s) 339, 504
AclWI GGATC 4 cut(s) 107, 214, 434, 447
AcoI YGGCCR 1 cut(s) 581
AcsI RAATTY 2 cut(s) 332, 512
AcuI CTGAAG 1 cut(s) 696
AdeI CACNNNGTG 1 cut(s) 798
AfiI CCNNNNNNNGG 2 cut(s) 812, 886
AgsI TTSAA 6 cut(s) 184, 266, 695, 704, 839, 914
AjnI CCWGG 3 cut(s) 506, 553, 741
AluBI AGCT 4 cut(s) 70, 101, 494, 716
AluI AGCT 4 cut(s) 70, 101, 494, 716
Alw26I GTCTC 2 cut(s) 482, 897
AlwI GGATC 4 cut(s) 107, 214, 434, 447
Aor13HI TCCGGA 1 cut(s) 805
AoxI GGCC 2 cut(s) 501, 581
ApeKI GCWGC 1 cut(s) 374
ApoI RAATTY 2 cut(s) 332, 512
Asp700I GAANNNNTTC 2 cut(s) 171, 932
AspS9I GGNCC 2 cut(s) 569, 755
AvaII GGWCC 2 cut(s) 569, 755
BamHI GGATCC 1 cut(s) 439
BbvI GCAGC 1 cut(s) 361
BccI CCATC 2 cut(s) 149, 608
BceAI ACGGC 1 cut(s) 596
BcgI CGANNNNNNTGC 4 cut(s) 366, 400, 455, 489
BciT130I CCWGG 3 cut(s) 508, 555, 743
BcoDI GTCTC 2 cut(s) 482, 897
BfaI CTAG 2 cut(s) 152, 197
BfmI CTRYAG 3 cut(s) 219, 291, 375
BisI GCNGC 2 cut(s) 375, 504
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 2 cut(s) 376, 505
Bme1390I CCNGG 3 cut(s) 508, 555, 743
Bme18I GGWCC 2 cut(s) 569, 755
BmgT120I GGNCC 2 cut(s) 569, 755
BmiI GGNNCC 3 cut(s) 37, 441, 757
BmrFI CCNGG 3 cut(s) 508, 555, 743
BmsI GCATC 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 752, 905
BsaAI YACGTR 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 554, 741
BsaWI WCCGGW 1 cut(s) 805
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 2 cut(s) 812, 886
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 473
BseAI TCCGGA 1 cut(s) 805
BseBI CCWGG 3 cut(s) 508, 555, 743
BseDI CCNNGG 2 cut(s) 554, 741
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 2 cut(s) 812, 886
BseMI GCAATG 1 cut(s) 473
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 1 cut(s) 394
BseXI GCAGC 1 cut(s) 361
BshFI GGCC 2 cut(s) 503, 583
BsiSI CCGG 2 cut(s) 42, 806
BslI CCNNNNNNNGG 2 cut(s) 812, 886
BsmAI GTCTC 2 cut(s) 482, 897
BsmBI CGTCTC 1 cut(s) 482
BsnI GGCC 2 cut(s) 503, 583
Bsp13I TCCGGA 1 cut(s) 805
Bsp143I GATC 4 cut(s) 112, 206, 439, 459
Bsp1720I GCTNAGC 1 cut(s) 495
BspACI CCGC 2 cut(s) 339, 504
BspANI GGCC 2 cut(s) 503, 583
BspCNI CTCAG 1 cut(s) 487
BspEI TCCGGA 1 cut(s) 805
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 3 cut(s) 37, 441, 757
BspMAI CTGCAG 2 cut(s) 295, 379
BspPI GGATC 4 cut(s) 107, 214, 434, 447
BsrDI GCAATG 1 cut(s) 473
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 2 cut(s) 554, 741
BssMI GATC 4 cut(s) 112, 206, 439, 459
Bst2UI CCWGG 3 cut(s) 508, 555, 743
Bst4CI ACNGT 2 cut(s) 642, 910
BstBAI YACGTR 1 cut(s) 49
BstC8I GCNNGC 2 cut(s) 291, 501
BstDEI CTNAG 1 cut(s) 495
BstF5I GGATG 1 cut(s) 550
BstKTI GATC 4 cut(s) 115, 209, 442, 462
BstMAI GTCTC 2 cut(s) 482, 897
BstMBI GATC 4 cut(s) 112, 206, 439, 459
BstMWI GCNNNNNNNGC 4 cut(s) 67, 98, 500, 746
BstNI CCWGG 3 cut(s) 508, 555, 743
BstSCI CCNGG 3 cut(s) 506, 553, 741
BstSFI CTRYAG 3 cut(s) 219, 291, 375
BstSNI TACGTA 1 cut(s) 49
BstV1I GCAGC 1 cut(s) 361
BstX2I RGATCY 2 cut(s) 206, 439
BstYI RGATCY 2 cut(s) 206, 439
BsuRI GGCC 2 cut(s) 503, 583
BtsCI GGATG 1 cut(s) 550
BtsIMutI CAGTG 1 cut(s) 906
Cac8I GCNNGC 2 cut(s) 291, 501
CciI TCATGA 1 cut(s) 621
Cfr13I GGNCC 2 cut(s) 569, 755
CseI GACGC 2 cut(s) 42, 224
CviAII CATG 3 cut(s) 57, 324, 622
DdeI CTNAG 1 cut(s) 495
DpnI GATC 4 cut(s) 114, 208, 441, 461
DpnII GATC 4 cut(s) 112, 206, 439, 459
DraIII CACNNNGTG 1 cut(s) 798
EaeI YGGCCR 1 cut(s) 581
Eco105I TACGTA 1 cut(s) 49
Eco47I GGWCC 2 cut(s) 569, 755
Eco57I CTGAAG 1 cut(s) 696
EcoRII CCWGG 3 cut(s) 506, 553, 741
Esp3I CGTCTC 1 cut(s) 482
FaeI CATG 3 cut(s) 60, 327, 625
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 291, 323
FatI CATG 3 cut(s) 56, 323, 621
FauI CCCGC 1 cut(s) 346
FblI GTMKAC 2 cut(s) 386, 663
Fnu4HI GCNGC 2 cut(s) 375, 504
FokI GGATG 1 cut(s) 537
Fsp4HI GCNGC 2 cut(s) 375, 504
FspBI CTAG 2 cut(s) 152, 197
GluI GCNGC 2 cut(s) 375, 504
HaeIII GGCC 2 cut(s) 503, 583
HapII CCGG 2 cut(s) 42, 806
HgaI GACGC 2 cut(s) 42, 224
Hin1II CATG 3 cut(s) 60, 327, 625
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 3 cut(s) 616, 827, 870
HpaII CCGG 2 cut(s) 42, 806
Hpy166II GTNNAC 5 cut(s) 387, 421, 561, 572, 664
Hpy188I TCNGA 7 cut(s) 117, 402, 409, 439, 615, 826, 902
Hpy188III TCNNGA 5 cut(s) 110, 622, 769, 806, 887
Hpy8I GTNNAC 5 cut(s) 387, 421, 561, 572, 664
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 7 cut(s) 10, 197, 720, 812, 831, 890, 922
HpyCH4III ACNGT 2 cut(s) 642, 910
HpyCH4IV ACGT 2 cut(s) 48, 666
HpyCH4V TGCA 3 cut(s) 293, 377, 778
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 98, 500, 746
HpyF3I CTNAG 1 cut(s) 495
HpySE526I ACGT 2 cut(s) 48, 666
Hsp92II CATG 3 cut(s) 60, 327, 625
Kpn2I TCCGGA 1 cut(s) 805
Kzo9I GATC 4 cut(s) 112, 206, 439, 459
LmnI GCTCC 2 cut(s) 35, 158
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 1 cut(s) 263
MaeI CTAG 2 cut(s) 152, 197
MaeII ACGT 2 cut(s) 48, 666
MaeIII GTNAC 2 cut(s) 28, 541
MalI GATC 4 cut(s) 114, 208, 441, 461
MboI GATC 4 cut(s) 112, 206, 439, 459
MboII GAAGA 4 cut(s) 212, 357, 469, 533
MflI RGATCY 2 cut(s) 206, 439
MluCI AATT 5 cut(s) 266, 278, 332, 512, 874
MlyI GAGTC 1 cut(s) 821
MnlI CCTC 8 cut(s) 49, 77, 408, 415, 691, 894, 929, 947
MroI TCCGGA 1 cut(s) 805
MroXI GAANNNNTTC 2 cut(s) 171, 932
MseI TTAA 4 cut(s) 297, 309, 345, 591
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 2 cut(s) 42, 806
MspR9I CCNGG 3 cut(s) 508, 555, 743
MvaI CCWGG 3 cut(s) 508, 555, 743
MwoI GCNNNNNNNGC 4 cut(s) 67, 98, 500, 746
NdeII GATC 4 cut(s) 112, 206, 439, 459
NlaIII CATG 3 cut(s) 60, 327, 625
NlaIV GGNNCC 3 cut(s) 37, 441, 757
PagI TCATGA 1 cut(s) 621
PdmI GAANNNNTTC 2 cut(s) 171, 932
PfeI GAWTC 2 cut(s) 616, 870
PkrI GCNGC 2 cut(s) 376, 505
PleI GAGTC 1 cut(s) 821
PpsI GAGTC 1 cut(s) 821
Ppu21I YACGTR 1 cut(s) 49
PsiI TTATAA 1 cut(s) 647
Psp6I CCWGG 3 cut(s) 506, 553, 741
PspGI CCWGG 3 cut(s) 506, 553, 741
PspN4I GGNNCC 3 cut(s) 37, 441, 757
PspPI GGNCC 2 cut(s) 569, 755
PstI CTGCAG 2 cut(s) 295, 379
PsuI RGATCY 2 cut(s) 206, 439
SalI GTCGAC 1 cut(s) 385
SaqAI TTAA 4 cut(s) 297, 309, 345, 591
SatI GCNGC 2 cut(s) 375, 504
Sau3AI GATC 4 cut(s) 112, 206, 439, 459
Sau96I GGNCC 2 cut(s) 569, 755
SchI GAGTC 1 cut(s) 821
ScrFI CCNGG 3 cut(s) 508, 555, 743
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 3 cut(s) 219, 291, 375
SinI GGWCC 2 cut(s) 569, 755
SmlI CTYRAG 2 cut(s) 767, 920
SmoI CTYRAG 2 cut(s) 767, 920
SnaBI TACGTA 1 cut(s) 49
Sse9I AATT 5 cut(s) 266, 278, 332, 512, 874
SsiI CCGC 2 cut(s) 339, 504
SspMI CTAG 2 cut(s) 152, 197
StyD4I CCNGG 3 cut(s) 506, 553, 741
TaaI ACNGT 2 cut(s) 642, 910
TaiI ACGT 2 cut(s) 51, 669
TaqI TCGA 3 cut(s) 213, 386, 633
TasI AATT 5 cut(s) 266, 278, 332, 512, 874
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 2 cut(s) 616, 870
Tru1I TTAA 4 cut(s) 297, 309, 345, 591
Tru9I TTAA 4 cut(s) 297, 309, 345, 591
TscAI CASTG 1 cut(s) 913
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 3 cut(s) 164, 236, 243
TspGWI ACGGA 1 cut(s) 768
TspRI CASTG 1 cut(s) 913
VpaK11BI GGWCC 2 cut(s) 569, 755
XapI RAATTY 2 cut(s) 332, 512
XmiI GTMKAC 2 cut(s) 386, 663
XmnI GAANNNNTTC 2 cut(s) 171, 932
XspI CTAG 2 cut(s) 152, 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.