MD07G1020300.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000709
Physical Location & Seq
Reverse (-)
1726059 .. 1729658
3600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1020300.v1.1.491

Sequence Viewer

Length: 966 bp
ATGAGGAAGGTGGTGTGCGTAACAGGAGCCTCCGGTTACATAGCATCATGGCTGGTGAAGCTCTTACTGCAACGAGGTTATATTGTCAAAGCCACTGTTCGTGAACCAAATGATTCAAGGAAAACAGAACACCTGCTCGCACTTGATGGAGCAAAAGAAAGGCTTCAGTTGTTCAAAGCAAATTTATTAGAAGAAGGATCTTTCGACCCTGCAGTCGATGGATGTGAAGGTGTTTTTCATACAGCATCGCCTGTACAACTTTCAGCCGCTGATCCGCAGACAGAACTAACTGACCCTGCAGTGAAGGGCACACTCAATGTCCTAAAATCGTGCGTAAAATTTCCTACTGTCAAGAGAGTGATTTTAACATCTTCCATGGCGGCAGTCATGGTGAATGGAAGACCTTTGAACCCTGATGTGGTAATTGACGAAACATGGTATTCGGATCAAGCAATTTGTGAGCAGTTGAAGGAATGGTATTTTCTTTCAAAAACTTTAGCTGAGGAGGCTGCTTGGAAATTTTCCAAAGAAAATGGGATTGACTTGGTTACAATCAATCCATCATATGTGATTGGTCCGCTCCTACAGCCAACTCTTAATCTCTCTGTGGAGATGATTCTGAATCTCAAAAATGATATCCCAAGTATAATCAGTTCAAATTATCCATCTAGTGATGTCAGAGATGTTGCCTTTGCTCATGTTCAAGCATTTGAAGTCCCTTCGGCTAGTGGCAGATATTGTTTCGTTGGCCATGTTACACCCATGTCCAAGGCTCTGAGTATTTTACATGAACTCCATCCCACTTTTTTCCCACCTGAAAAATGTGATGATGACAAACCTTGTGAACCAGGCTATCAAATATCAAAGGAAAAAGCAAAAAGTTTGGGAGTTAATTTTCTTCCATTGGAAGTAAGTCTTAGGGACACTATTGAAAGCCTCAAAGAGAAGGGCTTCCTCAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

322

Amino Acids

35.45

Weight (kDa)

5.77

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 2 - 88 1.9e-08 NmrA-like family
Epimerase PF01370 5 - 242 1.1e-19 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 7 - 237 8.1e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 7 - 192 1.4e-11 Male sterility protein
GDP_Man_Dehyd PF16363 7 - 125 1.5e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 9 - 130 2.3e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 141
AasI GACNNNNNNGTC 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 141
AccBSI CCGCTC 1 cut(s) 580
AciI CCGC 4 cut(s) 267, 275, 380, 578
AclWI GGATC 3 cut(s) 205, 266, 453
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 3 cut(s) 181, 338, 518
AcuI CTGAAG 1 cut(s) 149
AfaI GTAC 1 cut(s) 255
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 9 cut(s) 117, 175, 409, 469, 489, 657, 704, 713, 932
AjnI CCWGG 1 cut(s) 847
AluBI AGCT 3 cut(s) 61, 500, 961
AluI AGCT 3 cut(s) 61, 500, 961
AlwI GGATC 3 cut(s) 205, 266, 453
AlwNI CAGNNNCTG 1 cut(s) 269
AoxI GGCC 1 cut(s) 748
ApeKI GCWGC 1 cut(s) 509
ApoI RAATTY 3 cut(s) 181, 338, 518
Asp700I GAANNNNTTC 2 cut(s) 162, 950
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 2 cut(s) 67, 403
AvaII GGWCC 1 cut(s) 575
BaeGI GKGCMC 1 cut(s) 311
BalI TGGCCA 1 cut(s) 750
BbsI GAAGAC 1 cut(s) 406
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 1 cut(s) 496
BccI CCATC 5 cut(s) 140, 212, 568, 673, 804
BciT130I CCWGG 1 cut(s) 849
BfaI CTAG 2 cut(s) 669, 726
BfmI CTRYAG 3 cut(s) 210, 297, 584
BfuAI ACCTGC 1 cut(s) 141
BisI GCNGC 3 cut(s) 267, 381, 510
BlsI GCNGC 3 cut(s) 268, 382, 511
Bme1390I CCNGG 1 cut(s) 849
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 849
BmsI GCATC 2 cut(s) 53, 254
BpiI GAAGAC 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 941
BsaJI CCNNGG 2 cut(s) 375, 768
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 1 cut(s) 418
BseBI CCWGG 1 cut(s) 849
BseDI CCNNGG 2 cut(s) 375, 768
BseGI GGATG 2 cut(s) 227, 796
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 492, 767
BseRI GAGGAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 311
BseXI GCAGC 1 cut(s) 496
BshFI GGCC 1 cut(s) 750
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 2 cut(s) 701, 935
BslI CCNNNNNNNGG 1 cut(s) 418
BsmFI GGGAC 2 cut(s) 701, 935
BsnI GGCC 1 cut(s) 750
Bsp1286I GDGCHC 1 cut(s) 311
Bsp1407I TGTACA 1 cut(s) 253
Bsp143I GATC 3 cut(s) 197, 271, 445
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 4 cut(s) 267, 275, 380, 578
BspANI GGCC 1 cut(s) 750
BspCNI CTCAG 2 cut(s) 493, 768
BspLI GGNNCC 1 cut(s) 28
BspMAI CTGCAG 2 cut(s) 214, 301
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 3 cut(s) 205, 266, 453
BsrBI CCGCTC 1 cut(s) 580
BsrGI TGTACA 1 cut(s) 253
BssECI CCNNGG 2 cut(s) 375, 768
BssMI GATC 3 cut(s) 197, 271, 445
BssT1I CCWWGG 2 cut(s) 375, 768
Bst2UI CCWGG 1 cut(s) 849
Bst4CI ACNGT 2 cut(s) 97, 349
BstAUI TGTACA 1 cut(s) 253
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 3 cut(s) 501, 776, 917
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 227, 796
BstKTI GATC 3 cut(s) 200, 274, 448
BstMBI GATC 3 cut(s) 197, 271, 445
BstMWI GCNNNNNNNGC 4 cut(s) 58, 67, 506, 586
BstNI CCWGG 1 cut(s) 849
BstSCI CCNGG 1 cut(s) 847
BstSFI CTRYAG 3 cut(s) 210, 297, 584
BstSLI GKGCMC 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 496
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BsuRI GGCC 1 cut(s) 750
BtgI CCRYGG 1 cut(s) 375
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 2 cut(s) 227, 796
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 2 cut(s) 93, 306
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 1 cut(s) 138
CaiI CAGNNNCTG 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 575
Csp6I GTAC 1 cut(s) 254
CviAII CATG 8 cut(s) 48, 376, 388, 435, 698, 752, 763, 788
CviQI GTAC 1 cut(s) 254
DdeI CTNAG 3 cut(s) 501, 776, 917
DpnI GATC 3 cut(s) 199, 273, 447
DpnII GATC 3 cut(s) 197, 271, 445
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 1 cut(s) 748
Eco130I CCWWGG 2 cut(s) 375, 768
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 847
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 2 cut(s) 375, 768
ErhI CCWWGG 2 cut(s) 375, 768
FaeI CATG 8 cut(s) 51, 379, 391, 438, 701, 755, 766, 791
FalI AAGNNNNNCTT 4 cut(s) 147, 179, 900, 932
FaqI GGGAC 2 cut(s) 701, 935
FatI CATG 8 cut(s) 47, 375, 387, 434, 697, 751, 762, 787
FauNDI CATATG 1 cut(s) 565
Fnu4HI GCNGC 3 cut(s) 267, 381, 510
FokI GGATG 2 cut(s) 234, 783
Fsp4HI GCNGC 3 cut(s) 267, 381, 510
FspBI CTAG 2 cut(s) 669, 726
GluI GCNGC 3 cut(s) 267, 381, 510
HaeIII GGCC 1 cut(s) 750
HapII CCGG 1 cut(s) 33
Hin1II CATG 8 cut(s) 51, 379, 391, 438, 701, 755, 766, 791
HindIII AAGCTT 1 cut(s) 959
HinfI GANTC 3 cut(s) 113, 616, 622
HpaII CCGG 1 cut(s) 33
HphI GGTGA 2 cut(s) 67, 403
Hpy166II GTNNAC 2 cut(s) 104, 845
Hpy188I TCNGA 4 cut(s) 445, 621, 680, 777
Hpy188III TCNNGA 2 cut(s) 101, 352
Hpy8I GTNNAC 2 cut(s) 104, 845
HpyAV CCTTC 6 cut(s) 188, 221, 298, 463, 729, 940
HpyCH4III ACNGT 2 cut(s) 97, 349
HpyCH4V TGCA 3 cut(s) 70, 212, 299
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 67, 506, 586
HpyF3I CTNAG 3 cut(s) 501, 776, 917
Hsp92II CATG 8 cut(s) 51, 379, 391, 438, 701, 755, 766, 791
Kzo9I GATC 3 cut(s) 197, 271, 445
LmnI GCTCC 3 cut(s) 26, 149, 585
Lsp1109I GCAGC 1 cut(s) 496
LweI GCATC 2 cut(s) 53, 254
MaeI CTAG 2 cut(s) 669, 726
MaeIII GTNAC 4 cut(s) 19, 35, 547, 754
MalI GATC 3 cut(s) 199, 273, 447
MbiI CCGCTC 1 cut(s) 580
MboI GATC 3 cut(s) 197, 271, 445
MboII GAAGA 4 cut(s) 203, 363, 411, 890
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 1 cut(s) 311
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 7 cut(s) 181, 338, 423, 453, 518, 658, 892
MluNI TGGCCA 1 cut(s) 750
MnlI CCTC 6 cut(s) 40, 68, 496, 499, 947, 965
Mox20I TGGCCA 1 cut(s) 750
MroXI GAANNNNTTC 2 cut(s) 162, 950
MscI TGGCCA 1 cut(s) 750
MseI TTAA 3 cut(s) 365, 597, 891
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 849
MvaI CCWGG 1 cut(s) 849
MwoI GCNNNNNNNGC 4 cut(s) 58, 67, 506, 586
NcoI CCATGG 1 cut(s) 375
NdeI CATATG 1 cut(s) 565
NdeII GATC 3 cut(s) 197, 271, 445
NlaIII CATG 8 cut(s) 51, 379, 391, 438, 701, 755, 766, 791
NlaIV GGNNCC 1 cut(s) 28
PaqCI CACCTGC 1 cut(s) 141
PdmI GAANNNNTTC 2 cut(s) 162, 950
PfeI GAWTC 3 cut(s) 113, 616, 622
PkrI GCNGC 3 cut(s) 268, 382, 511
Psp6I CCWGG 1 cut(s) 847
PspGI CCWGG 1 cut(s) 847
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 1 cut(s) 575
PsrI GAACNNNNNNTAC 2 cut(s) 637, 669
PstI CTGCAG 2 cut(s) 214, 301
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 197
RsaI GTAC 1 cut(s) 255
RsaNI GTAC 1 cut(s) 254
SaqAI TTAA 3 cut(s) 365, 597, 891
SatI GCNGC 3 cut(s) 267, 381, 510
Sau3AI GATC 3 cut(s) 197, 271, 445
Sau96I GGNCC 1 cut(s) 575
ScrFI CCNGG 1 cut(s) 849
SduI GDGCHC 1 cut(s) 311
SfaNI GCATC 2 cut(s) 53, 254
SfcI CTRYAG 3 cut(s) 210, 297, 584
SinI GGWCC 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
Sse9I AATT 7 cut(s) 181, 338, 423, 453, 518, 658, 892
SsiI CCGC 4 cut(s) 267, 275, 380, 578
SspMI CTAG 2 cut(s) 669, 726
StyD4I CCNGG 1 cut(s) 847
StyI CCWWGG 2 cut(s) 375, 768
TaaI ACNGT 2 cut(s) 97, 349
TaqI TCGA 2 cut(s) 204, 216
TasI AATT 7 cut(s) 181, 338, 423, 453, 518, 658, 892
TatI WGTACW 1 cut(s) 253
TauI GCSGC 2 cut(s) 269, 383
TfiI GAWTC 3 cut(s) 113, 616, 622
Tru1I TTAA 3 cut(s) 365, 597, 891
Tru9I TTAA 3 cut(s) 365, 597, 891
TscAI CASTG 2 cut(s) 100, 306
TseI GCWGC 1 cut(s) 509
TspDTI ATGAA 2 cut(s) 227, 804
TspRI CASTG 2 cut(s) 100, 306
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 3 cut(s) 181, 338, 518
XmnI GAANNNNTTC 2 cut(s) 162, 950
XspI CTAG 2 cut(s) 669, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.