pycom02g25590

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000709
Physical Location & Seq
Forward (+)
23444541 .. 23448279
3739 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25590.1

Sequence Viewer

Length: 1008 bp
ATGGTTAAAAAAAAGGCCCCTCATGAATGTGGGGCCCTAGGTGCTGACTTTATTGACCGTTCTGATCTCTCACCTCACCTCACCGTGAAAACAACTCTGGCAAAACTAAATATAAAGCTTTTTTTTCTGAGCTTTCACAGAGAGAGAGAGAGAGAGAGAGGGAGAGAGATCAAGAGAGAGGGGATGAGGAAGGTGGTGTGTGTAACAGGAGGCTCCGGTTACATAGCAGCATGGCTGGTGAAGCTCTTACTGGAACGAGGTTATATTGTCAAAGCCACTGTTCGTGAACCAAATGATTCAAAGAAAACAGAACACCTGCTCTCACTTGATGGAGCAAAAGAAAGGCTTCAATTGTTCAAAGCAAATTTATTAGAAGAACGATCCTTCGACTCTGCAGTCGATGGATGTGAAGGTGTTTTTCATACAGCATCACCTGTACAATTTTCAGCCGCTGATCCGCAGGCAGAACTAATTGGCCCTGCAGTGAAGGGAACACTCAATGTTCTAAAATCGTGCGTAAAATTTCCTACTGTCAAGAGAGTGGTTTTAACATCTTCTATGGCTGCAGTCATAGTGAATGGAAGACCTTTGAACCCTGATGTGGTAGTTGACGAAACATGGTATTCGGATCAAGCTGTTTGTGCGCAGTTGAAGGAATGGTATTTTCTTTCAAAAACTATAGCTGAGGAGGCGGCTTGGAAATTTTCCAAAGAAAATGGGATTGACTTGGTTACAATAAACCCATCGTATGTGATTGGTCCGCTCCTACAGCCAACTCTTAATCTCACTGTGAAGATGATTCTGAATCTCAAAAATGATGTCCACGATGTAATCAGTTCAAATTATATATCTAGTGATGTTAGAGATGTTGCCTATGCTCATGTTCAAGCATTTGAAGTCCCTTCGGCTAGTGGCAGATATTGTTTCGTTGGTCATGTTACACCCGTGTCCAAGGTTCTGAATATTTTGCATAAACTCCATCCCACTTTTTTCCCACCTGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

336

Amino Acids

37.24

Weight (kDa)

8.88

Isoelectric Point (pI)

29.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 63 - 147 1.5e-08 NmrA-like family
Epimerase PF01370 66 - 274 9.2e-19 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 68 - 299 1.1e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 68 - 257 2.1e-12 Male sterility protein
GDP_Man_Dehyd PF16363 68 - 187 8.3e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 70 - 190 1.4e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 324
AasI GACNNNNNNGTC 1 cut(s) 395
Acc16I TGCGCA 1 cut(s) 645
Acc36I ACCTGC 1 cut(s) 324
AccBSI CCGCTC 1 cut(s) 763
AciI CCGC 4 cut(s) 450, 458, 692, 761
AclWI GGATC 3 cut(s) 375, 449, 636
AcsI RAATTY 3 cut(s) 364, 521, 701
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 1 cut(s) 601
AgsI TTSAA 9 cut(s) 300, 350, 358, 592, 652, 672, 840, 887, 896
AluBI AGCT 5 cut(s) 118, 132, 244, 635, 683
AluI AGCT 5 cut(s) 118, 132, 244, 635, 683
AlwI GGATC 3 cut(s) 375, 449, 636
AlwNI CAGNNNCTG 1 cut(s) 452
AoxI GGCC 3 cut(s) 15, 33, 475
ApaI GGGCCC 1 cut(s) 37
ApeKI GCWGC 2 cut(s) 227, 563
ApoI RAATTY 3 cut(s) 364, 521, 701
ArsI GACNNNNNNTTYG 2 cut(s) 804, 836
Asp700I GAANNNNTTC 1 cut(s) 345
AspA2I CCTAGG 1 cut(s) 37
AspLEI GCGC 1 cut(s) 646
AspS9I GGNCC 5 cut(s) 16, 33, 34, 476, 758
AsuHPI GGTGA 5 cut(s) 63, 68, 73, 250, 423
AvaII GGWCC 1 cut(s) 758
AvrII CCTAGG 1 cut(s) 37
BaeGI GKGCMC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 589
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 2 cut(s) 239, 550
BccI CCATC 4 cut(s) 323, 395, 751, 987
BfaI CTAG 3 cut(s) 38, 852, 909
BfmI CTRYAG 5 cut(s) 393, 480, 564, 678, 767
BfuAI ACCTGC 1 cut(s) 324
BisI GCNGC 4 cut(s) 228, 450, 564, 693
BlnI CCTAGG 1 cut(s) 37
BlsI GCNGC 4 cut(s) 229, 451, 565, 694
Bme18I GGWCC 1 cut(s) 758
BmgT120I GGNCC 5 cut(s) 16, 33, 34, 476, 758
BmiI GGNNCC 4 cut(s) 18, 34, 35, 214
BmsI GCATC 1 cut(s) 437
BpiI GAAGAC 1 cut(s) 589
Bpu10I CCTNAGC 1 cut(s) 684
BsaJI CCNNGG 2 cut(s) 37, 951
BsaWI WCCGGW 1 cut(s) 215
Bsc4I CCNNNNNNNGG 1 cut(s) 601
Bse1I ACTGG 1 cut(s) 255
BseDI CCNNGG 2 cut(s) 37, 951
BseGI GGATG 3 cut(s) 189, 410, 979
BseLI CCNNNNNNNGG 1 cut(s) 601
BseMII CTCAG 2 cut(s) 119, 675
BseNI ACTGG 1 cut(s) 255
BseRI GAGGAG 1 cut(s) 701
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 2 cut(s) 239, 550
BshFI GGCC 3 cut(s) 17, 35, 477
BsiSI CCGG 1 cut(s) 216
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 1 cut(s) 601
BsmFI GGGAC 1 cut(s) 884
BsnI GGCC 3 cut(s) 17, 35, 477
Bsp120I GGGCCC 1 cut(s) 33
Bsp1286I GDGCHC 1 cut(s) 37
Bsp1407I TGTACA 1 cut(s) 436
Bsp143I GATC 5 cut(s) 64, 168, 380, 454, 628
BspACI CCGC 4 cut(s) 450, 458, 692, 761
BspANI GGCC 3 cut(s) 17, 35, 477
BspCNI CTCAG 2 cut(s) 120, 676
BspHI TCATGA 1 cut(s) 22
BspLI GGNNCC 4 cut(s) 18, 34, 35, 214
BspMAI CTGCAG 3 cut(s) 397, 484, 568
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 3 cut(s) 375, 449, 636
BsrBI CCGCTC 1 cut(s) 763
BsrGI TGTACA 1 cut(s) 436
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 2 cut(s) 37, 951
BssMI GATC 5 cut(s) 64, 168, 380, 454, 628
BssT1I CCWWGG 2 cut(s) 37, 951
Bst4CI ACNGT 5 cut(s) 59, 85, 280, 532, 790
BstAUI TGTACA 1 cut(s) 436
BstC8I GCNNGC 1 cut(s) 462
BstDEI CTNAG 2 cut(s) 128, 684
BstF5I GGATG 3 cut(s) 189, 410, 979
BstHHI GCGC 1 cut(s) 646
BstKTI GATC 5 cut(s) 67, 171, 383, 457, 631
BstMBI GATC 5 cut(s) 64, 168, 380, 454, 628
BstMWI GCNNNNNNNGC 5 cut(s) 41, 241, 641, 689, 769
BstSFI CTRYAG 5 cut(s) 393, 480, 564, 678, 767
BstSLI GKGCMC 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 239, 550
BstV2I GAAGAC 1 cut(s) 589
BsuRI GGCC 3 cut(s) 17, 35, 477
BtsCI GGATG 3 cut(s) 189, 410, 979
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 3 cut(s) 276, 489, 786
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 462
CaiI CAGNNNCTG 1 cut(s) 452
CciI TCATGA 1 cut(s) 22
CfoI GCGC 1 cut(s) 646
Cfr13I GGNCC 5 cut(s) 16, 33, 34, 476, 758
Csp6I GTAC 1 cut(s) 437
CviAII CATG 5 cut(s) 23, 231, 618, 881, 935
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 2 cut(s) 128, 684
DpnI GATC 5 cut(s) 66, 170, 382, 456, 630
DpnII GATC 5 cut(s) 64, 168, 380, 454, 628
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
Eco130I CCWWGG 2 cut(s) 37, 951
Eco24I GRGCYC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 758
EcoO109I RGGNCCY 3 cut(s) 16, 33, 34
EcoT14I CCWWGG 2 cut(s) 37, 951
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 37, 951
FaeI CATG 5 cut(s) 26, 234, 621, 884, 938
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FaqI GGGAC 1 cut(s) 884
FatI CATG 5 cut(s) 22, 230, 617, 880, 934
Fnu4HI GCNGC 4 cut(s) 228, 450, 564, 693
FokI GGATG 3 cut(s) 196, 417, 966
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 4 cut(s) 228, 450, 564, 693
FspBI CTAG 3 cut(s) 38, 852, 909
FspI TGCGCA 1 cut(s) 645
GlaI GCGC 1 cut(s) 645
GluI GCNGC 4 cut(s) 228, 450, 564, 693
HaeIII GGCC 3 cut(s) 17, 35, 477
HapII CCGG 1 cut(s) 216
HhaI GCGC 1 cut(s) 646
Hin1II CATG 5 cut(s) 26, 234, 621, 884, 938
Hin6I GCGC 1 cut(s) 644
HinP1I GCGC 1 cut(s) 644
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 4 cut(s) 296, 389, 799, 805
HpaII CCGG 1 cut(s) 216
HphI GGTGA 5 cut(s) 63, 68, 73, 250, 423
Hpy166II GTNNAC 3 cut(s) 287, 610, 823
Hpy188I TCNGA 5 cut(s) 64, 129, 628, 804, 960
Hpy188III TCNNGA 4 cut(s) 23, 172, 284, 535
Hpy8I GTNNAC 3 cut(s) 287, 610, 823
HpyAV CCTTC 6 cut(s) 184, 394, 404, 481, 646, 912
HpyCH4III ACNGT 5 cut(s) 59, 85, 280, 532, 790
HpyCH4V TGCA 4 cut(s) 395, 482, 566, 970
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 241, 641, 689, 769
HpyF3I CTNAG 2 cut(s) 128, 684
Hsp92II CATG 5 cut(s) 26, 234, 621, 884, 938
HspAI GCGC 1 cut(s) 644
Kzo9I GATC 5 cut(s) 64, 168, 380, 454, 628
LmnI GCTCC 3 cut(s) 218, 332, 768
Lsp1109I GCAGC 2 cut(s) 239, 550
LweI GCATC 1 cut(s) 437
MaeI CTAG 3 cut(s) 38, 852, 909
MaeIII GTNAC 4 cut(s) 202, 218, 730, 937
MalI GATC 5 cut(s) 66, 170, 382, 456, 630
MbiI CCGCTC 1 cut(s) 763
MboI GATC 5 cut(s) 64, 168, 380, 454, 628
MboII GAAGA 4 cut(s) 386, 546, 594, 805
MfeI CAATTG 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 7 cut(s) 350, 364, 440, 471, 521, 701, 841
MlyI GAGTC 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 3 cut(s) 6, 548, 780
MslI CAYNNNNRTG 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 1 cut(s) 216
MunI CAATTG 1 cut(s) 350
MwoI GCNNNNNNNGC 5 cut(s) 41, 241, 641, 689, 769
NdeII GATC 5 cut(s) 64, 168, 380, 454, 628
NlaIII CATG 5 cut(s) 26, 234, 621, 884, 938
NlaIV GGNNCC 4 cut(s) 18, 34, 35, 214
NsbI TGCGCA 1 cut(s) 645
PagI TCATGA 1 cut(s) 22
PaqCI CACCTGC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 3 cut(s) 296, 799, 805
PkrI GCNGC 4 cut(s) 229, 451, 565, 694
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
PspN4I GGNNCC 4 cut(s) 18, 34, 35, 214
PspOMI GGGCCC 1 cut(s) 33
PspPI GGNCC 5 cut(s) 16, 33, 34, 476, 758
PstI CTGCAG 3 cut(s) 397, 484, 568
PstNI CAGNNNCTG 1 cut(s) 452
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
RseI CAYNNNNRTG 1 cut(s) 27
SaqAI TTAA 3 cut(s) 6, 548, 780
SatI GCNGC 4 cut(s) 228, 450, 564, 693
Sau3AI GATC 5 cut(s) 64, 168, 380, 454, 628
Sau96I GGNCC 5 cut(s) 16, 33, 34, 476, 758
SchI GAGTC 1 cut(s) 383
SduI GDGCHC 1 cut(s) 37
SfaNI GCATC 1 cut(s) 437
SfcI CTRYAG 5 cut(s) 393, 480, 564, 678, 767
SinI GGWCC 1 cut(s) 758
SmiMI CAYNNNNRTG 1 cut(s) 27
Sse9I AATT 7 cut(s) 350, 364, 440, 471, 521, 701, 841
SsiI CCGC 4 cut(s) 450, 458, 692, 761
SspI AATATT 1 cut(s) 964
SspMI CTAG 3 cut(s) 38, 852, 909
StyI CCWWGG 2 cut(s) 37, 951
TaaI ACNGT 5 cut(s) 59, 85, 280, 532, 790
TaqI TCGA 2 cut(s) 387, 399
TasI AATT 7 cut(s) 350, 364, 440, 471, 521, 701, 841
TatI WGTACW 1 cut(s) 436
TauI GCSGC 2 cut(s) 452, 695
TfiI GAWTC 3 cut(s) 296, 799, 805
Tru1I TTAA 3 cut(s) 6, 548, 780
Tru9I TTAA 3 cut(s) 6, 548, 780
TscAI CASTG 3 cut(s) 283, 489, 793
TseI GCWGC 2 cut(s) 227, 563
TspDTI ATGAA 2 cut(s) 39, 410
TspRI CASTG 3 cut(s) 283, 489, 793
VpaK11BI GGWCC 1 cut(s) 758
XapI RAATTY 3 cut(s) 364, 521, 701
XmaJI CCTAGG 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 3 cut(s) 38, 852, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.