MD02G1303900.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000709
Physical Location & Seq
Forward (+)
35748230 .. 35749977
1748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1303900.v1.1.491

Sequence Viewer

Length: 963 bp
ATGGATGGAGGAACGAAGGTTGTGTGTGTAACAGGAGCCTCCGGCTTCATAGCGTCGTGGCTCGTGAAGCTCTTACTACAAAGAGGTTATACTGTCAGAGCTACCGTTCGTGACCCAAATGATCCAAATAAAACAGAACACTTACTCTCACTAGATGGAGCAAAAGAACGGCTCCATTTGTTCAAAGCAGATTTACTAGAAGAAGGATCTTTCGACGCTGTAGTTGATGGATGTGTAGGTGTTTTTCATACAGCATCGCCTGCACAAATTTCAGCCATTGACCCACAGGCGGAAATAGTTGGGCCTGCAGTGAAAGGGACACTCAATGTTCTTAAATCGTGCGCGAAATTCCCCACTGTCAAGAGAGTGGTATTAACATCATCTATTGCTTCAGTAATGGGGACAGGAACTCCTCTGACATCTGATGTGGTTTTGGATGAAACATGGTACTCGGATCAACTCTTTTTCGAGAAGCACAAGCAATGGTATCCACTCTCAAAAACTTTAGCTGAGGAGGCTGCCTGGAAATTTGCTGAAGGAAATGGGATTGATTTGGTGTCGATGAATCCAGGGATGGTGATTGGTCCACTCTTCCAGCCAACTCTTAATCTCTCCGTCAAGTTTCTTCTGAATTTCATTAGTGGTGTCCAAACTCCAGTTGTAAACTACGCATTCGTGGACGTTCGAGATGTTGCCTCTGCTCATATTAAAGCATTTGAAGTTCCTTCTGCTAGTGGCAGATATTGTTTAGTTGGCCAAGTAGCTGACGGCCCCGATACTCTACAGATTTTACGACAACTTTACCCCACTTTGTGCCTTCCTGAGTTTGGCAATCCTTCAGACTCAAAGTATCAAGTGTCCAAGGAGAAAGTAAAAGGTTTGGGGATCACTTTCCTTCCTCTGGAAACAAGTCTAAGAGACACGGTTGAAAGCCTCAAGGAGAAGGGTATTCTCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

321

Amino Acids

34.69

Weight (kDa)

6.0

Isoelectric Point (pI)

22.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 101 5.8e-09 NmrA-like family
Epimerase PF01370 8 - 244 7.2e-26 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 238 8e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 200 2.2e-12 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 128 1.1e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 131 6.7e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 344
AciI CCGC 1 cut(s) 290
AclWI GGATC 4 cut(s) 116, 214, 462, 893
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 4 cut(s) 267, 347, 527, 631
AcuI CTGAAG 3 cut(s) 375, 555, 822
AdeI CACNNNGTG 1 cut(s) 813
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 3 cut(s) 289, 827, 901
AgsI TTSAA 3 cut(s) 184, 719, 929
AjnI CCWGG 2 cut(s) 521, 568
AluBI AGCT 4 cut(s) 70, 101, 509, 764
AluI AGCT 4 cut(s) 70, 101, 509, 764
Alw26I GTCTC 1 cut(s) 912
AlwI GGATC 4 cut(s) 116, 214, 462, 893
AoxI GGCC 3 cut(s) 302, 754, 769
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 4 cut(s) 267, 347, 527, 631
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 3 cut(s) 302, 584, 770
AsuHPI GGTGA 1 cut(s) 589
AvaII GGWCC 1 cut(s) 584
BalI TGGCCA 1 cut(s) 756
BauI CACGAG 1 cut(s) 62
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 505
BccI CCATC 3 cut(s) 149, 221, 568
BceAI ACGGC 2 cut(s) 185, 784
BciT130I CCWGG 2 cut(s) 523, 570
BciVI GTATCC 1 cut(s) 498
BcoDI GTCTC 1 cut(s) 912
BfaI CTAG 3 cut(s) 152, 197, 732
BfmI CTRYAG 3 cut(s) 219, 306, 782
BfuI GTATCC 1 cut(s) 498
BisI GCNGC 1 cut(s) 519
BlsI GCNGC 1 cut(s) 520
Bme1390I CCNGG 2 cut(s) 523, 570
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 3 cut(s) 302, 584, 770
BmiI GGNNCC 3 cut(s) 37, 173, 772
BmrFI CCNGG 2 cut(s) 523, 570
BmsI GCATC 1 cut(s) 263
BpmI CTGGAG 1 cut(s) 639
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 2 cut(s) 920, 938
BsaJI CCNNGG 2 cut(s) 569, 861
BsaXI ACNNNNNCTCC 2 cut(s) 394, 424
Bsc4I CCNNNNNNNGG 3 cut(s) 289, 827, 901
Bse1I ACTGG 1 cut(s) 656
Bse3DI GCAATG 1 cut(s) 488
BseBI CCWGG 2 cut(s) 523, 570
BseDI CCNNGG 2 cut(s) 569, 861
BseGI GGATG 4 cut(s) 10, 236, 442, 579
BseLI CCNNNNNNNGG 3 cut(s) 289, 827, 901
BseMI GCAATG 1 cut(s) 488
BseMII CTCAG 2 cut(s) 501, 813
BseNI ACTGG 1 cut(s) 656
BseRI GAGGAG 2 cut(s) 402, 527
BseXI GCAGC 1 cut(s) 505
BsgI GTGCAG 1 cut(s) 246
Bsh1236I CGCG 1 cut(s) 344
BshFI GGCC 3 cut(s) 304, 756, 771
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 2 cut(s) 331, 415
BslI CCNNNNNNNGG 3 cut(s) 289, 827, 901
BsmAI GTCTC 1 cut(s) 912
BsmFI GGGAC 2 cut(s) 331, 415
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 3 cut(s) 304, 756, 771
Bsp143I GATC 4 cut(s) 121, 206, 454, 885
BspACI CCGC 1 cut(s) 290
BspANI GGCC 3 cut(s) 304, 756, 771
BspCNI CTCAG 2 cut(s) 502, 814
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 3 cut(s) 37, 173, 772
BspMAI CTGCAG 1 cut(s) 310
BspPI GGATC 4 cut(s) 116, 214, 462, 893
BsrDI GCAATG 1 cut(s) 488
BsrI ACTGG 1 cut(s) 656
BssECI CCNNGG 2 cut(s) 569, 861
BssMI GATC 4 cut(s) 121, 206, 454, 885
BssSI CACGAG 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 861
Bst2BI CACGAG 1 cut(s) 62
Bst2UI CCWGG 2 cut(s) 523, 570
Bst4CI ACNGT 4 cut(s) 94, 106, 358, 925
Bst6I CTCTTC 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 2 cut(s) 261, 306
BstDEI CTNAG 3 cut(s) 510, 822, 914
BstF5I GGATG 4 cut(s) 10, 236, 442, 579
BstFNI CGCG 1 cut(s) 344
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 4 cut(s) 124, 209, 457, 888
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 4 cut(s) 121, 206, 454, 885
BstMWI GCNNNNNNNGC 3 cut(s) 67, 260, 515
BstNI CCWGG 2 cut(s) 523, 570
BstSCI CCNGG 2 cut(s) 521, 568
BstSFI CTRYAG 3 cut(s) 219, 306, 782
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 505
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BsuI GTATCC 1 cut(s) 498
BsuRI GGCC 3 cut(s) 304, 756, 771
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 4 cut(s) 10, 236, 442, 579
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 315, 354
Cac8I GCNNGC 2 cut(s) 261, 306
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 3 cut(s) 302, 584, 770
CseI GACGC 2 cut(s) 42, 224
Csp6I GTAC 1 cut(s) 448
CviAII CATG 1 cut(s) 444
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 3 cut(s) 510, 822, 914
DpnI GATC 4 cut(s) 123, 208, 456, 887
DpnII GATC 4 cut(s) 121, 206, 454, 885
DraIII CACNNNGTG 1 cut(s) 813
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
EciI GGCGGA 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 861
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 3 cut(s) 375, 555, 822
EcoRII CCWGG 2 cut(s) 521, 568
EcoT14I CCWWGG 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 1 cut(s) 447
FaiI YATR 5 cut(s) 50, 90, 249, 445, 705
FaqI GGGAC 2 cut(s) 331, 415
FatI CATG 1 cut(s) 443
Fnu4HI GCNGC 1 cut(s) 519
FokI GGATG 4 cut(s) 17, 243, 449, 586
Fsp4HI GCNGC 1 cut(s) 519
FspBI CTAG 3 cut(s) 152, 197, 732
GlaI GCGC 1 cut(s) 343
GluI GCNGC 1 cut(s) 519
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 3 cut(s) 304, 756, 771
HapII CCGG 1 cut(s) 42
HgaI GACGC 2 cut(s) 42, 224
HhaI GCGC 1 cut(s) 344
Hin1II CATG 1 cut(s) 447
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 2 cut(s) 565, 842
HpaII CCGG 1 cut(s) 42
HphI GGTGA 1 cut(s) 589
Hpy166II GTNNAC 3 cut(s) 587, 664, 679
Hpy188I TCNGA 6 cut(s) 98, 417, 424, 454, 630, 841
Hpy188III TCNNGA 8 cut(s) 64, 110, 361, 469, 686, 821, 902, 955
Hpy8I GTNNAC 3 cut(s) 587, 664, 679
Hpy99I CGWCG 2 cut(s) 58, 218
HpyAV CCTTC 8 cut(s) 10, 197, 530, 735, 827, 846, 905, 937
HpyCH4III ACNGT 4 cut(s) 94, 106, 358, 925
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 2 cut(s) 263, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 260, 515
HpyF3I CTNAG 3 cut(s) 510, 822, 914
HpySE526I ACGT 1 cut(s) 681
Hsp92II CATG 1 cut(s) 447
HspAI GCGC 1 cut(s) 342
Kzo9I GATC 4 cut(s) 121, 206, 454, 885
LmnI GCTCC 3 cut(s) 35, 158, 177
Lsp1109I GCAGC 1 cut(s) 505
LweI GCATC 1 cut(s) 263
MaeI CTAG 3 cut(s) 152, 197, 732
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 2 cut(s) 28, 110
MalI GATC 4 cut(s) 123, 208, 456, 887
MboI GATC 4 cut(s) 121, 206, 454, 885
MboII GAAGA 3 cut(s) 212, 583, 617
MflI RGATCY 1 cut(s) 206
MlsI TGGCCA 1 cut(s) 756
MluCI AATT 4 cut(s) 267, 347, 527, 631
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 1 cut(s) 836
MnlI CCTC 8 cut(s) 49, 77, 423, 505, 508, 706, 909, 944
Mox20I TGGCCA 1 cut(s) 756
MscI TGGCCA 1 cut(s) 756
MseI TTAA 4 cut(s) 333, 374, 606, 708
Msp20I TGGCCA 1 cut(s) 756
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 2 cut(s) 523, 570
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 2 cut(s) 523, 570
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 3 cut(s) 67, 260, 515
NdeII GATC 4 cut(s) 121, 206, 454, 885
NlaIII CATG 1 cut(s) 447
NlaIV GGNNCC 3 cut(s) 37, 173, 772
NmuCI GTSAC 1 cut(s) 110
PctI GAATGC 1 cut(s) 671
PfeI GAWTC 1 cut(s) 565
PkrI GCNGC 1 cut(s) 520
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 521, 568
PspGI CCWGG 2 cut(s) 521, 568
PspN4I GGNNCC 3 cut(s) 37, 173, 772
PspPI GGNCC 3 cut(s) 302, 584, 770
PstI CTGCAG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
SaqAI TTAA 4 cut(s) 333, 374, 606, 708
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 4 cut(s) 121, 206, 454, 885
Sau96I GGNCC 3 cut(s) 302, 584, 770
SchI GAGTC 1 cut(s) 836
ScrFI CCNGG 2 cut(s) 523, 570
SetI ASST 9 cut(s) 21, 72, 88, 103, 241, 511, 684, 766, 880
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 3 cut(s) 219, 306, 782
SinI GGWCC 1 cut(s) 584
SmlI CTYRAG 2 cut(s) 935, 953
SmoI CTYRAG 2 cut(s) 935, 953
Sse9I AATT 4 cut(s) 267, 347, 527, 631
SsiI CCGC 1 cut(s) 290
SspMI CTAG 3 cut(s) 152, 197, 732
StyD4I CCNGG 2 cut(s) 521, 568
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 4 cut(s) 94, 106, 358, 925
TaiI ACGT 1 cut(s) 684
TaqI TCGA 4 cut(s) 213, 468, 560, 685
TasI AATT 4 cut(s) 267, 347, 527, 631
TfiI GAWTC 1 cut(s) 565
Tru1I TTAA 4 cut(s) 333, 374, 606, 708
Tru9I TTAA 4 cut(s) 333, 374, 606, 708
TscAI CASTG 2 cut(s) 315, 361
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 518
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 5 cut(s) 37, 236, 453, 578, 625
TspGWI ACGGA 1 cut(s) 604
TspRI CASTG 2 cut(s) 315, 361
VpaK11BI GGWCC 1 cut(s) 584
XapI RAATTY 4 cut(s) 267, 347, 527, 631
XspI CTAG 3 cut(s) 152, 197, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.