Rh1DG044900

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000709
Physical Location & Seq
Forward (+)
7366372 .. 7370529
4158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG044900.1

Sequence Viewer

Length: 726 bp
ATGAGTGGAGCAAGGAAAGTTGCTGTGTGTGTAACTGGAGCGTCTGGTTATATTGCGTCATGGCTGGTCAAGCTTTTGTTAGGACGAGGTTATATTGTCAAAGCCACTGTTCGGGACCCAAACAATCCGAAGAAAACAGAACACTTGCTTTCACTTGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAAGCAGATTTGCTAGATGAAGGATCTTTTGACCCTGTGGTTGATGGATGTGACGGTGTTTTCCATACAGCATCTCCAGTACTATTTTCATCGATCAGCGACCCACAGGCAGAACTAATTGACCCTGCTGTGAAGGGAACACTAAATGTTCTGAAATCATGCAAAAAATTTCCAACCGTCAAGCGAGTGGTTTTGACATCTTCTATGGCTTCAGTAATGATGAATGGAAAACCTCTGACCCCTGATGTTGTGGTGGATGAAACATGGTATTCAGATCCCCTTATTGCTGAGAAGCACAAGTTATGGTATCTTCTTTCAAAGACTTTAGCTGAGGAGGATGCTTGGAAATTTGCTGAAGGAAATAGGATTGACTTGGTTTCAATAAATCCAGGGTTTGTGATAGGACCATTCTTACAGCCGACAATTAATTTGACCGTGGAGATGATTCAGAATCTCATAACTGGTACATACTTCAGCAATTATGTTTCGATCTCTACCTCGCATGATTTAGAGCAAAGTATGATGGAAAGTTGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

241

Amino Acids

26.52

Weight (kDa)

5.74

Isoelectric Point (pI)

23.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 8 - 106 9.5e-09 NmrA-like family
Epimerase PF01370 9 - 204 1.7e-20 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 212 2.2e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 130 3.9e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 202 8.8e-11 Male sterility protein
NAD_binding_10 PF13460 13 - 134 2.1e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 217, 455
AcsI RAATTY 2 cut(s) 353, 533
AcuI CTGAAG 3 cut(s) 381, 561, 643
AfaI GTAC 2 cut(s) 267, 652
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 3 cut(s) 187, 504, 567
AjnI CCWGG 1 cut(s) 574
AluBI AGCT 2 cut(s) 73, 515
AluI AGCT 2 cut(s) 73, 515
AlwI GGATC 2 cut(s) 217, 455
ApoI RAATTY 2 cut(s) 353, 533
ArsI GACNNNNNNTTYG 2 cut(s) 598, 630
AseI ATTAAT 1 cut(s) 612
Asp700I GAANNNNTTC 1 cut(s) 174
AspS9I GGNCC 2 cut(s) 115, 590
AvaII GGWCC 2 cut(s) 115, 590
BbvCI CCTCAGC 1 cut(s) 516
BccI CCATC 3 cut(s) 152, 224, 703
BciT130I CCWGG 1 cut(s) 576
BfaI CTAG 1 cut(s) 200
BmcAI AGTACT 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 576
Bme18I GGWCC 2 cut(s) 115, 590
BmgT120I GGNCC 2 cut(s) 115, 590
BmiI GGNNCC 2 cut(s) 116, 117
BmrFI CCNGG 1 cut(s) 576
BmsI GCATC 2 cut(s) 266, 514
BpmI CTGGAG 2 cut(s) 57, 246
Bpu10I CCTNAGC 1 cut(s) 516
Bsa29I ATCGAT 1 cut(s) 278
BsaJI CCNNGG 2 cut(s) 575, 621
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 3 cut(s) 40, 263, 652
BseBI CCWGG 1 cut(s) 576
BseCI ATCGAT 1 cut(s) 278
BseDI CCNNGG 2 cut(s) 575, 621
BseGI GGATG 3 cut(s) 239, 448, 529
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 465, 507
BseNI ACTGG 3 cut(s) 40, 263, 652
BseRI GAGGAG 1 cut(s) 533
BshVI ATCGAT 1 cut(s) 278
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 128
Bsp143I GATC 4 cut(s) 209, 279, 460, 675
BspCNI CTCAG 2 cut(s) 466, 508
BspDI ATCGAT 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 116, 117
BspPI GGATC 2 cut(s) 217, 455
BsrI ACTGG 3 cut(s) 40, 263, 652
BssECI CCNNGG 2 cut(s) 575, 621
BssMI GATC 4 cut(s) 209, 279, 460, 675
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 4 cut(s) 109, 242, 364, 622
BstDEI CTNAG 2 cut(s) 474, 516
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 3 cut(s) 239, 448, 529
BstKTI GATC 4 cut(s) 212, 282, 463, 678
BstMBI GATC 4 cut(s) 209, 279, 460, 675
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstNI CCWGG 1 cut(s) 576
BstSCI CCNGG 1 cut(s) 574
BstX2I RGATCY 2 cut(s) 209, 460
BstYI RGATCY 2 cut(s) 209, 460
Bsu15I ATCGAT 1 cut(s) 278
BsuTUI ATCGAT 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 621
BtsCI GGATG 3 cut(s) 239, 448, 529
BtsIMutI CAGTG 1 cut(s) 105
Cfr13I GGNCC 2 cut(s) 115, 590
ClaI ATCGAT 1 cut(s) 278
CseI GACGC 2 cut(s) 30, 45
Csp6I GTAC 2 cut(s) 266, 651
CviAII CATG 4 cut(s) 60, 345, 450, 689
CviJI RGCY 7 cut(s) 64, 73, 104, 175, 395, 515, 604
CviKI_1 RGCY 7 cut(s) 64, 73, 104, 175, 395, 515, 604
CviQI GTAC 2 cut(s) 266, 651
DdeI CTNAG 2 cut(s) 474, 516
DpnI GATC 4 cut(s) 211, 281, 462, 677
DpnII GATC 4 cut(s) 209, 279, 460, 675
Eco47I GGWCC 2 cut(s) 115, 590
Eco57I CTGAAG 3 cut(s) 381, 561, 643
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 574
FaeI CATG 4 cut(s) 63, 348, 453, 692
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 59, 344, 449, 688
FokI GGATG 3 cut(s) 246, 455, 536
FspBI CTAG 1 cut(s) 200
GsuI CTGGAG 2 cut(s) 57, 246
HgaI GACGC 2 cut(s) 30, 45
Hin1II CATG 4 cut(s) 63, 348, 453, 692
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 631, 637
Hpy188I TCNGA 5 cut(s) 129, 339, 423, 460, 636
Hpy188III TCNNGA 1 cut(s) 113
HpyAV CCTTC 3 cut(s) 200, 313, 536
HpyCH4III ACNGT 4 cut(s) 109, 242, 364, 622
HpyCH4V TGCA 1 cut(s) 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 2 cut(s) 474, 516
Hsp92II CATG 4 cut(s) 63, 348, 453, 692
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 209, 279, 460, 675
LmnI GCTCC 3 cut(s) 8, 38, 161
LweI GCATC 2 cut(s) 266, 514
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 2 cut(s) 31, 236
MalI GATC 4 cut(s) 211, 281, 462, 677
MboI GATC 4 cut(s) 209, 279, 460, 675
MboII GAAGA 3 cut(s) 142, 378, 488
MflI RGATCY 2 cut(s) 209, 460
MluCI AATT 7 cut(s) 303, 353, 533, 609, 613, 664, 721
MmeI TCCRAC 2 cut(s) 383, 698
MnlI CCTC 5 cut(s) 80, 429, 511, 514, 694
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 1 cut(s) 612
MspR9I CCNGG 1 cut(s) 576
MvaI CCWGG 1 cut(s) 576
MwoI GCNNNNNNNGC 1 cut(s) 70
NdeII GATC 4 cut(s) 209, 279, 460, 675
NlaIII CATG 4 cut(s) 63, 348, 453, 692
NlaIV GGNNCC 2 cut(s) 116, 117
NmuCI GTSAC 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 2 cut(s) 631, 637
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 574
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 2 cut(s) 116, 117
PspPI GGNCC 2 cut(s) 115, 590
PspPPI RGGWCCY 1 cut(s) 115
PsuI RGATCY 2 cut(s) 209, 460
RsaI GTAC 2 cut(s) 267, 652
RsaNI GTAC 2 cut(s) 266, 651
SaqAI TTAA 1 cut(s) 612
Sau3AI GATC 4 cut(s) 209, 279, 460, 675
Sau96I GGNCC 2 cut(s) 115, 590
ScaI AGTACT 1 cut(s) 267
ScrFI CCNGG 1 cut(s) 576
SetI ASST 5 cut(s) 75, 91, 421, 517, 686
SfaNI GCATC 2 cut(s) 266, 514
SinI GGWCC 2 cut(s) 115, 590
Sse9I AATT 7 cut(s) 303, 353, 533, 609, 613, 664, 721
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 574
TaaI ACNGT 4 cut(s) 109, 242, 364, 622
TaqI TCGA 2 cut(s) 278, 674
TasI AATT 7 cut(s) 303, 353, 533, 609, 613, 664, 721
TatI WGTACW 1 cut(s) 265
TfiI GAWTC 2 cut(s) 631, 637
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 5 cut(s) 167, 219, 264, 422, 459
TspRI CASTG 1 cut(s) 112
VpaK11BI GGWCC 2 cut(s) 115, 590
VspI ATTAAT 1 cut(s) 612
XapI RAATTY 2 cut(s) 353, 533
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 1 cut(s) 200
ZrmI AGTACT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.