MD02G1304400.v1.1

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000709
Physical Location & Seq
Forward (+)
35802895 .. 35806712
3818 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1304400.v1.1.491

Sequence Viewer

Length: 981 bp
ATGAGTGGAGAAACAATGGTGGTGTGTGTAACAGGAGCCTCCGGTTACATAGCATCATGGTTGGTGAAGCTCTTATTACAAGCTCTTATTACAAAGGGGTTATACTGTCAAAGCCACTGTGATCCAAAGAAAACAGAACACTTGCTGTCACTAGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAAGCAGATTTATTAGAAGAAGGATCTTTTGACACCGCAGTTGATGGATGTCAAGGCGTTTTTCATACAGTGTCTCCTGTTCAGTTTTCAGCCACTGACCCACAGGCAGAAATAGTTGAGCCCGCTGTGAAGGGAACACTTAATGTTCGAAAATCATGCACGAAATTTACTACTGTCAAGAGAGTGGTCTTAACATCTTCTATGGCTTCAGTAATTATGAGTGGAAAACCTCTGACCTCTGATGTGGTTTTTGATGAAACATGGTATTCGGATCCACTGTTTTGTGAGAAGAACAAGCAATGGTATGTACTCTCAAAAACTTTAGCTGAGGAGGCTGCCTGGAAATACGCTAAAGCAAATGGAATTGACTTGGTTACAATGAATCCAGGGTTGGTGATAGGTCCACTCTTACAGCCAACTCTTAATCTCTCCGTGAAGATGATTCAGAATCTCATAACCAGTACAGGTACCGAAACACTACCCCTTTCAAATTACCGGTTCGTTGATGTTAGGGATGTTGTCTCTGCTCATATTCTGGCATTTGAAGTTCCTTCAGCAAGTGGAAGATACTGTTTAGTTTCAAATGTTACCCACTCTTCCGAGGTTCTGAGGATTATACAAGAACTTTATCCCACTTTGCACCACCCTGAAAAATGTGAGATTGGCAGTACTTCAGACCCGATATATCAAGTATCCTCAGAGAAGGCAAAAGGTTTAGGAATTAATTTCCGTCCTTTGGAAGTAAGTCTTAGGGATACAATTGAAAGCCTGAAGGAGAAGGGCTTCCTCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

327

Amino Acids

35.85

Weight (kDa)

6.26

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 8 - 247 2.8e-21 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 242 5e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 222 2.5e-09 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 260 1.6e-07 GDP-mannose 4,6 dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 650
AciI CCGC 2 cut(s) 219, 306
AclWI GGATC 4 cut(s) 116, 214, 449, 462
AcsI RAATTY 1 cut(s) 347
AcuI CTGAAG 4 cut(s) 375, 720, 840, 974
AfaI GTAC 4 cut(s) 492, 646, 652, 853
AfiI CCNNNNNNNGG 1 cut(s) 919
AgeI ACCGGT 1 cut(s) 678
AgsI TTSAA 5 cut(s) 184, 672, 728, 765, 947
AjnI CCWGG 2 cut(s) 521, 568
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AluBI AGCT 3 cut(s) 70, 83, 509
AluI AGCT 3 cut(s) 70, 83, 509
Alw26I GTCTC 2 cut(s) 261, 709
AlwI GGATC 4 cut(s) 116, 214, 449, 462
AlwNI CAGNNNCTG 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 1 cut(s) 347
AseI ATTAAT 1 cut(s) 906
AsiGI ACCGGT 1 cut(s) 678
Asp700I GAANNNNTTC 2 cut(s) 171, 965
Asp718I GGTACC 1 cut(s) 650
AspS9I GGNCC 1 cut(s) 584
AsuHPI GGTGA 2 cut(s) 76, 589
AsuII TTCGAA 1 cut(s) 331
AvaII GGWCC 1 cut(s) 584
BamHI GGATCC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 650
BanII GRGCYC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 505
BccI CCATC 2 cut(s) 149, 221
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BciT130I CCWGG 2 cut(s) 523, 570
BciVI GTATCC 2 cut(s) 886, 931
BcoDI GTCTC 2 cut(s) 261, 709
BfaI CTAG 1 cut(s) 152
BfuI GTATCC 2 cut(s) 886, 931
BisI GCNGC 1 cut(s) 519
BlsI GCNGC 1 cut(s) 520
BmcAI AGTACT 1 cut(s) 853
Bme1390I CCNGG 2 cut(s) 523, 570
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 1 cut(s) 584
BmiI GGNNCC 3 cut(s) 37, 456, 652
BmrFI CCNGG 2 cut(s) 523, 570
BmsI GCATC 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 510
Bpu14I TTCGAA 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 956
BsaJI CCNNGG 2 cut(s) 569, 783
BsaWI WCCGGW 2 cut(s) 41, 678
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 919
Bse118I RCCGGY 1 cut(s) 678
Bse1I ACTGG 1 cut(s) 642
Bse3DI GCAATG 1 cut(s) 488
BseBI CCWGG 2 cut(s) 523, 570
BseDI CCNNGG 2 cut(s) 569, 783
BseGI GGATG 2 cut(s) 236, 703
BseLI CCNNNNNNNGG 1 cut(s) 919
BseMI GCAATG 1 cut(s) 488
BseMII CTCAG 3 cut(s) 501, 782, 894
BseNI ACTGG 1 cut(s) 642
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 1 cut(s) 505
BshNI GGYRCC 1 cut(s) 650
BshTI ACCGGT 1 cut(s) 678
BsiSI CCGG 2 cut(s) 42, 679
BslI CCNNNNNNNGG 1 cut(s) 919
BsmAI GTCTC 2 cut(s) 261, 709
Bsp119I TTCGAA 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 3 cut(s) 121, 206, 454
BspACI CCGC 2 cut(s) 219, 306
BspCNI CTCAG 3 cut(s) 502, 783, 893
BspLI GGNNCC 3 cut(s) 37, 456, 652
BspPI GGATC 4 cut(s) 116, 214, 449, 462
BspT104I TTCGAA 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 488
BsrFI RCCGGY 1 cut(s) 678
BsrI ACTGG 1 cut(s) 642
BssAI RCCGGY 1 cut(s) 678
BssECI CCNNGG 2 cut(s) 569, 783
BssMI GATC 3 cut(s) 121, 206, 454
Bst2UI CCWGG 2 cut(s) 523, 570
Bst4CI ACNGT 6 cut(s) 107, 119, 253, 358, 462, 755
Bst6I CTCTTC 1 cut(s) 784
BstBI TTCGAA 1 cut(s) 331
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 4 cut(s) 510, 791, 880, 932
BstF5I GGATG 2 cut(s) 236, 703
BstKTI GATC 3 cut(s) 124, 209, 457
BstMAI GTCTC 2 cut(s) 261, 709
BstMBI GATC 3 cut(s) 121, 206, 454
BstMWI GCNNNNNNNGC 1 cut(s) 515
BstNI CCWGG 2 cut(s) 523, 570
BstSCI CCNGG 2 cut(s) 521, 568
BstV1I GCAGC 1 cut(s) 505
BstX2I RGATCY 2 cut(s) 206, 454
BstYI RGATCY 2 cut(s) 206, 454
BsuI GTATCC 2 cut(s) 886, 931
BtsCI GGATG 2 cut(s) 236, 703
BtsIMutI CAGTG 4 cut(s) 115, 258, 276, 458
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 1 cut(s) 278
Cfr10I RCCGGY 1 cut(s) 678
Cfr13I GGNCC 1 cut(s) 584
Csp6I GTAC 4 cut(s) 491, 645, 651, 852
CspAI ACCGGT 1 cut(s) 678
CviAII CATG 3 cut(s) 57, 339, 444
CviQI GTAC 4 cut(s) 491, 645, 651, 852
DdeI CTNAG 4 cut(s) 510, 791, 880, 932
DpnI GATC 3 cut(s) 123, 208, 456
DpnII GATC 3 cut(s) 121, 206, 454
Eam1104I CTCTTC 1 cut(s) 784
EarI CTCTTC 1 cut(s) 784
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 4 cut(s) 375, 720, 840, 974
EcoRII CCWGG 2 cut(s) 521, 568
EcoT38I GRGCYC 1 cut(s) 306
FaeI CATG 3 cut(s) 60, 342, 447
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 915, 947
FatI CATG 3 cut(s) 56, 338, 443
FauI CCCGC 1 cut(s) 313
Fnu4HI GCNGC 1 cut(s) 519
FokI GGATG 2 cut(s) 243, 710
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 1 cut(s) 519
FspBI CTAG 1 cut(s) 152
GluI GCNGC 1 cut(s) 519
HapII CCGG 2 cut(s) 42, 679
Hin1II CATG 3 cut(s) 60, 342, 447
HinfI GANTC 3 cut(s) 565, 625, 631
HpaII CCGG 2 cut(s) 42, 679
HphI GGTGA 2 cut(s) 76, 589
Hpy166II GTNNAC 1 cut(s) 587
Hpy188I TCNGA 8 cut(s) 417, 424, 454, 630, 784, 792, 859, 883
Hpy188III TCNNGA 2 cut(s) 361, 973
Hpy8I GTNNAC 1 cut(s) 587
HpyAV CCTTC 6 cut(s) 197, 307, 744, 880, 949, 955
HpyCH4III ACNGT 6 cut(s) 107, 119, 253, 358, 462, 755
HpyCH4V TGCA 2 cut(s) 342, 823
HpyF10VI GCNNNNNNNGC 1 cut(s) 515
HpyF3I CTNAG 4 cut(s) 510, 791, 880, 932
Hsp92II CATG 3 cut(s) 60, 342, 447
KpnI GGTACC 1 cut(s) 654
Kzo9I GATC 3 cut(s) 121, 206, 454
LmnI GCTCC 2 cut(s) 35, 158
Lsp1109I GCAGC 1 cut(s) 505
LweI GCATC 1 cut(s) 62
MaeI CTAG 1 cut(s) 152
MaeIII GTNAC 5 cut(s) 28, 44, 147, 556, 769
MalI GATC 3 cut(s) 123, 208, 456
MboI GATC 3 cut(s) 121, 206, 454
MboII GAAGA 6 cut(s) 212, 372, 484, 631, 759, 771
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 2 cut(s) 206, 454
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 7 cut(s) 347, 396, 546, 673, 903, 907, 942
MnlI CCTC 9 cut(s) 49, 423, 430, 505, 508, 778, 786, 889, 980
MroXI GAANNNNTTC 2 cut(s) 171, 965
MseI TTAA 4 cut(s) 324, 374, 606, 906
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 2 cut(s) 42, 679
MspR9I CCNGG 2 cut(s) 523, 570
MunI CAATTG 1 cut(s) 942
MvaI CCWGG 2 cut(s) 523, 570
MwoI GCNNNNNNNGC 1 cut(s) 515
NdeII GATC 3 cut(s) 121, 206, 454
NlaIII CATG 3 cut(s) 60, 342, 447
NlaIV GGNNCC 3 cut(s) 37, 456, 652
NmuCI GTSAC 1 cut(s) 147
NspV TTCGAA 1 cut(s) 331
PdmI GAANNNNTTC 2 cut(s) 171, 965
PfeI GAWTC 3 cut(s) 565, 625, 631
PinAI ACCGGT 1 cut(s) 678
PkrI GCNGC 1 cut(s) 520
PshBI ATTAAT 1 cut(s) 906
Psp6I CCWGG 2 cut(s) 521, 568
PspGI CCWGG 2 cut(s) 521, 568
PspN4I GGNNCC 3 cut(s) 37, 456, 652
PspPI GGNCC 1 cut(s) 584
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 2 cut(s) 206, 454
RsaI GTAC 4 cut(s) 492, 646, 652, 853
RsaNI GTAC 4 cut(s) 491, 645, 651, 852
SaqAI TTAA 4 cut(s) 324, 374, 606, 906
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 3 cut(s) 121, 206, 454
Sau96I GGNCC 1 cut(s) 584
ScaI AGTACT 1 cut(s) 853
ScrFI CCNGG 2 cut(s) 523, 570
SduI GDGCHC 1 cut(s) 306
SetI ASST 9 cut(s) 72, 85, 415, 422, 511, 586, 652, 789, 898
SfaNI GCATC 1 cut(s) 62
SfuI TTCGAA 1 cut(s) 331
SinI GGWCC 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 971
SmoI CTYRAG 1 cut(s) 971
Sse9I AATT 7 cut(s) 347, 396, 546, 673, 903, 907, 942
SsiI CCGC 2 cut(s) 219, 306
SspMI CTAG 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 521, 568
TaaI ACNGT 6 cut(s) 107, 119, 253, 358, 462, 755
TaqI TCGA 1 cut(s) 331
TasI AATT 7 cut(s) 347, 396, 546, 673, 903, 907, 942
TatI WGTACW 3 cut(s) 490, 644, 851
TfiI GAWTC 3 cut(s) 565, 625, 631
Tru1I TTAA 4 cut(s) 324, 374, 606, 906
Tru9I TTAA 4 cut(s) 324, 374, 606, 906
TscAI CASTG 4 cut(s) 122, 258, 283, 465
TseFI GTSAC 1 cut(s) 147
TseI GCWGC 1 cut(s) 518
Tsp45I GTSAC 1 cut(s) 147
TspDTI ATGAA 4 cut(s) 164, 236, 453, 578
TspGWI ACGGA 2 cut(s) 604, 902
TspRI CASTG 4 cut(s) 122, 258, 283, 465
VpaK11BI GGWCC 1 cut(s) 584
VspI ATTAAT 1 cut(s) 906
XapI RAATTY 1 cut(s) 347
XmnI GAANNNNTTC 2 cut(s) 171, 965
XspI CTAG 1 cut(s) 152
ZrmI AGTACT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.