MD07G1020900.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000709
Physical Location & Seq
Reverse (-)
1769616 .. 1776499
6884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1020900.v1.1.491

Sequence Viewer

Length: 966 bp
ATGAATACCGGAGAAACAAAGACTGTATGTGTAACAGGAGCGTCTGGCTACATAGCATCATGGCTAGTGAAGCTCTTACTGCAAAAGGGTTATACCATCAAAGCCACTGTTCGCGATCCAAATGATGCAAAGAAAACGGAACACTTGCTTTCATTGGATGGAGCAAAGGAGAGGCTTCATTTGTTCAAAGCGGATTTATTAGAAGAAGGATCATTTGACGCTGTAGTTGATGGATGTGTAGGTGTTTTTCATACGGCATCGCCTGCACAAATTTCAGCCACTGACCCACAGGCAGAAATAATTGAGCCTGCAGTGAAAGGAACACTTAATGTTCTAAAATCATGTGCGAAATTCCCCGCTGTCAAGAGAGTGGTTTTAACATCTTCTGTGGCTTCAGTAAGGATGAGTGGAAAACCTCTGACCTCTGATGTGGTTTTCGACGAAACATGCTATTCGGATCCACTTTTTTGTGAGAAGACAAAGCAATGGTATCCTCTCTCAAAAATTTTAGCTGAGGAGGCCGCCTGGAAATTTGCCAAAGGAAATGGGATTGAATTGGTATCGATACATCCAGGGTTTGTGATTGGTCCACCCTTACAGCCAACTCTTAATGTCTCCATCGAGTTTCTTCTGAATATAATTAGTGGGATTGAAATACCGTTTGTAAACTACACATTTGTGGACGTTAGAGATGTTGCCTCTGCTCATATTCAAGCATTTGAAGTTCCTTCAGCGAGCGGCAGATATTGTTTAGTTGCCCACGTTGCTGACCGTACCGATACTCTAAAGATTTTACGAGAGCTTTACCCCACATTGAGCCTTCCTGAGCTTGGCAATCCTTCTGACACAAAGTTTCAAGTGTCCAGGGAGAAAGCAAAAGGTTTGGGAATCACTTTCCTTCCTCTGGAAACGAGTCTCAAGGACACTGTTGAAAGCCTCAAGGAGAAGGGCTTCCTCAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

322

Amino Acids

34.99

Weight (kDa)

6.33

Isoelectric Point (pI)

30.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 100 1.5e-09 NmrA-like family
Epimerase PF01370 9 - 245 3.5e-25 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 239 3.3e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 204 1e-11 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 185 2.1e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 132 4.7e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 738
AccII CGCG 1 cut(s) 114
AciI CCGC 4 cut(s) 191, 357, 522, 738
AclWI GGATC 4 cut(s) 110, 217, 452, 465
AcsI RAATTY 4 cut(s) 270, 350, 504, 530
AcuI CTGAAG 2 cut(s) 378, 714
AfaI GTAC 1 cut(s) 775
AfiI CCNNNNNNNGG 2 cut(s) 830, 904
AgsI TTSAA 7 cut(s) 187, 554, 653, 713, 722, 857, 932
AjnI CCWGG 3 cut(s) 524, 571, 863
AleI CACNNNNGTG 1 cut(s) 677
AluBI AGCT 4 cut(s) 73, 512, 802, 829
AluI AGCT 4 cut(s) 73, 512, 802, 829
Alw26I GTCTC 2 cut(s) 619, 920
AlwI GGATC 4 cut(s) 110, 217, 452, 465
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 1 cut(s) 519
ApoI RAATTY 4 cut(s) 270, 350, 504, 530
Asp700I GAANNNNTTC 1 cut(s) 950
AspS9I GGNCC 1 cut(s) 587
AvaII GGWCC 1 cut(s) 587
BamHI GGATCC 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 482
BbvCI CCTCAGC 1 cut(s) 513
BccI CCATC 4 cut(s) 104, 152, 224, 626
BceAI ACGGC 1 cut(s) 270
BciT130I CCWGG 3 cut(s) 526, 573, 865
BciVI GTATCC 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 619, 920
BfaI CTAG 1 cut(s) 65
BfmI CTRYAG 2 cut(s) 222, 309
BfuI GTATCC 1 cut(s) 501
BisI GCNGC 2 cut(s) 522, 739
BlsI GCNGC 2 cut(s) 523, 740
Bme1390I CCNGG 3 cut(s) 526, 573, 865
Bme18I GGWCC 1 cut(s) 587
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 1 cut(s) 459
BmrFI CCNGG 3 cut(s) 526, 573, 865
BmsI GCATC 3 cut(s) 65, 115, 266
BpiI GAAGAC 1 cut(s) 482
Bpu10I CCTNAGC 2 cut(s) 513, 825
BpuEI CTTGAG 3 cut(s) 902, 923, 941
Bsa29I ATCGAT 1 cut(s) 563
BsaJI CCNNGG 2 cut(s) 572, 864
BsaWI WCCGGW 1 cut(s) 8
Bsc4I CCNNNNNNNGG 2 cut(s) 830, 904
Bse3DI GCAATG 1 cut(s) 491
BseBI CCWGG 3 cut(s) 526, 573, 865
BseCI ATCGAT 1 cut(s) 563
BseDI CCNNGG 2 cut(s) 572, 864
BseGI GGATG 4 cut(s) 163, 239, 408, 568
BseLI CCNNNNNNNGG 2 cut(s) 830, 904
BseMI GCAATG 1 cut(s) 491
BseMII CTCAG 2 cut(s) 504, 816
BseRI GAGGAG 1 cut(s) 530
BsgI GTGCAG 1 cut(s) 249
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 521
BshVI ATCGAT 1 cut(s) 563
BsiSI CCGG 1 cut(s) 9
BslI CCNNNNNNNGG 2 cut(s) 830, 904
BsmAI GTCTC 2 cut(s) 619, 920
BsnI GGCC 1 cut(s) 521
Bsp143I GATC 3 cut(s) 115, 209, 457
Bsp68I TCGCGA 1 cut(s) 114
BspACI CCGC 4 cut(s) 191, 357, 522, 738
BspANI GGCC 1 cut(s) 521
BspCNI CTCAG 2 cut(s) 505, 817
BspDI ATCGAT 1 cut(s) 563
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 459
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 4 cut(s) 110, 217, 452, 465
BsrBI CCGCTC 1 cut(s) 738
BsrDI GCAATG 1 cut(s) 491
BssECI CCNNGG 2 cut(s) 572, 864
BssMI GATC 3 cut(s) 115, 209, 457
Bst2UI CCWGG 3 cut(s) 526, 573, 865
Bst4CI ACNGT 5 cut(s) 25, 109, 660, 773, 928
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 3 cut(s) 264, 309, 736
BstDEI CTNAG 2 cut(s) 513, 825
BstF5I GGATG 4 cut(s) 163, 239, 408, 568
BstFNI CGCG 1 cut(s) 114
BstKTI GATC 3 cut(s) 118, 212, 460
BstMAI GTCTC 2 cut(s) 619, 920
BstMBI GATC 3 cut(s) 115, 209, 457
BstMWI GCNNNNNNNGC 5 cut(s) 70, 79, 263, 518, 764
BstNI CCWGG 3 cut(s) 526, 573, 865
BstNSI RCATGY 1 cut(s) 450
BstSCI CCNGG 3 cut(s) 524, 571, 863
BstSFI CTRYAG 2 cut(s) 222, 309
BstUI CGCG 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 482
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
Bsu15I ATCGAT 1 cut(s) 563
BsuI GTATCC 1 cut(s) 501
BsuRI GGCC 1 cut(s) 521
BsuTUI ATCGAT 1 cut(s) 563
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 4 cut(s) 163, 239, 408, 568
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 4 cut(s) 105, 279, 318, 924
BtuMI TCGCGA 1 cut(s) 114
Cac8I GCNNGC 3 cut(s) 264, 309, 736
CaiI CAGNNNCTG 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 587
ClaI ATCGAT 1 cut(s) 563
CseI GACGC 2 cut(s) 30, 227
Csp6I GTAC 1 cut(s) 774
CviAII CATG 3 cut(s) 60, 342, 447
CviQI GTAC 1 cut(s) 774
DdeI CTNAG 2 cut(s) 513, 825
DpnI GATC 3 cut(s) 117, 211, 459
DpnII GATC 3 cut(s) 115, 209, 457
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 2 cut(s) 378, 714
EcoRII CCWGG 3 cut(s) 524, 571, 863
FaeI CATG 3 cut(s) 63, 345, 450
FaiI YATR 9 cut(s) 28, 53, 61, 93, 252, 343, 448, 638, 708
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FatI CATG 3 cut(s) 59, 341, 446
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 2 cut(s) 522, 739
FokI GGATG 4 cut(s) 170, 246, 415, 555
Fsp4HI GCNGC 2 cut(s) 522, 739
FspBI CTAG 1 cut(s) 65
GluI GCNGC 2 cut(s) 522, 739
HaeIII GGCC 1 cut(s) 521
HapII CCGG 1 cut(s) 9
HgaI GACGC 2 cut(s) 30, 227
Hin1II CATG 3 cut(s) 63, 345, 450
HinfI GANTC 2 cut(s) 888, 913
HpaII CCGG 1 cut(s) 9
Hpy166II GTNNAC 3 cut(s) 590, 667, 682
Hpy188I TCNGA 5 cut(s) 420, 427, 457, 633, 844
Hpy188III TCNNGA 4 cut(s) 113, 364, 824, 905
Hpy8I GTNNAC 3 cut(s) 590, 667, 682
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 6 cut(s) 200, 738, 830, 849, 908, 940
HpyCH4III ACNGT 5 cut(s) 25, 109, 660, 773, 928
HpyCH4IV ACGT 2 cut(s) 684, 762
HpyCH4V TGCA 4 cut(s) 82, 128, 266, 311
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 79, 263, 518, 764
HpyF3I CTNAG 2 cut(s) 513, 825
HpySE526I ACGT 2 cut(s) 684, 762
Hsp92II CATG 3 cut(s) 63, 345, 450
Kzo9I GATC 3 cut(s) 115, 209, 457
LmnI GCTCC 2 cut(s) 38, 161
LweI GCATC 3 cut(s) 65, 115, 266
MaeI CTAG 1 cut(s) 65
MaeII ACGT 2 cut(s) 684, 762
MaeIII GTNAC 1 cut(s) 31
MalI GATC 3 cut(s) 117, 211, 459
MbiI CCGCTC 1 cut(s) 738
MboI GATC 3 cut(s) 115, 209, 457
MboII GAAGA 4 cut(s) 215, 375, 487, 620
MflI RGATCY 1 cut(s) 457
MluCI AATT 7 cut(s) 270, 300, 350, 504, 530, 554, 639
MlyI GAGTC 1 cut(s) 922
MroXI GAANNNNTTC 1 cut(s) 950
MseI TTAA 3 cut(s) 327, 377, 609
MslI CAYNNNNRTG 1 cut(s) 677
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 3 cut(s) 526, 573, 865
MvaI CCWGG 3 cut(s) 526, 573, 865
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 5 cut(s) 70, 79, 263, 518, 764
NdeII GATC 3 cut(s) 115, 209, 457
NlaIII CATG 3 cut(s) 63, 345, 450
NlaIV GGNNCC 1 cut(s) 459
NruI TCGCGA 1 cut(s) 114
NspI RCATGY 1 cut(s) 450
OliI CACNNNNGTG 1 cut(s) 677
PdmI GAANNNNTTC 1 cut(s) 950
PfeI GAWTC 1 cut(s) 888
PkrI GCNGC 2 cut(s) 523, 740
PleI GAGTC 1 cut(s) 921
PpsI GAGTC 1 cut(s) 921
Psp6I CCWGG 3 cut(s) 524, 571, 863
PspGI CCWGG 3 cut(s) 524, 571, 863
PspN4I GGNNCC 1 cut(s) 459
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 1 cut(s) 457
RruI TCGCGA 1 cut(s) 114
RsaI GTAC 1 cut(s) 775
RsaNI GTAC 1 cut(s) 774
RseI CAYNNNNRTG 1 cut(s) 677
SaqAI TTAA 3 cut(s) 327, 377, 609
SatI GCNGC 2 cut(s) 522, 739
Sau3AI GATC 3 cut(s) 115, 209, 457
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 1 cut(s) 922
ScrFI CCNGG 3 cut(s) 526, 573, 865
SfaNI GCATC 3 cut(s) 65, 115, 266
SfcI CTRYAG 2 cut(s) 222, 309
SinI GGWCC 1 cut(s) 587
SmiMI CAYNNNNRTG 1 cut(s) 677
SmlI CTYRAG 3 cut(s) 917, 938, 956
SmoI CTYRAG 3 cut(s) 917, 938, 956
Sse9I AATT 7 cut(s) 270, 300, 350, 504, 530, 554, 639
SsiI CCGC 4 cut(s) 191, 357, 522, 738
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 3 cut(s) 524, 571, 863
TaaI ACNGT 5 cut(s) 25, 109, 660, 773, 928
TaiI ACGT 2 cut(s) 687, 765
TaqI TCGA 3 cut(s) 438, 563, 621
TasI AATT 7 cut(s) 270, 300, 350, 504, 530, 554, 639
TauI GCSGC 2 cut(s) 524, 741
TfiI GAWTC 1 cut(s) 888
Tru1I TTAA 3 cut(s) 327, 377, 609
Tru9I TTAA 3 cut(s) 327, 377, 609
TscAI CASTG 4 cut(s) 112, 286, 318, 931
TspDTI ATGAA 4 cut(s) 17, 141, 167, 239
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 4 cut(s) 112, 286, 318, 931
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 4 cut(s) 270, 350, 504, 530
XceI RCATGY 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 950
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.