Rw1G001950

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000709
Physical Location & Seq
Reverse (-)
3425618 .. 3428218
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G001950.1

Sequence Viewer

Length: 1017 bp
ATGCGAAACATCCATCTCAGCGCACAAAATCATTGGAGGATCCGAATTCCAAACGTTGTGTGTGTTACAGGAGCTTCTGGTTTCATAGCTTCATGGCTGGTGAAGCTCTTGTTACAGCGAGGTTATGTCGTCAAAGCCACCGTTCGTGACCCAAATAATCCGAAGAAAACAGAACACTTGCTCTCACTTGAGGGAGCAAAGGAAAGACTTCATTTGTTCAAAGCAGACTTGTTAGAAGAGGGGTCTTTTGACCTTGTAGTTGATGGATGTGAAGGTGTTTTCCACACAGCATCTCCGGTACTATTTTCATCGATCAGCGACCCACAGGCAGAAGTGATTGATCCTGCAGTAAAGGGAACGCTTAATGTTCTTAAATCGTGCGTGAAATTTCCAACAATCAAGAGGGTAGTTTTAACATCTTCCATGGCGGCAGTTATAGTCAATGGAAGACCTTTAACCTCTAATGTGGTAGTTGATGAAACATGGTTTTCTGATCCACTTGTTTGTCAGGAGTTGAAGCAATGGTATTTTCTTTCGAAAACTTTAGCAGAGGAGGCTGCTTGGAAATTTGCTAAAGAAAACGGGATTGACATGGTGACCATAAATCCCTCGTACGTGATGGGTCCGCCCTTGCAGCCAACTCTTAATTTGAGTGTGGAGATGGTTCTGGATCTCAAGAAGGGTACTGTCAACGGAGTAGCAATTGAAAATTATACATCTACTGATGTTAGAGATGTTGCCTCTGCTCATATTCAAGCATTTGAAGTTCCTTCCGCTAGTGGAAGATATTGTTTAGTTGCAAATGTCACCCCTATTCACAAGGCTCTGAAAATTTTAAAGGAACTTCATCCTACTTTGTGCCCACCTGAAATATGCGAGCATGACATCCCTTCCGCCCCAGAGTATCAAATATCCCAGGAAAAAGCAAAAAGTTTGGGAGTTAGTTTCCTTCCGTTGGAAGTAAGTCTCAGGGACACTGTTGAATGCCTGACGGAGAAGGGCTTCCTCAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

338

Amino Acids

37.21

Weight (kDa)

6.32

Isoelectric Point (pI)

29.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 19 - 101 6.8e-07 NmrA-like family
Epimerase PF01370 20 - 259 1.2e-21 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 22 - 254 6.3e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 22 - 216 9e-14 Male sterility protein
GDP_Man_Dehyd PF16363 22 - 209 2.9e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 24 - 145 1.2e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 428, 626, 774, 894
AclI AACGTT 1 cut(s) 54
AclWI GGATC 5 cut(s) 34, 47, 335, 488, 678
AcsI RAATTY 4 cut(s) 45, 386, 566, 831
AfaI GTAC 3 cut(s) 300, 614, 685
AfiI CCNNNNNNNGG 1 cut(s) 955
AgsI TTSAA 6 cut(s) 220, 517, 707, 755, 764, 983
AjnI CCWGG 1 cut(s) 915
AluBI AGCT 3 cut(s) 74, 89, 106
AluI AGCT 3 cut(s) 74, 89, 106
Alw26I GTCTC 1 cut(s) 971
AlwI GGATC 5 cut(s) 34, 47, 335, 488, 678
ApeKI GCWGC 2 cut(s) 557, 634
ApoI RAATTY 4 cut(s) 45, 386, 566, 831
Asp700I GAANNNNTTC 2 cut(s) 207, 1001
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 623
AsuHPI GGTGA 3 cut(s) 112, 607, 799
AsuII TTCGAA 1 cut(s) 536
AvaII GGWCC 1 cut(s) 623
BaeGI GKGCMC 1 cut(s) 863
BamHI GGATCC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 454
BbvI GCAGC 2 cut(s) 544, 646
BccI CCATC 4 cut(s) 21, 257, 613, 655
BciT130I CCWGG 1 cut(s) 917
BcoDI GTCTC 1 cut(s) 971
BfaI CTAG 1 cut(s) 777
BfmI CTRYAG 1 cut(s) 345
BisI GCNGC 3 cut(s) 429, 558, 635
BlsI GCNGC 3 cut(s) 430, 559, 636
Bme1390I CCNGG 1 cut(s) 917
Bme18I GGWCC 1 cut(s) 623
BmgT120I GGNCC 1 cut(s) 623
BmiI GGNNCC 2 cut(s) 41, 624
BmrFI CCNGG 1 cut(s) 917
BmsI GCATC 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 454
Bpu14I TTCGAA 1 cut(s) 536
BpuEI CTTGAG 3 cut(s) 209, 659, 992
Bsa29I ATCGAT 1 cut(s) 311
BsaAI YACGTR 1 cut(s) 616
BsaJI CCNNGG 2 cut(s) 423, 915
BsaWI WCCGGW 1 cut(s) 295
BsaXI ACNNNNNCTCC 2 cut(s) 277, 307
Bsc4I CCNNNNNNNGG 1 cut(s) 955
Bse3DI GCAATG 1 cut(s) 527
BseBI CCWGG 1 cut(s) 917
BseCI ATCGAT 1 cut(s) 311
BseDI CCNNGG 2 cut(s) 423, 915
BseGI GGATG 4 cut(s) 9, 272, 847, 885
BseLI CCNNNNNNNGG 1 cut(s) 955
BseMI GCAATG 1 cut(s) 527
BseMII CTCAG 2 cut(s) 31, 982
BseRI GAGGAG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 863
BseXI GCAGC 2 cut(s) 544, 646
BshVI ATCGAT 1 cut(s) 311
BsiSI CCGG 1 cut(s) 296
BsiWI CGTACG 1 cut(s) 612
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 1 cut(s) 955
BsmAI GTCTC 1 cut(s) 971
BsmFI GGGAC 1 cut(s) 986
BsmI GAATGC 1 cut(s) 989
Bsp119I TTCGAA 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 863
Bsp143I GATC 5 cut(s) 39, 312, 340, 493, 670
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 4 cut(s) 428, 626, 774, 894
BspCNI CTCAG 2 cut(s) 30, 981
BspDI ATCGAT 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 41, 624
BspMAI CTGCAG 1 cut(s) 349
BspPI GGATC 5 cut(s) 34, 47, 335, 488, 678
BspT104I TTCGAA 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 527
BssECI CCNNGG 2 cut(s) 423, 915
BssMI GATC 5 cut(s) 39, 312, 340, 493, 670
BssT1I CCWWGG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 917
Bst4CI ACNGT 3 cut(s) 142, 688, 979
Bst6I CTCTTC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 616
BstBI TTCGAA 1 cut(s) 536
BstC8I GCNNGC 1 cut(s) 878
BstDEI CTNAG 2 cut(s) 17, 968
BstDSI CCRYGG 1 cut(s) 423
BstEII GGTNACC 1 cut(s) 595
BstF5I GGATG 4 cut(s) 9, 272, 847, 885
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 5 cut(s) 42, 315, 343, 496, 673
BstMAI GTCTC 1 cut(s) 971
BstMBI GATC 5 cut(s) 39, 312, 340, 493, 670
BstMWI GCNNNNNNNGC 3 cut(s) 103, 554, 634
BstNI CCWGG 1 cut(s) 917
BstPI GGTNACC 1 cut(s) 595
BstSCI CCNGG 1 cut(s) 915
BstSFI CTRYAG 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 863
BstV1I GCAGC 2 cut(s) 544, 646
BstV2I GAAGAC 1 cut(s) 454
BstX2I RGATCY 2 cut(s) 39, 670
BstYI RGATCY 2 cut(s) 39, 670
Bsu15I ATCGAT 1 cut(s) 311
BsuTUI ATCGAT 1 cut(s) 311
BtgI CCRYGG 1 cut(s) 423
BtsCI GGATG 4 cut(s) 9, 272, 847, 885
BtsIMutI CAGTG 1 cut(s) 975
Cac8I GCNNGC 1 cut(s) 878
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 623
ClaI ATCGAT 1 cut(s) 311
Csp6I GTAC 3 cut(s) 299, 613, 684
CviAII CATG 5 cut(s) 93, 424, 483, 592, 881
CviQI GTAC 3 cut(s) 299, 613, 684
DdeI CTNAG 2 cut(s) 17, 968
DpnI GATC 5 cut(s) 41, 314, 342, 495, 672
DpnII GATC 5 cut(s) 39, 312, 340, 493, 670
DraI TTTAAA 1 cut(s) 837
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
EciI GGCGGA 2 cut(s) 615, 883
Eco130I CCWWGG 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 623
Eco91I GGTNACC 1 cut(s) 595
EcoO65I GGTNACC 1 cut(s) 595
EcoRI GAATTC 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 915
EcoT14I CCWWGG 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 5 cut(s) 96, 427, 486, 595, 884
FaqI GGGAC 1 cut(s) 986
FatI CATG 5 cut(s) 92, 423, 482, 591, 880
Fnu4HI GCNGC 3 cut(s) 429, 558, 635
FokI GGATG 3 cut(s) 279, 834, 872
Fsp4HI GCNGC 3 cut(s) 429, 558, 635
FspBI CTAG 1 cut(s) 777
GlaI GCGC 1 cut(s) 22
GluI GCNGC 3 cut(s) 429, 558, 635
HapII CCGG 1 cut(s) 296
HhaI GCGC 1 cut(s) 23
Hin1II CATG 5 cut(s) 96, 427, 486, 595, 884
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HpaII CCGG 1 cut(s) 296
HphI GGTGA 3 cut(s) 112, 607, 799
Hpy166II GTNNAC 1 cut(s) 691
Hpy188I TCNGA 4 cut(s) 44, 162, 493, 828
Hpy188III TCNNGA 5 cut(s) 146, 400, 509, 668, 676
Hpy8I GTNNAC 1 cut(s) 691
HpyAV CCTTC 6 cut(s) 266, 673, 780, 900, 959, 991
HpyCH4III ACNGT 3 cut(s) 142, 688, 979
HpyCH4IV ACGT 2 cut(s) 54, 615
HpyCH4V TGCA 3 cut(s) 347, 634, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 554, 634
HpyF3I CTNAG 2 cut(s) 17, 968
HpySE526I ACGT 2 cut(s) 54, 615
Hsp92II CATG 5 cut(s) 96, 427, 486, 595, 884
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 5 cut(s) 39, 312, 340, 493, 670
LmnI GCTCC 2 cut(s) 71, 194
Lsp1109I GCAGC 2 cut(s) 544, 646
LweI GCATC 1 cut(s) 299
MaeI CTAG 1 cut(s) 777
MaeII ACGT 2 cut(s) 54, 615
MaeIII GTNAC 5 cut(s) 64, 111, 146, 595, 805
MalI GATC 5 cut(s) 41, 314, 342, 495, 672
MboI GATC 5 cut(s) 39, 312, 340, 493, 670
MboII GAAGA 5 cut(s) 175, 248, 411, 459, 795
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 2 cut(s) 39, 670
MhlI GDGCHC 1 cut(s) 863
MluCI AATT 7 cut(s) 45, 386, 566, 646, 702, 709, 831
MmeI TCCRAC 2 cut(s) 416, 936
MroXI GAANNNNTTC 2 cut(s) 207, 1001
MseI TTAA 6 cut(s) 363, 372, 413, 455, 645, 836
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 917
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 1 cut(s) 989
MvaI CCWGG 1 cut(s) 917
MwoI GCNNNNNNNGC 3 cut(s) 103, 554, 634
NcoI CCATGG 1 cut(s) 423
NdeII GATC 5 cut(s) 39, 312, 340, 493, 670
NlaIII CATG 5 cut(s) 96, 427, 486, 595, 884
NlaIV GGNNCC 2 cut(s) 41, 624
NmuCI GTSAC 3 cut(s) 146, 595, 805
NspV TTCGAA 1 cut(s) 536
PctI GAATGC 1 cut(s) 989
PdmI GAANNNNTTC 2 cut(s) 207, 1001
Pfl23II CGTACG 1 cut(s) 612
PkrI GCNGC 3 cut(s) 430, 559, 636
Ppu21I YACGTR 1 cut(s) 616
Psp1406I AACGTT 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 915
PspEI GGTNACC 1 cut(s) 595
PspGI CCWGG 1 cut(s) 915
PspLI CGTACG 1 cut(s) 612
PspN4I GGNNCC 2 cut(s) 41, 624
PspPI GGNCC 1 cut(s) 623
PstI CTGCAG 1 cut(s) 349
PsuI RGATCY 2 cut(s) 39, 670
RsaI GTAC 3 cut(s) 300, 614, 685
RsaNI GTAC 3 cut(s) 299, 613, 684
SaqAI TTAA 6 cut(s) 363, 372, 413, 455, 645, 836
SatI GCNGC 3 cut(s) 429, 558, 635
Sau3AI GATC 5 cut(s) 39, 312, 340, 493, 670
Sau96I GGNCC 1 cut(s) 623
ScrFI CCNGG 1 cut(s) 917
SduI GDGCHC 1 cut(s) 863
SfaNI GCATC 1 cut(s) 299
SfcI CTRYAG 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 536
SinI GGWCC 1 cut(s) 623
SmlI CTYRAG 3 cut(s) 188, 674, 1007
SmoI CTYRAG 3 cut(s) 188, 674, 1007
Sse9I AATT 7 cut(s) 45, 386, 566, 646, 702, 709, 831
SsiI CCGC 4 cut(s) 428, 626, 774, 894
SspMI CTAG 1 cut(s) 777
StyD4I CCNGG 1 cut(s) 915
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 3 cut(s) 142, 688, 979
TaiI ACGT 2 cut(s) 57, 618
TaqI TCGA 2 cut(s) 311, 536
TasI AATT 7 cut(s) 45, 386, 566, 646, 702, 709, 831
TauI GCSGC 1 cut(s) 431
Tru1I TTAA 6 cut(s) 363, 372, 413, 455, 645, 836
Tru9I TTAA 6 cut(s) 363, 372, 413, 455, 645, 836
TscAI CASTG 1 cut(s) 982
TseFI GTSAC 3 cut(s) 146, 595, 805
TseI GCWGC 2 cut(s) 557, 634
Tsp45I GTSAC 3 cut(s) 146, 595, 805
TspDTI ATGAA 6 cut(s) 73, 81, 200, 297, 492, 836
TspGWI ACGGA 3 cut(s) 708, 942, 1007
TspRI CASTG 1 cut(s) 982
VpaK11BI GGWCC 1 cut(s) 623
XapI RAATTY 4 cut(s) 45, 386, 566, 831
XmnI GAANNNNTTC 2 cut(s) 207, 1001
XspI CTAG 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.